Genetic Code Expansion
Type
Collection
...substrate for endogenous aaRS enzymes to load standard amino acids. tRNA and aaRS pairs from Methanocaldococcus...pairs of aaRS and tRNA that work for one organism may not work for another. The orthogonal aaRS enzyme ...protein translation, an aminoacyl-tRNA synthetase (aaRS) enzyme loads a specific amino acid to its tRNA....engineered to recognize the reassigned codon. An aaRS enzyme is engineered to specifically load the new... the ncAA. Together, the engineered tRNA and the aaRS enzyme form an orthogonal pair, which means they...cell's natural genetic machinery. The orthogonal aaRS enzyme should not recognize endogenous tRNAs, and...plasmid expressing the engineered tRNA and its cognate aaRS enzyme evolved to incorporate ncAAs. This plasmid...