We narrowed to 13 results for: cas9 expression construct

...selection of plasmids expressing different functional variants of Cas9. A CRISPR/Cas9 toolkit for multiplex...1000000171 zCas9i Cloning Kit MoClo-compatible vectors for assembly of CRISPR/Cas9 constructs using a high...and species-specific gene expression. Cloning and Synthetic Biology Expression Vectors The table below ...Includes modules for CRISPR/Cas9 genome editing, dexamethasone-inducible expression, and fluorescent plasma...full selection of plasmids expressing different functional variants of Cas9. ID Plasmid Description PI... Nicotiana benthamiana . Plasmids for expressing sgRNA and Cas9 in rice . Includes a protocol (Link opens...

...resources include plasmids expressing Cas9, empty gRNA backbones, and constructs for endogenous tagging. ... genes by feeding worms bacteria expressing dsRNA or use CRISPR/Cas9 gene editing to easily engineer specific...characterized which allow researchers to express desired constructs in precise cell types and/or developmental... via a CRISPR-Cas9 system. Cloning-free CRISPR for C. elegans , which uses purified Cas9, such as that...that expressed from pHO4d-Cas9 from Michael Nonet. Protocol can be found at the (Link opens in a new window...and spatial control of gene expression in C. elegans by combining expression of the FLP recombinase from...Worm Plasmids and Resources Plasmid Tools Worm Constructs Kits and Collections Other Resources The nematode...

...conversion to a dual guide construct. Plasmids 175573 and 175574 express dCas9-KRAB-MeCP2 for repression... and 159655 are single guide expressing double stranded break constructs. Plasmids 171098 and 175570 allow...U6-guide cassettes to make dual guide expressing plasmids dCas9-KRAB-MeCP2 fusion for gene and non-coding...enhancer sequence repression dCas9-VPR fusion for gene activation CRISPR/Cas9 Plasmid Variants Figure 1:...repression. Plasmids 175571 and 175572 express dCas9-VPR for activation. Usage Guide sequences can be designed...in retinal development the lab utilizes a CRISPR/Cas9 based approach to knockout, repress, and activate...taken advantage of the Zhang lab all-in-one (guide/Cas9/reporter) pX458 plasmid family. They have made several...

...Plasmids: pX330S-2-PITCh - expresses Cas9 & PITCh-gRNA pX330A-FBL/PITCh - expresses Cas9, PITCh-gRNA, & FBL-specific...Doyon lab, eSpCas9(1.1)_No_FLAG_AAVS1_T2 (Addgene #79888) , which expresses an untagged Cas9 AND a gRNA... that uses PCR to generate both an expression cassette for the Cas9-programming sgRNA and HR donors for...: 3xFLAG-2xSTREP Construct for integrating at the AAVS1 "safe harbor" locus: eSpCas9(1.1)_No_FLAG_AAVS1...has been adapted to allow tagging of proteins expressed from their natural chromosomal context. These ... consists of two components: A vector containing Cas9 and a validated gRNA, based on the Zhang lab's PX458...need to design and clone in a gRNA(s) to guide the Cas9 protein to your target sequence, as well as design...

...sites for S. pyogenes Cas9, S. aureus Cas9, S. thermophilus Cas9, N. meningitidis Cas9, or Cas12a from your...vectors using ssDNA oligos p201G Cas9 ; p201B Cas9 ; p201H Cas9 ; p201N Cas9 ; pUC gRNA Shuttle PDF, 500 KB...CRISPR RNA array: Cas9 (pX260) or Cas9 D10A (pX334) ; tracrRNA: Cas9 (pX330) or Cas9 D10A (pX335) PDF,...and selection for Cas9-triggered homologous recombination Cas9-sgRNA target construct ; pMA122 (negative...FLAG tagged dCas9 PDF, 110 KB Goldstein Nematode: gRNA design and cloning Cas9-sgRNA construct PDF, 364 ...118 KB Church gRNA design and cloning for Cas9 orthologs Cas9 plasmids PDF, 107 KB Chen and Wente Zebrafish...vitro transcription, injection gRNA core ; Cas9 ; optimized Cas9 PDF, 66.8 KB Fujii gRNA design and cloning...

... 52962 lentiCas9-Blast Sp Cas9 Cut Blasticidin Expresses human codon-optimized S. pyogenes Cas9 protein...backbone. Zhang Cas9 and Accessories for Activating Gene Expression Catalytically-dead Cas9 (dCas9) can be fused...Root Cas9 Lentivirus Cas9 viruses can be used to interrogate the genome in a variety of ways. Cas9 proteins...plasmids resource page . Cas9 for Editing Genomic Sequences To perform genome edits, Cas9 nuclease is used to...Resistance Activity PI 61422 dCAS9-VP64_GFP dCAS9 (D10A, H840A) none Expresses dCAS9-VP64 activator with 2A ...Addgene's viral vector packaging service. Browse constructs currently available in lentiviral format. Viral...Browse In-Stock Lentivirus Pooled CRISPR Libraries Cas9 Pooled Barcoding Libraries Control Addgene's lentiviruses...

...resources for a full selection of plasmids expressing Cas9 and empty gRNA backbones. A few examples of... Using CRISPR/Cas9 - Jeffrey Essner Lab CRISPR-Cas/RNA-Guided Nuclease (RGN) expression plasmids - Keith...Lab CRISPR/Cas9-based conditional mutagenesis in zebrafish - Wenbiao Chen Lab A CRISPR/Cas9 Vector System...fusion expression in organisms or cells in which an exogenous mRNA can be injected and expressed. EXPRESs...in zebrafish. Zebrafish expression atlas - A zebrafish-specific gene expression database that provides ...modified CRISPR-Cas9 system - Shuo Lin Lab Re-purposed Ac/Ds transgenic system enabling CRISPR/dCas9-interference...morpholinos, zinc finger nucleases, TALENS, and CRISPR/Cas9-based gene editing make the zebrafish a stalwart...

...shRNA Expression: Cre-lox can be used to turn shRNA constructs on or off. In floxed-shRNA constructs, Cre...return gene expression to physiological levels. In lox-STOP-lox shRNA constructs, Cre expression promotes...genome or control gene expression or skip ahead to browse highlighted plasmids expressing Cre , Flp , Dre ,...Recognition Sites. Controlling Recombinase Expression By expressing the enzyme at specific times or locations...popular recombinase-dependent gene expression strategies. Gene Expression: A stop codon flanked with loxP...promotes shRNA expression. Gene Switch: These constructs contain two genes of interest, Genes A and B. When ...toxicity. Leakiness Leaky expression, where the gene of interest is expressed even in the absence of the...

... while also adding additional filters such as expression, vector type, species, and more. Plasmids Browse...for Golden Gate assembly. MoClo Toolkit Plant Expression Sylvestre Marillonnet 95 plasmids for cloning...standardized parts to create eukaryotic multigene constructs. ONE-GO Biosensors Kit Fluorescent Proteins Mikel...Stiewe Use these donor vector libraries for CRISPR/Cas9-mediated saturation genome editing of human TP53...

... available with multiple Cas9 variants, including high fidelity Cas9s, Cas9s with various PAM requirements...to either co-infect with a Cas9-producing lentivirus or use a Cas9-expressing stable cell line. After applying...strand SpCas9-HF1 — disrupt Cas9’s interactions with DNA phosphate backbone HypaCas9 — increase Cas9 proofreading...more Cas9 fusion tools later in the guide. Figure 3: Overview of Cas9n. Increasing fidelity of Cas9 Researchers...enhanced specificity by engineering Cas9 into high fidelity Cas9s (hfCas9) . There is currently no defined...application. Cas9 Orthologs While SpCas9 is the most commonly used Cas enzyme, additional Cas9 orthologs ...Additionally, constructs with Cas9 fused to proteins that modulate HDR or NHEJ components, like miCas9 , Cas9...

...astrocyte-selective expression in AAV Dual promoter Separate expression pRGEB31 - Deliver sgRNA and Cas9 into plants... to express a gene; however, we have included a function section for if you want to stably express an ...genetic element, or want to express shRNA. Choose by: Species-Specific Expression Epitope Tag or Fusion Protein...Selectable Markers Species-Specific Expression If you want to drive expression of your favorite gene, you will...EF1a, CAG, Ubc Transient expression - Gradia Lab plasmids for mammalian expression, Ligation Independent ...Empty mammalian expression plasmid for cloning untagged proteins pcDNA3.1-mCherry - Expresses mCherry tag ... Inducible Expression page Bacteria Lac, T7, araBAD, trp mTagBFP2-pBAD - Protein expression vector with...

...tet-inducible Cas9-2A-EGFP expression. Based on LentiCRISPR v2 . Adam Karpf 167935 pLenti-tetON-KRAB-dCas9-DHFR-EF1a-TagRFP...Tetracycline (Tet) Inducible Expression Tetracycline (Tet) Inducible Expression Tetracycline Off Tetracycline...factors to further minimize background expression. Improved expression of the transactivator proteins in mammalian...Experimental Considerations Expression System If your gene of interest should only be expressed occasionally, use...Backbones Find backbones or constructs to use for cloning to control expression of your gene of interest....Top Transactivators (tTA or rtTA) Find a construct that expresses the transactivator for your tetracycline...PB-EF1a-TetOn3G PiggyBac vector expressing Tet-On 3G transactivator constitutively expressed by EF1a promoter Tet-On...

... Plasmid Collections Bacterial Expression Systems Bacterial Expression Systems Protein Purification Protein...bacterial expression systems. Read below and browse our curated collection of bacterial expression plasmids...Addgene Blog E. Coli Strains for Protein Expression Protein Expression Epitope Tags Protein Tags Return to ...to top Controlled Expression It isn’t always advantageous to constitutively express your gene of interest...turn on expression only at a specific time. This collection contains plasmids whose expression levels ...Addgene Controlled Expression Resources Check out our Tetracycline (Tet) Inducible Expression Collection for...systems. ID Plasmid Promoter Inducer Expression Species PI 44249 pdCas9-bacteria pTetO Anhydrotetracycline...