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Addgene

Enter a sequence to perform a BLAST-based search of Addgene plasmid sequence databases.

In Category

We narrowed to 71 results for: Vit

Showing: 21 - 40 of 71 results

  1. Antibody Guide

    Type
    Collection
    ...(~25 kD), but tend to have lower affinity and longevity. Single-domain antibodies - Single-domain antibodies...monoclonal antibodies tend to have less cross-reactivity and give lower background signals than polyclonal...interest is knocked down or out, and antibody reactivity is compared between the wild-type and the knockdown...cell line with low or no expression; antibody reactivity is then compared between the knock-in cell line...

  2. CRISPR References and Information

    Type
    Collection
    ...CRISPRko/a/i sgRNA sequences to maximize on-target activity for selected target(s). Uses human, mouse, and...CRISPR systems with wiki entries describing enzyme activity, experimental considerations, and more. CasPEDIA...107 KB Chen and Wente Zebrafish: gRNA cloning, in vitro transcription, injection gRNA core ; Cas9 ; optimized...

  3. Optogenetics AAV Preps

    Type
    Collection
    ... Optogenetic tools can be used to control cell activity in living tissue with light. Optogenetics is typically...fluorescent protein Blue-wavelength fluorescent protein Activity Cre dependent Flp dependent Cre and Flp dependent...Clear Filters ID Name Promoter Opsin Variant Tag Activity Serotype PI 20071 pACAGW-ChR2-Venus-AAV CAG ChR2...

  4. Luciferase Plasmid Collection

    Type
    Collection
    ... the emission of photons. Its robust signal, sensitivity, wide dynamic range, and resistance to photobleaching...screening of transformants in cloning process. Lynne Postovit 64034 pGL4Luc-RLuc Firefly, Renilla Dual reporter...G-CASE : BRET-based sensors for reporting on GPCR activity. AIMTOR : BRET biosensor for live imaging of mTOR...

  5. CRISPR History and Development for Genome Engineering

    Type
    Collection
    ...could be programmed for targeted DNA cleavage in vitro. In 2013, Cong et al. and Mali et al. described ...gRNAs: Truncated gRNAs display less off-target activity. High fidelity Cas9s: Three high fidelity Cas9...eSpCas9, and HypaCas9 display very low off-target activity due to rationally designed mutations. Researchers...

  6. CRISPR Plasmids - RNA Targeting

    Type
    Collection
    ...RNAs regardless of homology to the crRNA. This activity provides a stark contrast to Cas9 and Cpf1, which...CRISPR systems with wiki entries describing enzyme activity, experimental considerations, and more, created...

  7. Fluorescent Protein Guide

    Type
    Collection
    ...Regulate Biological Activity Use fluorescent proteins to modulate biological activity, like transcription...

  8. CRISPR Plasmids - Zebrafish

    Type
    Collection
    ...BsmBI In vitro transcription none, need Cas9 plasmid Chen and Wente 42250 DR274 T7 BsaI In vitro transcription...

  9. SARS-CoV-2 Pseudotyped Virus

    Type
    Collection
    ... that can be used for measuring infectivity and neutralizing activity are listed below: pBOBI-FLuc - Lentiviral...

  10. Zinc Finger Consortium: Zinc Finger Arrays

    Type
    Collection
    ... arrays listed show transcriptional activation activity of three-fold or greater in a well-characterized...reporter system but have NOT yet been tested for activity as ZFNs in zebrafish. Target Gene Name Zinc Finger...

  11. Distribution to Industry

    Type
    Collection
    ...Mikel Garcia-Marcos 10 plasmids for detecting GPCR activity in a variety of cell types. Protein Purification...partner labs. Learn More ID Recombinant Antibody Reactivity Recommended Applications PI Don't See What You're...

  12. p53 Pathway

    Type
    Collection
    ...regulators, increase its stability and DNA binding activity, and allow it to bind transcriptional co-activators... causes a large reduction in tumor suppression activity, leading to the development of tumors in early...

  13. Lentiviral Prep Service

    Type
    Collection
    ... ID Name Insert Function Antibiotic Resistance Activity PI 52962 lentiCas9-Blast Sp Cas9 Cut Blasticidin...activator . ID Name Insert Antibiotic Resistance Activity PI 61422 dCAS9-VP64_GFP dCAS9 (D10A, H840A) none...

  14. Arf GTPase Family

    Type
    Collection
    ...lipid droplets, the nucleus, and mitochondria. The activity of GTPases is regulated both upstream (by GEFs...with each component involved in GTPase signaling activity. Figure 1: Locations of ARF superfamily members...

  15. Cre-Lox and Other Site-Specific Recombinases

    Type
    Collection
    ...enabling precise genetic manipulation without cross-reactivity with other recombinase systems. Enzyme Target...Teaching an Old DOG New Tricks: Controlling Protein Activity with GFP Plasmids 101: FLEx Vectors Optimizing...

  16. Retrograde AAV viral preps

    Type
    Collection
    ...with PCR, purity assessment by silver staining, in vitro and (when possible) in vivo testing, and full sequencing...Molecular Tools Clear Filters ID Name Promoter Activity Category PI 37825 AAV-CAG-GFP CAG GFP Control ...

  17. Biosensor AAV Preps

    Type
    Collection
    ...EF1a GFAP/GfaABC1D Synapsin E2 regulatory element Activity Cre-dependent Flp-dependent Cre and Flp-dependent...PHPeB Clear Filters ID Name Promoter Sensor Label Activity Serotype PI Acetylcholine Sensors 137950 pAAV....

  18. Genetic Code Expansion

    Type
    Collection
    ...acid can also be used to intentionally change the activity of a protein (e.g. converting a DNA binding protein...protein to a DNA cleaving enzyme) or to regulate the activity of a protein so that it is responsive to specific...

  19. Immunology Research Plasmids and Resources

    Type
    Collection
    ...containing MGC138371, U2B, URP VDR vitamin D (1,25- dihydroxyvitamin D3) receptor NR1I1 VEGFA vascular ...C-X-C motif) ligand 1 (melanoma growth stimulating activity, alpha) FSP, GRO1, GROa, MGSA, MGSA-a, NAP-3, ...AUTS9, HGFR, RCCP2, c-Met MIA melanoma inhibitory activity CD-RAP MIF macrophage migration inhibitory factor...

  20. Neurodegeneration Plasmid Collection

    Type
    Collection
    ...-4T2 Parkin RBR PRKN GST tac Alzheimer's Rachel Klevit 31606 pHA-deltaN335-Kin1A KIF5A HA CMV ALS Anthony...ALS Eugene Yeo 156883 pD649-HAsp-CSF1R-Fc(DAPA)-AviTag-6xHis CSF1R Fc(DAPA), Avi, His CMV, SP6 Hereditary...leukoencephalopathy Chris Garcia 156931 pD649-HAsp-ERBB4-Fc(DAPA)-AviTag-6xHis ERBB4 Fc(DAPA), Avi, His CMV, SP6 ALS Chris...Chris Garcia 157115 pD649-HAsp-TREM2-COMP5AP-AviTag-9xHis TREM2 COMP5AP, Avi, His CMV, SP6 Alzheimer's ...Alzheimer's Chris Garcia 157447 pD649-HAsp-CSF1R-COMP5AP-AviTag-9xHis CSF1R COMP5AP, Avi, His CMV, SP6 Hereditary...leukoencephalopathy Chris Garcia 157495 pD649-HAsp-ERBB4-COMP5AP-AviTag-9xHis ERBB4 COMP5AP, Avi, His CMV, SP6 ALS Chris...
Showing: 21 - 40 of 71 results