...version of Firefly luciferase that has been codon optimized for expression in mammalian systems. Other ... Click-beetle red luciferase has also been codon optimized ( CBR2opt ) for mammalian expression systems...luciferase. Transcriptional Element Screening Optimized STARR-seq ( S elf- T ranscribing A ctive R egulatory...

...functionality. The Open Bioeconomy Lab designed and codon-optimized the Open Enzymes parts for expression in Escherichia...

...Blast Sp Cas9 Cut Blasticidin Expresses human codon-optimized S. pyogenes Cas9 protein and blasticidin resistance...

...p53 for single plasmid reprogramming Novel codon-optimized mini-intronic plasmid for efficient, inexpensive... Fibroblasts Cardiomyocytes Lentiviral Mouse Optimization of direct fibroblast reprogramming to cardiomyocytes...transcriptionally enriched signaling pathways to optimize the conversion of fibroblasts into neurons. Elife...

... frameshift mutations leading to premature stop codons within the open reading frame (ORF) of the targeted...see Plan Your Experiment below). In addition to optimizing gRNA design, CRISPR specificity can also be increased...Cas9’s. Further advancements have been made by optimizing expression of the fusions, modifying the linker.... gRNA design for CRISPR libraries is usually optimized to select for gRNAs with high on-target activity...proceeding with your experiment, it may be necessary to optimize your delivery conditions. Learn more...shifting the ORF and/or creating premature stop codons NHEJ N on- H omologous E nd J oining; A DNA repair...sequence ORF O pen R eading F rame; The translated codons that make up a gene PAM P rotospacer A djacent ...

...Gene Type Wildtype Mutant Partial Sequence Codon-Optimized gRNA Other Tags GFP FP other Flag GST His TEV... Pickrell 131792 pHAGE-TBK1-C-FLAG-HA TBK1 STOP codon CMV ALS Alicia Pickrell 131889 pDONR221_SLC1A3 SLC1A3...