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We narrowed to 115 results for: REP

Showing: 21 - 40 of 115 results

  1. Deisseroth INTRSECT Collection

    Type
    Collection
    ...Reveals Local and Long-Range Circuit Motifs. Cell Rep. 28(13):3450-3461. PubMed (Link opens in a new window...novel population of long-range inhibitory neurons. Preprint (Link opens in a new window) Yu K, Ahrens S, Zhang...

  2. CRISPR History and Development for Genome Engineering

    Type
    Collection
    ...Mapping Multiprotein Complexes in Human Cells. Cell Rep . 13(3):621-33. PMID: 26456817 Fu Y, Sander JD, Reyon...other hand, if a repair template with homology arms was supplied, the break could be repaired according to...loci. Activate or Repress : dCas9 fused to transcriptional activators or repressors can modulate transcription...mouse, and rat) Bacteria ( E. coli , Streptococcus , Streptomyces , and others) Drosophila Plants (monocots...Clustered Regularly Interspaced Short Palindromic Repeat) sequences were initially discovered in the E. ...Protospacers are separated by short palindromic repeat sequences. (1) The CRISPR array is transcribed ...tracrRNA) with homology to the short palindromic repeat. The tracrRNA helps recruit RNAse III and Cas9 ...

  3. CRISPR Guide

    Type
    Collection
    ...sequence. The resulting DSB is then repaired by one of two general repair pathways: the efficient but error-prone...directed repair (HDR) pathway. HDR relies on copying DNA from a matching template to accurately repair or fill... NHEJ is a naturally occurring repair pathway and is the primary repair mechanism for DSBs. While this...base excision repair pathway, which fully removes the uridine from the DNA and replaces it with another... tool to activate, repress, visualize, and isolate genes. Activation or Repression of Target Genes Targeting...sufficient to repress transcription by blocking initiation. More robust transcriptional repression (CRISPR ...demethylation DNMT3A — repression through cytosine methylation MQ1 — repression through cytosine methylation...

  4. Luciferase Plasmid Collection

    Type
    Collection
    ... 64034 pGL4Luc-RLuc Firefly, Renilla Dual reporter reporter vector allowing the study of biscistronic ...Multiple Multi-luciferase reporter vector including five transcriptional reporters for NF-kb, TGF-b, c-Myc...for gene expression assays and bioluminescent reporters. Plasmid...Collection Empty Backbones Expression Constructs Reporter Constructs Other Highlights Additional Resources...-lives, making them useful as transcriptional reporters, but are less-than-ideal protein tags due to their...or gene of interest into to create a luciferase reporter. Unless otherwise noted, these plasmids are meant...luciferase and renilla_polyA Firefly, Renilla Dual reporter vector. Insertion of 5' promoter/enhancer regions...

  5. Bacterial Expression Systems

    Type
    Collection
    ...Promoters Repressible Promoters Synthetic Photobiology: Optogenetics for E. coli Return to top Reporter Plasmids...proteins and specific DNA sequences. ID Plasmid Reports Reporter Mechanism Expression Species PI 14474 pRU1161...protein interactions, controlled gene expression, reporter plasmids and genome engineering of bacteria. Plasmid...Visualization Protein Interactions Controlled Expression Reporter Plasmids Genome Engineering You may also like…...Bacteria are commonly used to create, store, and replicate plasmids of all types, but beyond that, researchers...signal peptides include: Epitope tags: 6xHis, Flag, Strep II, c-Myc, HA, V5, GST Solubility tags: MBP, SUMO...Plasmid Series contains plasmids suitable for replication in many gram-negative bacteria with different...

  6. AAV Packaged on Request

    Type
    Collection
    ...While our repository serves the needs of many researchers, scientists sometimes require preps beyond our...service with all-in-one pricing for viral vector preps, which includes MTA facilitation, DNA amplification...protection levels. We offer 1,077 in-stock viral vector preps, delivering high quality and ready availability ...start experiments as soon as they receive their AAV prep from Addgene — eliminating the steps to produce,...recombinase, and biosensor transfer plasmids in our repository are eligible for this service. Select any eligible...eligible plasmid in the Addgene repository in your choice of seven serotypes and four volumes. Details ... ordered in four sizes. For all viral vector preparations, we set both minimum standards and typical titers...

  7. Neurodegeneration Plasmid Collection

    Type
    Collection
    ...pcDNA3.1(+)_Strep-Lrrk2-Myc LRRK2 Myc, Strep CMV Parkinson's Maik Hintze 161583 pPuro3.1(+)_Strep-Lrrk2-Myc...Myc, Strep CMV Parkinson's Maik Hintze 161584 pPuro3.1(+)_Strep-Lrrk2(D1994S)-Myc LRRK2 Myc, Strep CMV ...pPuro3.1(+)_Strep-Lrrk2(G2019S)-Myc LRRK2 Myc, Strep CMV Parkinson's Maik Hintze 161586 pPuro3.1(+)_Strep-Lrrk2...A11(2-196)-Strep ANXA11 His, Strep, Tev T7 ALS Lalit Deshmukh 196209 GB1-A11(2-196, G38R)-Strep ANXA11 His... His, Strep, Tev T7 ALS Lalit Deshmukh 196210 GB1-A11(2-196, D40G)-Strep ANXA11 His, Strep, Tev T7 ALS... G175R)-Strep ANXA11 His, Strep, Tev T7 ALS Lalit Deshmukh 196212 GB1-A11(2-196, G189E)-Strep ANXA11 His... His, Strep, Tev T7 ALS Lalit Deshmukh 196213 GB1-A11(2-52)-Strep ANXA11 His, Strep, Tev T7 ALS Lalit ...

  8. Cre-Lox and Other Site-Specific Recombinases

    Type
    Collection
    ...viral preps expressing Cre and other site-specific recombinases. Many of our in-stock viral preps feature...viral preps expressing Flp and other site-specific recombinases. Many of our in-stock viral preps feature...Plasmids Recombinase-Dependent Empty Backbones Reporter Plasmids Pooled Libraries/Kits Site-specific recombinase...active site. PhiC31 integrase, derived from a Streptomyces phage, and Bxb1 integrase, from a mycobacteriophage...recombinases , as well as useful empty backbones or reporters . Experimental Considerations Recombinase Recognition...ATAACTTCGTATAgcatacatTATACGAAGTTAT 13 bp inverted repeats + 8 bp spacer (34 bp) lox2272, lox5171, lox511,...TAACTTTAAATAATgccaATTATTTAAAGTTA 14 bp inverted repeats + 4 bp spacer (32 bp) Currently, only the canonical...

  9. Rett Syndrome

    Type
    Collection
    ... RNA editing, gene replacement therapy, RNA trans-splicing, and protein replacement to reverse the impacts...suggested roles in transcriptional repression, activity dependent de-repression, chromatin remodeling, gene ...Cell Line Repositories (Link opens in a new window) Coriell Institute - cell line repository, including...in a new window) MMRRC Repository - NIH-supported mouse and cell line repository (Link opens in a new window...largest biorepositories (Link opens in a new window) ATCC - the world's largest cell line repository Tissue...spoken language and hand skills, development of repetitive hand stereotypies, and gait dysfunction or absence...to bind to methylated DNA and recruit known co-repressors or other transcriptional modifiers suggests the...

  10. Tetracycline Inducible Expression

    Type
    Collection
    ...known as the tTA-dependent or tet-repressible system (or dox-repressible system). Tetracycline On (Tet-On...important for tetracycline-dependent repression led to a reverse Tet repressor, or rTetR, which specifically ...dox-regulated plasmids . Looking for viral preps? Find in-stock viral preps of tetracycline transactivators and... Plasmids Additional Resources Inducible and repressible promoters are powerful tools to control gene ... eukaryotes. To avoid secondary effects from repurposing endogenous systems that respond to natural stimuli...resistance operon from E. coli : the tetracycline repressor protein (TetR) and operator ( tet O, a 19 nucleotide... its activation domain than simply removing a repressor. Tet-On has since become one of the most popular...

  11. TALEN Plasmids and Kits

    Type
    Collection
    ...generate TALORs (TAL Orthongal Repressors) that can be used to custom repress gene expression in yeast. TALORs... customizable arrays of polymorphic amino acid repeats in the TAL effectors. View Addgene's TALEN Guide...efficiently assemble TALEN constructs with custom repeat arrays. TALEN Kits TALEN Kit Description Depositing...along with strong nuclear localisation tags and repress transcription initiation when targeted to DNA sequences... pFUS array vectors, destination vectors and a reporter vector for mammalian cell-based validation assay...N- and C-terminal domains (N153AA, C47AA). The reporter vector pGL4-SSA can be used for single-strand ... TALEs), 63 wild-type TAL amino acids after the repeat domain, and C-terminal SV40 NLS and HA tags. Plasmid...

  12. Empty Backbones - Choosing Your Perfect Plasmid Backbone

    Type
    Collection
    ...modification, reporter assays, shRNA expression, transgenics, and more. Element Details Representative Empty ...luciferase reporter Promoter Measure promoter strength pBV-Luc - Luciferase reporter plasmid...plasmids containing only the elements needed for replication in the host cell and are designed for a user ...host organism. Host Relevant Promoters Representative Empty Backbones Mammalian CMV, SV40, EF1a, ...Tag or Fusion Protein Common uses Representative Empty Backbones Flag Epitope tag...expression (Gateway) pYIC - Bicistronic fluorescent reporter gene with cap-dependent 3Myc-EYFP-HA-His6 and ...for viral vector delivery, genome modification, reporter assays, shRNA expression, transgenics, and more...

  13. Caltech Systemic Capsids

    Type
    Collection
    ..., Addgene is distributing ready-to-use viral preparations in engineered systemic serotypes from the Viviana...and full sequencing of the final viral vector preparation. Systemic Delivery AAV Capsid Choice Need help...Addgene’s Data Hub and the Caltech CLOVER Center’s repository of published capsid use (Link opens in a new ...: Systemic Capsids PHP.eB These viral vector preparations were produced with the pUCmini-iCAP-PHP.eB plasmid...tropism exhibited by the PHP.eB serotype has been reported to occur by using the cellular receptor LY6A (...expression iCre AIBS , Ting PHP.S These viral vector preparations were produced with the pUCmini-iCAP-PHP.S plasmid...tdTomato Control Boyden PHP.V1 These viral vector preparations were produced with the pUCmini-iCAP-PHP.V1 plasmid...

  14. Plan Your Experiment

    Type
    Collection
    ...genome editing. Scientific Reports , 5 , 11315. https://doi.org/10.1038/srep11315 PMID: 26066060 Zukher, ...experimentally verified Cut Nick Homology Directed Repair (HDR) Generate specific genomic edits, like point...fusions with proteins that promote HDR Requires a repair template Lower editing efficiency compared to NHEJ...pegRNA optimization can be challenging Prime Edit Repress or Interfere (CRISPRi) Reduce gene expression without...sequence dCas9 or dCas9 fusion with transcriptional repressor (such as dCas9-KRAB) dCas9-KRAB can be more effective...requires donor DNA to serve as template for the repair. Once you settle on your gRNA, you can design a...target in your samples to ensure there are no discrepancies with the reference sequence you used for gRNA...

  15. Brain Initiative Collection

    Type
    Collection
    ...Plasmid Description PI Viral Preps Addgene distributes ready-to-use viral preps made from plasmids in our... BRAIN Initiative collection of plasmids, viral preps, and antibodies here. Collections...the research community. Browse plasmids , viral preps , and antibodies created with support from the BRAIN...use. Learn more about our viral service ID Viral Prep Description PI 65417-AAV8 pAAV-hSyn-dF-HA-KORD-IRES-mCitrine...plasmid encoding for Archon1 fluorescent voltage reporter, Cre-dependent expression Edward Boyden 108912...Expresses the genetically-encoded fluorescent norepinephrine(NE) sensor GRAB_NE1m in neurons Yulong Li 123308...Expresses the genetically-encoded fluorescent norepinephrine(NE) sensor GRAB_NE1m in neurons Yulong Li 123309...

  16. Genetic Code Expansion

    Type
    Collection
    ...tyrS gene knocked out, tyrTV gene replaced by tolC, and tyrU gene replaced by GentR). Expresses archaeal ...tyrTV gene replaced by tRNA variant that suppress the TAG nonsense codon, and tyrU gene replaced by tRNA ...tyrS gene knocked out, tyrTV gene replaced by tolC, and tyrU gene replaced by GentR) and release factor 1...biopolymers. Tips for Success Before beginning to reprogram the genetic code, there are several things to ... are true. Always express first with a control reporter gene: GFP for E. coli or mCherry-GFP for mammalian...RF1 function removed, with Ubiquitin-UAG-sfGFP reporter George Church 98565 C321.ΔClpS.Ub-UAG-sfGFP all...removed, ClpS inactivated, with Ubiquitin-UAG-sfGFP reporter George Church 174513 Syn61 No TCG, TCA, or TAG...

  17. TALEN Guide

    Type
    Collection
    ...amino acid repeats. These repeats only differ from each other by two amino acids, their repeat-variable ... of 18 TAL effector repeats. The kit can be used to make arrays from 12-31 repeats. Perhaps one of the... to this DNA targeting ideal. Figure 1: (A) Representation of a zinc finger (ZF) nuclease. Zinc fingers...if not slightly lower. Figure 2: Simplified representation of the Voytas/Bogdanove Golden Gate TALEN kit... would expect that assembling relatively small, repeat-laden DNA regions into a single construct would...from Addgene , custom arrays consisting of 12-31 repeats can be assembled and inserted into a variety of...

  18. SARS-CoV-2 Pseudotyped Virus

    Type
    Collection
    ...Envelope and Packaging Plasmids Reporter Plasmids A few examples of reporter plasmids that can be used for...-CoV-2 spike plasmids, packaging plasmids, and reporter plasmids for viral pseudotyping with SARS-CoV-...vesicular stomatitis virus G) are packaged as replication-defective virus particles with the SARS-CoV-2...44965 NL4-3 mCherry Luciferase Lentiviral dual reporter vector expressing mCherry and firefly luciferase...Greene 105621 Lenti-luciferase-P2A-Neo Lentiviral reporter vector expressing firefly luciferase with neomycin... pHAGE-CMV-Luc2-IRES-ZsGreen-W Lentiviral dual reporter vector expressing firefly luciferase and ZsGreen...vector expressing GFP and luciferase for use as a reporter in pseudovirus production and infection. Dapeng...

  19. Promega Plasmid Collection

    Type
    Collection
    ... and more. Luciferase Reporters Luciferase (Link opens in a new window) reporter assays use light produced...Find luciferase reporter vectors for sensitive gene expression analysis and fusion vectors containing... workflows. The collection contains luciferase reporter vectors for sensitive gene expression analysis...collection. For example, search by response element, reporter, protein, or tag, such as "NFAT", "luciferase"... a new window) is a structural complementation reporter system composed of a Large BiT (LgBiT; 18 kDa)...Addgene Find additional information about luciferase reporter assays (Link opens in a new window) from Promega...blogs for technical tips and tools for genetic reporter assays (Link opens in a new window) and NanoBRET...

  20. Brzezinski Lab CRISPR Collection

    Type
    Collection
    ...modifications: replacement of the Cbh promoter with EF1alpha addition of an mCherry reporter variant nuclear...utilizes a CRISPR/Cas9 based approach to knockout, repress, and activate gene targets. To accomplish this ...advantage of the Zhang lab all-in-one (guide/Cas9/reporter) pX458 plasmid family. They have made several ...nuclear reporters via an H2B fusion shuttle plasmid for shuttling U6-guide cassettes to make dual guide ...fusion for gene and non-coding enhancer sequence repression dCas9-VPR fusion for gene activation CRISPR/Cas9...175573 and 175574 express dCas9-KRAB-MeCP2 for repression. Plasmids 175571 and 175572 express dCas9-VPR...
Showing: 21 - 40 of 115 results