Skip to main content
Addgene
Showing: 31 - 40 of 113 results

  1. AAV Packaged on Request

    Type
    Collection
    ...collection in the serotypes listed here. Details Scale and Titer Packaged on Request can be ordered in ...preparations, we set both minimum standards and typical titers, though actual yields may vary by construct...construct. Size Quantity Total Volume Minimum Titer Typical Titer Small 10 × 100 µL aliquots 1 mL 4 × 10 12... with a minimum titer of 2 × 10 12 GC/mL and a typical titer of 7 × 10 12 GC/mL. For more details, see...providing scientist through an MTA. This process typically takes 1–4 days, depending on your institution....

  2. CRISPR Plasmids - Bacteria

    Type
    Collection
    ...gRNA Design Tools CRISPR Blog Posts Base Edit Catalytically dead dCas9 fused to a cytidine deaminase protein...Gene/Insert Promoter PI Publication Activate Catalytically dead dCas9 fused to a transcriptional activator...Gene/Insert Promoter PI Publication Interfere Catalytically dead dCas9, or dCas9 fused to a transcriptional...cleaved the target RNA sequence, they adopt an enzymatically active state and can bind and cleave additional...ID Plasmid Promoter PI Publication Purify A catalytically inactive Cas9 (dCas9) can be used to purify ...

  3. Viral Vectors

    Type
    Collection
    ...are the most popular virus for gene transfer in clinical trials. These viruses have a relatively high transduction...Overview of Viral Plasmids While plasmids are typically used to encode, propagate, and manipulate genetic...as a safety feature, viruses used in research typically do not have the ability to propagate in the same...plasmids, however, retains the properties of any typical plasmid and thus can be easily modified and amplified... For example, adeno-associated virus (AAV) is typically preferred for in-vivo studies because of its low...

  4. Allen Institute for Brain Science AAV Enhancer Collection

    Type
    Collection
    ...for Brain Science BRAIN Armamentarium Collection Caltech Systemic Capsids AAV Packaged on Request AAV Guide...following regions: Isocortex Hippocampal formation Cortical subplate Striatum Pallidum Hypothalamus Cerebellum...pAAV-AiE2051m-minBG-SYFP2-WPRE3-BGHpA AiP1277 AiE2051m SYFP2 Claustrum Cortical subplate 220711 pAAV-AiE2051m-minBG-iCre(R297T...R297T)-BGHpA AiP1786 AiE2051m Cre Claustrum Cortical subplate 230518 pAAV-AiE2051m-minBG-FlpO-WPRE-HGHpA ...pAAV-AiE2051m-minBG-FlpO-WPRE-HGHpA AiP1211 AiE2051m FlpO Claustrum Cortical subplate 191720 pAAV-AiE0743m_3xC2-minBG-SYFP2-WPRE3-BGHpA ...and transgenic mouse lines for genetic access to cortical cell types. bioRxiv [Preprint]. 2024 Sep 26:2024.06.10.597244...

  5. Zebrafish Plasmid Collection

    Type
    Collection
    ...horizontal stripes on the sides of the adult body, is a tropical freshwater fish in the minnow family. It has long...inflammation and infection, regeneration, human neurological disorders, microbiota studies, and drug discovery...Cell lineage tracing in multicellular systems typically relies on genetic markers, somatic mutations, ...types. CreLite - George Eisenhoffer Lab. An optogenetically controlled Cre/loxP system that enables precise...assessment of lipoprotein abundance, size, and localization in high-throughput. MAZERATI platform - Leonard...

  6. Fujii Lab CRISPR Plasmids

    Type
    Collection
    ...consisting of a catalytically inactive form of Cas9 (dCas9) plus guide RNA (gRNA) for biochemical purification...analysis reveals genomic regulatory regions that physically interact with the Pax5 promoter in a chicken ...10.1093/dnares/dsx023. PubMed . Identification of physical interactions between genomic regions by enChIP-Seq...journal.pone.0123387. eCollection 2015. PubMed . A critical role of the Thy28-MYH9 axis in B cell-specific...

  7. Lentivirus Plasmids

    Type
    Collection
    ...divided among multiple plasmids. Some plasmids (typically called packaging and envelope plasmids) encode components...the plasmid that encodes the viral genome (typically called the transfer plasmid ). This table lists common...target cells. This genome is encoded by plasmids called "transfer plasmids," which can be modified to encode...

  8. Malate Dehydrogenase CUREs Community Collection

    Type
    Collection
    ...to mentors, virtual and local professional development, membership to a local hub of educators teaching...Hypotheses in a Malate Dehydrogenase Oriented CURE. Callahan KP et al. CourseSource. 2022 Jan 14. DOI: 10.24918...Learning Gains and Positive STEM Attitudes in CUREs. Callahan KP et al. CBE Life Sci Educ. 2022 Dec;21(4):ar74...

  9. EXtracellular Plasmid RESource (EXPRESs) Consortium

    Type
    Collection
    ...soluble recombinant molecules. The proteins are typically used in systematic protein interaction screens...Extracellular Interaction Screening (AVEXIS) for the scalable detection of low affinity extracellular receptor-ligand...Wright GJ. Genome biology 2009; 10(9):R99. Large-scale screening for novel low-affinity extracellular protein...Sun Y, Gallagher-Jones M, Barker C, Wright GJ. Analytical Biochem. 2012 May 1;424(1):45-53....

  10. Synthetic Biology - Overview

    Type
    Collection
    ...International Genetically Engineered Machine Foundation Registry of Standard Biological Parts - Repository...biology describes the rational engineering of biological systems for novel functions. Addgene is working...BioBricks BioBricks Foundation - Promoting open and ethical synthetic biology JBEI - Joint Bio-Energy Institute...
Showing: 31 - 40 of 113 results