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Enter a sequence to perform a BLAST-based search of Addgene plasmid sequence databases.

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We narrowed to 98 results for: cha

Showing: 21 - 40 of 98 results

  1. Ras Pathway

    Type
    Collection
    ... protein 35 ARHGEF2 Rho/Rac guanine nucleotide exchange factor 2 CCND CCND1 CCND2 CCND3 Cyclin D CDK CDK4...MLST8 MTOR associated protein, LST8 homolog MTOR Mechanistic target of rapamycin NF1 Neurofibromin 1 NFE2L2...Phosphatidylinositol-3,4,5-trisphosphate-dependent Rac exchange factor 2 PRKA PRKAA1 PRKAA2 PRKAB1 PRKAB2 PRKAG1... RAPGEF RAPGEF1 RAPGEF2 Rap guanine nucleotide exchange factor RAS KRAS MRAS NRAS Kirsten rat sarcoma ...protein SOS SOS1 SOS2 SOS Ras/Rho guanine nucleotide exchange factor SPRED SPRED1 SPRED2 SPRED3 Sprouty related...Tuberous sclerosis VAV1 Vav guanine nucleotide exchange factor 1 YAP1 Yes associated protein 1 Return ...

  2. Optogenetics AAV Preps

    Type
    Collection
    ...dependent 5 Michael Bruchas 139505 pAAV-Ef1a-DIO-PPO-Venus EF1a PPO Venus Cre dependent 8, 9 Michael Bruchas...our optogenetics AAV collection, including channelrhodopsins, halorhodopsins, and more. Viral... AAV retro helper plasmid from the Karpova and Schaffer labs. See the Retrograde AAV Inventory Page . ...

  3. Caltech Systemic Capsids

    Type
    Collection
    ...nlsdTomato Control Fishell 213829 pAAV_BiCHATe27_dTomato_nlsdTomato BiCHATe27 dTomato/nlsdTomato Control Fishell...BiSSTe10 Activator ChR2 Fishell 213830 pAAV_BiCHATe27_ChR2_mCherry BiCHATe27 Activator ChR2 Fishell 213856 pAAV_BiVIPe4...Link opens in a new window) and was adapted from Challis et al. (2022). Text version of the AAV decision...Viviana Gradinaru and cite as follows: PHP.eB, PHP.S: Chan et al., 2017 Pubmed 28671695 (Link opens in a new...

  4. mTOR Pathway

    Type
    Collection
    ...Pathway Plasmids mTOR Gene List Resources The m echanistic or m ammalian t arget o f r apamycin (mTOR) is...mTORC2. Background mTORC1 & mTORC2 The better-characterized mTORC1 responds to various signals, including...known as STK11; serine/threonine kinase 11 mTOR Mechanistic target of rapamycin NF1 Neurofibromin 1 PRAS40...Mitogen-activated protein kinase associated protein 1 mTOR Mechanistic target of rapamycin p53 TP53; tumor protein p53... the translation machinery in cancer. Bhat M, Robichaud N, Hulea L, Sonenberg N, Pelletier J, Topisirovic...

  5. p53 Pathway

    Type
    Collection
    ... mutations have been characterized as gain-of-function, expanding the mechanisms by which mutant p53 is...transcriptional targets is ever-expanding; the best-characterized effects of p53 are in promoting cell cycle arrest...Thrombospondin 1 Return to top References Unravelling mechanisms of p53-mediated tumour suppression. Bieging KT...early ageing-associated phenotypes. Tyner SD, Venkatachalam S, Choi J, Jones S, Ghebranious N, Igelmann ...

  6. University of Florida Serotype Testing Panel for the Eye and Brain

    Type
    Collection
    ...injection. It was identified by John Chiorini and characterized by Shannon Boye’s lab. AAV44.9(E531D) The AAV44.9...Adeno-Associated Virus Delivered to the Anterior Chamber Results in Efficient Transduction of Trabecular... of glaucoma and their reversed cell entry characteristics. Gene Ther . 2020 Apr;27(3-4):127-142. PMID... AAV44.9-Based Vectors Display Exceptional Characteristics for Retinal Gene Therapy. Mol Ther . Jun 3;...

  7. Fluorescent Proteins: FRET

    Type
    Collection
    ...to measure protein interactions or conformation changes....to report on protein binding or conformational changes. Organic dyes, fluorescent proteins, and other ...intermolecular or bimolecular FRET) or (2) conformational changes within a protein where the donor and acceptor are...pairs with mTurquoise2 Mastop et al. (2017) Characterization of a spectrally diverse set of fluorescent...proteins, positive controls for many FRET pairs Michael Davidson Lab (Unpublished) FRET Indicators - Live...

  8. Fluorescent Protein Guide

    Type
    Collection
    ...whole mammals. Michael Davidson Fluorescent Protein Collection Browse the comprehensive Michael Davidson collection...protein-protein interactions or conformational changes within a protein. Biosensors Monitor small biomolecules...protein of interest resides by using a well-characterized fluorescent fusion protein. In Vivo Imaging ...

  9. Luciferase Plasmid Collection

    Type
    Collection
    ...Creation of N-terminal Firefly fusions in Drosopholia Michael Rosbash 128046 pGL3basic luciferase and renilla_polyA...investigating regions controlling transcription Pete Stecha 212933 pGL4.82(hRluc/Puro) Renilla Vector for investigating...investigating regions controlling transcription Pete Stecha 106292 pLS-SV40-mP-Rluc Renilla Insertion of 5'...transfection or viral infection efficiency, to study changes in cell physiology, or as a reference level of ... on protein-protein interactions, drug-induced changes, and more. NanoBit (Link opens in a new window)...

  10. Botman-Teusink Yeast FP Collection

    Type
    Collection
    ...tagging of genes of interest with FPs in yeast. Characterization of some of the FPs in this collection can ... Goedhart, J., Teusink, B. (2019). In vivo characterisation of fluorescent proteins in budding yeast. ...cassettes for fluorescent protein tagging in Saccharomyces cerevisiae. Yeast, 21 (8):661-70. https://doi.org...antibiotic selection markers for engineering in Saccharomyces cerevisiae. Microb Cell Fact, 12 :96. https:...

  11. Addgene Packaged on Request: Scope of Service

    Type
    Collection
    ...Request Service is subject to Addgene’s Terms of Purchase and the applicable material transfer agreements...knowledge from the start. However, we know there’s a chance that things may not turn out as expected. In the...materials. Please be aware that additional customs charges may be required before your order will be delivered...courier will contact you directly regarding these charges, if applicable....

  12. E11 Bio PRISM Collection

    Type
    Collection
    ... J.Y., Chan, S.W., Damstra, H.G.J., Leible, D., Leung, K.S., Magno, C.A., Meeran, A., Michalska, J.M.,...brain tissue. PRISM begins with the delivery of stochastic combinations of cell-filling antigenically distinct...individual synapses. Figure 2: PRISM workflow. A) Stochastic infection of pooled AAVs delivered in vivo generates...

  13. Antibody Production

    Type
    Collection
    ...amplified with primers targeting the heavy chain and/or light chain variable regions. The PCR product is purified...with the plasmid(s) encoding the heavy and light chains. Antibodies are expressed for 7 days and then purified...affinity chromatography, antibodies undergo a buffer exchange using centrifugal columns. The final formulation...

  14. Plan Your Experiment

    Type
    Collection
    ...types of edits, including point mutations, base changes, and large insertions or deletions. For more information...how to validate your genetic edit. Figure 1: Flow chart describing the general framework of a CRISPR experiment...generally higher than HDR pegRNA optimization can be challenging Prime Edit Repress or Interfere (CRISPRi) Reduce...protein domain. Targeting a 5’ exon reduces the chances that the targeted region is removed from the mRNA...interference, and some targeted edits can lead to changes in more than just the DNA sequence. If this is ...

  15. Fluorescent Protein Guide: Empty Backbones

    Type
    Collection
    ...Stokes Shift Photoactivatable Photoconvertible Photoswitchable Timers Self-Labeling Tags Additional Resources...Bacterial Expression Return to top Reversibly Photoswitchable (e.g. Off to On to Off) Protein Excitation ...Fluorescent Timers Fluorescent protein variants that change their fluorescence from the blue to red over time...Kits and Collections Empty Backbone Collection Michael Davidson Fluorescent Protein Collection Botman-...Fluorescent Protein Fusion Construct Kit Neveu Lab MXS Chaining Kit Luciferase Plasmid Collection Promega Plasmid...

  16. Rinehart Lab Phosphoprotein Reagents

    Type
    Collection
    ... CUA. Engineered EF-Tu binds the phosphoserine-charged tRNA (AUC) and is brought to the ribosome where...incorporates phosphoserine into the growing polypeptide chain to generate the new phosphorylated protein. Protein... The Rinehart and Söll labs at Yale University changed the way researchers can explore important questions...improved phosphoprotein yield, rEcoli growth characteristics, and an overall improved ease of use when expressing...

  17. Cre-Lox and Other Site-Specific Recombinases

    Type
    Collection
    ... continue to optimize these tools. The best characterized SSR systems, Cre and Flp, are tyrosine recombinases... Vika. These systems work by sequential strand exchange at a specific target site (called loxP for Cre...genetic control. Recombinase-mediated cassette exchange (RMCE): When matching but mutually incompatible...Zeller Use this dual recombinase-mediated cassette exchange (dRMCE) plasmid kit to re-engineer conditional...

  18. CRISPR Plasmids - Cascade-Cas3

    Type
    Collection
    ...can be repaired by the cell’s endogenous repair mechanisms. Cas3 belongs to the Class 1 family of CRISPR..., the most abundant type found in bacteria and archaea. While abundant in nature, Class 1 systems are ...components. Figure 1: Overview of Cascade-Cas3 mechanism. Created with BioRender.com. Browse, sort, or ...

  19. CRISPR Plasmids - Base Edit

    Type
    Collection
    ...through base excision repair, creating a C to T change (or a G to A on the opposite strand). Likewise,...guanosine by the cell, creating an A to G (or T to C) change. Adenine DNA deaminases do not exist in nature,...targeted AID-mediated mutagenesis (TAM) from the Chang lab and CRISPR-X from the Bassik lab , in which ...

  20. SARS-CoV-2 Pseudotyped Virus

    Type
    Collection
    ...Spike Plasmids Accessory Plasmids Other Resources Mechanisms of SARS-CoV-2 infection remain poorly understood...This approach allows researchers to safely study mechanisms of infection and to determine methods of neutralizing...of the C-terminal 18-21 amino acids and a D614G change which is present in many circulating variants. ...
Showing: 21 - 40 of 98 results