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We narrowed to 31 results for: cas9 expression plasmid

Showing: 21 - 31 of 31 results
  1. CRISPR Plasmids - Purify Genomic Loci

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    Collection
    ...Bacteria ID Plasmid Gene/Insert Promoter Selectable Marker PI Publication Return to top Yeast ID Plasmid Gene...selection of CRISPR plasmids and resources. Find more CRISPR functions along with plasmids categorized by ...suggestions for other plasmids that should be added to this list? Fill out our Suggest a Plasmid form or e-mail... for enChIP, as well as an anti-Cas9 antibody. Biotin tagging of dCas9 can be achieved by fusing a biotin...biotin acceptor site to dCas9 and co-expressing BirA biotin ligase, as seen in the CAPTURE system . The...Catalytically inactive CRISPR plasmids for purification of target loci. CRISPR...Browse, sort, or search the tables below for CRISPR plasmids designed for the purification of genomic loci....
  2. Botman-Teusink Yeast FP Collection

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    Collection
    ...TCGATGAATTCGAGCTCG–3' ID Plasmid Selectable Marker Tags Publication FP Overexpression Plasmids for constitutive...Yeast Localization and Overexpression (MyLO) CRISPR-Cas9 Toolkit Other Addgene Plasmids Bas Teusink Lab Materials...Find plasmids to overexpress various fluorescent markers and tag your proteins of interest with fluorescent...proteins (FPs) in a variety of plasmids allows for constitutive overexpression of FPs and tagging of genes...constitutive overexpression of codon-optimized FPs in yeast. Vectors are based on the pDRF1 plasmid (Loqué et al...proteins. These vectors are based on the pFA6a-link plasmid (Sheff et al., 2004; Lee et al., 2013), and are...the PMA1 promoter and the URA3 marker, the p415 plasmid (Loqué et al., 2007) containing the TEF1 promoter...
  3. Distribution to Industry

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    Collection
    ...collection of plasmids that are available to industry. Learn more about the available plasmids and how to...Distribution to Industry Featured Collections Search Plasmids Plasmids Kits Pooled Libraries Antibodies Ordering ...additional filters such as expression, vector type, species, and more. Plasmids Browse the table below to...see all of the plasmids that are currently available to industry scientists. ID Plasmid Gene/Insert PI...assembly. MoClo Toolkit Plant Expression Sylvestre Marillonnet 95 plasmids for cloning and assembling standardized...Biolabs Cancer Research from Sinai Health System Plasmids from the Structural Genomics Consortium Solute...Foundation Search Use Addgene's Search to find plasmids available to industry scientists while also adding...
  4. Cre-Lox and Other Site-Specific Recombinases

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    ...Site-Specific Recombinases Cre Plasmids Flp Plasmids Dre Plasmids Other Recombinase Plasmids Recombinase-Dependent...genome or control gene expression or skip ahead to browse highlighted plasmids expressing Cre , Flp , Dre ,...vectors. Highlighted Plasmids Browse the tables below to find popular plasmids expressing your favorite site-specific...full catalog for plasmids expressing Cre recombinase and filter the results by expression system and vector... with GFP Plasmids 101: FLEx Vectors Optimizing AAV DIO and FLEx Vector Expression Plasmids 101: Knockout...full catalog for plasmids expressing Flp recombinase and filter the results by expression system and vector...full catalog for plasmids expressing Dre recombinase and filter the results by expression system and vector...
  5. Rett Syndrome

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    Collection
    ... Jaenisch labs Plasmids MECP2 Plasmids The table below highlights plasmids that express or target the ...CRISPR resources for a full selection of plasmids expressing Cas9 and empty gRNA backbones. A few examples...approximately half of cells expressing wild-type MECP2 and half expressing mutant MECP2 . Skewing of these...information on generating iPSCs using plasmids, please see Addgene's Plasmids for Stem Cell Research page. CRISPR... search bar or sort buttons to find plasmids based on: Expression system (mammalian or bacterial) Insert...Broswe plasmids and other research tools for use in Rett Syndrome research. Plasmid... Plasmid Collections Rett ...
  6. Tetracycline Inducible Expression

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    Collection
    ...dox-regulated plasmids . ID Plasmid Description PI 63800 PB-TRE-dCas9-VPR Tet-inducible expression of SP-dCas9...Lentiviral Tet-On plasmid for inducible expression of shRNA with puromycin selection. See Plasmid #21916 for ...Resources Mammalian shRNA Plasmids Cre-lox Plasmids Stem Cell Research Plasmids Blog: Inducible Promoters...tet-inducible Cas9-2A-EGFP expression. Based on LentiCRISPR v2 . Adam Karpf 167935 pLenti-tetON-KRAB-dCas9-DHFR-EF1a-TagRFP...-2A-tet3G Tet-inducible expression of KRAB-dCas9-DHFR for CRISPRi. See Plasmid #167937 for CRISPRa Emma...gene expression and find plasmids for your experiments using tet- or dox-inducible systems. Plasmid...Tetracycline (Tet) Inducible Expression Tetracycline (Tet) Inducible Expression Tetracycline Off Tetracycline...
  7. Bacterial Expression Systems

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    ...selection of plasmids and tags for protein purification. The pTD Plasmid Series contains plasmids suitable...and expression species. The New England Biolabs Cell-Imaging Plasmid Collection contains plasmids for ...turn on expression only at a specific time. This collection contains plasmids whose expression levels ...bacterial systems. ID Plasmid Promoter Inducer Expression Species PI 44249 pdCas9-bacteria pTetO Anhydrotetracycline...Find bacterial expression plasmids that can be used for protein purification, protein visualization, ...controlled gene expression, reporter plasmids and genome engineering of bacteria. Plasmid...Bacterial Expression Systems Bacterial Expression Systems Protein Purification Protein Visualization Protein...
  8. Bikard Lab - CRISPR Repression Collection

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    ...From the Bikard Lab, CRISPR plasmid collection for modulating expression levels of two targets independently... can obtain a range of expression levels. This collection comprises 20 plasmids, each carrying two CRISPR...nucleotides. Together, these plasmids allow to explore the whole range of expression levels for these two reporters... modulate expression of two genes without cross‐talk. (A) Schematic of the strain expressing two reporters... the expression levels of two targets independently. The RNA-programmed DNA-binding protein dCas9 is expressed... expressed along a CRISPR array that contains 20 bp "addresses", where dCas9 is going to bind on the chromosome...enzymes. The pLC97 plasmid allows for quick integration of the aTc-inducible pTet-dCas9 in the attachment...
  9. CRISPR Plasmids - Activate Gene Expression

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    ...also express a gRNA, you will need to use a separate gRNA expression plasmid to target the dCas9-activator...CRISPR plasmids for activating or upregulating gene expression in various model organisms. CRISPR...Bacteria ID Plasmid Gene/Insert Promoter PI Publication Return to top Drosophila ID Plasmid Gene/Insert...C. elegans ID Plasmid Gene/Insert Promoter PI Publication Return to top Yeast ID Plasmid Gene/Insert Promoter...selection of CRISPR plasmids and resources. Find more CRISPR functions along with plasmids categorized by ...suggestions for other plasmids that should be added to this list? Fill out our Suggest a Plasmid form or e-mail...direct the dCas9-activator to promoter or regulatory regions of your gene of interest. If the plasmid that ...
  10. CRISPR Plasmids and Resources

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    Collection
    ...select CRISPR lentiviral plasmids. CRISPR Plasmid Kits : Find groups of plasmids that are used together ...resources or browse plasmids below by function. Addgene's CRISPR collections feature plasmids for a variety ...variety of factors. Browse Plasmids by Model Organism Bacteria CRISPR plasmids designed for use in bacteria.... elegans CRISPR plasmids designed for use in C. elegans . Drosophila CRISPR plasmids designed for use...Mammalian CRISPR plasmids designed for use in mammalian systems. Parasites CRISPR plasmids designed for use...parasites. Plant CRISPR plasmids designed for use in plants. Yeast CRISPR plasmids designed for use in yeast...yeast. Xenopus CRISPR plasmids designed for use in Xenopus . Zebrafish CRISPR plasmids designed for use in...
  11. CRISPR Plasmids - Bacteria

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    ...following CRISPR plasmids have been designed for use in bacteria. To find emtpy gRNA plasmids, visit Addgene's...Empty gRNA Expression Vectors page and search the table for "Bacteria". Activate ID Plasmid Gene/Insert... to top Base Edit ID Plasmid Promoter PI Publication Return to top Cut ID Plasmid Gene/Insert Promoter... Interfere ID Plasmid Gene/Insert Promoter PI Publication Return to top Nick ID Plasmid Gene/Insert Promoter...Prime Edit ID Plasmid Gene/Insert Promoter PI Publication Return to top Purify ID Plasmid Gene/Insert Promoter...selection of CRISPR plasmids and resources. Find more CRISPR functions along with plasmids categorized by ...suggestions for other plasmids that should be added to this list? Fill out our Suggest a Plasmid form or e-mail...
Showing: 21 - 31 of 31 results