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Addgene

Enter a sequence to perform a BLAST-based search of the Addgene plasmid sequence database.

Information

In Category

We narrowed to 43 results for: POR C

Showing: 41 - 43 of 43 results

  1. Genetic Code Expansion

    Type
    Collection
    ...synthetase subunit E. coli p-azido-L-phenylalanine (Azf) C. elegans ATG David Tirrell 62599 pKPY197 phenylalanyl-tRNA...(CePheRS) C.elegans p-azido-L-phenylalanine (Azf) C. elegans ATG David Tirrell 63177 pMarsL274G methionyl-tRNA... the codon), the amino acid from the tRNA is incorporated into the growing peptide chain. To expand the...aminoacyl-tRNA-synthetase (aaRS) that has been evolved to incorporate non-canonical amino acids (ncAAs). A plasmid ...the cells, the non-canonical amino acid can be incorporated using the existing protein translation machinery...translation machinery are needed in order to incorporate a non-canonical amino acid into the protein of... the place where the new amino acid would be incorporated. Other options, such a 4-base pair codons, have...

  2. CRISPR References and Information

    Type
    Collection
    ...activation). Genome support for bacteria ( E. coli, B. subtilis ), yeast ( S. cerevisiae ), worm ( C. elegans )...gRNA and HDR donor design. The current version supports SpCas9, SpCas9-VQR, and enAsCas12a Cas enzymes...Lab . Read more about it on the Addgene blog . CRISPOR (Link opens in a new window) This tool helps design...(2016) (Link opens in a new window) . Also try CRISPOR batch (Link opens in a new window) for lentiviral...input sequence and checks for off-target binding. Supports a range of Cas enzymes (Cas9 and Cas12) and organisms... or a sequence text file you upload. Currently supports a wide selection of organisms. From the Database...sequence and checks for off-target binding. Currently supports a wide range of organims. Developed by the Michael...

  3. Plan Your Experiment

    Type
    Collection
    ...) may be more efficacious than gRNAs containing a C nucleotide at the same position in spite of being ...promoter is typically used for gRNA May contain reporter gene (e.g. GFP) to identify and enrich positive...vector or separate transfer vectors May contain reporter gene (e.g. GFP) or selection marker to identify... to target cells through microinjection or electroporation Transient expression of CRISPR components Expression...may alter protein function when they occur in important protein domains. For gene editing experiments ... addition to off-target activity , it is also important to consider factors that maximize cleavage of ...25408407 Hashimoto, M., & Takemoto, T. (2015). Electroporation enables the efficient mRNA delivery into the...
Showing: 41 - 43 of 43 results