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Showing: 21 - 28 of 28 results

  1. CRISPR Plasmids - Tagging

    Type
    Collection
    ...gene-specific CRISPR and donor vectors as well as pX330S-2-PITCh, expressing a generic gRNA targeting the PITCh...Protocols, 2016 . Yamamoto Tagging Plasmids: pX330S-2-PITCh - expresses Cas9 & PITCh-gRNA pX330A-FBL/PITCh -...conventional HDR-mediated gene knock-in. The detailed procedure can be found in Sakuma et al., Nature Protocols...

  2. CRISPR References and Information

    Type
    Collection
    ...Mammalian Cell Lines CasPEDIA : An encyclopedia of Class 2 CRISPR systems with wiki entries describing enzyme...library amplification 1 vector system: lentiCRISPR v2 2 vector system: lentiCas9-Blast and lentiGuide-Puro...families, HDR oligonucleotide design, and batch processing for designing genome-wide gRNA libraries. PubMed...

  3. Deisseroth INTRSECT Collection

    Type
    Collection
    ...dual-transgenic recombinase-expressing mouse lines 2–9 and combinations of transgenic recombinase-expressing...delivering additional recombinases 10–14 . Figure 2: INTRSECT works by inserting short, intronic sequences... present, the introns are excised during mRNA processing, producing a functional molecular tool (C,F)....Deisseroth Lab maintains a Standard Operating Procedure (Link opens in a new window) with general principles...

  4. CRISPR Plasmids - Empty gRNA Vectors

    Type
    Collection
    ...Cas enzymes? CasPEDIA is an encyclopedia of Class 2 CRISPR systems with wiki entries describing enzyme...BclI-SwaI none S. pyogenes URA3 Wyrick pCRISPomyces-2 61737 Bacteria BbsI yes, cut S. pyogenes Apramycin... S. pyogenes Zhang gRNA Cloning Vector Bbs I ver. 2 85586 Mammalian BbsI None S. pyogenes H Fujii pX601...with the endogenous tRNA-processing system Plant Utilizes the tRNA processing system to reduce size and...Editing in Monocot and Dicot Plants Plant PCR-based procedure to rapidly generate multiple sgRNA expression ...Bullock pCFD5 Drosophila Utilizes endogenous tRNA processing machinery to excise multiple functional gRNAs...

  5. Antibody Production

    Type
    Collection
    ...for shipping stability by incubating at 37 °C for 2 weeks, freezing at -80 °C, thawing on ice, and then...antibody production methods and quality control processes for purified recombinant antibodies. Antibody...

  6. Luciferase Plasmid Collection

    Type
    Collection
    ...fusing NanoLuc® and Venus fluorophore to Troponin C Ca 2+ binding domain. Nano-lanterns , Enhanced Nano-lanterns...transfection Feng Zhang 21471 pLenti PGK V5-LUC Neo (w623-2) Firefly PGK Lentiviral expression of firefly luciferase...BRET-based in vivo imaging of multiple cellular processes, biosensors, or optogenetic tools . pcDNA3.1+-...used for screening of transformants in cloning process. Lynne Postovit 119760 pSGDlucV3.0 Firefly/Renilla...

  7. Zhang Lab's CRISPR Frequently Asked Questions

    Type
    Collection
    ...extraction solution, runs the protocol, then uses around 2-4ul in each 50ul PCR reaction. The gDNA extracted ...facilitate the processing of the crRNA) is also expressed from a separate promoter. In the pre-processing form,...construct from our 'Split RNA' design, where the pre-processing form of crRNA is expressed and a tracrRNA (the...in cell and with Cas9 and tracrRNA, it will be processed (i.e., cleaved) into a shorter form that contains...our 'Chimeric RNA' design (meaning the mature processed form of crRNA is fused chimerically to a mature...

  8. Plan Your Experiment

    Type
    Collection
    ...either (1) a lack of gRNA and/or Cas9 expression or (2) a lack of efficient target cleavage in cells expressing...is necessary to model your specific disease or process of interest. Do you want to: Generate complete ... method of delivery and the cell type. Before proceeding with your experiment, it may be necessary to ...
Showing: 21 - 28 of 28 results