...transfer plasmids in our repository are eligible for this service. Select any eligible plasmid in the Addgene...applicable transfer plasmid. We will help you gain legal usage of the transfer plasmid from the providing...real-world troubleshooting. Eligible Plasmids The following plasmids are eligible for this service. Submit...paper of your chosen AAV plasmid for more information on its performance. ID Plasmid Gene/Insert Promoter ...Overview Details Ordering Share Your Results Eligible Plasmids Additional Resources Adeno-associated virus (AAV...lets scientists choose from over 3,000 transfer plasmids to be packaged in their choice of five serotypes...2.5 mL, or 5 mL). Currently, we can only package plasmids in our collection in the serotypes listed here...

...CRISPR Plasmids: CRISPR Transposases (CASTs) Browse CRISPR Plasmids By Function Genome Editing Cut Base...search the tables below for CRISPR Transposase plasmids. Plasmids are available for expression in mammalian...Mammalian ID Plasmid Gene/Insert Promoter Selectable Marker PI Publication Bacteria ID Plasmid Gene/Insert...suggestions for other plasmids that should be added to this list? Fill out our Suggest a Plasmid form or e-mail...Find plasmids for CRISPR-associated transposases (CASTs) for insertion of donor DNA. CRISPR...

... CRISPR Epigenetics CRISPR Plasmids: Epigenetics Browse CRISPR Plasmids By Function Genome Editing Cut...CRISPR plasmids for epigenetic modification in mammalian or plant systems. Mammalian ID Plasmid Gene/Insert...suggestions for other plasmids that should be added to this list? Fill out our Suggest a Plasmid form or e-mail...CRISPR plasmids for targeted epigenetic modification, including histone acetylation/demethylation, and...Promoter Selectable Marker PI Publication Plant ID Plasmid Gene/Insert Promoter Selectable Marker PI Publication...

...the tables below for CRISPR prime editing plasmids. Plasmids are available for expression in mammalian...Mammalian ID Plasmid Gene/Insert Promoter Selectable Marker PI Publication Bacteria ID Plasmid Gene/Insert...Plant ID Plasmid Gene/Insert Promoter Selectable Marker PI Publication Drosophila ID Plasmid Gene/Insert...selectable marker, and whether the plasmid contains Cas9. ID Plasmid Expression System Promoter Guide RNA...suggestions for other plasmids that should be added to this list? Fill out our Suggest a Plasmid form or e-mail...prime editor plasmids for (search and replace) genome editing. CRISPR... CRISPR Prime Edit CRISPR Plasmids: Prime Edit...

...select CRISPR lentiviral plasmids. CRISPR Plasmid Kits : Find groups of plasmids that are used together ...resources or browse plasmids below by function. Addgene's CRISPR collections feature plasmids for a variety ...CRISPR plasmids available at Addgene. Search by species and CRISPR function. CRISPR... CRISPR Plasmids and Resources...inhibition, and barcoding experiments. Browse CRISPR Plasmids by Function Genome Engineering Cut Wild-type Cas...experiment based on a variety of factors. gRNAs gRNA plasmids to target specific genes....

...Table 2. pmax dCas9 Activator Expression Plasmids ID Plasmid 48223 pAC91-pmax-dCas9VP64 : dCas9VP64 on...CRISPR Cas9 gRNA plasmids from Dr. Rudolf Jaenisch's lab. CRISPR... CRISPR Jaenisch Lab CRISPR Plasmids Jaenisch Lab...Lab CRISPR Plasmids Available from Addgene You may also like... CRISPR Guide CRISPR Protocols gRNA Design...different systems by LR clonase reaction. These plasmids are described in: Multiplexed activation of endogenous...Link opens in a new window) Article . Individual plasmids can be ordered via the links below: Table 1. dCas9... dCas9 Activators sgRNA Dual Expression ID Plasmid 48236 pAC2-dual-dCas9VP48-sgExpression : Dual expression...

... Consortium Plasmids Browse SGC Oxford Collection Structural Genomics Consortium Plasmids Structural Genomics... Genomics Consortium Plasmids - Oxford Plasmid Gene/Insert Vector Type Backbone Mutations Tags PI...Browse plasmids from the Structural Genomics Consortium (SGC) Oxford collection Plasmid... Plasmid Collections Structural...

...Consortium Plasmids Browse SGC Stockholm Collection Structural Genomics Consortium Plasmids Structural...Structural Genomics Consortium Plasmids - Stockholm Plasmid Gene/Insert Vector Type Mutations Tags Industry PI...Browse plasmids from the Structural Genomics Consortium (SGC) Stockholm collection Plasmid... Plasmid Collections...

... Consortium Plasmids Browse SGC Toronto collection Structural Genomics Consortium Plasmids Structural ...Structural Genomics Consortium Plasmids - Toronto Plasmid Gene/Insert Vector Type Backbone Mutations Tags Industry...Browse plasmids from the Structural Genomics Consortium (SGC) Toronto collection Plasmid... Plasmid Collections ...

... Plasmid Collections Mammalian RNAi Mammalian shRNA Tools for RNAi Browse Plasmids Resources ...BioRender.com. shRNA Plasmids Browse our selection of empty vectors for cloning in shRNAs. Most plasmids are in a ...Plasmids for mammalian RNA interference (RNAi) experiments at Addgene; browse by constitutive lentiviral... occur via transfection or viral delivery of a plasmid. shRNAs are often delivered using viral vectors...with some retroviral and AAV backbone options. Plasmids for constitutive expression as well as those that...inducible (Tet) expression are available. To find plasmids containing other RNAi components, like argonaute...argonaute, please search our full site . ID Plasmid Vector Type PI Publication Additional Resources Addgene...

...phenotyping, and distribution efforts. Plasmids The following plasmids can be used to create cell lines with...Find plasmids generated by the iPSC Neurodegenerative Disease Initiative (iNDI) here. Depositor...Also Like... CRISPR Guide CRISPR Protein Tagging Plasmids for Stem Cell Research Neurodegeneration Research... with the research community. To this end, all plasmids that result from the project efforts will be made...made available for research purposes in the iNDI plasmid collection at Addgene. Figure 1: Schematic of iNDI... cell types, as noted in the purpose field: ID Plasmid Description Additional Resources iNDI (Link opens...

...like... Keith Joung Lab plasmids Daniel Voytas Lab plasmids Scot Wolfe Lab plasmids Detailed Information ... room temperature. Individual plasmids can be ordered from each plasmid page and will be shipped as bacterial...Depositing Labs: Keith Joung This kit consists of plasmids and strains used to engineer ZFNs via the Oligomerized.... This kit consists of 5 individually packaged plasmids and 3 individually packaged strains, which are...Nature Protocols paper . How to Cite this Kit These plasmids were created by your colleagues. Please acknowledge.... For your Materials and Methods section: "The plasmid kit used in this publication was a gift from Keith...

...BioBricks Foundation Plasmids Drew Endy Lab Plasmids Jennifer Molloy Lab Plasmids Golden Gate cloning ...Golden Gate assembly. Plasmids Explore the collection and order individual plasmids via the links below:...Browse the Open Enzyme collection of plasmids at Addgene for generating enzymes for molecular biology... (Link opens in a new window) . How to Use The plasmids in the Open Enzyme collection contain genetic ...are recommended). The selection marker for all plasmids in this collection is ampicillin. Find more information...Polymerases Other Enzymes DNA Ligases Addgene ID Plasmid Gene/insert 165503 pOpen-HSlig4 DNA Ligase IV 165514...Ligase III Return to top DNA Polymerases Addgene ID Plasmid Gene/insert 165500 pOpen-9N7polA 9 degrees N-7 ...

...Institute for Cell Science Plasmid Collection Allen Institute for Cell Science Plasmid Collection You May Also...full description of how to use these plasmids, check out Plasmids for Endogenous Gene Tagging in Human...Allen Institute for Cell Science has deposited plasmids to generate fluorescently tagged proteins of key...Fluorescent Protein Guide Subcellular Localization Plasmids Protocols The Allen Institute for Cell Science...induced pluripotent stem cell (hiPSC) lines. The plasmids used to generate these lines have been deposited...distribution to the research community. With these plasmids researchers will be able to generate fluorescently...Human Cells on the Addgene Blog. Plasmids for Endogenous Gene Tagging in Human Cells Find additional cell...

...scheme Fujii Lab Plasmids Individual plasmids can be ordered via the links below: ID Plasmid Description 47948...elements/pMD20 iChIP plasmid, LexA-binding elements 48874 3xFNLDD/pCMV-7.1 iChIP plasmid, expresses 3xFNLDD...-I2 iChIP plasmid, expresses 3xFNLDD in mammalian cells 48972 3xFNLDD/pMXs-IG iChIP plasmid, expresses... gRNA plasmids from Dr. Hodaka Fujii's lab. Genome... Genome Engineering CRISPR Fujii Lab CRISPR Plasmids Fujii ...Fujii Lab CRISPR Plasmids Available from Addgene You may also like... CRISPR Guide CRISPR Protocols gRNA Design...information and protocols, check our references for the plasmids or browse the Fujii Lab web page . Figure 1: iChIP...

... like... Keith Joung Lab plasmids Daniel Voytas Lab plasmids CRISPR plasmids The Joung Lab has developed...Gene Additional Plasmids compatible with the REAL Assembly TALEN Kit: TALE-LSD1 plasmids for epigenome ... simplify the construction of TALEN expression plasmids using REAL, REAL-Fast or FLASH assembly. This ...REAL or REAL-Fast assembly process or lists of plasmid ID numbers required for FLASH assembly. Links to...Assembly TALEN Kit Individual REAL TALE Repeat Plasmids TALEN Expression Vectors for REAL, REAL-Fast and...

...the right to use the original plasmid. Distribution of the original plasmid is important because it is the...Overview of Addgene's viral preparation service for plasmids and pooled libraries. Viral preparation options...produces high-quality viral vector preps from select plasmids in our collection. We know that viral vector production... production is a common step after requesting plasmids from Addgene, and by providing this service, we...options. Select from a vast catalog of eligible AAV plasmids and we’ll make the viral vector prep you need....a sample of the purified DNA from the original plasmid that was used to make the virus. In order for Addgene...inclusion is what gives you permission to use the plasmid....

...to view all plasmids available from the article. Click on the PI link to view all plasmids available from...suggestions for other plasmids that should be added to this list? Fill out our Suggest a Plasmid form or e-mail... Plasmid Collections Synthetic Biology Bacterial Strains Synthetic... from this depositor's lab. Plasmid Description Gene/Insert Antibiotic Resistance PI Publication Back ...

... and plasmid collections that may help with your neurodegeneration research. Disease Info Plasmid Collection...Research Tools Neurodegeneration Plasmid Collection A collection of plasmids expressing wild type or mutant...Subcellular Localization, Optogenetics Plasmids, and Chemogenetics Plasmids. New and Noteworthy: Explore NeuroPAL...more. Plasmids provided by the foundation can be found on our Michael J. Fox Foundation Plasmid Resource...Find out about the plasmid collections available at Addgene that may be useful for neurodegeneration ...genes linked to neurodegenerative disorders. Find plasmids expressing HTT, C9ORF72, LRRK2, Synuclein, and... species. You can also find our guide to using plasmid pooled libraries . New and Noteworthy: Use a CRISPRi...

...below for CRISPR plasmids for RNA editing in mammalian systems. Mammalian ID Plasmid Gene/Insert PI Publication...suggestions for other plasmids that should be added to this list? Fill out our Suggest a Plasmid form or e-mail...CRISPR plasmids based on Cas13 designed to precisely edit RNA targets. CRISPR...Plasmids: RNA Editing Browse CRISPR Plasmids By Function Genome Editing Cut Base Edit Nick Prime Edit Transcriptional...