We narrowed to 13 results for: AAV Cas9

... PubMed . Return to AAV In vivo plasmids AAV Plasmids with Cas9 Mouse CRISPR-Cas9 knockin mice for genome... Blog Posts Jump to: SpCas9 Plasmids GeCKO SAM AAV - In vivo Delivery AAV - Cas9 Mouse CRISPR ( C lustered...sorting of cells and nuclei. Return to top AAV - Cas9 mouse CRISPR-Cas9 is a versatile genome editing technology...constitutively Cas9-expressing mouse. Described here are AAV vectors that can be combined with Cas9 in a wide... al., Nature Protocols 2013). SpCas9 Cas9: The PX551 plasmid (AAV-SpCas9, #60957) contains a single cassette...Return to top Cas9 SAM transcriptional activation plasmids and screening library CRISPR/Cas9 Synergistic...that allow the delivery of Cas9 and sgRNA in vivo via adeno-associated virus (AAV). The single vector system...

...). You will decide how to express Cas9, the delivery system for Cas9 and the guide RNA (gRNA), the design...non-homologous end joining (NHEJ) at the cut site Cas9 or Cas9 nickase High-fidelity Cas enzymes increase specificity...insertions, by copying from a DNA donor template Cas9 or Cas9 fusions with proteins that promote HDR Requires...site. Consider alternative Cas9 enzymes with different PAM sequences or Cas9 enzymes with flexible PAM ... collection of in-stock lentiviral preps . AAV vectors AAV vectors offer some advantages for viral vector...delivery. Both lentiviral and AAV vectors are safe to use in the lab, but AAVs exhibits the lowest immunogenicity... transgene expression with AAVs can be long-lived, despite recombinant AAVs not integrating into the host...

...Genome Engineering Tools These AAV encode Cas9 nuclease used in the CRISPR-Cas9 system for targeted genome...codon-optimized Cas9 from Staphylococcus aureus (SaCas9). 6, 8 Feng Zhang Labeling Tools These AAV encode tools... System Activity Serotype PI 61592 pX600-AAV-CMV::NLS-SaCas9-NLS-3xHA-bGHpA CMV-driven, constitutive Expression...Ready-to-use AAV available from Addgene's viral service encoding tet-off transactivators and tools for... Viral Vector Packaging Service AAV Molecular Tools Viral Vector Packaging Service: Molecular...Tetracycline Transactivators and Inducible Tools These AAV encode tetracycline-inducible tools/controls and ... Gradinaru Tools for Affinity Purification These AAV encode tools for affinity purification (which can...

...High fidelity Cas9s: Three high fidelity Cas9 variants , SpCas9-HF, eSpCas9, and HypaCas9 display very ...Staphylococcus aureus (SaCas9) is smaller than SpCas9 and more easily packaged in AAV. Cas9 orthologs also have...III and Cas9 enzymes, which together separate the individual crRNAs. (3) The tracrRNA and Cas9 nuclease...beyond SpCas9, moving us closer to being able to target every locus in every genome. Multiple Cas9 orthologs...capability of Cas9 to make other types of modifications at specific loci. Activate or Repress : dCas9 fused to...the crRNA would bind. (5) After the complex binds, Cas9 separates the double stranded DNA target and cleaves...Single multi-domain effector) Type I (Cas3) Type II (Cas9) Type III (Cas10) Type V (Cpf1) Type IV (Csf1) Type...

...contains Cas9. ID Plasmid Expression System Promoter Guide RNA Type Cloning Enzyme Co-expressed Cas9 Selection...transcriptase (RT) is fused to the C terminus of Cas9 H840A nickase. The fusion enzyme is capable of installing...template. During prime editing, the pegRNA directs the Cas9 nickase to the target sequence where it nicks the...nuclear localization signals, and two mutations in Cas9 to enhance nuclease activity; can be used in PE2...PE6 — small prime editors with optimized RT and/or Cas9 domains PE7 — addition of RNA-binding exonuclease...PuroTK-pegRNA_GG Mammalian, piggyBac hU6 pegRNA BsmBI Yes (Cas9 H840A + MMLV RT) PuroTK Jacob Giehm Mikkelsen 174038... RNA-H1-nick sgRNA-mCherry Mammalian, AAV hU6 + H1 pegRNA + nicking sgRNA No mCherry Hyongbum...

... available with multiple Cas9 variants, including high fidelity Cas9s, Cas9s with various PAM requirements...strand SpCas9-HF1 — disrupt Cas9’s interactions with DNA phosphate backbone HypaCas9 — increase Cas9 proofreading...more Cas9 fusion tools later in the guide. Figure 3: Overview of Cas9n. Increasing fidelity of Cas9 Researchers...enhanced specificity by engineering Cas9 into high fidelity Cas9s (hfCas9) . There is currently no defined...application. Cas9 Orthologs While SpCas9 is the most commonly used Cas enzyme, additional Cas9 orthologs ...constructs with Cas9 fused to proteins that modulate HDR or NHEJ components, like miCas9 , Cas9-DN1S , or others...Plasmids: Purify Cas9 Alternatives for CRISPR Genome Engineering S. pyogenes Cas9 (SpCas9) is the most commonly...

...protein production AAV-GfaABC1D-MCS--4x6T-WPRE - For astrocyte-selective expression in AAV Dual promoter Separate...Advantages Representative Empty Backbones AAV High transduction efficiency, but do NOT integrate...expression, see our dedicated Adeno-associated Virus (AAV) Plasmids page Lentiviral Can transduce both dividing... Separate expression pRGEB31 - Deliver sgRNA and Cas9 into plants by Agrobacterium-mediated transformation...Express sgRNA/PTG with rice snoRNA U3 promoter and Cas9 with rice ubiquitin promoter for Agrobacterium-mediated... FP Collection NLS Nuclear localization dCas9 plasmid - dCas9 expression plasmid without effector fusions...

...about how to minimize leakiness in experiments using AAV vectors. Highlighted Plasmids Browse the tables below...preps feature Cre-dependent tools, or consider our AAV Packaged on Request service for more options. Addgene...Activity with GFP Plasmids 101: FLEx Vectors Optimizing AAV DIO and FLEx Vector Expression Plasmids 101: Knockout...preps feature Flp-dependent tools, or consider our AAV Packaged on Request service for more options. Addgene...CRISPR Knockout Library CRISPR Sidi Chen Use this AAV CRISPR knockout library expressing luciferase and...vivo using a mouse line expressing Cre-dependent Cas9. Kits Kit Name Type PI Description dRMCE Plasmid...Return to top Related Addgene Plasmid Collections AAV Enhancer Collection Fluorescent Biosensors Chemogenetics...

...Carrier Proteins (SLCs) from the RESOLUTE collection AAV Packaging from Addgene Parkinson's Research from ...Stiewe Use these donor vector libraries for CRISPR/Cas9-mediated saturation genome editing of human TP53...

...APEX targeting Alice Ting 85040 pK170.AAV-TRE-Cre-WPRE (Supernova) AAV vector for dox-inducible expression...sgRNA TetR H1-2O2 Nathanael Gray 35625 pAAV-Ptet-RFP-shR-rtTA AAV Tet-On shRNA vector. To evaluate shRNA...promoter Tet-On 3G rtTA David Vereide 120309 pAAV-FAH-rtTA3G AAV vector to express Tet-On 3G transactivator...promoter TetR Eric Campeau , Paul Kaufman 175274 pAAV-rtTA AAV Tet-On vector with neuron-specific expression...Plasmid #27106 for rtTA. tTA Edward Hsiao 99118 pAAV-CAG-tTA AAV Tet-Off vector, expresses tTA from the CAG... Tet-Off tTA-Advanced Takeshi Imai 104109 pAAV-Syn1-tTA AAV Tet-Off vector, expressed tTA from the hSyn...tet-inducible Cas9-2A-EGFP expression. Based on LentiCRISPR v2 . Adam Karpf 167935 pLenti-tetON-KRAB-dCas9-DHFR-EF1a-TagRFP...

...pX330S-2-PITCh - expresses Cas9 & PITCh-gRNA pX330A-FBL/PITCh - expresses Cas9, PITCh-gRNA, & FBL-specific...#79888) , which expresses an untagged Cas9 AND a gRNA targeting the AAVS1 locus. Doyon Tagging Plasmid: 3xFLAG... consists of two components: A vector containing Cas9 and a validated gRNA, based on the Zhang lab's PX458...need to design and clone in a gRNA(s) to guide the Cas9 protein to your target sequence, as well as design...consists of two components: An all-in-one CRISPR/Cas9 vector based on the Zhang lab's PX330 , simultaneously... to generate both an expression cassette for the Cas9-programming sgRNA and HR donors for selectable genome...for integrating at the AAVS1 "safe harbor" locus: eSpCas9(1.1)_No_FLAG_AAVS1_T2 Doyon Lab TAP Tagging ...

... N. (2017). Systematic gene tagging using CRISPR/Cas9 in human stem cells to illuminate cell organization...Tight junction protein ZO-1 Tight junctions 91565 AAVS1-mEGFP AICSDP-35 mEGFP NA Cytoplasm 101781 CETN2-...mEGFP Ras-related protein Rab-5A Endosomes 107580 AAVS1-mTagRFPT-CAAX AICSDP-42 mTagRFPT CAAX domain of ...AICSDP-29 mTagRFPT Lamin B1 Nuclear envelope 114404 AAVS1-mEGFP (PGK) AICSDP-36 mEGFP NA Cytoplasm 114405 ...

...sgHTT51-7sk-Cas9 HTT U6, 7sk Huntington's Nicole Deglon 190902 pAAV2ss-EFS-GFP-synPolyA-U6-sgHTT1 HTT GFP U6...-FP Plasmid Type Mammalian, non-viral Lentiviral AAV Retroviral Bacterial Yeast Other Promoter CMV T7 ... ALS, motor neuron disease Trina Schroer 75437 p-AAV-sh[SNCA] SNCA U6 Parkinson's Edward Burton 75637 ...Parkinson's Frank Soldner 180437 Px330-EGFP-LRRK2-CRISPR/Cas9 LRRK2 U6 Parkinson's Frank Soldner 180439 STR-KDEL_PGRN-SBP-mCherry...Hart 190900 pAAV2ss-U6-sgHTT1-7sk-sgCas9 HTT U6, 7sk Huntington's Nicole Deglon 190901 pAAVss-U6-sgHTT51...Parkinson's Hilal Lashuel 36055 pAAV asyn WT SNCA CMV Parkinson's Hilal Lashuel 36056 pAAV asyn S87A SNCA CMV Parkinson's...Hilal Lashuel 36066 pAAV asyn S129A SNCA CMV Parkinson's Patrick Aebischer 36067 pAAV asyn S129G SNCA CMV...