Skip to main content
This website uses cookies to ensure you get the best experience. By continuing to use this site, you agree to the use of cookies.

Please note: Your browser does not support the features used on Addgene's website. You may not be able to create an account or request plasmids through this website until you upgrade your browser. Learn more

Please note: Your browser does not fully support some of the features used on Addgene's website. If you run into any problems registering, depositing, or ordering please contact us at [email protected]. Learn more

Addgene
Showing: 61 - 76 of 76 results

  1. CRISPR Plasmids - Drosophila

    Type
    Collection
    ...Cas9 plasmid Bullock and Port pCFD2-dU6:2gRNA dU6:2 BbsI Injection or in vitro transcription Virmilion...Protocols gRNA Design Tools CRISPR Blog Posts The following CRISPR plasmids have been designed for use in ... to a genomic DNA locus and encodes a primer binding site and the desired edits on an RT template. Plasmid...CRISPR plasmids that have been designed for use in Drosophila and other insects. CRISPR... can increase transcription of a specific gene. Design your gRNA sequence to direct the dCas9-activator...

  2. Luciferase Plasmids

    Type
    Collection
    ...applications. LuxSit-i : An artificial luciferase desined de novo using deep-learning approaches. Split-Luciferase...fusing NanoLuc® and Venus fluorophore to Troponin C Ca 2+ binding domain. Nano-lanterns , Enhanced Nano-lanterns...transfection Feng Zhang 21471 pLenti PGK V5-LUC Neo (w623-2) Firefly PGK Lentiviral expression of firefly luciferase...to that of the fluorophore. Conformationally destabilized Firefly luciferase mutants : A set of three ...three sensors that are increasingly structurally destabilized versions of Firefly luciferase which serves ...dimerization , endosomal escape of cell-penetrating peptides , and more. nLuc/cLuc for split Firefly Luciferase...mammalian systems. ID Plasmid Luciferase Type(s) Description PI 16539 pBV-Luc Firefly Insertion of 5' promoter...

  3. Optogenetics AAV Preps

    Type
    Collection
    ...H134R)-EYFP CaMKII ChR2/H134R EYFP Constitutive 1, 2, 5, 9 Deisseroth 26973 pAAV-hSyn-hChR2(H134R)-EYFP....WPRE.SV40 CAG ChR2/H134R mCherry Constitutive 1, 2, 5, 9 Deisseroth 127090 pAAV-CAG-DIO-ChR2(H134R)-eYFP...ChrimsonR (soma-targeted) mRuby2 Cre dependent 9 Adesnik 124603 pAAV-EF1a-DIO-ChrimsonR-mRuby2-KV2.1-WPRE-SV40... ChroME (soma-targeted) mRuby3 Cre dependent 9 Adesnik 124651 pAAV-CamKIIa-ChrimsonR-mScarlet-KV2.1 CaMKII...eGtACR1 (soma-targeted) mRuby3 Cre dependent 9 Adesnik Inhibitory: iC++ 137155 pAAV-nEF-Con/Fon-iC++-EYFP...

  4. New England Biolabs Cell-Imaging Plasmid Collection

    Type
    Collection
    ...transient mammalian expression 101126 pACP-tag (m)- 2 Vector ACP/MCP-tag Empty backbone for stable and transient...label of their choice according to the methods described by George et al. 2004. The ACP and SFP Synthases...respectively) may be expressed and purified as described previously in Stachelhaus et al. 1998 References...

  5. Antibody Production

    Type
    Collection
    ...for shipping stability by incubating at 37 °C for 2 weeks, freezing at -80 °C, thawing on ice, and then...protocols, titering methods, and quality control are described below. Information for a specific antibody can...

  6. Brain Initiative Collection

    Type
    Collection
    ...Gordon Fishell 83899-AAV1 pAAV-mDlx-GCaMP6f-Fishell-2 GCaMP6f expression in forebrain GABA-ergic interneurons...Gordon Fishell 83899-AAV9 pAAV-mDlx-GCaMP6f-Fishell-2 GCaMP6f expression in forebrain GABA-ergic interneurons...Gordon Fishell 83899-AAVrg pAAV-mDlx-GCaMP6f-Fishell-2 GCaMP6f expression in forebrain GABA-ergic interneurons...brain cells and circuits during behavior. Long desired by researchers, the revolutionary dynamic picture... with Addgene materials. Plasmids ID Plasmid Description PI Viral Preps Addgene distributes ready-to-use...Learn more about our viral service ID Viral Prep Description PI 65417-AAV8 pAAV-hSyn-dF-HA-KORD-IRES-mCitrine...and proximal dendrites in a viral vector Hillel Adesnik 107708-AAV9 pAAV-hSynapsin- soCoChR-GFP A somatic...

  7. Cheng Lab CRISPR Casilio System

    Type
    Collection
    ...Wang W, Taghbalout A, Wang H. Cell Res. 2016 Feb;26(2):254-7. Open Access Link. PubMed PMID: 26768771 . ...also like... CRISPR Guide CRISPR Protocols gRNA Design Tools CRISPR Blog Posts Background Protocols and...resources and information. Researchers can obtain the desired modules to perform their functional genomic experiments...copies of Pumilio binding sites (PBS). ID Name Description 71888 pAC1371-pX-sgRNA-5xPBSa Cloning vector ...programmed to recognize a specific 8-mer PBS. ID Name Description 71881 pAC1355-pmax-NLSPUFa_VP64 NLSPUFa_VP64 ...

  8. Fluorescent Protein Guide: Subcellular Localization

    Type
    Collection
    ...sequence YFP Thorben Dammeyer 54520 mCherry-Peroxisomes-2 Peroxisome Peroximal Targeting Signal 1 (PTS1) mCherry...Centrin2 Centrioles (dependent on cell cycle) Centrin-2 EGFP Erich Nigg 41151 pEGFP Cep170 C-term Centrioles...Robert Singer 27382 pDEST/N1-hEB1-GFP Microtubules EB1 GFP Robin Shaw 40908 pDEST/LIfeAct-mCherry-N1 Actin...Davidson 40907 pDEST/hCx43-EGFP-N1 Gap Junctions Connexin 43 EGFP Robin Shaw 49861 pDEST-mCherry-GJA1-20k-N1...encoding fluorescent proteins tagged with genes or peptides with known subcellular localization patterns. ...important to determine where your protein of interest resides. There are a variety of methods for determining...junctions, gap junctions, tight junctions, and desmosomes. Browse or search the table below to find markers...

  10. TALEN Plasmids and Kits

    Type
    Collection
    ...vectors designed for use with the Voytas Golden Gate TALEN Kit and are used in place of pTAL1, 2, 3, or...Sangamo)-Destination Pawel Pelczar pCAG-T7-TALEN(Sangamo)-Destination constructs were designed for optimal...pC-GoldyTALEN and RCIsricpt-GoldyTALEN are designed as destination vectors for the Voytas lab Golden Gate ...Plasmids pTAL5-BB and pTAL6-BB are designed as alternative destination vectors for the Golden Gate TALEN...TALEN(Sangamo)-FokI-KKR-Destination 40132 pCAG-T7-TALEN(Sangamo)-FokI-ELD-Destination Golden Gate TALEN 2.0...Mendenhall The TALE plasmid vectors described on this page are designed to target and edit the epigenome ...with custom repeat arrays. TALEN Kits TALEN Kit Description Depositing PI FusX TALEN Assembly System Contains...

  11. Church Lab CRISPR Plasmids

    Type
    Collection
    ...to DNMT3b Yeast System: Table 2 To function in yeast cells, we designed Cas9 protein expression constructs...also like... CRISPR Guide CRISPR Protocols gRNA Design Tools CRISPR Blog Posts Bacteria and archaea have...can be accessed here . This set of plasmids is described in: RNA-Guided Human Genome Engineering via Cas9...can be ordered via the links below: ID Plasmid Description 41824 gRNA Empty Vector The backbone that a target...synthesis protocol 115.2 KB This set of plasmids is described in: Genome engineering in Saccharomyces cerevisiae...can be ordered via the links below: ID Plasmid Description 43802 p414-TEF1p-Cas9-CYC1t A human codon-optimized.... Our currently available set of Cas9 proteins includes three orthogonal variants optimized for use in...

  12. Tetracycline Inducible Expression

    Type
    Collection
    ...expression tTA Off Verma 26803 pEnt L1L3 EF1a-tTA-2 Contains Gateway L1L3 sites and EF1α promoter driving...tetracycline, tetracycline binds to tetR. This precludes tTA binding to the tetO sequences and subsequent...and induce your gene of interest. ID Plasmid Description Co-expressed tTA, rtTA, or TetR On or Off PI ...transactivator for your tetraycline system. ID Plasmid Description Element On or off PI 26429 pLenti CMV rtTA3 Blast...

  13. Empty Backbones - Choosing Your Perfect Plasmid Backbone

    Type
    Collection
    ...lab collection for more) pLexA and pACT2.2 - Yeast 2-hybrid plasmids Fly UAS, MT Targeted...Membrane Localization pWZL-Neo-Myr-Flag-DEST - pDest vector that adds a N-terminal myristoylation...expression pLenti CMV Neo DEST - Lentiviral Gateway destination vector for gene ...expression pLenti CMV Puro DEST - Lentiviral Gateway destination vector for gene ...expression pLenti CMV Hygro DEST - Lentiviral Gateway destination vector for gene ...expression pLenti X1 GFP-Zeo DEST - Lentiviral Gateway destination for shRNA ...pLenti CMV/TO Zeo DEST - Tet-inducible lentiviral Gateway destination vector ...

  14. Zhang Lab's CRISPR Frequently Asked Questions

    Type
    Collection
    ...extraction solution, runs the protocol, then uses around 2-4ul in each 50ul PCR reaction. The gDNA extracted ...more. CRISPR Design FAQs Homologous Recombination (HR) FAQs At the Bench FAQs CRISPR Design FAQs Should...template vector design guidelines? A few notes below are considerations for designing HR donor. Generally...usually pick 3-6 guides around the region to find the most efficient guide (most guides would work, but... this ‘split RNA’ design is not as efficient in genome editing as the second design (in pX330 / PX335 ...comparable efficiency to the optimized chimeric design but with better accuracy (in other words, lower...nickase system is based on the Cas9 D10A nickase described in Figure 4 of the Cong, et. al, 2013 Science ...

  15. Deisseroth INTRSECT Collection

    Type
    Collection
    ...dual-transgenic recombinase-expressing mouse lines 2-9 and combinations of transgenic recombinase-expressing...consider as part of experimental design. How-to guide for the molecular design and testing of novel INTSRECT...interesting in implementing intersectional experimental design. The Deisseroth Lab maintains a Standard Operating...Gray Facilitate the Itch-Scratching Cycle via Descending Regulation. Neuron 101(1):45-59. PMID:30554781...

  16. Fluorescent Protein Guide: Empty Backbones

    Type
    Collection
    ...- Bacterial Expression mScarlet-I3 568 592 68 4.2 2 min Monomer pmScarlet-I3_C1 - Mammalian Expression...Plasmids - Includes tagging with mCherry, mCitrine, mCerulean Davidson Lab Plasmids - Includes many N- and...fluorescence to obtain half-maximal value. Structure describes the monomeric or oligomeric state of the protein...System Plasmids Mammalian Golenbock Lab Plasmids - Includes tagging with CFP, YFP, and mRFP Tsoulfas Lab Lentiviral...Xenopus, and C. elegans Hamdoun Lab Plasmids - Set includes tagging with mCherry, Cerulean, Citrine, and eGFP.... elegans expression Yeast Thorn Lab Vectors - Includes tagging with a wide variety of colors, some specifically...yeast Lindquist Lab Vectors - Gateway cloning; includes tagging with ECFP, EYFP, DsRed, Cerulean Bacteria...
Showing: 61 - 76 of 76 results