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Addgene

Enter a sequence to perform a BLAST-based search of the Addgene plasmid sequence database.

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In Category

We narrowed to 126 results for: Ran

Showing: 61 - 80 of 126 results

  1. Distribution to Industry

    Type
    Collection
    ... Material Transfer Agreements Most Addgene orders must be accompanied by a material transfer agreement...industry scientists. Please note this does not guarantee availability but lets us know what plasmids would...agreement (MTA), including transfers to for-profit entities. The MTA is a legal agreement between the recipient... the depositing institution(s). It governs the transfer of biological materials and intellectual property...Please see the Sample Agreements on our Technology Transfer Information page for more information. To learn...

  2. CRISPR Pooled gRNA Libraries

    Type
    Collection
    ...Genome Editing Cut Base Edit Nick Prime Edit Transcriptional Regulation Activate Interfere Epigenetics RNA...Human Bonifacino 3rd 6-25 11,108 Borodina Yeast Transporter Libraries 153101-153106 Knockout Yeast Borodina...56,762 (Set A) 56,476 (Set B) Broad GPP activation Caprano p65-HSF 92383 (Set A) 92384 (Set B) Activation ...164084 Knockout Human Sabatini 3rd 10 7,501 Human Membrane Protein Activation Library 113345 Activation Human...Knockout Human Superti-Furga 3rd 6 2,609 Human Transporter Knockout Libraries 213695 221409 221410 Knockout...Human Superti-Furga 3rd Varies Varies Human Transcription Factor Knockout Library 162275 Knockout Human...Draetta 3rd 3-4 17,032 arrays Mouse Cardiac Transcriptional Regulators CRISPR Knockout Library 138015 Knockout...

  3. Rinehart Lab Phosphoprotein Reagents

    Type
    Collection
    ...optimization of an orthogonal translation system with enhanced biological tolerance. Mohler K, Moen JM, Rogulina...phosphorylation is one of the most abundant forms of posttranslational modifications in cells and research into its..., Science 2013) and improving the orthogonal translation system to make singly or multiply phosphorylated... ) and an optimized phosphoserine orthogonal translation system (pSerOTS-C1* (V70) 188537 ). These reagents... with the optimized phosphoserine orthogonal translation system ( 68292 ), they were able to demonstrate...

  4. Trimmer Lab NeuroMab Collection

    Type
    Collection
    ...easily and inexpensively generate R-mAbs by transiently transfecting the plasmids into mammalian tissue culture...leader sequence (L) for translocation of the heavy chain across the ER membrane. PCR-mediated fusion of...leader sequence (L) for translocation of the light chain across the ER membrane. Downstream of the insert... Mouse IgG2a 206524 Anti-SNAT1 transporter [N104/37R] SNAT1 transporter Rat Mouse IgG2a 206525 Anti-Ankyrin-G...high-throughput recombinant protein production by transient transfection of suspension-growing human 293-EBNA1 cells...generation R-mAbs plasmids, production of R-mAbs in transfected cells and their validation can be found below...heavy (blue) chains as driven by two CMV promoters (orange). Hybridoma-derived V L and V H domain PCR products...

  5. Neurodegeneration Research Collection

    Type
    Collection
    ...used to transform iPSCs and fibroblasts into neurons and more. New and Noteworthy: Study aberrant axon initial...associated with the disease and implicate a wide range of cellular processes. The vast majority of cases...kinetics and localization for imaging synaptic transmission. Aggarwal et al. Nat Methods. 2023 May 4. See...PiggyBac plasmids with tet-inducible expression of transcription factors for sensory, motor, and cortical neuron...Use a FRET-based method to monitor structural transitions in protein self-organization. Wan et al. Cell...

  6. CRISPR Plasmids - Repress Gene Expression

    Type
    Collection
    ...CRISPR plasmids for transcriptional repression of target genes....Genome Editing Cut Base Edit Nick Prime Edit Transcriptional Regulation Activate Interfere Epigenetics RNA...Catalytically dead dCas9, or dCas9 fused to a transcriptional repressor peptide like KRAB, can knock down...down gene expression by interfering with transcription. Design your gRNA to target your gene of interest...search the tables below for CRISPR plasmids for transcriptional inhibition. Plasmids are available for expression...

  7. AAV Viral Preps

    Type
    Collection
    ...tools that effectively transduce many mammalian cell types. AAV is used in a wide range of experiments, both... Packaged on Request service. You can choose a transfer plasmid from our repository and request packaging...Monster, Brainbow Molecular Tools Tetracycline Transactivators, Affinity Purification, Cell Ablation Monosynaptic...Testing Panel for the Eye and Brain For enhanced transduction of the eye and other tissues All Serotypes If...

  8. CRISPR Plasmids - Cascade-Cas3

    Type
    Collection
    ...non-targeted strand, Cas3 starts unraveling DNA using its helicase properties, cleaving the targeted strand non-specifically...Genome Editing Cut Base Edit Nick Prime Edit Transcriptional Regulation Activate Interfere Epigenetics RNA...After nicking the target DNA, Cas3 initiates single-strand DNA degradation. This degradation can continue ...non-specifically along the way. Cas3 initiates a final double-strand break, either due to a boundary defined by anti-CRISPR...

  9. Microbiology Resources

    Type
    Collection
    ...Addgene’s repository contains plasmid tools for a range of microbial fields, including bacteria, viruses...sp. Chlamydia sp. Clostridium sp. Deinococcus radiodurans Enterococcus sp. Geobacillus sp. Haemophilus ...Pertz Lab Bacterial Fluorescence Labeling : Broad-Range Rainbow Vectors - Barbier Lab Cloning and Expression... Integrative vector assembly of up to three transcription units in the oleaginous yeast Y. lipolytica ...Addgene depositors are making this possible in a range of organisms. Find CRISPR tools for use in genome...

  10. Worm Expression Resources

    Type
    Collection
    ...editing to easily engineer specific transgenic worm lines. The transparent body of the C. elegans hermaphrodite...Caenorhabditis elegans. Mos1 Based Transgenesis miniMos Transgenesis Kit - Erik Jorgensen Lab. This kit...kit contains 29 plasmids used to generate transgenic nematodes, including C. elegans and C. briggsae. Other...

  11. Lentiviral Prep Service

    Type
    Collection
    ... used to generate double-stranded breaks ( DSBs or cuts ) or single-stranded breaks ( nicks ). ID Name...Cas9 (dCas9) can be fused to a transactivator and used as a transcriptional activator . ID Name Insert Antibiotic... in a new window) . Please note this does not guarantee viral service, but lets us know what viruses would...

  12. Retrograde AAV viral preps

    Type
    Collection
    ...this serotype to deliver Cre-dependent transgenes in Cre transgenic lines. As part of our standard viral...serotype permits robust retrograde access and transgene delivery to projection neurons. Specific classes...titering by SYBR green qPCR , confirmation of the transfer plasmid with PCR, purity assessment by silver ... viral prep format. Please note this does not guarantee viral vector packaging service, but lets us know...

  13. CRISPR Plasmids - Empty gRNA Vectors

    Type
    Collection
    ...in vitro transcription BsaI none S. pyogenes Mark pU6T7 71462 Mammalian/in vitro transcription BsaI none...Genome Editing Cut Base Edit Nick Prime Edit Transcriptional Regulation Activate Interfere Epigenetics RNA...toolbox for multiplexed plant genome editing and transcriptional regulation Plant Three types of plasmids available...recombination. Qi Multiplex Conditional Mutagenesis Using Transgenic Expression of Cas9 and sgRNAs Zebrafish Plasmids...multiple functional gRNAs from a single precursor transcript Bullock pCRISPathBrick Bacteria Allows rapid ...

  14. CRISPR Plasmids - Activate Gene Expression

    Type
    Collection
    ...dead dCas9 fused to a transcriptional activator peptide can increase transcription of a specific gene. ...Genome Editing Cut Base Edit Nick Prime Edit Transcriptional Regulation Activate Interfere Epigenetics RNA...search the tables below for CRISPR plasmids for transcriptional activation. Plasmids are available for expression...

  15. CRISPR Plasmids - Zebrafish

    Type
    Collection
    ...Genome Editing Cut Base Edit Nick Prime Edit Transcriptional Regulation Activate Interfere Epigenetics RNA...functional CRISPR/Cas enzymes will introduce a double-strand break (DSB) at a specific location based on a gRNA-defined...PAM = NGG) 46759 pT7-gRNA T7 BsmBI In vitro transcription none, need Cas9 plasmid Chen and Wente 42250...42250 DR274 T7 BsaI In vitro transcription none, need Cas9 plasmid Joung Do you have suggestions for other...

  16. Optogenetics Plasmid Collection

    Type
    Collection
    ...plasmids encode for microbial opsins, which are membrane channels and pores derived from algae and archaebacteria...ChR2) from Chlamydomonas reinhardtii . ChR2 is a membrane channel that opens in response to blue light and...properties as well as improve the expression and membrane targeting in host cells. Other microbial opsins...cellular activity. For example, search "Cdc42", "transcription", "Hahn", or other terms. ID Plasmid Description...

  17. TALEN Engineering

    Type
    Collection
    ...Hockemeyer et al., Nat Biotechnol. 2011 ) TALE transcriptional activators in human cells ( Maeder et al., ...identify both dimeric TALEN and monomeric TALE transcriptional activator target sites and to simplify the ...sequences of interest for potential TALEN or TALE transcriptional activator target sites and then generates individual...Vectors for REAL, REAL-Fast and FLASH TALE Transcriptional Activator Expression Vectors for REAL, REAL-Fast...

  18. Antibody Plasmid Collection

    Type
    Collection
    ...mAb Collection . Once the plasmids are transiently transfected into mammalian tissue culture cells, the...making synthetic nanobodies (sybodies) against membrane protein targets. Synthetic single domain antibodies...antibodies for the conformational trapping of membrane proteins. Elife. 2018 May 24;7. and Generation of...

  19. mTOR Pathway

    Type
    Collection
    ...Loss of the tumor suppressor p53 also promotes aberrant mTORC1 activity, and multiple familial cancers... mTORC1 Complex Symbol Name 4E-BP Eukaryotic translation initiation factor 4E, binding protein 1 Cyclin...Cyclin D1 Also known as CCND1 eIF-4E Eukaryotic translation initiation factor 4E GBL/mLST8 MTOR associated....2014.01.001. PubMed PMID: 24508508 . Targeting the translation machinery in cancer. Bhat M, Robichaud N, Hulea...

  20. Adeno-associated virus (AAV) Plasmids

    Type
    Collection
    ...Addgene AAV transfer plasmids...or toxins should be handled at BSL-2. AAV can transduce both dividing and non-dividing cells with a low... AAV does not integrate into the host genome, transgene expression can be long-lived. The utility of AAV...viral genome (typically called cis -plasmid or transfer plasmid). Helper Plasmid ID Plasmid Serotype Description...
Showing: 61 - 80 of 126 results