We narrowed to 140 results for: TOR;

... within that gene. To construct ZFN expression vectors, DNA encoding zinc finger arrays should be cloned...plasmids also available through Addgene--the specific vector to use depends on the length of the spacer sequence...between the half-sites (see Nuclease Expression Vectors for more details). IMPORTANT NOTE: All zinc finger...tCCCATCCTAtcgttGCATGAGCTc mdka (midkine-related growth factor) OZ527 and OZ528 OPEN OPEN cACCCTCCTCgtggtGCTGGTGGCc...aAGCTTCCTCttcattgGCTGACTGCa ldr (low density lipoprotein receptor) OZ539 and OZ540 OPEN OPEN tTGCATCCACggcagTCGGCAGTGc...OPEN OPEN gAGCTCCGTCtcgctgGCGGCCGAAg histamine receptor H3 (hrh3) OZ549 and OZ550 OPEN OPEN gCGCCACGGAcaataaaGAGGACTGCa...

...signed by an authorized signatory at the recipient institution. An authorized signatory is someone who can ...also adding additional filters such as expression, vector type, species, and more. Plasmids Browse the table...Golden Gate Assembly Standards Kit Cloning Eric Cantor Kit for using comprehensive end-joining ligation...Libraries CRISPR Thorsten Stiewe Use these donor vector libraries for CRISPR/Cas9-mediated saturation genome...entity is qualified to receive materials from our repository. All purchases are subject to Addgene's Terms...

...General Tools Fire Lab C. elegans Vector Kit - Andrew Fire Lab. A set of vectors for C. elegans research, including...plasmid tools for the community that aren't in the repository? Help us widen our resources for the community...plasmids that streamlines the production of targeting vectors for tag insertion using CRISPR/Cas9 and the selection... approach has also been developed by the Fire laboratory and described in Efficient Marker-Free Recovery...including lacZ and GFP fusion vectors. C. elegans optimized fluorophores - Andrew Fire Lab. Available ...

...References The ARF (ADP-ribosylation factor) family of regulatory GTPases have long been characterized...exchange factors), and downstream (by GAPs, GTPase activating proteins, which also serve effector roles)...ARF family plasmids. ARF family members include regulatory GTPases, GEFs, and GAPs. Plasmid..., 2016). Though best known for their roles as regulators of membrane traffic, they also play critical ...

...Examples Include: promoters and terminators repressors and activators logic gates Networks and Gene Regulation...plasmids available from this depositor's lab. Plasmid Gene/Insert Vector Type Promoter PI Publication ...includes both naturally ocurring and synthetic regulatory elements, pre-assembled genetic circuits, and...

...plasmids to study the regulatory networks involved in retinal development.... Depositor Collections Brzezinski...mouse retina. To aide in their studies of the regulatory networks involved in retinal development the ...Prdm1 and Vsx2 enhancers in the retina alters photoreceptor and bipolar cell fate specification, yet differs...

... SLCs in Gateway-compatible pENTR vectors (pDONR221). These vectors allow a fast and efficient sub-cloning...framework that provides biological context. Assays monitoring impedance, fluorescence, mass/charge properties... into a wide range of Gateway-compatible pDEST vectors. ID Plasmid Gene/Insert Industry PI Pooled Libraries...the Resolute Partnership using their RESOLUTE repository feedback form (Link opens in a new window) . ...

... Depositor Collections FreeGenes Project Open Enzyme Collection... own enzymes by cloning them into an expression vector of their choice and transforming into E. coli bacteria...Researchers should clone these enzymes into an expression vector of their choice and then transform into E. coli...expression and purification ( pET28a-LIC or other pET vectors are recommended). The selection marker for all ... top Additional Resources Bacterial Expression Vectors Synthetic Biology Plasmids MoClo Guide Drew Endy...

... Depositor Collections Rinehart Lab Phosphoprotein Reagents...Library #111704) is anticipated to be useful for laboratories performing phosphoproteomic experiments in need...can also be introduced into a second expression vector encoding a split mCherry fluorescent reporter, ...domain and the phosphosite, mCherry fluorescence is restored, so interactions can be discovered by fluorescence-activated... via partial UAG codon reassignment and release factor 1 deletion . FEBS Letters , 586 (20), 3716–3722...

... Viral Vector Packaging Service AAV Biosensors Viral Vector Packaging Service: Biosensor...CaMKII Dlx EF1a GFAP/GfaABC1D minBG Synapsin E2 regulatory element Activity Cre-dependent Flp-dependent ...Karpova and Schaffer labs. See the Retrograde AAV Inventory Page . Don’t See What You’re Looking For? Our ... eligible AAV plasmids and we’ll make the viral vector prep you need. Or fill out our form to let us know...format. Please note this does not guarantee viral vector packaging service, but lets us know what viruses...

... Depositor Collections Cell Migration Consortium (CMC) Plasmids...Cell Migration Consortium and Addgene. Member laboratories of the Cell Migration Consortium have deposited...for cell migration research at Addgene. CMC investigators who have deposited plasmids include: Joan Brugge...Francisco Browse Plasmids ID Plasmid Gene/Insert Vector Type Backbone Mutations Tags PI...

... plasmid tools generated by IGVF Consortium investigators to examine how genomic variation affects genome... Depositor Collections IGVF Consortium Impact of Genomic Variation...collaborative program that brings together teams of investigators who will use state-of-the-art experimental and...plasmid tools through Addgene. ID Plasmid Description Vector Type PI Publication Additional Resources The IGVF...

...will aid neuroscientists in studying circuits in laboratory animals and human tissue specimens. These technologies...rAAV-hI56i-minBglobin-iCre-4X2C-WPRE3-BGHpA AAV vector for Cre expression in forebrain GABAergic interneurons...lines or co-infection with Cre dependent viral vectors. Jonathan Ting Viviana Gradinaru 163509-PHPeB CN1839...CN1839-rAAV-hSyn1-SYFP2-10aa-H2B-WPRE3-BGHpA AAV vector for nuclear SYFP2 labeling in neurons. Useful for...BGHpA DLX2.0 (3xCore version of DLX enhancer). AAV vector for strong & rapid SYFP2 expression in forebrain...

...gRNA sequence to direct the dCas9-activator to promoter or regulatory regions of your gene of interest....Catalytically dead dCas9 fused to a transcriptional activator peptide can increase transcription of a specific...separate gRNA expression plasmid to target the dCas9-activator to your specific locus. Figure 1: Overview of ...

...any viral vector. Addgene performs QC measures which verify the identity of the viral vector, but not ... Viral Vector Packaging Service Packaged on Request Scope of Service AAV Packaged on Request: Scope of...guarantees above. We will have our expert viral vector production team carefully review your cargo/serotype...

...antibodies (rAb) encoded by mammalian expression vectors, human embryonic kidney cells are transfected with...Neuromab Protocols (Link opens in a new window) page. Storage and Shipping Stability Addgene does not recommend... suggests preparing single-use aliquots before storing at -20 °C. Each antibody catalog item is tested...some of our other resources to help you choose, store, or use your antibody. Antibody Blog Posts Recipient...

...Modular Assembly method Nuclease Expression Vectors - Cloning vectors for expressing zinc finger FokI nuclease...proteins for distribution to academic and nonprofit laboratories for research and educational use. Links to additional...

...are expressed and purified from plasmids in our repository....summarize antibody production and storage techniques, and explain common applications. View...some of our other resources to help you choose, store, or use your antibody. Antibody Blog Posts Recipient...

...Fluorescent Proteins: Plasmids and Resources Addgene's repository contains thousands of fluorescent protein plasmids...conformational changes within a protein. Biosensors Monitor small biomolecules or other physiological intracellular...small molecules to activate or inhibit engineered receptors that affect cell signalling pathways. Luciferase...

...fragments of DNA at specific locations. The Cas effector lacks nuclease activity, instead recruiting the... and type V-K. Type I systems use a type I Cas effector, composed of several separate Cas enzymes that...target site. Type V-K systems use a type V-K Cas effector, Cas12k, and three transposase components (TnsB...