Skip to main content
Addgene
Open Menu

Enter a sequence to perform a BLAST-based search of Addgene plasmid sequence databases.

Information

In Category

We narrowed to 19 results for: aare

Clear keywords
Showing: 1 - 19 of 19 results

  1. Hot Plasmids and Viral Preps - May 2021

    Type
    Blog Post
    ...antibodies targeting fourteen aminoacyl tRNA synthetases (aaRS) have been created, characterized, and validated... Figure 2: Specific binding of scFVs to cognate aaRS antigens as measured by suspension bead assay. Image... the Gräslund lab. Recent findings suggest that aaRSs are involved in inflammation and autoimmune diseases...antibody tools could be used to study the role of aaRSs in these processes. To create the antibody toolbox...would be in their native conformation. Because many aaRSs exist within a multi-tRNA synthetase complex, the...whether the generated antibodies could bind these aaRSs. They found the antibodies were able to immunoprecipitate...

  2. Genetic Code Expansion

    Type
    Collection
    ...substrate for endogenous aaRS enzymes to load standard amino acids. tRNA and aaRS pairs from Methanocaldococcus...pairs of aaRS and tRNA that work for one organism may not work for another. The orthogonal aaRS enzyme ...protein translation, an aminoacyl-tRNA synthetase (aaRS) enzyme loads a specific amino acid to its tRNA....engineered to recognize the reassigned codon. An aaRS enzyme is engineered to specifically load the new... the ncAA. Together, the engineered tRNA and the aaRS enzyme form an orthogonal pair, which means they...cell's natural genetic machinery. The orthogonal aaRS enzyme should not recognize endogenous tRNAs, and...plasmid expressing the engineered tRNA and its cognate aaRS enzyme evolved to incorporate ncAAs. This plasmid...

  4. Donations from Addgene to Yield Answers for Rare Disease Researchers

    Type
    Blog Post
    ...in the symptoms experienced by AHC patients. Waardenburg Syndrome Last but not least, Meng-Shin Shiao...and colleagues will put their award to work on Waardenburg syndrome, a rare disease that can cause hearing...diagnosed at their hospital with one form of Waardenburg syndrome (WS4) by Pediatrician Duangrurdee Wattanasirichaigoon...

  5. Uncovering Ancestral Bias in CRISPR Research

    Type
    Blog Post
    ...accessible to the research community. Sean Misek and Aaron Fultineer in the Beroukhim and Boehm labs pulled...either European or East Asian ancestry, Sean and Aaron knew that their study had significant statistical...varying burden across ancestries. Sean A Misek, Aaron Fultineer, Jeremie Kalfon, Javad Noorbakhsh, Isabella...

  13. Neurodegeneration Plasmid Collection

    Type
    Collection
    ...TARDBP YFP GAL1 ALS Aaron Gitler 27448 pRS416 Gal G294A YFP TARDBP YFP GAL1 ALS Aaron Gitler 27449 pRS416...TARDBP YFP GAL1 ALS Aaron Gitler 27450 pRS416 Gal Q331K YFP TARDBP YFP GAL1 ALS Aaron Gitler 27451 pCold...Factor Xa cspA ALS Aaron Gitler 27452 pCold TDP43 G294A TARDBP His, Factor Xa cspA ALS Aaron Gitler 27453 pCold...Factor Xa cspA ALS Aaron Gitler 27454 pCold TDP43 Q331K TARDBP His, Factor Xa cspA ALS Aaron Gitler 27458 pRS416...WT TARDBP GAL1 ALS Aaron Gitler 27459 pRS416 Gal TDP43 G294A TARDBP GAL1 ALS Aaron Gitler 27460 pRS416...M337V TARDBP GAL1 ALS Aaron Gitler 27461 pRS416 Gal TDP43 Q331K TARDBP GAL1 ALS Aaron Gitler 27462 pDuet ...WT TARDBP GST GAL ALS Aaron Gitler 27463 pDuet TDP-43 G294A TARDBP GAL ALS Aaron Gitler 27464 pDuet TDP...

  14. Fluorescent Protein Guide: Biosensors

    Type
    Collection
    ... 2+ Sensor. PLoS One. 2013 Dec 2;8(12):e82009. Maarten Merkx Potassium Green (GINKO) and raitometric (...homeostasis. Nat Methods. 2009 Oct;6(10):737-40. Maarten Merkx Zinc eZinCh-2 Zn 2+ FRET sensor, cytosolic...Imaging. ACS Chem Biol. 2015 Sep 18;10(9):2126-34. Maarten Merkx Zinc Cytosolic Cyan-Yellow Zinc FRET sensors...

  15. Control AAV Preps

    Type
    Collection
    ...pAAV-EF1a-mitoGFP-DIO EF1a mito-GFP Cre dependent 2 Maarten Kole 190155 AAV_pMBP-EGFP-caax MBP EGFP-caax Constitutive...

  18. Biosensor AAV Preps

    Type
    Collection
    ...4mtGCaMP6f-DIO EF1a 4mtGCaMP6f none Cre dependent 1 Maarten Kole Calcium Sensor: GCaMP6m 100838 pAAV.Syn.Flex.GCaMP6m.WPRE.SV40...
Showing: 1 - 19 of 19 results