We narrowed to 33 results for: cre encoding plasmid

...cells is to directly transfect expression plasmids encoding your Cas protein and gRNA, which you generally...generate stable cell lines if your plasmid contains a selection marker. Plasmid delivery is versatile thanks...or mRNA encoding the Cas enzyme and gRNA, or as a functional ribonucleoprotein (RNP). Created with BioRender.com...Application Cas Enzyme Additional Considerations Browse Plasmids Knockout Permanently disrupt gene function; cells...different activator systems exist, including the multi-plasmid SAM system Activate Design and Clone Your gRNA ..., anneal, and insert the targeting oligos into plasmids containing the gRNA scaffold using standard restriction-ligation...review the protocol associated with the specific plasmid in question (see CRISPR protocols from Addgene ...

... table highlights plasmid collections that can be used to create and express plasmid-based antibodies....Collections Antibody Plasmid Collection Antibody Plasmid Collection Addgene distributes ready-to-use recombinant...nanobodies). For example, scientists often use plasmids encoding scFv fragments that bind to the GCN4 peptide...Apr 8. Markus Seeger Antibody Plasmids The table below highlights plasmids that feature expression of antibodies...) ID Plasmid Description Gene/Insert PI Return to top Do you have suggestions for other plasmids that ... recombinant antibodies, created by cloning antibody components into plasmids and expressing these in ...collection of plasmid-based antibodies, including monoclonal antibodies, scFvs, and nanobodies. Plasmid...

...and Myers Tagging Plasmids Protein Species Tag Donor Plasmid gRNA plasmid gRNA plasmid FLAG pFETCh_Donor...the remaining 5 plasmids include Cre and FLP expression vectors, a general cloning plasmid, and a prebuilt.... Multiple gRNA plasmids may be used. The HDR (homology directed repair) donor plasmid with homology arms...SapTrap reaction produces a single plasmid targeting vector that encodes both a guide RNA transcript and ...individual tagging event. The kit contains 26 plasmids; 21 of the plasmids are for use in SapTrap reactions for...suggestions for other plasmids that should be added to this list? Fill out our Suggest a Plasmid form or e-mail...This system can be used to create C- and N-terminal epitope tags. The plasmids in the following articles...

...element Activity Cre-dependent Flp-dependent Cre and Flp-dependent Cre, Flp, and VCre-dependent Constitutive... none Cre dependent 1 Kaspar Podgorski 135421 pAAV-syn-FLEX-axon-jYCaMP1s Syn jYCaMP1s none Cre dependent...none Cre dependent 1 GENIE Project 234442 pAAV-syn-flex-iGluSnFR4f-NGR-WPRE Syn iGluSnFr4f none Cre dependent... none Cre dependent 1 Francois St-Pierre 179460 pAAV-EF1a-DIO-JEDI-2P-WPRE EF1a JEDI-2P none Cre dependent...pAAV-Ef1a-Con/Fon/Von GCaMP 6m EF1a GCaMP6m none Cre, Flp and VCre dependent 8 Karl Deisseroth Calcium Sensor...Ready-to-use AAV available from Addgene's viral service encoding biosensor tools, including calcium sensors. Viral...Constitutive (non-cre dependent) Serotype AAV1 AAV2 AAV5 AAV8 AAV9 Retrograde AAV PHPeB Clear Filters ID Name...

...Drosophila ID Plasmid Gene/Insert Promoter Selectable Marker PI Publication Return to top Plant ID Plasmid Gene...selectable marker, and whether the plasmid contains Cas9. ID Plasmid Expression System Promoter Guide RNA...selection of CRISPR plasmids and resources. Find more CRISPR functions along with plasmids categorized by ...suggestions for other plasmids that should be added to this list? Fill out our Suggest a Plasmid form or e-mail...prime editor plasmids for (search and replace) genome editing. CRISPR... CRISPR Prime Edit CRISPR Plasmids: Prime Edit...nickase to the target site. The longer pegRNA also encodes a primer binding site (PBS) and the desired edits...

...empty gRNA plasmids . For more information on CRISPR plasmids and function, see our CRISPR plasmids resource...are made from plasmids that are currently in our repository. You can obtain the plasmid from us or receive...processes. For more information, see our Guide to Plasmid Pooled Libraries . ID Name Description Genes/Insert...N863A) Blasticidin 3rd generation lenti vector encoding dCAS9-VP64 with 2A Blast resistance marker (EF1a-NLS-dCas9... Browse our list of available viruses based on plasmids in our repository that express fluorescent proteins...Pooled CRISPR Libraries for Screening Use pooled CRISPR libraries to screen for genes involved in specific...

... Plasmid Collections Plant Plasmids Plant Plasmids and Resources Cloning & SynBio.... It also includes plasmids encoding silencing suppressors, such as p19. ID Plasmid Description PI Additional...addition to individual plasmids, Addgene carries several useful toolkits and plasmid collections for use ...use in plants. The tables below highlight plasmids and plasmid kits spanning a wide variety of molecular.... ID Plasmid Description Promoter Cloning Method PI Synthetic Biology Kits The following plasmid kits ...full selection of plasmids expressing different functional variants of Cas9. ID Plasmid Description PI Genome... Editing Kits The following plasmid kits and collections contain plasmids designed for genome editing ...

...Rudolf Jaenisch labs Plasmids MECP2 Plasmids The table below highlights plasmids that express or target...information on generating iPSCs using plasmids, please see Addgene's Plasmids for Stem Cell Research page. CRISPR...Broswe plasmids and other research tools for use in Rett Syndrome research. Plasmid... Plasmid Collections Rett ...Rett Syndrome Rett Syndrome Plasmid Collection and Resource Center MECP2 Animal Models Human Cell Lines CRISPR... CRISPR Tools Plasmids Resources The (Link opens in a new window) Rett Syndrome Research Trust (RSRT) ...individual laboratories to assemble a Rett Syndrome plasmid resource for the scientific research community....

... Plasmid Collections Plasmids for Stem Cell Research Plasmids for Stem Cell Research...Reprogramming Plasmids Differentiation and Transdifferentiation Plasmids Stem Cell Research Plasmids Stem cells...utilize plasmids to increase expression of key factors. The tables below will help you find plasmids available... page. Reprogramming Plasmids Browse the table below for plasmids used to create induced pluripotent stem...RNA p53 for single plasmid reprogramming Novel codon-optimized mini-intronic plasmid for efficient, inexpensive...mRNAs encoding human Klf4, Oct4, Sox2, c-Myc, and Lin28A to somatic cells from separate plasmids Highly...Differentiation and Transdifferentiation Plasmids Browse the table below for plasmids used for differentiation of ...

...guide to using CRISPR plasmids in your lab, from knockouts to pooled library screening....multiplexing , and increases the likelihood that any cell containing the CRISPR plasmid will have all desired...discovered. Browse Plasmids: Single-Strand Break (Nick) , Double-Strand Break (Cut) Increasing Cas9 PAM flexibility... cell must be validated experimentally. Browse Plasmids: Double-Strand Break (Cut) Figure 2: Overview ...cases, delivering multiple gRNAs using a single plasmid ensures that all gRNAs will be expressed in the...genetic loci by cloning multiple gRNAs into a single plasmid — though some have achieved targeting in the double...later. Read more about Cas9 multiplexing . Browse Plasmids: Multiplex gRNA Vectors Engineering the Cas9 Endonuclease...

...Find plasmids encoding fluorescent fusion proteins with known subcellular localization patterns for colocalization...suggestions for other plasmids that should be added to this list? Fill out our Suggest a Plasmid form or e-mail... Plasmid Collections Fluorescent Proteins Subcellular Localization...to a red fluorescent protein like mCherry (find plasmids for cloning in our Fluorescent Proteins Empty ...general cellular organelles and structures. ID Plasmid Structure Targeting Gene/Signal Fluorescent Protein...below to find markers of cytoskeletal elements. ID Plasmid Structure Targeting Gene/Signal Fluorescent Protein...below to find markers for cellular junctions. ID Plasmid Structure Targeting Gene/Signal Fluorescent Protein...

...Find GCE synthetase plasmids, target plasmids, and bacterial strains useful for expanding the genetic... Plasmid Collections Genetic Code Expansion Genetic Code Expansion Synthetase Plasmids Target Plasmids...achieve these modifications, two plasmids are generally used. Once these plasmids have been introduced in the... ncAAs. This plasmid often carries multiple tRNA copies for efficiency. A target plasmid containing the...NIH-funded GCE4All Research Center . Synthetase Plasmids Browse plasmids that contain aminoacyl-tRNA synthetases... of these plasmids also contain one or multiple copies of the cognate tRNA gene. ID Plasmid Synthetase...Bacterial TAG Wei Niu Return to top Target Plasmids Browse plasmids that contain genes with modified codons...

...NeuroMab/Trimmer Lab Plasmid Collection R-mAb Plasmids scFv Plasmids Nanobody Plasmids Protocols References...research. Find plasmids encoding these R-mAbs here. Antibody... Antibody Plasmid Collection NeuroMab Plasmid Collection...subclass-specific secondary antibodies. The plasmids encoding these R-mAbs have been deposited with Addgene... the plasmid, followed by the SV40 polyA tail (SV40 pA). Recombinant Monoclonal Antibody Plasmid Collection...following plasmids can be used to produce R-mAbs from transfected mammalian cells. Addgene ID Plasmid Target...partner labs. Learn More scFv Plasmid Collection The following plasmids can be used to produce scFvs from...