... pii: 56469. Edwin Chapman Neuropeptides GRAB biosensors for neuropeptides VIP, NPY, CCK, NTS, UCN, SST... orexin neuropeptides A genetically encoded sensor for in vivo imaging of orexin neuropeptides. Nat Methods...Biosensors Fluorescent biosensors are proteins designed so that their fluorescent properties are sensitive... or organisms for long-term imaging and can be designed to specifically localize to cellular compartments...compartments or organelles. Addgene’s database includes many fluorescent biosensors that signal the presence ...environmental conditions. With proper experimental design, biosensors can enable investigation of a signaling...with the viral service icon . Metal Ions Target Description Article PI Calcium jGCaMP8 Fast Genetically Encoded...

...contains many plasmid tools that were specifically designed for use in plants. These resources include tools... to fluorescent proteins. Addgene’s collection includes plasmids containing promoters that drive general... Chen. Cas9 and gRNA backbone for use in rice. Includes a protocol (Link opens in a new window) for Targeted... Yang. The MoClo CRISPR/Cas Toolkit for Plants includes CRISPR/Cas nucleases, base editors, gRNA backbones...Nekrasov. The MoClo-compatible zCas9i Cloning Kit includes a high efficiency intron-optimized SpCas9-coding...Caixia Gao. Cloning The following collections are designed for creating plant expression vectors: A modular...MoChlo: Modular Cloning Chloroplast Toolbox for design of synthetic operons for chloroplast transformation...

...Table 4. Gateway Destination Vector ID Plasmid 48222 pAC90-pmax-DEST : Gateway Destination vector derived...the dCas9 protein provides the transactivation activity, the sgRNA component provides the specificity and...and can be transferred to Gateway destination vectors (e.g., pmax-DEST [48222] and (Link opens in a new...also like... CRISPR Guide CRISPR Protocols gRNA Design Tools CRISPR Blog Posts CRISPR-on is an RNA-guided... and is designed to target promoters or enhancers of genes of interest. Different flavors of activators...new window) Lifetechnologies Gateway Destination Vectors ) to adapt to different systems by LR clonase ...clonase reaction. These plasmids are described in: Multiplexed activation of endogenous genes by CRISPR-on, ...

...the biology of C. elegans and other nematodes. Wormbuilder - Describes methods to engineer the genome of...characterized which allow researchers to express desired constructs in precise cell types and/or developmental...that contain tools for use in worm research. This includes CRISPR, RNAi, empty backbones, fluorescent proteins...expression plasmids. Large collections or kits are described below and not included in this table. For a more...search Addgene's entire collection. ID Plasmid Description Gene/Insert PI Plasmids Expressing Worm Genes...strains. CRISPR plasmids from John Calarco's lab as described in Heritable genome editing in C. elegans via ...genome-editing events. Developed in Craig Mello's lab and described in A Co-CRISPR Strategy for Efficient Genome Editing...

...Serine Phosphopeptide Library described in: Encoding human serine phosphopeptides in bacteria for proteome-...pSerOTS-C1* (V70) 188537 ). These reagents are described in detail in (Mohler et al., Mol Syst Biol 2023...purifying the phosphosite library as GST-fusion peptides (“mode #1” expression). The mode #1 phosphosite...Phosphorylated Isomers (iSPI). This new resource described in (Gassaway et al., Nature Methods 2022) is adapted...synthetic human phosphoproteome. The iSPI resource subdivides the 110,139 human phosphoserine site library ...Iterative Synthetically Phosphorylated Isomers (iSPI) described in: A multi-purpose, regenerable, proteome-scale...in a new window) rEcoli XpS and a new pSerOTS described in: System-wide optimization of an orthogonal ...

...plasmid. Cheng 17452 pLenti CMV Puro DEST 3rd Gateway destination plasmid with constitutive or inducible...lentiviral systems available at Addgene have been designed for increased researcher safety. Read our Lentiviral... are used in conjunction with the plasmid that encodes the viral genome (typically called the transfer...lentivirus technologies. ID Plasmid Generation Type Description PI 12253 pRSV-Rev 3rd Packaging Contains Rev;...for research, it is the lentiviral genome that encodes genetic material that the researcher wants delivered...table and search "lenti". ID Plasmid Generation Description PI 8453 pLKO.1 puro 3rd U6-driven shRNA empty...cloning plasmid 3rd U6-driven shRNA empty plasmid; includes a stuffer for easy cloning Root 14748 pLKO.3G ...

... Plasmid Description Industry mEmeralds Plasmid Description Industry Reds Plasmid Description Industry... Plasmid Description Industry Cyans Plasmid Description Industry Oranges Plasmid Description Industry ...Industry Greens Plasmid Description Industry Yellows Plasmid Description Industry Photoactivatables Plasmid Description... Description Industry Do you have suggestions for other plasmids that should be added to this list? Fill...

... is described in more detail in the Multiplex Assays section below. The following table describes the ... Science Guides Antibody Guide Antibody Guide View Addgene's Antibody...of B cells isolated from animals immunized as described above are used to create hybridomas, which produce...each antibody has two binding sites. Isotype Description Configuration and Valency Subclasses IgA Prevalent... used to quantify relative amounts of protein. Includes: Western blot ELISA Capture - used to isolate ...isolate specific proteins or protein complexes. Includes: Immunoprecipitation (IP) Chromatin immunoprecipitation...Visualization - used to visualize protein location. Includes: Immunofluorescence (IF) Immunohistochemistry ...

... to a genomic DNA locus and encodes a primer binding site and the desired edits on an RT template. ID ...CRISPR plasmids that have been designed for use in mammalian systems. CRISPR...Expression The following CRISPR plasmids have been designed for use in mammalian expression systems. Cut Fully...Resources CRISPR Guide Viral Preps Protocols gRNA Design Tools CRISPR Blog Posts Base Edit Catalytically... can increase transcription of a specific gene. Design your gRNA sequence to direct the dCas9-activator...gene expression by interfering with transcription. Design your gRNA to target your gene of interest’s promoter...catalytically dead dCas9 to epigenetic modifiers. Design your gRNA to target a specific promoter or enhancer...

... to a genomic DNA locus and encodes a primer binding site and the desired edits on an RT template. ID ...CRISPR plasmids that have been designed for use in bacteria. CRISPR...Bacteria The following CRISPR plasmids have been designed for use in bacteria. Cut Fully functional CRISPR/...Resources CRISPR Guide Viral Preps Protocols gRNA Design Tools CRISPR Blog Posts Base Edit Catalytically... can increase transcription of a specific gene. Design your gRNA sequence to direct the dCas9-activator...gene expression by interfering with transcription. Design your gRNA to target your gene of interest’s promoter...regardless of homology to the crRNA. This activity provides a stark contrast to Cas9 and Cpf1, which require...

...at the bottom of this page. For more in depth descriptions of each serotype, check out Addgene's blog on...Browse Available PHP.eB AAV ID Name Promoter Description Category PI Controls 28306 pAAV-FLEX-tdTomato... Browse Available PHP.S AAV ID Name Promoter Description Category PI 28306 pAAV-FLEX-tdTomato CAG tdTomato...Browse Available PHP.V1 AAV ID Name Promoter Description Category PI 104052 pAAV-CAG-DIO-EYFP CAG EYFP...Browse Available MaCPNS1 AAV ID Name Promoter Description Category PI 37825 pAAV-CAG-GFP CAG GFP Control...Browse Available MaCPNS2 AAV ID Name Promoter Description Category PI 37825 pAAV-CAG-GFP CAG GFP Control...Browse Available CAP-B10 AAV ID Name Promoter Description Category PI 37825 pAAV-CAG-GFP CAG GFP Control...

...can be designed using Benchling or using similar design tools and ordered as standard desalted oligonucleotides...phosphorylated oligonucleotides containing the guide target sequence: Up to two guides can be expressed...that utilize Golden Gate cloning to insert the desired guide RNA sequence by digestion of the backbone... #63798) Plasmids from Collection ID Plasmid Description PI Publications Kaufman, M. L., Goodson, N. B...

... Large collections or kits are described below . ID Plasmid Description Gene/Insert PI Return to top Highlights...rerio ), named for the horizontal stripes on the sides of the adult body, is a tropical freshwater fish...contain tools for use in zebrafish research. This includes plasmids for genome editing, empty backbones, ...Periklis Pantazis Lab Multibow: digital spectral barcodes for cell tracing - Sean Megason Lab Zebrabow -...Du Lab. A set of pCS2+ backbone-based Gateway destination vectors (pGCS), bearing either amino- or carboxyl-terminal...promoters, six fluorophores, and empty vectors designed to streamline the production of transgenic zebrafish...zebrafish-specific gene expression database that provides information about when genes are active in developing...

...SARS-CoV-2 Viral Pseudotyping Collection - This page includes a table of all spike expression plasmids in our... industry is continually growing. ID Plasmid Description Industry PI Return to top Other Coronavirus Plasmids... SARS-CoV, MERS, BAT-CoV Plasmids ID Plasmid Description Industry PI Due to export control regulations...plasmids related to these genes below. ID Plasmid Description Industry PI For more information on these genes...CRISPR-Cas13: Open-access SHERLOCK research protocols and design resources (Link opens in a new window) Find Zhang...COVID-19 research in the table below: ID Plasmid Description Industry PI Do you have a suggestion for plasmids...Suggest a Plasmid form. If the plasmid has been described previously we will reach out to the lab to ask...

...brain cells and circuits during behavior. Long desired by researchers, the revolutionary dynamic picture... with Addgene materials. Plasmids ID Plasmid Description PI Viral Preps Addgene distributes ready-to-use...Learn more about our viral service ID Viral Prep Description PI 65417-AAV8 pAAV-hSyn-dF-HA-KORD-IRES-mCitrine...and proximal dendrites in a viral vector Hillel Adesnik 107708-AAV9 pAAV-hSynapsin- soCoChR-GFP A somatic...nuclear mRuby3 by P2A in a viral vector Hillel Adesnik 109048-AAV9 pAAV-CAG-DIO-NLS-mRuby3-IRES-eGtACR1...ribosome entry sequence in a viral vector Hillel Adesnik 111066-AAV1 pAAV-hSyn-dLight1.1 To generate Adeno-Associated...photoswitchable control of inhibitory GPCR signaling cascades. Michael Bruchas 139505-AAV8 pAAV-Ef1a-DIO-PPO-Venus...

...Protocols gRNA Design Tools CRISPR Blog Posts The following CRISPR plasmids have been designed for use in ...CRISPR plasmids that have been designed for use in yeast. CRISPR...via homology-directed repair (HDR). ID Plasmid Description Gene/Insert Promoter Selectable Marker PI Publication... can increase transcription of a specific gene. Design your gRNA sequence to direct the dCas9-activator...gene expression by interfering with transcription. Design your gRNA to target your gene of interest’s promoter...antibody to immunoprecipitate FLAG-tagged Cas9. Design your gRNA sequence to direct dCas9 to a specific...

.... The longer pegRNA also encodes a primer binding site (PBS) and the desired edits on an RT template. ...post . Need help designing a pegRNA? Try the DeepPrime (Link opens in a new window) design tool from Hyongbum...Resources CRISPR Guide Viral Preps Protocols gRNA Design Tools CRISPR Blog Posts Prime editing is a “search...pegRNA). As with a typical gRNA, the pegRNA is designed with a spacer that binds to a specific genomic...3’ flap binds to the PBS of the pegRNA and the desired edit is incorporated into the DNA by reverse transcription...

...Plasmids - Includes tagging with mCherry, mCitrine, mCerulean Davidson Lab Plasmids - Includes many N- and...fluorescence to obtain half-maximal value. Structure describes the monomeric or oligomeric state of the protein...System Plasmids Mammalian Golenbock Lab Plasmids - Includes tagging with CFP, YFP, and mRFP Tsoulfas Lab Lentiviral...Xenopus, and C. elegans Hamdoun Lab Plasmids - Set includes tagging with mCherry, Cerulean, Citrine, and eGFP.... elegans expression Yeast Thorn Lab Vectors - Includes tagging with a wide variety of colors, some specifically...yeast Lindquist Lab Vectors - Gateway cloning; includes tagging with ECFP, EYFP, DsRed, Cerulean Bacteria...Bacteria Gradia Lab Bacterial Vectors - Includes tagging with mCherry, mOrange, mCerulean pET GFP - C-terminal...

...Protocols gRNA Design Tools CRISPR Blog Posts The following CRISPR plasmids have been designed for use in ... to a genomic DNA locus and encodes a primer binding site and the desired edits on an RT template. ID ...CRISPR expression plasmids that have been designed for use in plants. CRISPR... can increase transcription of a specific gene. Design your gRNA sequence to direct the dCas9-activator...gene expression by interfering with transcription. Design your gRNA to target your gene of interest’s promoter...

...This page lists plasmid vectors designed to express nucleases in different cell types. Zinc finger arrays...Expression Vectors This page lists plasmid vectors designed to express nucleases in different cell types. ...zebrafish (can be used as a substitute for pMLM335 described in Foley et al., PLoS One 2009) pMLM292 (for expression...zebrafish (can be used as a substitute for pMLM336 described in Foley et al., PLoS One 2009) pMLM800 (for expression...Methods 2011 ) in mammalian cells or zebrafish as described by Moore et al. PLoS One 2012 and Cade et al.,...