Skip to main content
Addgene

Enter a sequence to perform a BLAST-based search of Addgene plasmid sequence databases.

In Category

We narrowed to 123 results for: REP

Showing: 21 - 40 of 123 results

  1. CRISPR Plasmids - Tagging

    Type
    Collection
    ... the Doyon Lab’s publication: Dalvai et al. Cell Rep. 2015 . Alternatively, cDNAs can be cloned directly...Zebrafish Xenopus CRISPR Resources CRISPR Guide Viral Preps Protocols gRNA Design Tools CRISPR Blog Posts The... CRISPR 101 post for more information about HDR/repair template design. How to use CRISPR to tag your ...plasmids may be used. The HDR (homology directed repair) donor plasmid with homology arms and selection...transcription factors with FLAG in human cell lines. To repeat the tagging in your system, use the plasmids as...introduces an N- or C- terminal affinity tag (3xFLAG-2xSTREP) on endogenous genes for the isolation of native...the AAVS1 locus. Doyon Tagging Plasmid: 3xFLAG-2xSTREP Construct for integrating at the AAVS1 "safe harbor...

  2. Deisseroth INTRSECT Collection

    Type
    Collection
    ...Reveals Local and Long-Range Circuit Motifs. Cell Rep. 28(13):3450-3461. PubMed (Link opens in a new window...novel population of long-range inhibitory neurons. Preprint (Link opens in a new window) Yu K, Ahrens S, Zhang...

  3. CRISPR History and Development for Genome Engineering

    Type
    Collection
    ...Mapping Multiprotein Complexes in Human Cells. Cell Rep . 13(3):621-33. PMID: 26456817 Fu Y, Sander JD, Reyon...other hand, if a repair template with homology arms was supplied, the break could be repaired according to...loci. Activate or Repress : dCas9 fused to transcriptional activators or repressors can modulate transcription...mouse, and rat) Bacteria ( E. coli , Streptococcus , Streptomyces , and others) Drosophila Plants (monocots...Clustered Regularly Interspaced Short Palindromic Repeat) sequences were initially discovered in the E. ...Protospacers are separated by short palindromic repeat sequences. (1) The CRISPR array is transcribed ...tracrRNA) with homology to the short palindromic repeat. The tracrRNA helps recruit RNAse III and Cas9 ...

  4. Allen Institute for Brain Science AAV Enhancer Collection

    Type
    Collection
    ...primate neocortex. Cell Rep. 2021 Mar 30;34(13):108754. doi: 10.1016/j.celrep.2021.108754. PMID: 33789096...Link opens in a new window) . Figure 1: Selection, prep, and testing of AAV enhancers in mice. Enhancers...genetic access to cortical cell types. bioRxiv [Preprint]. 2024 Sep 26:2024.06.10.597244. doi: 10.1101/...

  5. CRISPR Guide

    Type
    Collection
    ...sequence. The resulting DSB is then repaired by one of two general repair pathways: the efficient but error-prone...directed repair (HDR) pathway. HDR relies on copying DNA from a matching template to accurately repair or fill... NHEJ is a naturally occurring repair pathway and is the primary repair mechanism for DSBs. While this...base excision repair pathway, which fully removes the uridine from the DNA and replaces it with another... tool to activate, repress, visualize, and isolate genes. Activation or Repression of Target Genes Figure...sufficient to repress transcription by blocking initiation. More robust transcriptional repression (CRISPR ...demethylation DNMT3A - repression through cytosine methylation MQ1 - repression through cytosine methylation...

  6. Luciferase Plasmid Collection

    Type
    Collection
    ... 64034 pGL4Luc-RLuc Firefly, Renilla Dual reporter reporter vector allowing the study of biscistronic ...Multiple Multi-luciferase reporter vector including five transcriptional reporters for NF-kb, TGF-b, c-Myc...for gene expression assays and bioluminescent reporters. Plasmid...Collection Empty Backbones Expression Constructs Reporter Constructs Other Highlights You may also like....-lives, making them useful as transcriptional reporters, but are less-than-ideal protein tags due to their...or gene of interest into to create a luciferase reporter. Unless otherwise noted, these plasmids are meant...luciferase and renilla_polyA Firefly, Renilla Dual reporter vector. Insertion of 5' promoter/enhancer regions...

  7. Bacterial Expression Systems

    Type
    Collection
    ...Promoters Repressible Promoters Synthetic Photobiology: Optogenetics for E. coli Return to top Reporter Plasmids...proteins and specific DNA sequences. ID Plasmid Reports Reporter Mechanism Expression Species PI 14474 pRU1161...protein interactions, controlled gene expression, reporter plasmids and genome engineering of bacteria. Plasmid...Visualization Protein Interactions Controlled Expression Reporter Plasmids Genome Engineering You may also like…...Bacteria are commonly used to create, store, and replicate plasmids of all types, but beyond that, researchers...signal peptides include: Epitope tags: 6xHis, Flag, Strep II, c-Myc, HA, V5, GST Solubility tags: MBP, SUMO...Plasmid Series contains plasmids suitable for replication in many gram-negative bacteria with different...

  8. Genomic Deletions in Mammalian Cell Lines

    Type
    Collection
    ...a maxi-prep culture. Prep size may be scaled based on the required DNA yield. Perform maxi-prep for each...colonies and inoculate into a mini-prep culture. Perform mini-prep for each sample and sequence each colony...Blog Posts Addgene is proud to present a video reprint from the Journal of Visualized Experiments (JoVE...clustered regularly interspaced short palindromic repeats (CRISPR)/CRISPR-associated (Cas) 9 system may be...DNA segment by non-homologous end joining (NHEJ) repair. Deletions have potential advantages as compared...oligos at a concentration of 100 μM in ddH 2 O. Prepare a 10 μl reaction mix for each guide and its reverse...using a Golden Gate assembly cloning strategy 3 . Prepare a 50 μl reaction mix: 100 ng circular pX330 vector...

  9. Neurodegeneration Plasmid Collection

    Type
    Collection
    ...pcDNA3.1(+)_Strep-Lrrk2-Myc LRRK2 Myc, Strep CMV Parkinson's Maik Hintze 161583 pPuro3.1(+)_Strep-Lrrk2-Myc...Myc, Strep CMV Parkinson's Maik Hintze 161584 pPuro3.1(+)_Strep-Lrrk2(D1994S)-Myc LRRK2 Myc, Strep CMV ...pPuro3.1(+)_Strep-Lrrk2(G2019S)-Myc LRRK2 Myc, Strep CMV Parkinson's Maik Hintze 161586 pPuro3.1(+)_Strep-Lrrk2...A11(2-196)-Strep ANXA11 His, Strep, Tev T7 ALS Lalit Deshmukh 196209 GB1-A11(2-196, G38R)-Strep ANXA11 His... His, Strep, Tev T7 ALS Lalit Deshmukh 196210 GB1-A11(2-196, D40G)-Strep ANXA11 His, Strep, Tev T7 ALS... G175R)-Strep ANXA11 His, Strep, Tev T7 ALS Lalit Deshmukh 196212 GB1-A11(2-196, G189E)-Strep ANXA11 His... His, Strep, Tev T7 ALS Lalit Deshmukh 196213 GB1-A11(2-52)-Strep ANXA11 His, Strep, Tev T7 ALS Lalit ...

  10. AAV Packaged on Request

    Type
    Collection
    ...While our repository serves the needs of many researchers, scientists sometimes require preps beyond our...service with all-in-one pricing for viral vector preps, which includes MTA facilitation, DNA amplification...protection levels. We offer 963 in-stock viral vector preps, delivering high quality and ready availability ...start experiments as soon as they receive their AAV prep from Addgene — eliminating the steps to produce,...recombinase, and biosensor transfer plasmids in our repository are eligible for this service. Select any eligible...eligible plasmid in the Addgene repository in your choice of: serotype (AAV1, AAV2, AAV5, AAV6, AAV8, AAV11...ordered in three sizes. For all viral vector preparations, we set both minimum standards and typical titers...

  11. Rett Syndrome

    Type
    Collection
    ... RNA editing, gene replacement therapy, RNA trans-splicing, and protein replacement to reverse the impacts...suggested roles in transcriptional repression, activity dependent de-repression, chromatin remodeling, gene ...Cell Line Repositories (Link opens in a new window) Coriell Institute - cell line repository, including...in a new window) MMRRC Repository - NIH-supported mouse and cell line repository (Link opens in a new window...largest biorepositories (Link opens in a new window) ATCC - the world's largest cell line repository Tissue...spoken language and hand skills, development of repetitive hand stereotypies, and gait dysfunction or absence...to bind to methylated DNA and recruit known co-repressors or other transcriptional modifiers suggests the...

  12. CRISPR Plasmids - Mammalian Expression

    Type
    Collection
    .... When a repair template is present, the cell may repair a DSB using homology-directed repair (HDR) instead...be used with a repair template to introduce specific edits via homology-directed repair (HDR). ID Plasmid...gRNA-defined target sequence. DSBs are preferentially repaired in the cell by non-homologous end joining (NHEJ...genomic changes, researchers use ssDNA or dsDNA repair templates with homology to the DNA flanking the...Zebrafish Xenopus CRISPR Resources CRISPR Guide Viral Preps Protocols gRNA Design Tools CRISPR Blog Posts Base...base editors convert adenine to inosine, which is replaced by guanosine to create A->G (or T->C on the opposite...nicks create a double-strand break (DSB) that is repaired using error-prone non-homologous end joining (...

  13. Tetracycline Inducible Expression

    Type
    Collection
    ...tetracycline-dependent repression. Mutating these residues led to a reverse Tet repressor, or rTetR, which ...dox-regulated plasmids . Looking for viral preps? Find in-stock viral preps of tetracycline transactivators and...also like... Blog: Inducible Promoters Blog: Repressible Promoters Mammalian shRNA Plasmids Collection...Experimental Considerations Tet Plasmids Inducible and repressible promoters are powerful tools to control gene ... eukaryotes. To avoid secondary effects from repurposing endogenous systems that respond to natural stimuli...resistance operon from E. coli : the tetracycline repressor protein (TetR) and operator ( tet O, a 19 nucleotide...Off is also known as the tTA-dependent or tet-repressible system. Tetracycline On (Tet-On) In 1995, Gossen...

  14. CRISPR Plasmids - Yeast

    Type
    Collection
    .... When a repair template is present, the cell may repair a DSB using homology-directed repair (HDR) instead...be used with a repair template to introduce specific edits via homology-directed repair (HDR). ID Plasmid...Zebrafish Xenopus CRISPR Resources CRISPR Guide Viral Preps Protocols gRNA Design Tools CRISPR Blog Posts The...gRNA-defined target sequence. DSBs are preferentially repaired in the cell by non-homologous end joining (NHEJ...genomic changes, researchers use ssDNA or dsDNA repair templates with homology to the DNA flanking the...base editors convert adenine to inosine, which is replaced by guanosine to create A->G (or T->C on the opposite...nicks create a double-strand break (DSB) that is repaired using error-prone non-homologous end joining (...

  15. TALEN Plasmids and Kits

    Type
    Collection
    ...generate TALORs (TAL Orthongal Repressors) that can be used to custom repress gene expression in yeast. TALORs... customizable arrays of polymorphic amino acid repeats in the TAL effectors. View Addgene's TALEN Guide...efficiently assemble TALEN constructs with custom repeat arrays. TALEN Kits TALEN Kit Description Depositing...along with strong nuclear localisation tags and repress transcription initiation when targeted to DNA sequences... pFUS array vectors, destination vectors and a reporter vector for mammalian cell-based validation assay...N- and C-terminal domains (N153AA, C47AA). The reporter vector pGL4-SSA can be used for single-strand ... TALEs), 63 wild-type TAL amino acids after the repeat domain, and C-terminal SV40 NLS and HA tags. Plasmid...

  16. Caltech Systemic Capsids

    Type
    Collection
    ..., Addgene is distributing ready-to-use viral preparations in engineered systemic serotypes from the Viviana...and full sequencing of the final viral vector preparation. Systemic Delivery AAV Capsid Choice Need help...Addgene’s Data Hub and the Caltech CLOVER Center’s repository of published capsid use (Link opens in a new ...: Systemic Capsids PHP.eB These viral vector preparations were produced with the pUCmini-iCAP-PHP.eB plasmid...tropism exhibited by the PHP.eB serotype has been reported to occur by using the cellular receptor LY6A (...expression iCre AIBS , Ting PHP.S These viral vector preparations were produced with the pUCmini-iCAP-PHP.S plasmid...tdTomato Control Boyden PHP.V1 These viral vector preparations were produced with the pUCmini-iCAP-PHP.V1 plasmid...

  17. Empty Backbones - Choosing Your Perfect Plasmid Backbone

    Type
    Collection
    ...modification, reporter assays, shRNA expression, transgenics, and more. Element Details Representative Empty ...luciferase reporter Promoter Measure promoter strength pBV-Luc - Luciferase reporter plasmid...plasmids containing only the elements needed for replication in the host cell and are designed for a user ...host organism. Host Relevant Promoters Representative Empty Backbones Mammalian CMV, SV40, EF1a, ...Tag or Fusion Protein Common uses Representative Empty Backbones Flag Epitope tag...expression (Gateway) pYIC - Bicistronic fluorescent reporter gene with cap-dependent 3Myc-EYFP-HA-His6 and ...for viral vector delivery, genome modification, reporter assays, shRNA expression, transgenics, and more...

  18. CRISPR Plasmids - Bacteria

    Type
    Collection
    .... When a repair template is present, the cell may repair a DSB using homology-directed repair (HDR) instead...be used with a repair template to introduce specific edits via homology-directed repair (HDR). ID Plasmid...gRNA-defined target sequence. DSBs are preferentially repaired in the cell by non-homologous end joining (NHEJ...genomic changes, researchers use ssDNA or dsDNA repair templates with homology to the DNA flanking the...Zebrafish Xenopus CRISPR Resources CRISPR Guide Viral Preps Protocols gRNA Design Tools CRISPR Blog Posts Base...base editors convert adenine to inosine, which is replaced by guanosine to create A->G (or T->C on the opposite...nicks create a double-strand break (DSB) that is repaired using error-prone non-homologous end joining (...

  19. CRISPR Plasmids - Plants

    Type
    Collection
    .... When a repair template is present, the cell may repair a DSB using homology-directed repair (HDR) instead...be used with a repair template to introduce specific edits via homology-directed repair (HDR). ID Plasmid...Zebrafish Xenopus CRISPR Resources CRISPR Guide Viral Preps Protocols gRNA Design Tools CRISPR Blog Posts The...gRNA-defined target sequence. DSBs are preferentially repaired in the cell by non-homologous end joining (NHEJ...genomic changes, researchers use ssDNA or dsDNA repair templates with homology to the DNA flanking the...base editors convert adenine to inosine, which is replaced by guanosine to create A->G (or T->C on the opposite...nicks create a double-strand break (DSB) that is repaired using error-prone non-homologous end joining (...

  20. Cre-lox system

    Type
    Collection
    ...and high-titer viral preparations of plasmids that are currently in our repository. ID Plasmid Description...sites AAV Uchida Cre Reporters and Tools In this subtype of loxP plasmids, reporter genes indicate which...Mammalian Pelczar 62732 Cre Reporter DsRED and EGFP Cre recombinase reporter Lentiviral Geijsen 65726 pLV-CMV-LoxP-DsRed-LoxP-eGFP...Vectors Cre-containing Plasmids loxP Constructs Cre Reporters & Tools Additional Resources References The Cre-lox...Fluorescent Cre: The fusion of Cre to a fluorescent reporter enables visualization of Cre expression. Optimized... of choice. We also offer ready-to-use viral preparations of select Cre expression plasmids. Addgene's...HRE:GFP-pGK:Cre Cre and hypoxia-responsive GFP reporter HRE Lentiviral Jacks 69570 pAAV-EF1a-N-CretrcintG...
Showing: 21 - 40 of 123 results