We narrowed to 143 results for: ins;

...Plasmid Gene/Insert Vector Type PI Return to top Bacterial Strains Browse bacterial strains that have been...synthetase plasmids, target plasmids, and bacterial strains useful for expanding the genetic code in bacteria... Synthetase Plasmids Target Plasmids Bacterial Strains The genetic code for all life is based upon four...properties for studying, manipulating, and evolving proteins. Expanding the Genetic Code During protein translation...genetic code can help us understand and evolve proteins for various purposes from therapeutics to biopolymers... (RF1 is deleted); T7RNAP cassette genomically inserted Michael Jewett 182777 C321.∆A T7RNAP ompT- rne... (RF1 is deleted); T7RNAP cassette genomically inserted; ompT and rne inactivated Michael Jewett 182778...

...or MCP tagged plasmids for visualization of proteins inside cells....-, ACP-, and MCP-tags for visualization of proteins inside living or fixed cells. The plasmids associated... labeling cell-surface proteins, and are useful for labeling secreted proteins with disulfide bridges ...features: Allows for visualization of multiple proteins simultaneously with dual labeling when used with...live or fixed cells, and for internal or surface proteins Single constructs are compatible with multiple... and MCP-tags are used exclusively for surface proteins and use enzymatic binding to attach substrate ...suitable for both cell-permeant and non-cell-permeant proteins. CLIP-tag CLIP-tag is identical in function to...

...plasmid — contains transgene, sgRNA, or shRNA of interest flanked by LTRs Packaging plasmid — contains packaging...plasmids in third-generation Envelope plasmid — contains packaging gene env This collection highlights ...1alpha-driven constitutive transgene expression. Contains intron that gives high expression. Didier Trono...and Paul Kaufman 102930 pLJC5-Tmem192-3xHA 3rd Contains a lysosomal tag. See Abu-Remaileh et al. (2017...expression of GFP. Jason Sheltzer 20342 FUW-M2rtTA 3rd Contains reverse tetracycline transactivator. Rudolf Jaenisch...and rev . Garry Nolan 8455 pCMV-dR8.2 dvpr 2nd* Contains gag , pol , rev , tat , vif , and nef . Can be...information. Bob Weinberg 12251 pMDLg/pRRE 3rd Contains gag and pol . Can be used with 3rd generation ...

... Gene/Insert Promoter Selectable Marker PI Publication Return to top Cut ID Plasmid Gene/Insert Promoter...Plasmid Gene/Insert Selectable Marker PI Publication Return to top Interfere ID Plasmid Gene/Insert Promoter...Gene/Insert Promoter Selectable Marker Publication Return to top Prime Edit ID Plasmid Gene/Insert Promoter... Gene/Insert Selectable Marker PI Publication Return to top RNA Knockdown ID Plasmid Gene/Insert Promoter...table for "Mammalian". Activate ID Plasmid Gene/Insert Promoter Selectable Marker PI Publication Return...Publication Return to top Purify ID Plasmid Gene/Insert Promoter Selectable Marker PI Publication Return...Publication Return to top Visualize ID Plasmid Gene/Insert Promoter Selectable Marker PI Publication Return...

...small tag insertions, by copying from a DNA donor template Cas9 or Cas9 fusions with proteins that promote...including point mutations, base changes, and large insertions or deletions. For more information on the wide...our CRISPR Guide . There are many different Cas proteins available, and researchers can adapt these experiments...Cas9 nickase fusions with different base editing proteins) Many base editors fused to Cas9 exert their activity...sequence Base Edit Prime Edit Generate targeted insertions, deletions, and point mutations using a prime... In many cases, you can synthesize, anneal, and insert the targeting oligos into plasmids containing the...can generate stable cell lines if your plasmid contains a selection marker. Plasmid delivery is versatile...

...Gene/Insert Promoter Selectable Marker PI Publication Return to top Bacteria ID Plasmid Gene/Insert Promoter...Gene/Insert Promoter Selectable Marker PI Publication Return to top Plant ID Plasmid Gene/Insert Promoter...Gene/Insert Promoter Selectable Marker PI Publication Return to top Yeast ID Plasmid Gene/Insert Promoter...Gene/Insert Promoter Selectable Marker PI Publication Return to top Xenopus ID Plasmid Gene/Insert Promoter...joining (NHEJ), a mechanism which frequently causes insertions or deletions (indels) in the DNA. Indels often...repair a DSB using homology-directed repair (HDR) instead of NHEJ. In most experimental systems, HDR occurs...Publication Return to top Parasite ID Plasmid Gene/Insert Promoter Selectable Marker PI Publication Return...

... System Contains 336 pFusX clones allowing for Golden Gate assembly of 15-16 RVD TALEN inserts in one ...plasmids of pre-assembled trimer and tetramer RVD domains designed for rapid TALEN assembly. Kiran Musunuru...message. Both vectors utilize homodimeric FokI domains. 38143 pC-GoldyTALEN Golden Gate TALEN 2.0 36033...sequences within core promoter regions. pTAL5-BB contains the GAL1 promoter, placing TALORs built into this...under galactose-inducible expression. pTAL6-BB contains the TEF1 promoter, giving constitutive expression...mutation induction when using TALENs. The FokI domains (DD, RR) used are obligate heterodimers that require...require cloning of left and right TALEN monomer proteins into opposite vectors. 37276 pCS2TAL3-RR 48636...

... Carrier Proteins (SLCs) from the RESOLUTE collection AAV Packaging from Addgene Parkinson's Research ...SARS-COV-2, ACE2, and CRISPR tools Fluorescent Proteins from the Michael Davidson collection Protein Labeling...available to industry scientists. ID Plasmid Gene/Insert PI Kits Kit name Type PI Description High Complexity... constructs. ONE-GO Biosensors Kit Fluorescent Proteins Mikel Garcia-Marcos 10 plasmids for detecting ...Bahl A validation set of 96 unique, single-domain proteins that are structurally characterized (PDB-deposited...Screening Messer and MJFF Created for research in Parkinson's Disease; For target validation and drug design...agreement between the recipient and the depositing institution(s). It governs the transfer of biological materials...

...Genetics 2014 Allen Institute for Cell Science Plasmid Collection The Allen Institute for Cell Science has...CRISPR system has been adapted to allow tagging of proteins expressed from their natural chromosomal context...add a tag (currently using FLAG) to endogenous proteins. This CRISPR/Cas plasmid-system consists of two...2A-PuroR cassette to the C-terminus of endogenous proteins. The PITCh system depends on MMEJ (microhomology-mediated...end-joining), an alternative DSB repair pathway, instead of HDR, which makes it easy to construct a donor...'s lab for building CRISPR targeting vectors to insert genetic tags in the C. elegans genome. The SapTrap...template for an individual tagging event. The kit contains 26 plasmids; 21 of the plasmids are for use in...

...of interest, a medium copy RSF1030 origins or a low copy p15a origins should be used. Find further details...Mode #2 phosphosite library contains four different phosphobinding domains: 14-3-3β, 14-3-3σ, the WW2 ...orthogonal translation system to make phosphorylated proteins with one or more phosphorylation sites (Pirman...improved ease of use when expressing recombinant phosphoproteins. These attributes make rEcoli XpS (Bacterial...other plasmids containing high copy ColE1/PUC-like origins. Therefore, when choosing plasmids to express the...sequences corresponding to every previously observed instance of human serine phosphorylation ( Rinehart Human...#68307) ) to generate the phosphosites with serine instead of phosphoserine (i.e., a “phosphorylatable” phosphosite...

...our repository that express fluorescent proteins. ID Name Insert Antibiotic Resistance Purpose PI 17446...Plasmid Pooled Libraries . ID Name Description Genes/Insert Selection PI Human knockout pooled libraries 73178...with 1000 non-targeting controls. This backbone contains SpCas9. SpCas9 and 76,441 unique sgRNAs targeting...interrogate the genome in a variety of ways. Cas9 proteins can be guided to specific genomic sites by an ... ) or single-stranded breaks ( nicks ). ID Name Insert Function Antibiotic Resistance Activity PI 52962...and used as a transcriptional activator . ID Name Insert Antibiotic Resistance Activity PI 61422 dCAS9-VP64...barcoding library. Version 1 of the Celltag library contains 19973 barcodes to combinatorially index cells ...

...Protein Guide Fluorescent Proteins: Plasmids and Resources Addgene's repository contains thousands of fluorescent...Addgene Blog: Fluorescent Proteins Fluorescent Protein Plasmid Kits Allen Institute for Cell Science Plasmid...to learn about the applications of fluorescent proteins and to find the plasmids that are available from...more about the many applications of fluorescent proteins and to find plasmids for your next experiment....collection of genes and markers tagged with fluorescent proteins as well as hundreds of empty backbones. FRET Find...subcellular structures with well-characterized marker proteins. In Vivo Imaging Image with far-red or infrared...open-source, community-editable database for fluorescent proteins created by Talley Lambert. Nikon MicroscopyU (...

...bidirectional insertions — meaning they may not be suitable for applications where orientation of the insert is ... almost unidirectional insertions, but are more prone to off-target insertions. CASTs are most often used...Gene/Insert Promoter Selectable Marker PI Publication Return to top Bacteria ID Plasmid Gene/Insert Selectable...for CRISPR-associated transposases (CASTs) for insertion of donor DNA. CRISPR...locations. The Cas effector lacks nuclease activity, instead recruiting the transposase (comprised of multiple...is important. However, type I-F CASTs favor insertion in the T-RL direction, with the right homology end...

...with large inserts Vogelstein 1000000176 AdenoBuilder Toolkit Plasmids containing modular inserts that span...reaction Bunz Return to top Adenoviral Strains Browse bacterial strains for adenoviral vector production. ...plasmids, adenoviral backbone plasmids, and bacterial strains for adenoviral vector production in Addgene's repository...Shuttle Plasmids Adenoviral Plasmids Adenoviral Strains Adenoviral vectors are widely used for gene delivery... Search our database for shuttle plasmids with inserts. ID Plasmid Description PI 16402 pShuttle For production...16407 pAdEasy 2-GFP beta-gal Test plasmid that contains β-gal and GFP and a ∼10 Kb genomic DNA stuffer...Vogelstein 16399 AdEasier-1 BJ5183 derivative that contains the pAdEasy-1 (Addgene #16400) plasmid Vogelstein...

...plasmid — contains transgene, sgRNA, or shRNA of interest flanked by LTRs Packaging plasmid — contains packaging...pUMVC Contains MoMLV gag and pol . Use with an envelope plasmid. Bob Weinberg 12371 pCL-Eco Contains MoMLV...includes a reverse transcriptase and an integrase to insert the viral genome into a host cell. Scientists can...packaging genes Envelope plasmid — contains packaging gene env This collection highlights popular gamma-...database for retroviral transfer plasmids that have inserts. ID Plasmid 5'LTR Description PI 1780 pBABE-neo...modified version of pRX-tight Puro encoding NAT instead of PAC. James Iglehart 20672 MSCV-IRES-GFP MSCV...pol , and env . Inder Verma 35614 pBS-CMV-gagpol Contains MoMLV gag and pol . Patrick Salmon Return to top...

...Collections Fluorescent Proteins Subcellular Localization Fluorescent Proteins: Subcellular Localization...responsible for delivering proteins to the extracellular environment. Proteins are first translated at the...Find plasmids encoding fluorescent fusion proteins with known subcellular localization patterns for colocalization...assembled a collection of popular fluorescent fusion proteins that are targeted to various organelles or subcellular... (find plasmids for cloning in our Fluorescent Proteins Empty Backbones Collection ). Then, by examining...p63 mTurquoise2 Dorus Gadella 45944 pTDpelB-C_sfYFPTwinStrep Periplasmic space PelB signal sequence YFP...F-tractin-EGFP Actin filaments F-tractin EGFP Dyche Mullins 41147 pEGFP Centrin2 Centrioles (dependent on cell...

...nickase. The fusion enzyme is capable of installing targeted insertions, deletions, and point mutations using...Gene/Insert Promoter Selectable Marker PI Publication Return to top Bacteria ID Plasmid Gene/Insert Promoter...Gene/Insert Promoter Selectable Marker PI Publication Return to top Plant ID Plasmid Gene/Insert Promoter...edited 3’ flap is excised, the target sequence remains unchanged and available as a substrate for another...small prime editors with optimized RT and/or Cas9 domains PE7 — addition of RNA-binding exonuclease protection...enzyme, selectable marker, and whether the plasmid contains Cas9. ID Plasmid Expression System Promoter Guide...

...ID Plasmid Description Gene/Insert PI Plasmids Expressing Worm Genes/Inserts The table below lists plasmids... vectors for tag insertion using CRISPR/Cas9 and the selection of C. elegans strains. CRISPR plasmids ...includes CRISPR, RNAi, empty backbones, fluorescent proteins, and Cre expression plasmids. Large collections...briggsae , etc.) gene or sequence: ID Plasmid Gene/Insert PI Don’t see your gene listed? Know of some great...elegans genome engineering - Bob Goldstein Lab. Contains a set of plasmids for building homologous repair...Transgenesis Kit - Erik Jorgensen Lab. This kit contains 29 plasmids used to generate transgenic nematodes...Caenorhabditis Genetics Center (CGC) - The CGC collects, maintains, and distributes stocks of C. elegans. Silencing...

... Plasmid Collections Fluorescent Proteins In Vivo Imaging Fluorescent Proteins: In Vivo...Additional Resources Fluorescent Proteins: Empty Backbones Fluorescent Proteins: Subcellular Localization Luciferase...fluorescent proteins. Plasmid...Fluorescence microscopy is a powerful tool to study proteins and cells, but imaging in deep-tissue organs or...unique challenges. Genetically-encoded fluorescent proteins most suitable for stable imaging in live animals...background signals. Many near-infrared fluorescent proteins are derived from bacterial phytochrome photoreceptors...

...developed class of tools are Luminopsins (LMOs), or luminescent opsins: fusion proteins of a light-emitting luciferase...specific biological processes. Addgene's collection contains two main classes of chemogenetic receptors: Designer...muscarinic receptors and couple to the Gi, Gq, or Gs G-proteins, respectively. KORD is derived from the κ-opoid...engineered α7 nicotinic acetylcholine receptor (nAChR) domains that respond specifically to small molecules termed...chemogenetics plasmids. ID Plasmid Description Gene/Insert Vector Type Promoter Eligible for Viral Packaging...Chemogenetics Fluorescent Biosensors Fluorescent Proteins Guide Optogenetics Plasmids Optogenetics Guide...