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We narrowed to 52 results for: gfp gene

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Showing: 41 - 52 of 52 results

  1. Neurodegeneration Research Collection

    Type
    Collection
    ...type or mutant ORFs, gene fragments, and gRNAs for genes linked to neurodegenerative disorders. Find plasmids...within the huntingtin (HTT) gene. An individual with the mutated HTT gene will have huntingtin proteins...association between a gene and late-onset AD. It is more likely a combination of gene variation and lifestyle...Localization , Optogenetics Plasmids , and Chemogenetics Plasmids . Find plasmids for endogenous gene tagging... are associated with known genetic mutations in one of the following genes: SOD1, C9ORF72, TARDBP, FUS...these additional genes may play in Alzheimer's disease. Research Tools Neurodegeneration Plasmid Collection...heritable gene silencing with CRISPRoff-v2.1 . (Nuñez et al. Cell. 2021) Conduct multimodal genetic screens...

  2. Biosensor AAV Preps

    Type
    Collection
    ...pAAV-CAG-FLEX-Archon1-KGC-EGFP-ER2 CAG Archon1 EGFP Cre dependent 5 Edward Boyden 115892 pAAV-Syn-Archon1-KGC-GFP-ER2 Syn...Syn Archon1 EGFP Constitutive 8 Edward Boyden 115893 pAAV-Syn-FLEX-rc [Archon1-KGC-GFP-ER2] Syn Archon1...HaloCaMP1a 138327 pAAV-synapsin-HaloCaMP1a-EGFP Syn HaloCaMP1a EGFP Constitutive 1, 9 Eric Schreiter Calcium...HaloCaMP1b 138328 pAAV-synapsin-HaloCaMP1b-EGFP Syn HaloCaMP1b EGFP Constitutive 1, 9 Eric Schreiter Dopamine...Ready-to-use AAV available from Addgene's viral service encoding biosensor tools, including calcium sensors...Archon1 EGFP Cre dependent 8 Edward Boyden Voltage Reporter: Voltron 119036 pAAV-hsyn-flex-Voltron-ST Syn Voltron-ST...

  3. Cre-Lox and Other Site-Specific Recombinases

    Type
    Collection
    ...excised, and gene expression proceeds. Gene Knockout: Placing the loxP sites on either side of a gene (called...shRNA expression. Gene Switch: These constructs contain two genes of interest, Genes A and B. When Cre... few popular recombinase-dependent gene expression strategies. Gene Expression: A stop codon flanked with...LSL" cassette) placed upstream of a gene of interest will prevent gene expression in the absence of Cre.... loxP"), will permit gene expression until Cre is present, at which time the gene will be disrupted or...Cre is absent, only Gene A is translated correctly. Cre expression excises Gene A and alters the reading...backbone to regulate your gene of interest. Depending on the construct, gene expression may be activated...

  4. Allen Institute for Cell Science Plasmid Collection

    Type
    Collection
    ... Endogenous Gene Tagging in Human Cells on the Addgene Blog. Plasmids for Endogenous Gene Tagging in Human...Link opens in a new window) RNP transfection for gene editing human iPS cells (Link opens in a new window..., S. M., & Gunawardane, R. N. (2017). Systematic gene tagging using CRISPR/Cas9 in human stem cells to...87421 TUBA1B-mEGFP AICSDP-4 mEGFP Alpha-tubulin Microtubules 87422 LMNB1-mEGFP AICSDP-10 mEGFP Lamin B1 Nuclear...envelope 87423 TOMM20-mEGFP AICSDP-8 mEGFP Tom20 Mitochondria 87424 DSP-mEGFP AICSDP-9 mEGFP Desmoplakin Desmosomes...87425 ACTB-mEGFP AICSDP-15 mEGFP Beta-actin Actin filaments 87426 SEC61B-mEGFP AICSDP-7 mEGFP Sec61 beta...101782 LAMP1-mEGFP AICSDP-19 mEGFP LAMP-1 Lysosome 101783 MAP1LC3B-mEGFP AICSDP-25 mEGFP Autophagy-related...

  5. The Pleiades Promoter Project

    Type
    Collection
    ...MiniPromoter Source Gene Construct Reporter Ple2 ADORA2A pEMS1142 EGFP/NLS Ple3 ADORA2A pEMS1143 EGFP/NLS Ple3 ADORA2A... disorders by enabling region- and cell-specific gene-delivery in the mouse brain through these human ...pEMS1153 EGFP/NLS Ple37 CRH pEMS1154 EGFP/NLS Ple38 CRH pEMS1155 EGFP/NLS Ple39 CRH pEMS1156 EGFP/NLS Ple44...pEMS1113 EGFP/cre/NLS Ple51 DBH pEMS1362 EGFP/NLS Ple52 DCX pEMS1198 EGFP/NLS Ple53 DCX pEMS1199 EGFP/NLS ...pEMS1167 EGFP/NLS Ple63 DRD1 pEMS1168 EGFP/NLS Ple64 FEV pEMS1129 EGFP/cre/NLS Ple64 FEV pEMS1398 EGFP/NLS ...pEMS1160 EGFP/NLS Ple93 GPR88 pEMS1161 EGFP/NLS Ple94 GPR88 pEMS1162 EGFP/NLS Ple95 GPR88 pEMS1163 EGFP/NLS...pEMS1216 EGFP/NLS Ple99 GRP pEMS1371 EGFP/NLS Ple100 GRP pEMS1372 EGFP/NLS Ple101 GRP pEMS1373 EGFP/NLS Ple102...

  6. Kazuhiro Oka Lentiviral Vectors

    Type
    Collection
    ... Depositor Collections Gene Vector Core Viral Vectors Gene Vector Core Viral...remove genes flanked by either loxP or FRT sites respectively, and empty vectors to express your gene of ...and puromycin resistance gene from the CMV promoter pCDH-CMV 72265 Express gene of interest from the CMV...pCDH-EF1 72266 Express gene of interest from the EF-1 promoter pCDH-CB 72267 Express gene of interest from ...promoter pCDH-EF1-copGFP-T2A-Puro 72263 Express copGFP and the puromycin resistance gene from the EF1 promoter...the cloning capacity. pCDH-CB-IRES-copGFP-T2A-Puro 72299 Express gene of interest from the CB promoter ... 72484 Express gene of interest from a truncated EF-1 promoter pCDH-EF1-Luc2-P2A-copGFP 72485 Expresses...

  7. Fluorescent Proteins: FRET

    Type
    Collection
    ...sREACh-C1 , mGFP-10-sREACh-N3 EGFP ShadowY** 488 0.6 531** 136,000 0.01 6.1 4.5 mEGFP-N1 , CMV-ShadowY , EGFP-ShadowY...(λ) Plasmids mTagBFP sfGFP 399 0.64 510 83,000 0.65 4.6 2.6 mTagBFP2-pBAD , sfGFP-pBAD ECFP EYFP 434 0.41...mTFP1-N1 , mCitrine-pBAD EGFP mCherry 488 0.60 610 72,000 0.22 5.3 1.9 mEGFP-N1 , mCherry2-N1 , pcDNA3.1...sReach-mTurquoise2 EGFP sREACh** (super-REACh, aka Nữ) 488 0.60 531** 115,000 0.07 6.0 4.1 mEGFP-N1 , sREACh-...Fixed Cells Additional Resources at Addgene Learn more on the Addgene Blog: Introduction to FRET and Tips...many FRET-based biosensors in Addgene's Biosensors Collection . Generate custom FRET-based biosensors ...pcDNA3.1 CMV mCherry-eGFP BGH Clover mRuby2 505 0.76 600 113,000 0.38 6.3 4.5 pcDNA3-Clover , pcDNA3-mRuby2...

  8. Arf GTPase Family

    Type
    Collection
    ...Plasmids by Gene The following table lists genes in the ARF GTPase family, information on each gene, and examples...each gene. Click the gene name for a full list of plasmids available. Please note that some genes may ...Plasmids by Gene The following table lists genes in the ARF GAP and GEF family, information on each gene, and...have plasmids available for request. Family Gene Entrez Gene ID Length (aa) Example Expression Systems ...EFA6) 5662 1024 Mammalian (pEGFP-C1) GEF Psd2 (EFA6C) 84249 771 Mammalian (pEGFP-C1), Gateway GEF Psd3 (EFA6D...

  9. AAV for Neuronal Tracing

    Type
    Collection
    ...G-deleted rabies lacks the viral glycoprotein (G) gene, which is not required for the transcription or .... Since this starter cell now has all the viral genes present, the virus can spread from this cell to ...Ready-to-use AAV available from Addgene's viral service encoding tools for monosynpatic retrograde neuronal...to perform retrograde neuronal tracing. Note : Addgene does not distribute rabies virus. AAV Vectors for...Description Serotype PI 52473 pAAV-synP-FLEX-splitTVA-EGFP-B19G Can be used to complement deletion-mutant rabies...AAV1 Ian Wickersham 100798 pAAV-syn-FLEX-splitTVA-EGFP-tTA These AAV together can be used to complement... was modified so that it could only infect a genetically specified neuronal population. This is done using...

  10. CRISPR Guide

    Type
    Collection
    ... editing multiple genes at once; using dual nickases to generate a knockout or gene edit; or using Cas9...the targeted gene. The ideal result is a loss-of-function mutation within the targeted gene. However, the...eliminate target gene function. It has also been used extensively to screen for novel genes that regulate... activate, or repress genes. Each library typically contains ∼3–6 gRNAs per gene to ensure modification...repress, visualize, and isolate genes. Activation or Repression of Target Genes Targeting dCas9 to transcription...single-gene activation (Figure 9D) Figure 9: Overview of CRISPRi/a. In bacteria, activating gene expression... of effective gene activators when fused with dCas9. Recently, synthetic CRISPR-Cas gene activators have...

  11. TALEN Engineering

    Type
    Collection
    ...Endogenous Zebrafish Genes TALENs for Endogenous Human Genes TALENs for the EGFP Reporter Gene Additional Plasmids...transcriptional activator target sites and then generates individual user-friendly graphical roadmaps for...

  12. Zebrafish Plasmid Collection

    Type
    Collection
    ...zebrafish gene of interest? Search Addgene's collection for plasmids that contain a zebrafish gene or sequence...zebrafish-specific gene expression database that provides information about when genes are active in developing...developing embryos. zfRegeneration - A database for gene expression profiling during regeneration. Seurat - R ...-type specific conditional gene inactivation - Jeffrey Essner Lab GeneWeld Vectors for Targeted Integration... finger nucleases, TALENS, and CRISPR/Cas9-based gene editing make the zebrafish a stalwart research model... model. With more than 70% of human disease genes having an ortholog in zebrafish, this species has more...kits are described below . ID Plasmid Description Gene/Insert PI Return to top Highlights of Zebrafish ...
Showing: 41 - 52 of 52 results