...Information Featured Collections COVID-19 SARS-COV-2, ACE2, and CRISPR tools Fluorescent Proteins from ...Plasmid Gene/Insert PI Kits Kit name Type PI Description High Complexity Golden Gate Assembly Standards...Pooled Libraries Pooled Library name Type PI Description HR700_TP53 Exon Mutation Libraries CRISPR Thorsten...Parkinson's Disease; For target validation and drug design Antibodies Addgene distributes ready-to-use recombinant...materials and intellectual property rights, and provides liability protection for material providers. Addgene...

... and G-protein is delivered to the starter cell. (2) The starter cell now expresses TVA and G-protein....Vectors for Monosynaptic Neuronal Tracing ID Name Description Serotype PI 52473 pAAV-synP-FLEX-splitTVA-EGFP-B19G...

...transient mammalian expression 101126 pACP-tag (m)- 2 Vector ACP/MCP-tag Empty backbone for stable and transient...label of their choice according to the methods described by George et al. (2004). The ACP and SFP Synthases...respectively) may be expressed and purified as described previously in Stachelhaus et al. (1998) References...

...dual-transgenic recombinase-expressing mouse lines 2–9 and combinations of transgenic recombinase-expressing...delivering additional recombinases 10–14 . Figure 2: INTRSECT works by inserting short, intronic sequences... experimental design. How-to guide (Link opens in a new window) for the molecular design and testing of...interesting in implementing intersectional experimental design. The Deisseroth Lab maintains a Standard Operating...Gray Facilitate the Itch-Scratching Cycle via Descending Regulation. Neuron 101(1):45-59. PubMed (Link...

...for shipping stability by incubating at 37 °C for 2 weeks, freezing at -80 °C, thawing on ice, and then...protocols, titering methods, and quality control are described below. Information for a specific antibody can...

...Gordon Fishell 83899-AAV1 pAAV-mDlx-GCaMP6f-Fishell-2 GCaMP6f expression in forebrain GABA-ergic interneurons...Gordon Fishell 83899-AAV9 pAAV-mDlx-GCaMP6f-Fishell-2 GCaMP6f expression in forebrain GABA-ergic interneurons...Gordon Fishell 83899-AAVrg pAAV-mDlx-GCaMP6f-Fishell-2 GCaMP6f expression in forebrain GABA-ergic interneurons...brain cells and circuits during behavior. Long desired by researchers, the revolutionary dynamic picture... with Addgene materials. Plasmids ID Plasmid Description PI Viral Preps Addgene distributes ready-to-use...Learn more about our viral service ID Viral Prep Description PI 65417-AAV8 pAAV-hSyn-dF-HA-KORD-IRES-mCitrine...and proximal dendrites in a viral vector Hillel Adesnik 107708-AAV9 pAAV-hSynapsin- soCoChR-GFP A somatic...

...sequence YFP Thorben Dammeyer 54520 mCherry-Peroxisomes-2 Peroxisome Peroximal Targeting Signal 1 (PTS1) mCherry...Centrin2 Centrioles (dependent on cell cycle) Centrin-2 EGFP Erich Nigg 41151 pEGFP Cep170 C-term Centrioles...Robert Singer 27382 pDEST/N1-hEB1-GFP Microtubules EB1 GFP Robin Shaw 40908 pDEST/LIfeAct-mCherry-N1 Actin...Davidson 40907 pDEST/hCx43-EGFP-N1 Gap Junctions Connexin 43 EGFP Robin Shaw 49861 pDEST-mCherry-GJA1-20k-N1...encoding fluorescent proteins tagged with genes or peptides with known subcellular localization patterns. ...important to determine where your protein of interest resides. There are a variety of methods for determining...junctions, gap junctions, tight junctions, and desmosomes. Browse or search the table below to find markers...

...vectors designed for use with the Voytas Golden Gate TALEN Kit and are used in place of pTAL1, 2, 3, or...Sangamo)-Destination Pawel Pelczar pCAG-T7-TALEN(Sangamo)-Destination constructs were designed for optimal...pC-GoldyTALEN and RCIsricpt-GoldyTALEN are designed as destination vectors for the Voytas lab Golden Gate ...Plasmids pTAL5-BB and pTAL6-BB are designed as alternative destination vectors for the Golden Gate TALEN...TALEN(Sangamo)-FokI-KKR-Destination 40132 pCAG-T7-TALEN(Sangamo)-FokI-ELD-Destination Golden Gate TALEN 2.0...Mendenhall The TALE plasmid vectors described on this page are designed to target and edit the epigenome ...with custom repeat arrays. TALEN Kits TALEN Kit Description Depositing PI FusX TALEN Assembly System Contains...

...AAV2 vectors in the mouse retina. Mol Ther . Feb;19(2):293-301. PMID: 21045809 Other citations include: ...name for ordering information. ID Name Promoter Description Category PI 157970 pTR-UF11 chimeric CMV/Chicken...

...expression tTA Off Verma 26803 pEnt L1L3 EF1a-tTA-2 Contains Gateway L1L3 sites and EF1α promoter driving...tetracycline, tetracycline binds to tetR. This precludes tTA binding to the tetO sequences and subsequent...and induce your gene of interest. ID Plasmid Description Co-expressed tTA, rtTA, or TetR On or Off PI ...transactivator for your tetraycline system. ID Plasmid Description Element On or off PI 26429 pLenti CMV rtTA3 Blast...

...lab collection for more) pLexA and pACT2.2 - Yeast 2-hybrid plasmids Fly UAS, MT Targeted...Membrane Localization pWZL-Neo-Myr-Flag-DEST - pDest vector that adds a N-terminal myristoylation...expression pLenti CMV Neo DEST - Lentiviral Gateway destination vector for gene ...expression pLenti CMV Puro DEST - Lentiviral Gateway destination vector for gene ...expression pLenti CMV Hygro DEST - Lentiviral Gateway destination vector for gene ...Mammalian pLenti X1 GFP-Zeo DEST - Lentiviral Gateway destination for shRNA ...pLenti CMV/TO Zeo DEST - Tet-inducible lentiviral Gateway destination vector ...

...either (1) a lack of gRNA and/or Cas9 expression or (2) a lack of efficient target cleavage in cells expressing...more about how to design your gRNA . Browse Plasmids: gRNAs Synthesize and clone desired gRNAs Once you ...sequences it is time to design your gRNA oligos and clone these oligos into your desired vector. In many cases... Science Guides CRISPR Guide Plan Your Experiment Plan Your CRISPR Experiment Jump To: Plan Your Experiment...Select Genetic Manipulation Select Expression System Design gRNA Publications Get Started CRISPR is a powerful...available for your particular experiment. Select Your Desired Genetic Manipulation Different genetic manipulations...mammalian cell lines Select Your Target Sequence and Design Your gRNA Once you have selected your CRISPR components...

...extraction solution, runs the protocol, then uses around 2-4ul in each 50ul PCR reaction. The gDNA extracted ...more. CRISPR Design FAQs Homologous Recombination (HR) FAQs At the Bench FAQs CRISPR Design FAQs Should...template vector design guidelines? A few notes below are considerations for designing HR donor. Generally...usually pick 3-6 guides around the region to find the most efficient guide (most guides would work, but... this ‘split RNA’ design is not as efficient in genome editing as the second design (in pX330 / PX335 ...comparable efficiency to the optimized chimeric design but with better accuracy (in other words, lower...nickase system is based on the Cas9 D10A nickase described in Figure 4 of the Cong, et. al, 2013 Science ...

...- Bacterial Expression mScarlet-I3 568 592 68 4.2 2 min Monomer pmScarlet-I3_C1 - Mammalian Expression...Plasmids - Includes tagging with mCherry, mCitrine, mCerulean Davidson Lab Plasmids - Includes many N- and...fluorescence to obtain half-maximal value. Structure describes the monomeric or oligomeric state of the protein...System Plasmids Mammalian Golenbock Lab Plasmids - Includes tagging with CFP, YFP, and mRFP Tsoulfas Lab Lentiviral...Xenopus, and C. elegans Hamdoun Lab Plasmids - Set includes tagging with mCherry, Cerulean, Citrine, and eGFP.... elegans expression Yeast Thorn Lab Vectors - Includes tagging with a wide variety of colors, some specifically...yeast Lindquist Lab Vectors - Gateway cloning; includes tagging with ECFP, EYFP, DsRed, Cerulean Bacteria...