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We narrowed to 16 results for: c myc expression vector

Showing: 1 - 16 of 16 results

  1. Plasmids for Stem Cell Research

    Type
    Collection
    ...polycistronic lentiviral vector for the expression of human Oct4, Klf4, Sox2, and c-Myc Human Induced Pluripotent...doxycycline-inducible lentiviral vector for the expression of human Oct4, Klf4, c-Myc, and Sox2 Integrative Analyses...doxycycline-inducible lentiviral vectors for the expression of mouse Oct4, Klf4, c-Myc, and Sox2 Reprogramming ...doxycycline-inducible lentiviral vectors for the expression of mouse Oct4, Klf4, c-Myc, and Sox2 Excluding Oct4...lentiviral vector for the expression of human Oct4, Sox2, and Klf4 Polycistronic lentiviral vector for "hit...Human Doxycycline-inducible expression of human Oct4, Sox2, Klf4, and c-Myc from four separate lentiviral...-24. Mikkelsen Lentivirus Human Expression of human Klf4, Oct4, c-Myc, and Sox2 as VP-16 transcriptional...

  2. Empty Backbones - Choosing Your Perfect Plasmid Backbone

    Type
    Collection
    ...tag pKMyc - N-terminal Myc tag for mammalian expression pGEX-4T-1-3xMyc - Bacterial vector for Myc tag ...compatible Y2H vector - BD pRPR1_gRNA_handle_RPR1t - gRNA expression empty vector pFA6a vectors - Epitope ...lentiviral vector for ORF expression pDest-565 - Gateway destination vector for bacterial expression; His,GST...gene expression vector URA3 Yeast pAG306GAL-ccdB - Yeast Gateway expression vector (see...Inducible Expression page Bacteria Lac, T7, araBAD, trp mTagBFP2-pBAD - Protein expression vector with C-terminal...surface display vector with a C-terminal Myc tag pPMW-attB - pUASp plasmid with N-terminal Myc tag and attB...mammalian expression pENTR4-myc-nuc - pEntry vector that adds a C-terminal nuclear localization signal...

  3. Luciferase Plasmid Collection

    Type
    Collection
    ...Multi-luciferase reporter vector including five transcriptional reporters for NF-kb, TGF-b, c-Myc, p53, and MAPK/...cells Peter Cockerill 212936 pGL3 Basic Vector Firefly Vector for investigating regions controlling transcription...a reference level of expression. Browse or search for popular plasmids expressing luciferase. We also ...Mammalian expression of Nano-lantern Takeharu Nagai 87121 pcDNA-RLuc8 Renilla CMV Mammalian expression Renilla...CMV AAV expression of firefly luciferase and GFP James Wilson 22522 phGluc Gaussia EF1α Expression of Gaussia...Firefly TRE Lentiviral vector with dox- or tet-inducible luciferase expression Stephen Tapscott 83281 ....1 NL NanoLuc® CMV Mammalian expression of Nanoluc with a N-terminal Myc tag Erich Wanker 124701 pLenti-PGK-Venus-Akaluc...

  4. Tetracycline Inducible Expression

    Type
    Collection
    ...popular empty backbones, expression vectors for transactivators, and vectors for other applications, or...Lentiviral Tet-Off vector for repressible expression with blasticidin selection. *Note: this vector contains an...PB-EF1a-TetOn3G PiggyBac vector expressing Tet-On 3G transactivator constitutively expressed by EF1a promoter ...120309 pAAV-FAH-rtTA3G AAV vector to express Tet-On 3G transactivator under the c-Fos promoter Tet-On 3G ...Lentiviral Tet-On vector for inducible expression; insert with Gateway cloning and puromycin selection rtTA-Advanced...Tetracycline (Tet) Inducible Expression Tetracycline (Tet) Inducible Expression You may also like... Blog...factors to further minimize background expression. Improved expression of the transactivator proteins in mammalian...

  5. Bacterial Expression Systems

    Type
    Collection
    ... Plasmid Collections Bacterial Expression Systems Bacterial Expression Systems Protein Purification Protein...bacterial expression systems. Read below and browse our curated collection of bacterial expression plasmids...Addgene Blog E. Coli Strains for Protein Expression Protein Expression Epitope Tags Protein Tags Return to ...to top Controlled Expression It isn’t always advantageous to constitutively express your gene of interest...turn on expression only at a specific time. This collection contains plasmids whose expression levels ...peptides include: Epitope tags: 6xHis, Flag, Strep II, c-Myc, HA, V5, GST Solubility tags: MBP, SUMO, TrxA, Mocr...Temperature (42 °C) Escherichia coli Yingfeng An Other Addgene Controlled Expression Resources Check out...

  6. CRISPR Plasmids - Empty gRNA Vectors

    Type
    Collection
    ... Empty Vectors Many gRNA empty vectors have been deposited at Addgene. To find the gRNA vector for your...constructing all-in-one expression vectors containing multiple guide RNA expression cassettes and a Cas9 ...size and allow for expression of more sgRNAs in a single vector. sgRNAs are expressed as polycistronic ...gRNA expression vectors for insertion of custom gRNA target sequences. CRISPR...CRISPR Plasmids: Empty gRNA Expression Vectors Browse CRISPR Plasmids By Function Genome Editing Cut Base...by the Doudna Lab . Plasmid Expression System Cloning Enzyme Co-expressed Cas9 Cas9 System Selection PI...BbsI none S. pyogenes Gersbach pUC57-sgRNA expression vector 51132 Mammalian BsaI none S. pyogenes Huang...

  7. Viral Production

    Type
    Collection
    ... with viral vector manufacturing facilities, such as the University of Pennsylvania Vector Core. Transfections...produced the viral vector lot. Contact us to determine which facility produced your viral vector lot. At Addgene...Transducibility Some viral vectors are tested in vitro and in vivo for gene expression and/or function. These...Empty vector particles can be identified after negative staining and appear darker than full vector particles...Electron micrograph of AAV vector preparation shows that the vast majority of the vectors consist of full particles...-80 °C. Titer Titering is either performed by Addgene or by the University of Pennsylvania Vector Core...80 °C. Titer All titering is performed on lentiviral preparations that have been stored at -80 °C and ...

  8. CRISPR Plasmids - Bacteria

    Type
    Collection
    ...j.bbrc.2013.08.013. Empty gRNA Expression Vectors Select a gRNA expression plasmid based on factors such...choose does not also express a gRNA, you will need to use a separate gRNA expression plasmid to target the...using does not also express a gRNA, you will need to use a separate gRNA expression plasmid to target the...Libraries gRNAs Empty gRNA Vectors gRNAs By Species Mammalian Bacteria Drosophila Plant C. elegans Yeast Zebrafish...repressor peptide like KRAB, can knock down gene expression by interfering with transcription. Design your...cloning method. When using CRISPR, you will need to express both a Cas protein and a target-specific gRNA in...both the gRNA and Cas protein act as all-in-one vectors, but their function is often limited to a single...

  9. Cre-Lox and Other Site-Specific Recombinases

    Type
    Collection
    ... plasmids expressing Cre recombinase and filter the results by expression system and vector type. Find... GFP Plasmids 101: FLEx Vectors Optimizing AAV DIO and FLEx Vector Expression Plasmids 101: Knockout/Knock-In... plasmids expressing Flp recombinase and filter the results by expression system and vector type. Find... plasmids expressing Dre recombinase and filter the results by expression system and vector type. Find...genome or control gene expression or skip ahead to browse highlighted plasmids expressing Cre , Flp , Dre ,...Recognition Sites. Controlling Recombinase Expression By expressing the enzyme at specific times or locations...popular recombinase-dependent gene expression strategies. Gene Expression: A stop codon flanked with loxP...

  10. CRISPR Plasmids - Plants

    Type
    Collection
    ...Marker PI Publication Empty gRNA Expression Vectors Select a gRNA expression plasmid based on factors such...choose does not also express a gRNA, you will need to use a separate gRNA expression plasmid to target the...using does not also express a gRNA, you will need to use a separate gRNA expression plasmid to target the...Libraries gRNAs Empty gRNA Vectors gRNAs By Species Mammalian Bacteria Drosophila Plant C. elegans Yeast Zebrafish...CRISPR expression plasmids that have been designed for use in plants. CRISPR...repressor peptide like KRAB, can knock down gene expression by interfering with transcription. Design your...cloning method. When using CRISPR, you will need to express both a Cas protein and a target-specific gRNA in...

  11. CRISPR Plasmids - Prime Edit

    Type
    Collection
    ...Publication Empty Prime Editing gRNA Vectors A selection of empty gRNA vectors suitable for prime editing are...below. Use the search bar to find a gRNA vector based on expression system, promoter, the type of gRNA (e.g...Cas9. ID Plasmid Expression System Promoter Guide RNA Type Cloning Enzyme Co-expressed Cas9 Selection PI...Libraries gRNAs Empty gRNA Vectors gRNAs By Species Mammalian Bacteria Drosophila Plant C. elegans Yeast Zebrafish... editing plasmids. Plasmids are available for expression in mammalian systems, bacteria, plants, and Drosophila...which a reverse transcriptase (RT) is fused to the C terminus of Cas9 H840A nickase. The fusion enzyme ...-Puro Mammalian, piggyBac hU6 pegRNA BsmBI No Puromycin Jacob Giehm Mikkelsen 173222 pPBT-PE2-PuroTK-pegRNA_GG...

  12. SARS-CoV-2 Pseudotyped Virus

    Type
    Collection
    ...reporter vector expressing firefly luciferase. pCMV-FLuc - Retroviral reporter vector expressing firefly...Lentiviral vector expressing destabilized EGFP. Lenti-luciferase-P2A-Neo - Lentiviral reporter vector expressing...IRES-ZsGreen-W - Lentiviral dual reporter vector expressing firefly luciferase and ZsGreen. NL4-3 mCherry...mCherry Luciferase - Lentiviral dual reporter vector expressing mCherry and firefly luciferase. pLentiEGFPdestablized...Viral Vectors 101: Pseudotyping Antibody Neutralization Response Against Pseudoviruses Expressing SARS-...can then be used to infect permissive cells that express ACE2, the cellular receptor for SARS-CoV-2, in ...Resources page for more information. Plasmids Expressing Spike and Spike Variants Full-length wildtype...

  13. CRISPR Plasmids - Drosophila

    Type
    Collection
    ...Promoter PI Publication Empty gRNA Expression Vectors Select a gRNA expression plasmid based on factors such...choose does not also express a gRNA, you will need to use a separate gRNA expression plasmid to target the...Libraries gRNAs Empty gRNA Vectors gRNAs By Species Mammalian Bacteria Drosophila Plant C. elegans Yeast Zebrafish...cloning method. When using CRISPR, you will need to express both a Cas protein and a target-specific gRNA in...both the gRNA and Cas protein act as all-in-one vectors, but their function is often limited to a single...On the other hand, gRNA plasmids that do not co-express a Cas protein can be paired with a wide variety...Promoter Cloning Enzyme(s) Delivery Resistance Co-expressed Cas9 Depositing lab Cas9 species = S. pyogenes...

  14. Botman-Teusink Yeast FP Collection

    Type
    Collection
    ...Vickers, C. E., Bydder, S. F., Zhou, Y., Nielsen, L. K. (2013). Dual gene expression cassette vectors with...Botman et al., 2019 . FP Tagging Yeast tagging vectors to create fusions of proteins of interest with ...yeast codon-optimized fluorescent proteins. These vectors are based on the pFA6a-link plasmid (Sheff et al...FPs in yeast. Vectors are based on the pDRF1 plasmid (Loqué et al., 2007) containing the PMA1 promoter and...blue, green, and red fluorescent protein tagging vectors for S. cerevisiae. PLoS One, 8 (7):e67902. https...Marker Tags Publication FP Overexpression Plasmids for constitutive overexpression of codon-optimized FPs ...Find plasmids to overexpress various fluorescent markers and tag your proteins of interest with fluorescent...

  15. Malate Dehydrogenase CUREs Community Collection

    Type
    Collection
    ...codon-optimized for expression in bacteria and cloned into an IPTG-inducible His-tag expression vector. All His-tags...sustainable MDH-related CURE focusing on protein expression, structure and function, kinetics and mechanisms...His-tags are cloned at the C-terminus of MDH and, if indicated on the plasmid map, a TEV cleavage site ...Organism or species (e.g., human, watermelon, Streptomyces ) Subcellular compartment (chloroplastic, cytoplasmic...

  16. CRISPR References and Information

    Type
    Collection
    ...cloning vector Retroviral vectors: neomycin (pSIR-neo) , GFP (pSIR-GFP) , DsRed (pSIR-DsRed-Express2) , human... sgRNA dual expression: pAC2 , pAC152 , pAC153 , pAC154 ; pmax dCas9 activator expression: pAC91 , pAC92... pooled library amplification 1 vector system: lentiCRISPR v2 2 vector system: lentiCas9-Blast and lentiGuide-Puro...Addgene Church gRNA design and cloning gRNA cloning vector PDF, 118 KB Church gRNA design and cloning for ...human pluripotent stem cells pCas9_GFP ; gRNA empty vector Protocol at StemBook O'Connor-Giles Fly: gRNA and...PDF, 330 KB Parrott NEBuilder Assembly of CRISPR vectors using ssDNA oligos p201G Cas9 ; p201B Cas9 ; p201H...design and cloning All-in-one plasmid containing expression cassette for NmCas9 and BsmBI site for cloning...
Showing: 1 - 16 of 16 results