...optimization of an orthogonal translation system with enhanced biological tolerance. Mohler K, Moen JM, Rogulina...phosphorylation is one of the most abundant forms of posttranslational modifications in cells and research into its..., Science 2013) and improving the orthogonal translation system to make singly or multiply phosphorylated... ) and an optimized phosphoserine orthogonal translation system (pSerOTS-C1* (V70) 188537 ). These reagents... with the optimized phosphoserine orthogonal translation system ( 68292 ), they were able to demonstrate...

...CRISPR plasmids for transcriptional repression of target genes....Genome Editing Cut Base Edit Nick Prime Edit Transcriptional Regulation Activate Interfere Epigenetics RNA...Catalytically dead dCas9, or dCas9 fused to a transcriptional repressor peptide like KRAB, can knock down...down gene expression by interfering with transcription. Design your gRNA to target your gene of interest...search the tables below for CRISPR plasmids for transcriptional inhibition. Plasmids are available for expression...

... epitope tags either alone or fused to the transcriptional activator VP64. When directed to its target...target in gene promoters by the sgRNA expressed in trans from pSPgRNA, the dCas9-VP64 fusion protein activates... used to inhibited gene expression via steric hindrance. These plasmids are described in: RNA-guided gene... gene activation by CRISPR-Cas9-based transcription factors. Perez-Pinera P, Kocak DD, Vockley CM, Adler... pyogenes dCas9 (D10A, H840A) fused to VP64 transactivator domain in mammalian cells. 47108 pSPgRNA : ...

...generate single-strand DNA breaks. Prime Edit Cas nickase enzymes fused to reverse transcriptase install new...Engineering Cut Wild-type Cas enzymes generate double strand breaks (DSBs). Base Edit Base editors precisely...genetic information into a specific DNA site Transcriptional Regulation Screen Activate dCas9 fused to an..., or fused to a repressor peptide, inhibits transcription. Epigenetics dCas9 fused to an epigenetic modifier...

...Addgene AAV transfer plasmids...or toxins should be handled at BSL-2. AAV can transduce both dividing and non-dividing cells with a low... AAV does not integrate into the host genome, transgene expression can be long-lived. The utility of AAV...viral genome (typically called cis -plasmid or transfer plasmid). Helper Plasmid ID Plasmid Serotype Description... tTA-TRE amplification system for mouse lung transduction after intravenous injection Gradinaru 196679...-PR rep/cap AAV-PR encodes AAV-PR capsid that transduces pericytes and smooth muscle cells in mice after...

...Addgene’s repository contains plasmid tools for a range of microbial fields, including bacteria, viruses...sp. Chlamydia sp. Clostridium sp. Deinococcus radiodurans Enterococcus sp. Geobacillus sp. Haemophilus ...Pertz Lab Bacterial Fluorescence Labeling : Broad-Range Rainbow Vectors - Barbier Lab Cloning and Expression... Integrative vector assembly of up to three transcription units in the oleaginous yeast Y. lipolytica ...Addgene depositors are making this possible in a range of organisms. Find CRISPR tools for use in genome...

...editing to easily engineer specific transgenic worm lines. The transparent body of the C. elegans hermaphrodite...Caenorhabditis elegans. Mos1 Based Transgenesis miniMos Transgenesis Kit - Erik Jorgensen Lab. This kit...kit contains 29 plasmids used to generate transgenic nematodes, including C. elegans and C. briggsae. Other...

...this serotype to deliver Cre-dependent transgenes in Cre transgenic lines. As part of our standard viral...serotype permits robust retrograde access and transgene delivery to projection neurons. Specific classes...titering by SYBR green qPCR , confirmation of the transfer plasmid with PCR, purity assessment by silver ...available in viral format. Please note this does not guarantee viral service, but lets us know what viruses would...

...dead dCas9 fused to a transcriptional activator peptide can increase transcription of a specific gene. ...Genome Editing Cut Base Edit Nick Prime Edit Transcriptional Regulation Activate Interfere Epigenetics RNA...search the tables below for CRISPR plasmids for transcriptional activation. Plasmids are available for expression...

...vitro transcription none, need Cas9 plasmid Chen and Wente DR274 T7 BsaI In vitro transcription none, ...Genome Editing Cut Base Edit Nick Prime Edit Transcriptional Regulation Activate Interfere Epigenetics RNA...functional CRISPR/Cas enzymes will introduce a double-strand break (DSB) at a specific location based on a gRNA-defined...

...Monster Brainbow Molecular Tools Tetracycline Transactivators Affinity Purification Cell Ablation Monosynaptic...Testing Panel for the Eye and Brain for enhanced transduction of the eye and other tissues All Serotypes If... The entire Penn Vector Core catalog has been transfered to Addgene and available for distribution. All...Service Suggestion Form . Please note this does not guarantee viral service, but lets us know what viruses would...

...plasmids encode for microbial opsins, which are membrane channels and pores derived from algae and archaebacteria...ChR2) from Chlamydomonas reinhardtii . ChR2 is a membrane channel that opens in response to blue light and...properties as well as improve the expression and membrane targeting in host cells. Other microbial opsins...cellular activity. For example, search "Cdc42", "transcription", "Hahn", or other terms. ID Plasmid Description...

...Hockemeyer et al., Nat Biotechnol. 2011) TALE transcriptional activators in human cells (Maeder et al., Nat...identify both dimeric TALEN and monomeric TALE transcriptional activator target sites and to simplify the ...sequences of interest for potential TALEN or TALE transcriptional activator target sites and then generates individual...Vectors for REAL, REAL-Fast and FLASH TALE Transcriptional Activator Expression Vectors for REAL, REAL-Fast...

...in vitro transcription BsaI none S. pyogenes Mark pU6T7 71462 Mammalian/in vitro transcription BsaI none...Genome Editing Cut Base Edit Nick Prime Edit Transcriptional Regulation Activate Interfere Epigenetics RNA...toolbox for multiplexed plant genome editing and transcriptional regulation Plant Three types of plasmids available...recombination. Qi Multiplex Conditional Mutagenesis Using Transgenic Expression of Cas9 and sgRNAs Zebrafish Plasmids...multiple functional gRNAs from a single precursor transcript Bullock pCRISPathBrick Bacteria Allows rapid ...

...mAb Collection . Once the plasmids are transiently transfected into mammalian tissue culture cells, the...making synthetic nanobodies (sybodies) against membrane protein targets. Synthetic single domain antibodies...antibodies for the conformational trapping of membrane proteins. Elife. 2018 May 24;7. and Generation of...

...Loss of the tumor suppressor p53 also promotes aberrant mTORC1 activity, and multiple familial cancers... mTORC1 Complex Symbol Name 4E-BP Eukaryotic translation initiation factor 4E, binding protein 1 Cyclin...Cyclin D1 Also known as CCND1 eIF-4E Eukaryotic translation initiation factor 4E GBL/mLST8 MTOR associated....2014.01.001. PubMed PMID: 24508508 . Targeting the translation machinery in cancer. Bhat M, Robichaud N, Hulea...

...includes listing of transfer status for viral preps.... Viral Service Penn Vector Core Transfer Viral Service:...Service: Penn Vector Core Transfer The University of Pennsylvania Vector Core (Link opens in a new window) (...inventory , which includes all viral vectors transferred from the Penn Vector Core. Chemogenetics (including...available in viral format. Please note this does not guarantee viral service, but lets us know what viruses would...

...plasmids with dCas9 fused to FokI for creating double-strand breaks (DSBs)....Genome Editing Cut Base Edit Nick Prime Edit Transcriptional Regulation Activate Interfere Epigenetics RNA...nuclease. When FokI dimerizes, it generates a double-strand break (DSB) at a specific sequence. Two unique ...

...controlling the number of mismatches we can obtain a range of expression levels. This collection comprises ...Together, these plasmids allow to explore the whole range of expression levels for these two reporters simultaneously...Teeffelen S. Tuning dCas9’s ability to block transcription enables robust, noiseless knockdown of bacterial...

...kinase that anchors a signaling network for transitioning between anabolic and catabolic states. Ras Ras...frequently mutated gene in human cancer, is a transcription factor and tumor suppressor. Wnt Coming soon...Tool for Lineage Tracing: The ClonTracer Library (Frank Stegmeier) Tackling Cancers’ Drug Resistance with...