We narrowed to 34 results for: plasmids 101

...select CRISPR lentiviral plasmids. CRISPR Plasmid Kits : Find groups of plasmids that are used together ...resources or browse plasmids below by function. Addgene's CRISPR collections feature plasmids for a variety ...first CRISPR experiment. CRISPR 101 eBook : Based on our popular CRISPR 101 blog series, we've organized ...variety of factors. Browse Plasmids by Model Organism Bacteria CRISPR plasmids designed for use in bacteria.... elegans CRISPR plasmids designed for use in C. elegans . Drosophila CRISPR plasmids designed for use...Mammalian CRISPR plasmids designed for use in mammalian systems. Parasites CRISPR plasmids designed for use...parasites. Plant CRISPR plasmids designed for use in plants. Yeast CRISPR plasmids designed for use in yeast...

... Viral Plasmids Adenoviral Plasmids Adenoviral Plasmids Shuttle Plasmids Adenoviral Plasmids Adenoviral... generally need to use two plasmids: A shuttle plasmid (or transfer plasmid) containing the transgene ...Adenoviral Vector Guide All Viral Plasmids Viral Vectors 101 eBook Viral Vectors 101 YouTube Videos Addgene's ...Browse shuttle plasmids, adenoviral backbone plasmids, and bacterial strains for adenoviral vector production...for recombination with the adenoviral plasmid. An adenoviral plasmid containing the rest of the adenoviral...with the shuttle plasmid. This collection provides a curated selection of popular plasmids that can be used...Adenoviral Vector Guide Shuttle Plasmids Browse commonly used shuttle plasmids for adenoviral vector production...

...Site-Specific Recombinases Cre Plasmids Flp Plasmids Dre Plasmids Other Recombinase Plasmids Recombinase-Dependent...with GFP Plasmids 101: FLEx Vectors Optimizing AAV DIO and FLEx Vector Expression Plasmids 101: Knockout... vectors. Highlighted Plasmids Browse the tables below to find popular plasmids expressing your favorite...tamoxifen”, or “light”): Cre Recombinase Plasmids ID Plasmid Description Vector Type Promoter PI Additional...Request service for more options. Addgene blog Plasmids 101: Cre-lox Cre-ating New Methods for Site-specific...Flp Recombinase Plasmids ID Plasmid Description Vector Type Promoter PI Additional Addgene resources Search...Neuroscientist’s View Return to top Dre Recombinase Plasmids ID Plasmid Description Vector Type Promoter PI Additional...

...Adeno-Associated Viral (AAV) Plasmids AAV Transfer Plasmids AAV Packaging Plasmids AAV Helper Plasmids Adeno-associated...researchers need to use three plasmids: A transfer plasmid (or cis plasmid) containing the transgene or...and more. ID Plasmid Description PI Return to top AAV Packaging Plasmids Packaging plasmids, or RepCap ...Available AAV Packaging Plasmids This table lists all packaging plasmids (Rep/Cap plasmids) in Addgene’s repository...AAV) Vector Guide All Viral Plasmids Viral Vectors 101 eBook Viral Vectors 101 Youtube Videos Addgene's ... Viral Plasmids Adeno-Associated Viral (AAV) Plasmids Adeno-Associated...interest to introduce in cells. A packaging plasmid (or Rep/Cap plasmid) containing the AAV replication (Rep)...

...collection of SARS-CoV-2 spike plasmids, packaging plasmids, and reporter plasmids for viral pseudotyping with...Accessory Plasmids Envelope and Packaging Plasmids Popular Lentiviral Envelope and Packaging Plasmids Popular...Envelope and Packaging Plasmids Reporter Plasmids A few examples of reporter plasmids that can be used for...2 Pseudotyped Virus SARS-CoV-2 Spike Plasmids Accessory Plasmids Other Resources Mechanisms of SARS-CoV...particles See Addgene's Viral Plasmids and Resources page for more information. Plasmids Expressing Spike and ...COVID-19 and Coronavirus Plasmids & Resources Ginkgo Bioworks COVID-19 Plasmid Collection Return to top...suggestions for other plasmids that should be added to this list? Fill out our Suggest a Plasmid form or e-mail...

...Gamma-Retroviral Plasmids Gamma-Retroviral Plasmids Transfer Plasmids Packaging Plasmids Envelope Plasmids Additional...retroviral plasmids at Addgene, including popular packaging, envelope, and transfer plasmids. Viral...particles have been split into multiple plasmids for safety: Transfer plasmid — contains transgene, sgRNA, or ...flanked by LTRs Packaging plasmid — contains packaging genes Envelope plasmid — contains packaging gene...Popular Gamma-Retroviral Transfer Plasmids Want a retroviral plasmid with your gene of interest? Search...database for retroviral transfer plasmids that have inserts. ID Plasmid 5'LTR Description PI 1780 pBABE-neo...of immortalized cells. See plasmid 1771 for puromycin selection, and plasmid 1774 for neomycin selection...

...available. Browse Guides and Plasmids Lentiviral Vector Guide | Lentiviral Plasmids Lentiviral vectors are ...genes and divided into multi-plasmid systems. These systems consist of a plasmid containing the genetic information...be delivered (transfer or shuttle plasmid) and one or more plasmids containing the necessary components...packaging and envelope plasmids). When transfected into in vitro cell cultures, these plasmids direct the production...required genes are divided in the multi-plasmid system. Many viral plasmids are also self-inactivating, meaning...Browse viral vector guides and plasmids available at Addgene and other related resources. Science...Guides Viral Vectors Viral Vector Guides and Plasmids Selecting a Viral Vector Type Introduction to Viral...

...Collections INTRSECT Plasmid Collection INTRSECT Plasmid Collection INTRSECT Implementation Plasmids References ...novel INTSRECT plasmids for groups interested in producing their own INTRSECT plasmids. Online spreadsheet...Find intersectional expression plasmids that combine multiplexed recombinases, including Cre, Flp, and...that have used these constructs experimentally. Plasmids In addition to EYFP and ChR2-EYFP, a large number...rabies targeting genes. Recombinases Addgene ID Plasmid Logic Sites and Mutations In-Stock Viral Items ...None Yes Single recombinase-dependent Addgene ID Plasmid Logic Sites and Mutations In-Stock Viral Items ...recombinase-dependent: Fluorophores Addgene ID Plasmid Logic Sites and Mutations In-Stock Viral Items ...

...human cancers. Ras Pathway Plasmids Click on a name to find available plasmids for the gene, or browse the...suggestions for other plasmids that should be added to this list? Fill out our Suggest a Plasmid form or e-mail...Addgene's collection of plasmids for the Ras pathway. Ras is a small GTPase and constitutively active...mTOR Pathway p53 Pathway Background Ras Pathway Plasmids Ras Gene List Resources Background Ras is a small... - Gene List Click on a name to find available plasmids for the gene. For components with multiple subunits...World J Gastrointest Oncol. 2013 May 15; 5(5): 97–101. doi: 10.4251/wjgo.v5.i5.97. PubMed PMID: 23799159...

... Viral Plasmids Lentiviral Plasmids Lentiviral Plasmids Transfer Plasmids Packaging ...Packaging Plasmids Envelope Plasmids Resources Lentiviruses, a subset of retroviruses, are some of the most common...(a virion) are divided among multiple plasmids: Transfer plasmid — contains transgene, sgRNA, or shRNA...flanked by LTRs Packaging plasmid — contains packaging and regulatory genes; one plasmid in second-generation...second-generation, two plasmids in third-generation Envelope plasmid — contains packaging gene env This collection highlights...Guide Popular Lentiviral Transfer Plasmids Looking for lentiviral plasmids to express a CRISPR gRNA? Browse...catalog for lentiviral CRISPR plasmids for gRNA expression . ID Plasmid Generation Description PI 8453...

...and Myers Tagging Plasmids Protein Species Tag Donor Plasmid gRNA plasmid gRNA plasmid FLAG pFETCh_Donor... as the plasmids and protocols needed for using them, are described below. Read our CRISPR 101 post for.... Multiple gRNA plasmids may be used. The HDR (homology directed repair) donor plasmid with homology arms...individual tagging event. The kit contains 26 plasmids; 21 of the plasmids are for use in SapTrap reactions for...the remaining 5 plasmids include Cre and FLP expression vectors, a general cloning plasmid, and a prebuilt...suggestions for other plasmids that should be added to this list? Fill out our Suggest a Plasmid form or e-mail...CRISPR plasmids for endogenous tagging of your gene of interest. CRISPR...

...use (Link opens in a new window) . Viral Vectors 101: Systemic Capsids PHP.eB These viral vector preparations...preparations were produced with the pUCmini-iCAP-PHP.eB plasmid (Addgene #103005) . Note on PHP.eB tropism and ...preparations were produced with the pUCmini-iCAP-PHP.S plasmid (Addgene #103006) . Browse Available PHP.S AAV ...preparations were produced with the pUCmini-iCAP-PHP.V1 plasmid (Addgene #127847) . Browse Available PHP.V1 AAV...were produced with the pUCmini-iCAP-AAV.MaCPNS1 plasmid (Addgene #185136) . Browse Available MaCPNS1 AAV...were produced with the pUCmini-iCAP-AAV.MaCPNS2 plasmid (Addgene #185137) . Browse Available MaCPNS2 AAV...were produced with the pUCmini-iCAP-AAV.CAP-B10 plasmid (Addgene #175004) . Browse Available CAP-B10 AAV...

...editing CRISPR 101: Homology directed repair CRISPR 101: Validating your genomic edit CRISPR 101: Multiplex...Blog References Website CRISPR plasmids and resources at Addgene CRISPR 101 eBook (3rd edition) CRISPR software...Addgene's guide to using CRISPR plasmids in your lab, from knockouts to pooled library screening. Science... cell must be validated experimentally. Browse Plasmids: Double-Strand Break (Cut) Figure 2: Overview ...cases, delivering multiple gRNAs using a single plasmid ensures that all gRNAs will be expressed in the...likelihood that any cell containing the CRISPR plasmid will have all desired genomic edits carried out...genetic loci by cloning multiple gRNAs into a single plasmid — though some have achieved targeting in the double...

...Cell-Imaging Plasmid Collection New England Biolabs Cell-Imaging Plasmid Collection Technology Plasmids Supplemental...needed for use of these tags. Plasmids Addgene ID Plasmid Substrate Feature 101137 pSNAP-tag (T7) Vector SNAP-tag...Control Plasmid SNAP-tag Control for nuclear localization 101123 pSNAPf-ADRβ2 Control Plasmid SNAP-tag...Control Plasmid CLIP-tag Control for mitochondrial localization 101125 pCLIPf-NK1R Control Plasmid CLIP-...Control Plasmid ACP/MCP-tag Control for cell surface localization 101128 pACP-ADRβ2 Control Plasmid ACP/MCP-tag...Biolabs has deposited plasmids at Addgene to generate SNAP, CLIP, ACP or MCP tagged plasmids for visualization...localization 101129 pSNAPf-Cox8A Control Plasmid SNAP-tag Control for mitochondrial localization 101124 pSNAPf-H2B...

...Khakh Discontinued Penn Vector Core Viral Vectors (Plasmids still available, other serotypes may be available...available, click on the links for more information. Plasmids are available for all the items listed below. ...pAAV.CMV.Luc.IRES.EGFP.SV40 James M. Wilson AV-9-PV1262 Plasmid not received AV-9-PV1522 100056-AAV9 pAAV.EF1a.....ChR2-YFP.WPRE.hGH Karl Deisseroth AV-9-PV1814 Plasmid not received AV-9-PV2055 105546-AAV9 pENN.AAV.CB7...PV4703 101060-AAV1 pAAV_hsyn_NES-his-CaMPARI2-WPRE-SV40 Biosensor Eric Schreiter AV-1-PV4704 101064-AAV1... Zeng AV-2-PV0101 105530-AAV2 pAAV.CMV.PI.EGFP.WPRE.bGH Control James M. Wilson AV-2-PV0101 105530-AAV2...-WPRE-SV40 Biosensor Eric Schreiter AV-1-PV4705 101061-AAV1 pAAV_hsyn_NES-his-CaMPARI2-F391W-WPRE-SV40...

...Institute for Cell Science Plasmid Collection Allen Institute for Cell Science Plasmid Collection You May Also...full description of how to use these plasmids, check out Plasmids for Endogenous Gene Tagging in Human...Allen Institute for Cell Science has deposited plasmids to generate fluorescently tagged proteins of key...Fluorescent Protein Guide Subcellular Localization Plasmids Protocols The Allen Institute for Cell Science...induced pluripotent stem cell (hiPSC) lines. The plasmids used to generate these lines have been deposited...distribution to the research community. With these plasmids researchers will be able to generate fluorescently...Human Cells on the Addgene Blog. Plasmids for Endogenous Gene Tagging in Human Cells Find additional cell...

...Enhancer Collection Plasmids Publications Resources Enhancer AAV plasmids are plasmids which use genomic...://doi.org/10.1016/j.celrep.2021.108754 PMID: 33789096 Additional Resources Addgene Plasmid Collections...Find AAV enhancer plasmids for fluorescent labeling and recombination across brain regions and populations...) . Allen Institute for Brain Science Enhancer Plasmids The Allen Institute for Brain Science Enhancer...Enhancer Collection contains best-in-class AAV plasmids to target a variety of cell populations in different...tested in mouse brains. This collection contains plasmids for cell populations in the following regions:...Hypothalamus Cerebellum Whole brain Addgene ID Plasmid Allen Institute ID Enhancer ID FP/Recombinase Labeled...

... Plasmid Collections Immunology Research Immunology Research Plasmids and Resources You...Fluorescent Proteins Cancer Research Plasmids Gene Categories Plasmid Tables Additional Resources Mammalian...to the available plasmids for each gene. Please note that not all genes have plasmids available for request...and apoptosis. Plasmid Tables The gene names in these tables link to the available plasmids for each gene...Harnessing the Body’s Defenses to Fight Cancer Plasmids 101: Monitoring Cell Mobility Using Fluorescent ...suggestions for other plasmids that should be added to this list? Fill out our Suggest a Plasmid form or e-mail...Addgene’s immunology collection contains plasmids for genes involved in innate and adaptive immune signaling...

... Richard Kahn Lab Plasmids GTPase Plasmids GAP and GEF Plasmids References The ARF (ADP-ribosylation factor...examples of plasmid types available for each gene. Click the gene name for a full list of plasmids available...suggestions for other plasmids that should be added to this list? Fill out our Suggest a Plasmid form or e-mail...ARF family plasmids. ARF family members include regulatory GTPases, GEFs, and GAPs. Plasmid... Plasmid Collections...Collections ARF Superfamily Plasmids for ARF Superfamily Research You may also like... RAS Clone Collections Richard...

...generate stable cell lines if your plasmid contains a selection marker. Plasmid delivery is versatile thanks...Application Cas Enzyme Additional Considerations Browse Plasmids Knockout Permanently disrupt gene function; cells...different activator systems exist, including the multi-plasmid SAM system Activate Design and Clone Your gRNA ..., anneal, and insert the targeting oligos into plasmids containing the gRNA scaffold using standard restriction-ligation...review the protocol associated with the specific plasmid in question (see CRISPR protocols from Addgene ...the expression time (transient or stable). For plasmid and viral-based approaches, the species and expression...fluorophore) are also relevant. Delivery Using Plasmids The most straightforward method to deliver a CRISPR...