We narrowed to 112 results for: lars

...Cascade-Cas3 generates large genomic deletions. Insert CRISPR transposases insert large fragments of DNA. ...CRISPR experiment. CRISPR 101 eBook : Based on our popular CRISPR 101 blog series, we've organized a comprehensive...

...histidine-tagged constructs and routine protein and molecular biology techniques. Using a single protein with...species (e.g., human, watermelon, Streptomyces ) Subcellular compartment (chloroplastic, cytoplasmic, glyoxysomal...References for teachers Using Bioinformatics and Molecular Visualization to Develop Student Hypotheses in...

...GreenGate Cloning System (Plant) MoClo Modular Cloning Modular PUF Assembly Kit Optogenetics Pertz Lab...Sensing and Signaling Browse plasmids related to intercellular signaling and environmental sensing. Strains...

...Antibodies are large proteins made by the immune system that bind to an antigen, a particular part of a foreign...fragments that bind to the GCN4 peptide, from the popular SunTag system, fused to sfGFP for imaging. Learn...

...MoClo Parts Kit : Molecular cloning for E. coli - Densmore Lab EcoFlex MoClo : Modular cloning for applications...Find plasmids to perform your experiment in a particular bacterial expression system. Fluorescent Protein...

...to adult) and easy lab maintenance. Worms are particularly well suited for studies in cell biology, development...fluorescent proteins, and Cre expression plasmids. Large collections or kits are described below and not ...C. elegans strains. CRISPR plasmids from John Calarco's lab as described in Heritable genome editing in...

...target sites. (B) A TAL effector nuclease (TALEN). Similar in principle to the ZF nuclease, the components...sequence ( Figure 1A ). Scientists have identified a large number of zinc fingers that recognize various nucleotide...array is an effective targeting mechanism for molecular tools. ZFs are not without their limitations, ...

...Additional Resources The following tables list many popular fluorescent proteins and empty backbones for expression...complete collection and filter by vector type, popularity, and more. Blue/UV Protein Excitation (nm) Emission... to top Long Stokes Shift These proteins have a large gap between their excitation and emission maxima...time, allowing tracking of processes such as intracellular trafficking or cell cycle dynamics. Protein ...

... fusing EGFP-2A-PuroR cassette to a nucleolar protein, fibrillarin (FBL). To repeat the knock-in in your...Jorgensen Lab SapTrap CRISPR/Cas Toolkit SapTrap is a modular toolkit from Erik Jorgensen's lab for building ...

...Plasmid #188537) a better choice in place of the popular SepOTSλ (Plasmid #68292) . An important technical...translation system with enhanced biological tolerance . Molecular Systems Biology , 19 (8). https://doi.org/10.15252...recruitment and TBK1 activation to promote mitophagy . Molecular Cell , 60 (1), 7–20. https://doi.org/10.1016/j.molcel...

...selectable marker. They are frequently used in molecular biology to isolate, multiply, or express the insert... as GFP, which allows you to visualize the cellular localization of your protein. Additionally..., secretion, and display of proteins on the extracellular surface of S. cerevisiae cells His-tagged versions...

...Bidirectional DREADDs Activators Inhibitors Other Molecular Tools Clear Filters ID Name Promoter Activity ...simultaneous dTomato expression Recombinases Rylan Larsen 107788 AAV.rTH.PI.Cre.SV40 rTH Cre expression Recombinases...pAAV-FLEX-EGFPL10a EF1a EGFPL10a, Cre-dependent Molecular Tool Nathaniel Heintz , Alexander Nectow , Eric...

...OPEN method Modular Assembly Engineering Reagents - Engineer zinc finger arrays via Modular Assembly method...

...Mitogen-activated protein kinases (MAPKs), also known as extracellular signal-regulated kinases (ERKs), are serine/...threonine-specific protein kinases. They regulate diverse cellular programs by acting as an integration point for...

...disease in the world. Parasites such as Plasmodium (malaria), Toxoplasma (toxoplasmosis), Trypanosoma (African...Publication Return to top CRISPR Resources Addgene has a large selection of CRISPR plasmids and resources. Find...

...be used for screening transcription factors for cellular programming or cancer drug resistance....transcription factors for applications such as cellular programming or cancer drug resistance. Protocols...

...purification of any genomic sequence specified by a particular gRNA. In the enChIP (engineered DNA-binding molecule-mediated...Publication Return to top CRISPR Resources Addgene has a large selection of CRISPR plasmids and resources. Find...

...BRAIN Initiative supports the development of biomolecular tools for neuroscience research. Browse the ...development and application of innovative tools enabling large-scale real-time interrogation and monitoring of ...supports the development of a diverse portfolio of biomolecular tools and emphasizes their rapid and broad dissemination...

...ordering cloning grade DNA for over 200 of our most popular plasmids!... available in this format include a variety of popular plasmids and backbones with high cloning potential...

...produce antibodies are available as part of Addgene’s larger Antibody Plasmid Collection , which also includes... Tags and Other Markers : Antibodies targeting popular markers like tubulin or epitope tags like Myc, ...