We narrowed to 30 results for: N-pac
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TypeCollection...contains several functional domains, including: the N - t erminal D omain (NTD) the M ethyl B inding D omain...Tissue Bank Antibodies (Link opens in a new window) N-terminal Anti-MECP2 (M7443) mouse antibody from Sigma...trans-splicing, and protein replacement to reverse the impacts of this disease. Addgene is working with the RSRT...
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Tetracycline Inducible Expression
TypeCollection...PMID: 7792603 (Link opens in a new window) Heinz, N., Schambach, A., Galla, M., Maetzig, T., Baum, C.,...15517964 (Link opens in a new window) Loew, R., Heinz, N., Hampf, M., Bujard, H., & Gossen, M. (2010). Improved...transactivators and inducible tools or consider our AAV Packaged on Request service available for many transactivator...H., & Hillen, W. (2000). Exploring the sequence space for tetracycline-dependent transcriptional activators... -
p53 Pathway
TypeCollection...SD, Venkatachalam S, Choi J, Jones S, Ghebranious N, Igelmann H, Lu X, Soron G, Cooper B, Brayton C, Park...weight (53 kDa) when run on an SDS-PAGE gel. Cancer Impact p53 is the most frequently mutated gene (>50%) ...disorder known as Li-Fraumeni syndrome. Recently some impacts of p53 mutations have been characterized as gain-of-function... -
TALEN Guide
TypeCollection...149-53. PMID: 21248753 . TALEs for the masses. Rusk N. Nat Methods 2011 Mar;8(3):197. Pubmed PMID: 21473016...FokI nuclease operates as a dimer, cutting in the spacer region between two distinct ZF target sites. (B... -
Bacterial Expression Systems
TypeCollection... (vitamin B12) Escherichia coli Remy Loris 47663 pAC-EsaR-I70V Plac 3OC6HSL (Quorum sensing molecule) ...BiFC Lynne Regan 168257 168472 pMRBad-C-wtBlc pET11a-N-DiB2 DiB2 (reconstructed) BiFC Jens Meiler Additional... direct recombinant proteins to the periplasmic space between the inner and outer membranes. Targeting... 113634 pSCrhaB2 PrhaB Rhamnose Burkholderia cenocepacia Miguel Valvano 47640 pCS-PesaRlux PesaR 3OC6HSL... -
Lentivirus Plasmids
TypeCollection...David Rawlings and Andrew Scharenberg 22502 pCEP4-tat N/A Expression of tat . Increases viral titer. Sergey...shRNA of interest flanked by LTRs Packaging plasmid — contains packaging and regulatory genes; one plasmid...Addgene lentiviral packaging, envelope, and transfer plasmids Viral...Lentiviral Plasmids Transfer Plasmids Packaging Plasmids Envelope Plasmids Resources Lentiviruses, a subset... third-generation Envelope plasmid — contains packaging gene env This collection highlights popular second...second- and third-generation lentiviral packaging, envelope, and transfer plasmids available at Addgene.... Feng Zhang Return to top Popular Lentiviral Packaging Plasmids ID Plasmid Generation Description PI ... -
Immunology Research Plasmids and Resources
TypeCollection...ATF2, HFB1-GDNF GH1 growth hormone 1 GH, GH-N, GHN, hGH-N GH2 growth hormone 2 GH-V, GHL, GHV, hGH-V GHR... TRUSS UBR1 ubiquitin protein ligase E3 component n-recognin 1 JBS, MGC142065, MGC142067 UBXN1 UBX domain..., C-HA-RAS1, CTLO, H-RASIDX, HAMSV, HRAS1, K-RAS, N-RAS, RASH1 IFITM1 interferon induced transmembrane...neuroblastoma RAS viral (v-ras) oncogene homolog ALPS4, N-ras, NRAS1 PIK3CA phosphoinositide-3-kinase, catalytic...CD331, CEK, FGFBR, FLG, FLJ99988, FLT2, HBGFR, KAL2, N-SAM, OGD FGFR2 fibroblast growth factor receptor 2...NT4, NT5, NTF5 NTS neurotensin NMN-125, NN, NT, NT/N, NTS1 NUDT6 nudix (nucleoside diphosphate linked moiety...SDC2 syndecan 2 HSPG, HSPG1, SYND2 SDC3 syndecan 3 N-syndecan, SDCN, SYND3 SDC4 syndecan 4 MGC22217, SYND4... -
Cre-Lox and Other Site-Specific Recombinases
TypeCollection... proteins: The recombinase is split into inactive N- and C-terminal fragments and placed under the control...ATAACTTCGTATAgcatacatTATACGAAGTTAT 13 bp inverted repeats + 8 bp spacer (34 bp) lox2272, lox5171, lox511, loxN, lox66/lox71...TAACTTTAAATAATgccaATTATTTAAAGTTA 14 bp inverted repeats + 4 bp spacer (32 bp) Currently, only the canonical rox site ...GAAGTTCCTATTCCGAAGTTCCTATTCtctagaaaGTATAGGAACTTC 13 bp inverted repeats + 8 bp spacer (48 bp or 34 bp) F3, F5, FRT14, FRT15 minimal FRT...feature Cre-dependent tools, or consider our AAV Packaged on Request service for more options. Addgene blog...feature Flp-dependent tools, or consider our AAV Packaged on Request service for more options. Addgene blog... -
Plasmids for Stem Cell Research
TypeCollection...Klf4, and c-Myc from polycistronic cassettes KLF4 N-terminal variance modulates induced reprogramming ...are proliferative, unspecialized cells with the capacity to differentiate into many different cell types...every other cell type in the body and have the capacity to give rise to an entire organism. iPSC technology... -
Genetic Code Expansion
TypeCollection...Hang 126035 pEVOL-ABK PylRS M. barkeri 3’-azibutyl-N-carbamoyl-lysine (AbK) Bacterial TAG Andrea Musacchio...Huiwang Ai 73544 pEvol-pAcFRS.2.t1 pAcFRS.2.t1 E. coli p-acetyl-l-phenylalanine (pAcF) Bacterial TAG Farren... Isaacs 73545 pEvol-pAcFRS.1.t1 pAcFRS.1.t1 E. coli p-acetyl-l-phenylalanine (pAcF) Bacterial TAG Farren...141173 pAcBac1-3nitroY-A7-RS A7 3nY tRNA synthetase M. barkeri Mammalian Ryan Mehl 141174 pAcBac1-haloTyrRS...acid Bacerial, Mammalian TAG Peter Schultz 50831 pAcBac2.tR4-OMeYRS/GFP* tyrosyl-tRNA synthetase E. coli... amino acids Mammalian TAG Peter Schultz 50832 pAcBac1.tR4-MbPyl PylRS M. barkeri variety of unnatural...barkeri Tetrazine 3.0 Bacterial TAG Ryan Mehl 174081 pAcBac1-NES-Flag-R2-84-MbRS Tetrazine3.0 NES-Flag-R2-84...