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We narrowed to 45 results for: cas9 expression plasmid

Showing: 21 - 40 of 45 results

  1. Brzezinski Lab CRISPR Collection

    Type
    Collection
    ...and 175574 express dCas9-KRAB-MeCP2 for repression. Plasmids 175571 and 175572 express dCas9-VPR for activation...shuttle plasmid for shuttling U6-guide cassettes to make dual guide expressing plasmids dCas9-KRAB-MeCP2...guides can be expressed from the same plasmid by utilizing one of the dual guide shuttle plasmids to insert...enhancer sequence repression dCas9-VPR fusion for gene activation CRISPR/Cas9 Plasmid Variants Figure 1: Schematic...advantage of the Zhang lab all-in-one (guide/Cas9/reporter) pX458 plasmid family. They have made several modifications... the CRISPR Collection. Plasmids 159654 and 159655 are single guide expressing double stranded break constructs...constructs. Plasmids 171098 and 175570 allow conversion to a dual guide construct. Plasmids 175573 and ...

  2. AAV Molecular Tools

    Type
    Collection
    ...encode Cas9 nuclease used in the CRISPR-Cas9 system for targeted genome editing. ID Name Expression System...AAV-CMV::NLS-SaCas9-NLS-3xHA-bGHpA CMV-driven, constitutive Expression of human codon-optimized Cas9 from Staphylococcus...with tetracycline (tet)-inducible expression systems . ID Name Expression System Activity Serotype PI 99118...Cre-dependent expression of ER-localized TurboID (with HA and V5 tag). 8 Long Overexpression Tools These...These AAV encode various genes for overexpression. ID Name Expression System Activity Serotype PI 58376 pAAV...99118 pAAV-CAG-tTA CAG-driven, constitutive Expression of the tet-off transactivator (tTA) 2 Gradinaru ...pAAV-ihSyn1-tTA Inducible Synapsin promoter (ihSyn) Expression of the tet-off transactivator (tTA) with a positive...

  3. CRISPR Plasmids - Plants

    Type
    Collection
    ...of Cas-containing plasmids. ID Plasmid Promoter Cloning Enzyme Co-expressed Cas9 Cas9 System Selection ...also express a gRNA, you will need to use a separate gRNA expression plasmid to target the dCas9-activator...separate gRNA expression plasmid to target the dCas9-repressor to your specific locus. ID Plasmid Gene/Insert...PI Publication Empty gRNA Expression Vectors Select a gRNA expression plasmid based on factors such as ...CRISPR expression plasmids that have been designed for use in plants. CRISPR...repair (HDR). ID Plasmid Gene/Insert Promoter Selectable Marker PI Publication Prime Edit Cas9 H840A nickase...of the coding sequence. If the plasmid you’re using does not also express a gRNA, you will need to use ...

  4. CRISPR History and Development for Genome Engineering

    Type
    Collection
    ...High fidelity Cas9s: Three high fidelity Cas9 variants , SpCas9-HF, eSpCas9, and HypaCas9 display very ...III and Cas9 enzymes, which together separate the individual crRNAs. (3) The tracrRNA and Cas9 nuclease...posts , or find CRISPR plasmids for your research. Addgene has CRISPR plasmids for the following models...Staphylococcus aureus (SaCas9) is smaller than SpCas9 and more easily packaged in AAV. Cas9 orthologs also have...beyond SpCas9, moving us closer to being able to target every locus in every genome. Multiple Cas9 orthologs...capability of Cas9 to make other types of modifications at specific loci. Activate or Repress : dCas9 fused to...known as CRISPR arrays. Separate Cas proteins then express and process the CRISPR loci to generate CRISPR ...

  5. CRISPR Plasmids - dCas9-FokI

    Type
    Collection
    ...tables below for CRISPR-FokI plasmids. Plasmids are available for expression in mammalian systems and Drosophila...Drosophila. Mammalian ID Plasmid Gene/Insert Promoter PI Publication Drosophila ID Plasmid Gene/Insert Promoter...Yeast ID Plasmid Gene/Insert Promoter PI Publication Do you have suggestions for other plasmids that should...CRISPR plasmids with dCas9 fused to FokI for creating double-strand breaks (DSBs). CRISPR... CRISPR: dCas9-FokI Browse CRISPR Plasmids By Function Genome Editing Cut Base Edit Nick Prime Edit Transcriptional...Tools CRISPR Blog Posts A catalytically inactive Cas9 (dCas9) is fused to FokI nuclease. When FokI dimerizes... be added to this list? Fill out our Suggest a Plasmid form or e-mail [email protected] to help us improve...

  6. CRISPR Plasmids - Cascade-Cas3

    Type
    Collection
    ...below for CRISPR Cascade-Cas3 plasmids. Plasmids are available for expression in mammalian systems, bacteria...the Cascade complex to express equally. To combat this, some CRISPR-Cas3 plasmids take an all-in-one approach...plants. Mammalian ID Plasmid Gene/Insert Promoter PI Publication Bacteria ID Plasmid Gene/Insert Promoter...suggestions for other plasmids that should be added to this list? Fill out our Suggest a Plasmid form or e-mail...Find plasmids for CRISPR-Cas and Cascade for large genomic deletions. CRISPR... CRISPR Cas3 CRISPR Plasmids: Cascade-Cas3...Cascade-Cas3 Browse CRISPR Plasmids By Function Genome Editing Cut Base Edit Nick Prime Edit Transcriptional...

  7. CRISPR Plasmids - gRNAs

    Type
    Collection
    ... any of these gRNA plasmids and review the publication associated with each plasmid for more information...gRNA Plasmids Please note that the following table lists both published and unpublished plasmids containing...information on the experimental use of these plasmids. ID Plasmid Gene/Insert Vector Type PI Publication Do...suggestions for other plasmids that should be added to this list? Fill out our Suggest a Plasmid form or e-mail...species or PAM binding variant of Cas9. For instance, wild-type SpCas9 must be used with targets that are...gRNA expression vectors available from Addgene. CRISPR... CRISPR gRNAs CRISPR Plasmids: gRNAs Browse CRISPR Plasmids...

  8. CRISPR Plasmids - Purify Genomic Loci

    Type
    Collection
    ...below for CRISPR plasmids to help purify genomic loci. Plasmids are available for expression in mammalian ...Mammalian ID Plasmid Gene/Insert Promoter Selectable Marker PI Publication Bacteria ID Plasmid Gene/Insert...suggestions for other plasmids that should be added to this list? Fill out our Suggest a Plasmid form or e-mail... for enChIP, as well as an anti-Cas9 antibody. Biotin tagging of dCas9 can be achieved by fusing a biotin...biotin acceptor site to dCas9 and co-expressing BirA biotin ligase, as seen in the CAPTURE system. The ...Catalytically inactive CRISPR plasmids for purification of target loci. CRISPR...Purify Genomic Loci Browse CRISPR Plasmids By Function Genome Editing Cut Base Edit Nick Prime Edit Transcriptional...

  9. Neurodegeneration Research Collection

    Type
    Collection
    ...Tools Neurodegeneration Plasmid Collection A collection of plasmids expressing wild type or mutant ORFs... and plasmid collections that may help with your neurodegeneration research. Disease Info Plasmid Collection...linked to neurodegenerative disorders. Find plasmids expressing HTT, C9ORF72, LRRK2, Synuclein, and more....bioRxiv. 2022 Dec 2. Use PiggyBac plasmids with tet-inducible expression of transcription factors for sensory...Subcellular Localization, Optogenetics Plasmids, and Chemogenetics Plasmids. New and Noteworthy: Explore NeuroPAL...more. Plasmids provided by the foundation can be found on our Michael J. Fox Foundation Plasmid Resource...Find out about the plasmid collections available at Addgene that may be useful for neurodegeneration ...

  10. Empty Backbones - Choosing Your Perfect Plasmid Backbone

    Type
    Collection
    ...Collection NLS Nuclear localization dCas9 plasmid - dCas9 expression plasmid without effector fusions; 3X Flag...Species-Specific Expression If you want to drive expression of your favorite gene, you will need a plasmid with...EF1a, CAG, Ubc Transient expression - Gradia Lab plasmids for mammalian expression, Ligation Independent ...Empty mammalian expression plasmid for cloning untagged proteins pcDNA3.1-mCherry - Expresses mCherry tag ...TEV cleavage Transient expression - Gradia Lab plasmids for bacterial expression, Ligation Independent ... Expression - Rubin lab plasmids for Drosophila expression pMT-puro - Inducible...C-terminal GFP for mammalian expression pLV-mCherry - 3rd gen lentiviral plasmid expressing mCherry pBad-EBFP2 ...

  11. CRISPR Plasmids - RNA Targeting

    Type
    Collection
    ...below for CRISPR RNA targeting plasmids. Plasmids are available for expression in mammalian systems, bacteria...CRISPR RNA Targeting CRISPR Plasmids: RNA Targeting Browse CRISPR Plasmids By Function Genome Editing Cut ...Mammalian ID Plasmid Gene/Insert Promoter Selectable Marker PI Publication Bacterial ID Plasmid Gene/Insert...Publication Plant ID Plasmid Gene/Insert PI Publication Do you have suggestions for other plasmids that should ...CRISPR plasmids for targeting RNA sequences, which include the enzymes C2c2/Cas13a and Cas13b. CRISPR...crRNA. This activity provides a stark contrast to Cas9 and Cpf1, which require that each DNA target have... be added to this list? Fill out our Suggest a Plasmid form or e-mail [email protected] to help us improve...

  12. CRISPR Plasmids - Parasites

    Type
    Collection
    ...CRISPR expression plasmids that have been designed for use in parasites....suggestions for other plasmids that should be added to this list? Fill out our Suggest a Plasmid form or e-mail...Plasmids: Parasites Browse CRISPR Plasmids By Function Genome Editing Cut Base Edit Nick Prime Edit Transcriptional...research for decades. Recent developments with CRISPR-Cas9 gene editing have propelled both reverse and forward...development. This collection brings together some of the plasmids that have been used in these efforts. The content...occurs at a much lower efficiency than NHEJ. ID Plasmid Gene/Insert Selectable Marker PI Publication Libraries...Editing Other Applications Purify Tag Visualize dCas9-FokI Screen Pooled Libraries gRNAs Empty gRNA Vectors...

  13. Botman-Teusink Yeast FP Collection

    Type
    Collection
    ...TCGATGAATTCGAGCTCG–3' ID Plasmid Selectable Marker Tags Publication FP Overexpression Plasmids for constitutive...Yeast Localization and Overexpression (MyLO) CRISPR-Cas9 Toolkit Other Addgene Plasmids Bas Teusink Lab Materials...Find plasmids to overexpress various fluorescent markers and tag your proteins of interest with fluorescent...proteins (FPs) in a variety of plasmids allows for constitutive overexpression of FPs and tagging of genes...constitutive overexpression of codon-optimized FPs in yeast. Vectors are based on the pDRF1 plasmid (Loqué et al...proteins. These vectors are based on the pFA6a-link plasmid (Sheff et al., 2004; Lee et al., 2013), and are...the PMA1 promoter and the URA3 marker, the p415 plasmid (Loqué et al., 2007) containing the TEF1 promoter...

  14. Distribution to Industry

    Type
    Collection
    ...collection of plasmids that are available to industry. Learn more about the available plasmids and how to...Distribution to Industry Featured Collections Search Plasmids Plasmids Kits Pooled Libraries Antibodies Ordering ...additional filters such as expression, vector type, species, and more. Plasmids Browse the table below to...see all of the plasmids that are currently available to industry scientists. ID Plasmid Gene/Insert PI...assembly. MoClo Toolkit Plant Expression Sylvestre Marillonnet 95 plasmids for cloning and assembling standardized...Biolabs Cancer Research from Sinai Health System Plasmids from the Structural Genomics Consortium Solute...Foundation Search Use Addgene's Search to find plasmids available to industry scientists while also adding...

  15. Cre-Lox and Other Site-Specific Recombinases

    Type
    Collection
    ...Site-Specific Recombinases Cre Plasmids Dre Plasmids Flp Plasmids Other Recombinase Plasmids Recombinase-Dependent...genome or control gene expression or skip ahead to browse highlighted plasmids expressing Cre , Flp , Dre ,...vectors. Highlighted Plasmids Browse the tables below to find popular plasmids expressing your favorite site-specific...full catalog for plasmids expressing Cre recombinase and filter the results by expression system and vector... with GFP Plasmids 101: FLEx Vectors Optimizing AAV DIO and FLEx Vector Expression Plasmids 101: Knockout...full catalog for plasmids expressing Flp recombinase and filter the results by expression system and vector...full catalog for plasmids expressing Dre recombinase and filter the results by expression system and vector...

  16. Rett Syndrome

    Type
    Collection
    ... Jaenisch labs Plasmids MECP2 Plasmids The table below highlights plasmids that express or target the ...CRISPR resources for a full selection of plasmids expressing Cas9 and empty gRNA backbones. A few examples...approximately half of cells expressing wild-type MECP2 and half expressing mutant MECP2 . Skewing of these...information on generating iPSCs using plasmids, please see Addgene's Plasmids for Stem Cell Research page. CRISPR... search bar or sort buttons to find plasmids based on: Expression system (mammalian or bacterial) Insert...Broswe plasmids and other research tools for use in Rett Syndrome research. Plasmid... Plasmid Collections Rett ...

  17. Tetracycline Inducible Expression

    Type
    Collection
    ...dox-regulated plasmids . ID Plasmid Description PI 63800 PB-TRE-dCas9-VPR Tet-inducible expression of SP-dCas9...Mammalian shRNA Plasmids Collection Cre-lox Plasmids Collection Stem Cell Research Plasmids Collection Tetracycline...Lentiviral Tet-On plasmid for inducible expression of shRNA with puromycin selection. See Plasmid #21916 for ...tet-inducible Cas9-2A-EGFP expression. Based on LentiCRISPR v2 . Adam Karpf 167935 pLenti-tetON-KRAB-dCas9-DHFR-EF1a-TagRFP...-2A-tet3G Tet-inducible expression of KRAB-dCas9-DHFR for CRISPRi. See Plasmid #167937 for CRISPRa Emma...gene expression and find plasmids for your experiments using tet- or dox-inducible systems. Plasmid...Tetracycline (Tet) Inducible Expression Tetracycline (Tet) Inducible Expression You may also like... Blog...

  18. Bacterial Expression Systems

    Type
    Collection
    ...selection of plasmids and tags for protein purification. The pTD Plasmid Series contains plasmids suitable...and expression species. The New England Biolabs Cell-Imaging Plasmid Collection contains plasmids for ...turn on expression only at a specific time. This collection contains plasmids whose expression levels ...bacterial systems. ID Plasmid Promoter Inducer Expression Species PI 44249 pdCas9-bacteria pTetO Anhydrotetracycline...Find bacterial expression plasmids that can be used for protein purification, protein visualization, ...controlled gene expression, reporter plasmids and genome engineering of bacteria. Plasmid...Bacterial Expression Systems Bacterial Expression Systems Protein Purification Protein Visualization Protein...

  19. Bikard Lab - CRISPR Repression Collection

    Type
    Collection
    ...From the Bikard Lab, CRISPR plasmid collection for modulating expression levels of two targets independently... can obtain a range of expression levels. This collection comprises 20 plasmids, each carrying two CRISPR...nucleotides. Together, these plasmids allow to explore the whole range of expression levels for these two reporters... modulate expression of two genes without cross‐talk. (A) Schematic of the strain expressing two reporters... the expression levels of two targets independently. The RNA-programmed DNA-binding protein dCas9 is expressed... expressed along a CRISPR array that contains 20 bp "addresses", where dCas9 is going to bind on the chromosome...enzymes. The pLC97 plasmid allows for quick integration of the aTc-inducible pTet-dCas9 in the attachment...

  20. CRISPR Plasmids - Repress Gene Expression

    Type
    Collection
    ... for CRISPR plasmids for transcriptional inhibition. Plasmids are available for expression in mammalian...use a separate gRNA expression plasmid to target the dCas9-repressor to your specific locus. Want more information...of the coding sequence. If the plasmid you’re using does not also express a gRNA, you will need to use ...Mammalian ID Plasmid Gene/Insert Promoter Selectable Marker PI Publication Bacteria ID Plasmid Gene/Insert...Publication Plant ID Plasmid Gene/Insert Promoter Selectable Marker PI Publication Yeast ID Plasmid Gene/Insert...suggestions for other plasmids that should be added to this list? Fill out our Suggest a Plasmid form or e-mail...CRISPR plasmids for transcriptional repression of target genes. CRISPR...
Showing: 21 - 40 of 45 results