...SepOTSγ Plasmid 68285 SepOTSδ Plasmid 68286 SepOTSϵ Plasmid 68287 SepOTSζ Plasmid 68288 SepOTSη Plasmid...SepOTSθ Plasmid 68290 SepOTSι Plasmid 68291 SepOTSκ Plasmid 52054 SepOTSµ ( aka. B40 OTS) Plasmid 68294 ...68294 SepOTSν Plasmid 68295 GFP E17TAG Plasmid 68296 GFP S2TAG Plasmid 68297 GFP Q157TAG Plasmid 68298 GFP...Amp) Plasmid 68300 MBP-MEK1 Plasmid 68301 MBP-MEK1 S218TAG Plasmid 68302 MBP-MEK1 S222TAG Plasmid 68305... 68306 C321.ΔA.Δserb.Amp S Plasmid 68292 SepOTSλ Plasmid 68307 SupD Plasmid 34623 SepOTSα SepRS/EF-Sep...pKD-SepRS-EFSep) Plasmid 34624 SepOTSα tRNA-Sep ( aka. pCAT112TAG-SepT) Plasmid 68283 SepOTSβ Plasmid 68284 SepOTSγ...( 188537 ) is a ROP minus plasmid and is not compatible with other plasmids containing high copy ColE1...

...CRISPR Plasmids: Drosophila Browse CRISPR Plasmids By Function Genome Editing Cut Base Edit Nick Prime...locus and encodes a primer binding site and the desired edits on an RT template. ID Plasmid Gene/Insert...gRNA expression plasmid to target the dCas9-activator to your specific locus. ID Plasmid Gene/Insert Promoter...with a wide variety of Cas-containing plasmids. ID gRNA Plasmid Promoter Cloning Enzyme(s) Delivery Resistance... Cas9 plasmid Bullock and Port 51026 U6-BbsI-crRNA dU6 BbsI Transfection none, need Cas9 plasmid O'Connor-Giles...suggestions for other plasmids that should be added to this list? Fill out our Suggest a Plasmid form or e-mail...CRISPR plasmids that have been designed for use in Drosophila and other insects. CRISPR...

... table highlights plasmid collections that can be used to create and express plasmid-based antibodies....Collections Antibody Plasmid Collection Antibody Plasmid Collection Addgene distributes ready-to-use recombinant...nanobodies). For example, scientists often use plasmids encoding scFv fragments that bind to the GCN4 peptide...Apr 8. Markus Seeger Antibody Plasmids The table below highlights plasmids that feature expression of antibodies...) ID Plasmid Description Gene/Insert PI Return to top Do you have suggestions for other plasmids that ... recombinant antibodies, created by cloning antibody components into plasmids and expressing these in ...collection of plasmid-based antibodies, including monoclonal antibodies, scFvs, and nanobodies. Plasmid...

...and Myers Tagging Plasmids Protein Species Tag Donor Plasmid gRNA plasmid gRNA plasmid FLAG pFETCh_Donor...the remaining 5 plasmids include Cre and FLP expression vectors, a general cloning plasmid, and a prebuilt.... Multiple gRNA plasmids may be used. The HDR (homology directed repair) donor plasmid with homology arms...SapTrap reaction produces a single plasmid targeting vector that encodes both a guide RNA transcript and ...individual tagging event. The kit contains 26 plasmids; 21 of the plasmids are for use in SapTrap reactions for...suggestions for other plasmids that should be added to this list? Fill out our Suggest a Plasmid form or e-mail...This system can be used to create C- and N-terminal epitope tags. The plasmids in the following articles...

...element Activity Cre-dependent Flp-dependent Cre and Flp-dependent Cre, Flp, and VCre-dependent Constitutive...none Cre dependent 1, 5, 9, rg* GENIE 162382 pGP-AAV-CAG-FLEX-jGCaMP8f-WPRE CAG jGCaMP8f none Cre dependent... none Cre dependent 1, 9, rg* GENIE 162380 pGP-AAV-CAG-FLEX-jGCaMP8s-WPRE CAG jGCaMP8s none Cre dependent... none Cre dependent 1, 9, rg* GENIE 162381 pGP-AAV-CAG-FLEX-jGCaMP8m-WPRE CAG jGCaMP8m none Cre-dependent...none Cre dependent 1, rg*, PHPeB Kim 104495 pGP-AAV-CAG-FLEX-jGCaMP7s-WPRE CAG jGCaMP7s none Cre dependent... none Cre dependent 1, 9 Kim , GENIE 100839 pAAV.CAG.Flex.GCaMP6m.WPRE.SV40 CAG GCaMP6m none Cre dependent...none Cre and Flp dependent 8 Deisseroth 137120 pAAV-Ef1a-Con/Foff 2.0-GCaMP6M EF1a GCaMP6m none Cre dependent...

...the tables below for CRISPR prime editing plasmids. Plasmids are available for expression in mammalian...Mammalian ID Plasmid Gene/Insert Promoter Selectable Marker PI Publication Bacteria ID Plasmid Gene/Insert...Plant ID Plasmid Gene/Insert Promoter Selectable Marker PI Publication Drosophila ID Plasmid Gene/Insert...selectable marker, and whether the plasmid contains Cas9. ID Plasmid Expression System Promoter Guide RNA...suggestions for other plasmids that should be added to this list? Fill out our Suggest a Plasmid form or e-mail...prime editor plasmids for (search and replace) genome editing. CRISPR... CRISPR Prime Edit CRISPR Plasmids: Prime Edit...

...efficiency than NHEJ. ID Plasmid Gene/Insert Promoter PI Publication Browse CRISPR Plasmids By Function Genome...locus and encodes a primer binding site and the desired edits on an RT template. ID Plasmid Gene/Insert...gRNA expression plasmid to target the dCas9-activator to your specific locus. ID Plasmid Gene/Insert Promoter...gRNA expression plasmid to target the dCas9-repressor to your specific locus. ID Plasmid Gene/Insert Promoter...with a wide variety of Cas-containing plasmids. ID gRNA Plasmid Promoter Cloning Enzyme(s) Validated In...Ampicillin none, need Cas9 plasmid Qi Do you have suggestions for other plasmids that should be added to ...replaced by guanosine to create A->G (or T->C on the opposite strand) mutations. ID Plasmid Promoter PI Publication...

...CRISPR Plasmids: Mammalian Expression The following CRISPR plasmids have been designed for use in mammalian...NHEJ. ID Plasmid Gene/Insert Promoter Selectable Marker PI Publication Browse CRISPR Plasmids By Function...locus and encodes a primer binding site and the desired edits on an RT template. ID Plasmid Gene/Insert...gRNA expression plasmid to target the dCas9-activator to your specific locus. ID Plasmid Gene/Insert Promoter...gRNA expression plasmid to target the dCas9-repressor to your specific locus. ID Plasmid Gene/Insert Promoter...variety of Cas-containing plasmids. Do you have suggestions for other plasmids that should be added to ...This technique creates diverse populations of mutants for directed evolution. ID Plasmid Gene/Insert Promoter...

... Vectors Encoding Fluorescent Proteins for FRET The following plasmids can be used to create a fluorescent...suggestions for other plasmids that should be added to this list? Fill out our Suggest a Plasmid form or e-mail...studies Addgene has a collection of plasmids suitable for creating individual fluorescently tagged proteins...Addgene's collection of plasmids suitable for FRET experiments to measure protein interactions or conformation... Plasmid Collections Fluorescent Proteins FRET Fluorescent...of interest and the listed fluorescent protein. Plasmid Color Expression Description pPROEX Aqua Cyan Bacterial... to Top FRET Reference Standards The following plasmids have well-characterized FRET efficiency values...

...locus and encodes a primer binding site and the desired edits on an RT template. ID Plasmid Gene/Insert...gRNA expression plasmid to target the dCas9-activator to your specific locus. ID Plasmid Gene/Insert Promoter...gRNA expression plasmid to target the dCas9-repressor to your specific locus. ID Plasmid Gene/Insert Promoter...paired with a wide variety of Cas-containing plasmids. ID Plasmid Promoter Cloning Enzyme Co-expressed Cas9...suggestions for other plasmids that should be added to this list? Fill out our Suggest a Plasmid form or e-mail...replaced by guanosine to create A->G (or T->C on the opposite strand) mutations. ID Plasmid Gene/Insert Promoter...expression plasmids that have been designed for use in plants. CRISPR...

...Browse Tetracycline Plasmids Tet-regulated Lentiviral RNAi Vectors Curated Plasmid Collections Background...interest tTA expression plasmid Components for a tetracycline on system are: Plasmid containing a tetracycline-dependent...generation lentiviral plasmid for inducible expression of shRNA; neomycin selection; plasmid 21915 has puromycin...suggestions for other plasmids that should be added to this list? Fill out our Suggest a Plasmid form or e-mail... Plasmid Collections Tetracycline (Tet) Inducible Expression...Tetracycline Off Tetracycline On Experimental Tips Tet Plasmids Background To advance the study of gene function...uptake. Search our collection for all tetracycline plasmids. Tet System and the Tet Response Element (TRE)...

...nucleocapsid protein (Brian Geiss). Plasmids SARS-CoV-2 Plasmids Many plasmids containing SARS-CoV-2 sequences...Addgene will maintain this plasmid collection page, which highlights plasmids available that may be useful...SARS-CoV-2 nucleocapsid protein. SARS-CoV-2 Plasmids : Plasmids that are available or coming soon containing...containing SARS-CoV-2 sequences. SARS-CoV Plasmids : Plasmids that are available containing SARS-CoV (SARS ...to popular envelope and packaging plasmids. Ginkgo Bioworks Plasmid Collection - Addgene has partnered...Bioworks to distribute SARS-CoV-2 plasmids. The collection contains: Plasmids for E. coli and Yeast expression...available Ginkgo SARS-CoV-2 plasmids are curated on a separate Ginkgo Bioworks Plasmid Collection page. SARS-CoV...

...Rudolf Jaenisch labs Plasmids MECP2 Plasmids The table below highlights plasmids that express or target...information on generating iPSCs using plasmids, please see Addgene's Plasmids for Stem Cell Research page. CRISPR...Broswe plasmids and other research tools for use in Rett Syndrome research. Plasmid... Plasmid Collections Rett ...Rett Syndrome Rett Syndrome Plasmid Collection and Resource Center MECP2 Animal Models Human Cell Lines CRISPR... CRISPR Tools Plasmids Resources The (Link opens in a new window) Rett Syndrome Research Trust (RSRT) ...individual laboratories to assemble a Rett Syndrome plasmid resource for the scientific research community....

...Reporter Plasmids You may also like… Plasmids 101 Addgene's Molecular Biology Guides Plasmids 101: E. ...interest. Plasmid ID Promoter Inducer(s) PI Purpose Murray Lab pBEST plasmids Various Plasmids pBAD, OR2...Purification Controlled Expression Reporter Plasmids Reporter Plasmids Tagging and Visualization Purification...list of our bacterial CRISPR plasmids, see here . pGRG Series Various Plasmids Tn7 Transposition Nancy Craig...green light. BIOFAB plasmid set 1000000037 N/A N/A Drew Endy This kit contains plasmids with various combinations...specific level. Return to Top Reporter Plasmids Reporter plasmids can be used to detect events and molecules...the reporter gene. Plasmid ID Promoter Reporter PI Purpose pRU Series Various Plasmids Cloned by researcher...

... Plasmid Collections Plasmids for Stem Cell Research Plasmids for Stem Cell Research...Reprogramming Plasmids Differentiation and Transdifferentiation Plasmids Stem Cell Research Plasmids Stem cells...utilize plasmids to increase expression of key factors. The tables below will help you find plasmids available... page. Reprogramming Plasmids Browse the table below for plasmids used to create induced pluripotent stem...RNA p53 for single plasmid reprogramming Novel codon-optimized mini-intronic plasmid for efficient, inexpensive...mRNAs encoding human Klf4, Oct4, Sox2, c-Myc, and Lin28A to somatic cells from separate plasmids Highly...Differentiation and Transdifferentiation Plasmids Browse the table below for plasmids used for differentiation of ...

...Plasmids encoding fluorescent proteins tagged with genes or peptides with known subcellular localization...suggestions for other plasmids that should be added to this list? Fill out our Suggest a Plasmid form or e-mail...table below to find markers of secretory pathway components. ID Plasmid Structure Targeting Gene/Signal... Plasmid Collections Fluorescent Proteins Organelle Markers...Empty Backbones Allen Institute for Cell Science Plasmid Collection FRET Biosensors Optogenetics Fluorescent...general cellular organelles and structures. ID Plasmid Structure Targeting Gene/Signal Fluorescent Protein...below to find markers of cytoskeletal element. ID Plasmid Structure Targeting Gene/Signal Fluorescent Protein...

...guide to using CRISPR plasmids in your lab, from knockouts to pooled library screening....multiplexing , and increases the likelihood that any cell containing the CRISPR plasmid will have all desired...discovered. Browse Plasmids: Single-Strand Break (Nick) , Double-Strand Break (Cut) Increasing Cas9 PAM flexibility... cell must be validated experimentally. Browse Plasmids: Double-Strand Break (Cut) Multiplex Genome Engineering...cases, delivering multiple gRNAs using a single plasmid ensures that all gRNAs will be expressed in the...genetic loci by cloning multiple gRNAs into a single plasmid — though some have achieved targeting in the double...later. Read more about Cas9 multiplexing . Browse Plasmids: Multiplex gRNA Vectors Engineering the Cas9 Endonuclease...

...Species) Organism/Expression System Plasmids Mammalian Golenbock Lab Plasmids - Includes tagging with CFP, YFP...Lentiviral Plasmids - Fluorescently tag your gene for lentiviral expression Verkhusha Lab Plasmids - Enhanced...Mammalian Plasmids - Includes tagging with mCherry, mCitrine, mCerulean Davidson Lab Plasmids - Includes...suggestions for other plasmids that should be added to this list? Fill out our Suggest a Plasmid form or e-mail...collection of empty plasmid backbones with different fluorescent tags for you to create fusion proteins with...Background Fluorescent proteins are genetically encoded tools that are used extensively by life scientists...

...Generally, you will need two plasmids, as depicted in the figure below: A plasmid expressing the tRNA and ... included. Browse Synthetase Plasmids The table below highlights plasmids that contain aminoacyl tRNA ...Many of the plasmids also contain one or more copies of the cognate tRNA gene. ID Plasmid Synthetase Origin...Abhishek Chatterjee Browse Target Plasmids The table below highlights plasmids that contain genes with modified...Addgene's guide to plasmids and strains useful for expanding the genetic code in bacteria and mammalian... Plasmid Collections Genetic Code Expansion Genetic Code Expansion You may also like... GCE4All... genetic code by redirecting specific codons to encode amino acids beyond the 20 standard amino acids....

...NeuroMab/Trimmer Lab Plasmid Collection R-mAb Plasmids scFv Plasmids Nanobody Plasmids Protocols References...research. Find plasmids encoding these R-mAbs here. Antibody... Antibody Plasmid Collection NeuroMab Plasmid Collection...subclass-specific secondary antibodies. The plasmids encoding these R-mAbs have been deposited with Addgene... the plasmid, followed by the SV40 polyA tail (SV40 pA). Recombinant Monoclonal Antibody Plasmid Collection...following plasmids can be used to produce R-mAbs from transfected mammalian cells. Addgene ID Plasmid Target...partner labs. Learn More scFv Plasmid Collection The following plasmids can be used to produce scFvs from...