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Enter a sequence to perform a BLAST-based search of Addgene plasmid sequence databases.

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We narrowed to 101 results for: cha

Showing: 21 - 40 of 101 results

  1. Tetracycline Inducible Expression

    Type
    Collection
    ...Tet-regulated expression systems. Left: natural TetR mechanism; center: Tet-Off; right: Tet-On. TRE: Tet response...#21916 for neomycin selection. TetR H1-2O2 Dmitri Wiederschain 85966 EZ-Tet-pLKO-Puro Lentiviral Tet-On plasmid...expresses tTA from the CAG promoter. See article ( Chan et al., 2017 ) for other viral tTA plasmids. tTA...Neurodegenerative Disease Initiative (iNDI) Collection . Michael Ward 105840 pUCM-AAVS1-TO-hNGN2 Introduction of...for iPSC differentiation into cortical neurons Michael Ward 100798 pAAV-syn-FLEX-splitTVA-EGFP-tTA Helper...Rheenen 104102–104112 TetBow Series of plasmids for stochastic multicolor labeling for discriminating between...7792603 (Link opens in a new window) Heinz, N., Schambach, A., Galla, M., Maetzig, T., Baum, C., Loew, R...

  2. Allen Institute for Brain Science AAV Enhancer Collection

    Type
    Collection
    ...Brouner K, Casian B, Casper T, Chakka AB, Chakrabarty R, Chance RK, Chavan S, Departee M, Donadio N, Dotson...pAAV-AiE0475m-minBG-SYFP2-WPRE3-BGHpA AiP12320 AiE0475m SYFP2 Chandelier cells (Pvalb) Isocortex 230384* pAAV-AiE0475m-minBG-iCre...pAAV-AiE0475m-minBG-iCre(R297T)-BGHpA AiP13790 AiE0475m Cre Chandelier cells (Pvalb) Isocortex 214561 pAAV-AiE2566m-...CA, Potekhina L, Ransford S, Rette D, Rimorin C, Rocha D, Ruiz A, Sanchez REA, Sedeno-Cortes A, Sevigny...

  3. Ras Pathway

    Type
    Collection
    ... protein 35 ARHGEF2 Rho/Rac guanine nucleotide exchange factor 2 CCND CCND1 CCND2 CCND3 Cyclin D CDK CDK4...MLST8 MTOR associated protein, LST8 homolog MTOR Mechanistic target of rapamycin NF1 Neurofibromin 1 NFE2L2...Phosphatidylinositol-3,4,5-trisphosphate-dependent Rac exchange factor 2 PRKA PRKAA1 PRKAA2 PRKAB1 PRKAB2 PRKAG1... RAPGEF RAPGEF1 RAPGEF2 Rap guanine nucleotide exchange factor RAS KRAS MRAS NRAS Kirsten rat sarcoma ...protein SOS SOS1 SOS2 SOS Ras/Rho guanine nucleotide exchange factor SPRED SPRED1 SPRED2 SPRED3 Sprouty related...Tuberous sclerosis VAV1 Vav guanine nucleotide exchange factor 1 YAP1 Yes associated protein 1 Return ...

  4. Fluorescent Protein Guide

    Type
    Collection
    ...activity, like transcription. Michael Davidson FP Collection Browse the Michael Davidson comprehensive collection...protein-protein interactions or conformational changes within a protein. Biosensors Monitor small biomolecules...protein of interest resides by using a well-characterized fluorescent fusion protein. In Vivo Imaging ... and UCSF Molecular Expressions Galleries from Michael Davidson and FSU Spectra Database hosted at the...

  5. Caltech Systemic Capsids

    Type
    Collection
    ...nlsdTomato Control Fishell 213829 pAAV_BiCHATe27_dTomato_nlsdTomato BiCHATe27 dTomato/nlsdTomato Control Fishell...BiSSTe10 Activator ChR2 Fishell 213830 pAAV_BiCHATe27_ChR2_mCherry BiCHATe27 Activator ChR2 Fishell 213856 pAAV_BiVIPe4...Link opens in a new window) and was adapted from Challis et al. (2022). Text version of the AAV decision...Viviana Gradinaru and cite as follows: PHP.eB, PHP.S: Chan et al., 2017 Pubmed 28671695 (Link opens in a new...

  6. mTOR Pathway

    Type
    Collection
    ...Pathway Plasmids mTOR Gene List Resources The m echanistic or m ammalian t arget o f r apamycin (mTOR) is...mTORC2. Background mTORC1 & mTORC2 The better-characterized mTORC1 responds to various signals, including...known as STK11; serine/threonine kinase 11 mTOR Mechanistic target of rapamycin NF1 Neurofibromin 1 PRAS40...Mitogen-activated protein kinase associated protein 1 mTOR Mechanistic target of rapamycin p53 TP53; tumor protein p53... the translation machinery in cancer. Bhat M, Robichaud N, Hulea L, Sonenberg N, Pelletier J, Topisirovic...

  7. p53 Pathway

    Type
    Collection
    ... mutations have been characterized as gain-of-function, expanding the mechanisms by which mutant p53 is...transcriptional targets is ever-expanding; the best-characterized effects of p53 are in promoting cell cycle arrest...Thrombospondin 1 Return to top References Unravelling mechanisms of p53-mediated tumour suppression. Bieging KT...early ageing-associated phenotypes. Tyner SD, Venkatachalam S, Choi J, Jones S, Ghebranious N, Igelmann ...

  8. University of Florida Serotype Testing Panel for the Eye and Brain

    Type
    Collection
    ...injection. It was identified by John Chiorini and characterized by Shannon Boye’s lab. AAV44.9(E531D) The AAV44.9...Adeno-Associated Virus Delivered to the Anterior Chamber Results in Efficient Transduction of Trabecular... of glaucoma and their reversed cell entry characteristics. Gene Ther . 2020 Apr;27(3-4):127-142. PMID... AAV44.9-Based Vectors Display Exceptional Characteristics for Retinal Gene Therapy. Mol Ther . Jun 3;...

  9. Luciferase Plasmid Collection

    Type
    Collection
    ...Creation of N-terminal Firefly fusions in Drosopholia Michael Rosbash 128046 pGL3basic luciferase and renilla_polyA...investigating regions controlling transcription Pete Stecha 212933 pGL4.82(hRluc/Puro) Renilla Vector for investigating...investigating regions controlling transcription Pete Stecha 106292 pLS-SV40-mP-Rluc Renilla Insertion of 5'...transfection or viral infection efficiency, to study changes in cell physiology, or as a reference level of ... on protein-protein interactions, drug-induced changes, and more. NanoBit (Link opens in a new window)...

  10. Botman-Teusink Yeast FP Collection

    Type
    Collection
    ...tagging of genes of interest with FPs in yeast. Characterization of some of the FPs in this collection can ... Schmidt P, Goedhart J, Teusink B. In vivo characterisation of fluorescent proteins in budding yeast. ...cassettes for fluorescent protein tagging in Saccharomyces cerevisiae. Yeast. 2004 Jun;21(8):661-70. doi...antibiotic selection markers for engineering in Saccharomyces cerevisiae. Microb Cell Fact. 2013 Oct 25;12...

  11. Addgene Packaged on Request: Scope of Service

    Type
    Collection
    ...Request Service is subject to Addgene’s Terms of Purchase and the applicable material transfer agreements...knowledge from the start. However, we know there’s a chance that things may not turn out as expected. In the...materials. Please be aware that additional customs charges may be required before your order will be delivered...courier will contact you directly regarding these charges, if applicable....

  12. Antibody Production

    Type
    Collection
    ...amplified with primers targeting the heavy chain and/or light chain variable regions. The PCR product is purified...with the plasmid(s) encoding the heavy and light chains. Antibodies are expressed for 7 days and then purified...affinity chromatography, antibodies undergo a buffer exchange using centrifugal columns. The final formulation...

  13. Plan Your Experiment

    Type
    Collection
    ...types of edits, including point mutations, base changes, and large insertions or deletions. For more information...how to validate your genetic edit. Figure 1: Flow chart describing the general framework of a CRISPR experiment...generally higher than HDR pegRNA optimization can be challenging Prime Edit Repress or Interfere (CRISPRi) Reduce...protein domain. Targeting a 5’ exon reduces the chances that the targeted region is removed from the mRNA...interference, and some targeted edits can lead to changes in more than just the DNA sequence. If this is ...

  14. Fluorescent Protein Guide: FRET

    Type
    Collection
    ...to measure protein interactions or conformation changes, as well as FRET standards....intermolecular or bimolecular FRET) or (2) conformational changes within a protein where the donor and acceptor are... Standards The following plasmids have well-characterized FRET efficiency values and were developed to...Brightness standard used as positive control to characterize mTurquoise2 pmTurquoise2-T2A-Venus(L68V) Brightness...

  15. Cre-Lox and Other Site-Specific Recombinases

    Type
    Collection
    ... continue to optimize these tools. The best characterized SSR systems, Cre and Flp, are tyrosine recombinases... Vika. These systems work by sequential strand exchange at a specific target site (called loxP for Cre...genetic control. Recombinase-mediated cassette exchange (RMCE): When matching but mutually incompatible...Zeller Use this dual recombinase-mediated cassette exchange (dRMCE) plasmid kit to re-engineer conditional...

  16. Optogenetics AAV Preps

    Type
    Collection
    ...dependent 5 Bruchas 139505 pAAV-Ef1a-DIO-PPO-Venus EF1a PPO Venus Cre dependent 8, 9 Bruchas 198510 pAAV_hSyn-PdCO-mScarlet-WPRE...our optogenetics AAV collection, including channelrhodopsins, halorhodopsins, and more. Viral... AAV retro helper plasmid from the Karpova and Schaffer labs. See the Retrograde AAV Inventory Page . ...

  17. CRISPR Plasmids - Cascade-Cas3

    Type
    Collection
    ...can be repaired by the cell’s endogenous repair mechanisms. Cas3 belongs to the Class 1 family of CRISPR..., the most abundant type found in bacteria and archaea. While abundant in nature, Class 1 systems are ...components. Figure 1: Overview of Cascade-Cas3 mechanism. Created with BioRender.com. Browse, sort, or ...

  18. SARS-CoV-2 Pseudotyped Virus

    Type
    Collection
    ...Viral Pseudotyping SARS-CoV-2 Pseudotyped Virus Mechanisms of SARS-CoV-2 infection remain poorly understood...This approach allows researchers to safely study mechanisms of infection and to determine methods of neutralizing...of the C-terminal 18-21 amino acids and a D614G change which is present in many circulating variants. ...

  19. CRISPR Plasmids - Base Edit

    Type
    Collection
    ...through base excision repair, creating a C to T change (or a G to A on the opposite strand). Likewise,...guanosine by the cell, creating an A to G (or T to C) change. Adenine DNA deaminases do not exist in nature,...targeted AID-mediated mutagenesis (TAM) from the Chang lab and CRISPR-X from the Bassik lab , in which ...

  20. Microbiology Resources

    Type
    Collection
    ...Browse Plasmids by Microbe System Plasmids for Archaea Species Haloferax sp. Methanococcus maripaludis...application and by color. External Resources European Saccharomyces Cerevisiae Archive for Functional Analysis (...Reagent Program (Link opens in a new window) Saccharomyces Genome Database (SGD) (Link opens in a new window...
Showing: 21 - 40 of 101 results