...Monomer? Addgene's plasmid repository contains a variety of fluorescent protein plasmids. Use this guide...comprehensive collection consisting of ORFs tagged with a variety of fluorescent proteins, as well as over 100 empty...Community Resources FPbase from Talley Lambert at Harvard Medical School Fluorescent Biosensor Database designed...

...since then scientists have engineered numerous GFP-variants and non-GFP proteins that result in a diverse ...fluorescent proteins created by Talley Lambert at Harvard Medical School More References below Jump to: Blue...Thorn Lab Vectors - Includes tagging with a wide variety of colors, some specifically optimized for yeast...FPbase : A database created by Talley Lambert at Harvard Medical School Zeiss Microscopy : Optical Highlighters...

...Addgene Organizing Committee: Dr. Constance Cepko, Harvard Medical School Dr. Melina Fan, Dr. Susanna Bachle...Rego, Addgene Confirmed Speakers Constance Cepko Harvard Medical School Ali Cetin Allen Institute for Brain...e.g., publication's permission). Connie Cepko, Harvard Medical School Ali Cetin and Shenqin Yao, Allen...

... a large number of zinc fingers that recognize various nucleotide triplets. With some trial and error,...from each other by two amino acids, their repeat-variable di-residue (RVD). Ultimately, it is this RVD that...31 repeats can be assembled and inserted into a variety of backbones in just a few steps ( Figure 2 ). ...

...gRNA sequence exactly match your genomic target? Variation between a given gRNA sequence and your genomic...gRNA activity. Know your target. Which species or variant of Cas9 ( S. pyogenes, S. aureus etc.) was this...compatible with a single species or PAM binding variant of Cas9 (e.g. Wild-type SpCas9 must be used with...elegans TCCGCTACCATAGGCACCA 71479 cut VQR Cas9 variant 26680661 Fire dpy‐10 C. elegans GCTACCATAGGCACCACGAG...

... to find plasmids that can be used to engineer various types of viruses. Lentivirus Plasmids | Guide Lentiviruses... immunogenicity. Different types of viruses also vary in the composition of their viral genome, with some...NIH provides basic guidelines for working with various types of viruses; however, these guidelines may...

...independent gRNA plasmids can be paired with a wide variety of plasmids and therefore are not limited to a ...denoted as such. Want more information on the wide variety of Cas enzymes? CasPEDIA is an encyclopedia of ...plasmids for assembly of single gRNA vectors with various promoters, plasmids for assembling multiple gRNAs...into one vector, and plasmids that provide Cas9 variants. All components are then assembled into a single...

...nervous system function. While there is wide variability in symptoms even within patients with the same...repository Tissue Banks (Link opens in a new window) Harvard Brain Bank (Link opens in a new window) Autism ...algorithms for data analysis to calculate heart rate variability (Link opens in a new window) Protocols.io - protocol...

...ChETA, C1V1 (t/t) Inhibitory Arch and variants eNpHR and variants Jaws stGtACR iC++ OPN3 PdCO PPO Bidirectional...AAVrg PHPeB Clear Filters ID Name Promoter Opsin Variant Tag Activity Serotype PI 20071 pACAGW-ChR2-Venus-AAV...

...including calcium sensors, under the control of various promoters. Sensor Green Calcium Sensors GCaMP8 ...Calcium Sensor: GCaMP7c 105321 pGP-AAV-syn-jGCaMP7c variant 1513-WPRE Syn jGCaMP7c none Constitutive 1 Kim ...Kim 105322 pGP-AAV-syn-FLEX-jGCaMP7c variant 1513-WPRE Syn jGCaMP7c none Cre 1 Kim Calcium Sensor: GCaMP6f...

... your protein of interest resides. There are a variety of methods for determining subcellular localization...fluorescent fusion proteins that are targeted to various organelles or subcellular structures. You should...proteins. General Structures The cell contains a variety of organelles and subcellular structures each with...

...to generate and characterize single nucleotide variant lines in an isogenic cellular background. All cell...create cell lines with endogenously-tagged gene variants or to differentiate human iPSCs into other cell...

... activating or upregulating gene expression in various model organisms....specific locus. Want more information on the wide variety of Cas enzymes? CasPEDIA is an encyclopedia of ...

...for AAV production or direct transfection, slow variant Kaspar Podgorski 135421-AAV1 pAAV-syn-FLEX-axon-jYCaMP1s...for AAV production or direct transfection, slow variant Kaspar Podgorski 135424-AAV1 pAAV-syn-jYCaMP1s ...for AAV production or direct transfection, slow variant Kaspar Podgorski 135630-AAV1 pAAV-S5E2-dTom-nlsdTom...

... with EF1alpha addition of an mCherry reporter variant nuclear reporters via an H2B fusion shuttle plasmid...fusion for gene activation CRISPR/Cas9 Plasmid Variants Figure 1: Schematic of the CRISPR Collection. ...

...Find plasmids to overexpress various fluorescent markers and tag your proteins of interest with fluorescent...codon-optimized fluorescent proteins (FPs) in a variety of plasmids allows for constitutive overexpression...

... community demonstrating AAV performance under various experimental conditions. Protocols Written and ...and scientists across the research community on various aspects of viral vectors, including troubleshooting...

...Addgene's CRISPR collections feature plasmids for a variety of organisms, including mammals, plants, bacteria...an empty vector for your experiment based on a variety of factors. gRNAs gRNA plasmids to target specific...

...immortalized cells (see Weinberg Lab page for resistance variants) Weinberg 1773 pBABE-hygro-hTERT MoMLV Expression...expression; also expresses GFP. Also see pMIG-w , a variant that has WRE, a post-transcriptional regulatory...

...applications. The specific QC experiments performed varies for each antibody catalog item. Whenever possible...primers targeting the heavy chain and/or light chain variable regions. The PCR product is purified, sequenced...