We narrowed to 100 results for: MAL
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TypeCollection...Empty Backbones Genetic Code Expansion Immunology Mammalian RNAi Microbiology Plant Expression Stem Cells ... with the same backbone and only differing in a small region. Click here to view all pooled libraries....
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Bikard Lab - CRISPR Repression Collection
TypeCollection...bioRxiv preprint . Detailed information about chromosomal integration of pLC97 can be found in this article...Shearwin, K. E. (2013). One-Step cloning and chromosomal integration of DNA. ACS Synthetic Biology, 2(... -
Resolute Plasmid Collection
TypeCollection...academic research as well as applied research in small biotech and pharmaceutical companies. The RESOLUTE... fluorescence, mass/charge properties, protein thermal stabilization, and changes of voltage all contribute... -
Synthetic Biology - Overview
TypeCollection...Plasmids by Model Organism Bacterial Plant Yeast Mammalian Worm Fungal Algal Featured SynBio Deposits Alper...Balazsi Lab Negative Autoregulation and Yeast-Mammalian Circuit Transfer C. Collins Lab Quorum Sensing... -
Adenovirus Plasmids
TypeCollection...recombination of adenovirus plasmids in either mammalian cells or microorganisms, including bacteria and...This DNA molecule can then be transfected into mammalian packaging cell lines to generate adenovirus particles... -
Retrovirus Plasmids
TypeCollection...inducible expression of GFP T2A Cre fusion in mammalian cells Foijer 47916 pRXTN CMV/MSV pRXTN is a modified...-Luc MoMSV Stable expression of luciferase in mammalian cells Wong Return to Top Do you have suggestions... -
Antibody Production
TypeCollection...Production For recombinant antibodies (rAb) encoded by mammalian expression vectors, human embryonic kidney cells...target antigen is first transiently expressed in mammalian cells. To learn which specific QC experiments ... -
CRISPR Plasmids - Yeast
TypeCollection...Libraries gRNAs Empty gRNA Vectors gRNAs By Species Mammalian Bacteria Drosophila Plant C. elegans Yeast Zebrafish... C->T (or G->A on the opposite strand) within a small editing window specified by the gRNA. Adenine base... -
mTOR Pathway
TypeCollection...mTOR Gene List Resources The m echanistic or m ammalian t arget o f r apamycin (mTOR) is a key metabolic... enriched in brain S6K Also known as RPS6KB1; Ribosomal protein S6 kinase B1 TSC1/2 TSC1 TSC2 Tuberous... -
AAV Packaged on Request
TypeCollection...delivery tools that effectively transduce many mammalian cell types. AAV is used in a wide range of experiments...Quantity Total Volume Minimum Titer Typical Titer Small NEW 2 × 100 µL aliquots 0.2 mL 4 × 10 12 GC/mL* ... -
Adeno-associated virus (AAV) Plasmids
TypeCollection...AAV Plasmids Adeno-associated viruses (AAV) are small viruses originally discovered as contaminants of... The utility of AAV is currently limited by its small packaging capacity (∼4.5 kb including ITRs), though... -
CRISPR Plasmids - Bacteria
TypeCollection...Libraries gRNAs Empty gRNA Vectors gRNAs By Species Mammalian Bacteria Drosophila Plant C. elegans Yeast Zebrafish... C->T (or G->A on the opposite strand) within a small editing window specified by the gRNA. Adenine base... -
TALEN Guide
TypeCollection...Intuitively, one would expect that assembling relatively small, repeat-laden DNA regions into a single construct...sequence-specific TAL effectors for modulating mammalian transcription. Nat Biotechnol. 2011 Feb;29(2):... -
Viral Vectors
TypeCollection...into the host genome. AAVs are limited by their small packaging capacity. Adenovirus Plasmids | Guide ...delivery of genetic information to hard-to-transfect mammalian cells. In addition, "viral pseudotyping" can be... -
CRISPR Plasmids - Plants
TypeCollection...Libraries gRNAs Empty gRNA Vectors gRNAs By Species Mammalian Bacteria Drosophila Plant C. elegans Yeast Zebrafish... C->T (or G->A on the opposite strand) within a small editing window specified by the gRNA. Adenine base... -
Viral Production
TypeCollection...properties of the final product, particularly in large animal studies. Endotoxin contamination is minimized by...MRP 30 kDa subunit (RPP30) as a control for normalization purposes are being used. Quality Control Mycoplasma... -
Lentivirus Plasmids
TypeCollection...gene encoded by the transfer plasmid is toxic to mammalian cells, it can be difficult to efficiently produce...gateway destination plasmid for cDNA expression Ramalho-Santos 24129 pULTRA 3rd bi-cistronic expression... -
CRISPR Plasmids - Tagging
TypeCollection...Libraries gRNAs Empty gRNA Vectors gRNAs By Species Mammalian Bacteria Drosophila Plant C. elegans Yeast Zebrafish...tagging of proteins expressed from their natural chromosomal context. These CRISPR tagging methods allow for... -
Cre-Lox and Other Site-Specific Recombinases
TypeCollection...bp spacer (48 bp or 34 bp) F3, F5, FRT14, FRT15 minimal FRT GAAGTTCCTATTCtctagaaaGTATAGGAACTTC Table 1....improve expression. For example, iCre or FLPo in mammalian cells may facilitate higher rates of Cre-driven... -
Deisseroth INTRSECT Collection
TypeCollection...Flp, here expressed through a double transgenic animal strategy (PV-2a-Cre; SOM-IRES-Flp). Image from ...combinations of transgenic recombinase-expressing animal lines and retro-grade expressing viruses delivering...