We narrowed to 10 results for: dual sgRNA

...described here. #60224 - AAV:ITR-U6-sgRNA(Kras)-U6-sgRNA(p53)-U6-sgRNA(Lkb1)-pEFS-Rluc-2A-Cre-shortPA-KrasG12D_HDRdonor-ITR...lentiCRISPR - sgRNA and SpCas9 together 2 vector system - lentiCas9-Blast and lentiGuide-Puro (sgRNA) Browse...has both single- and dual-vector systems that allow the delivery of Cas9 and sgRNA in vivo via adeno-associated...its single guide RNA (sgRNA) into one plasmid (Ran et al., Nature 2015). The dual vector system uses S....CMV-driven SaCas9; U6-driven sgRNA 61593 : PX602; TBG-driven SaCas9; U6-driven sgRNA 61592 : PX600; CMV-driven... SaCas9 sgRNA can be co-transfected as PCR amplicons using a protocol similar to SpCas9 sgRNA PCR (Ran... pAAV-U6::sgRNA(SapI)_hSyn-GFP-KASH-bGH (SpGuide acceptor). This is a AAV plasmid for sgRNA cloning. GFP-KASH...

...Agrobacterium-mediated transformation Dual-sgRNA_hU6-mU6 - Lentiviral expression plasmid for two sgRNAs from an hU6 and an...astrocyte-selective expression in AAV Dual promoter Separate expression pRGEB31 - Deliver sgRNA and Cas9 into plants by...Zeocin®/Bleo Mammalian lenti sgRNA(MS2)_zeo backbone - 3rd gen lenti sgRNA cloning backbone with MS2 loops...selectable marker pMSCV-U6sgRNA(BbsI)-PGKpuro2ABFP - For retroviral delivery of one sgRNA pMKO.1 GFP - Retroviral...metallothionein promoter pFastBac LIC and pFastBac Dual LIC - Vectors from the Gradia lab pFB-LIC-Bse - ...His-tagged versions of pFastBac LIC vectors and pFastBac Dual LIC vectors for insect expression Fluorescent proteins...confers hygromycin resistance pRGEB32 - Express sgRNA/PTG with rice snoRNA U3 promoter and Cas9 with rice...

...for transcriptional activation dCas9 activators sgRNA dual expression: pAC2 , pAC152 , pAC153 , pAC154 ;... Sabatini and Lander gRNA cloning into pLX-sgRNA pLX-sgRNA PDF, 129 KB Sontheimer gRNA design and cloning... can identify positively or negatively selected sgRNAs, genes, or pathways. For use with genome-scale ... opens in a new window) for lentiviral screens. sgRNA Scorer 2.0 (Link opens in a new window) A tool that... and a ctivation (Link opens in a new window) A sgRNA design tool for genome editing, as well as gene ...Link opens in a new window) Identifies candidate sgRNA target sites by off-target quality. Validated for... (Link opens in a new window) Identification of sgRNA target sites and off-target evaluation for base ...

...104321 tet-pLKO-sgRNA-puro Lentiviral Tet-On plasmid for inducible expression of sgRNA TetR H1-2O2 Nathanael...rtTA, respectively. You should also choose between: Dual vector system: express the transactivator and the...may not have as much flexibility and control as a dual vector design, but it is easier to implement and...

...pGL3basic luciferase and renilla_polyA Firefly, Renilla Dual reporter vector. Insertion of 5' promoter/enhancer...Lynne Postovit 64034 pGL4Luc-RLuc Firefly, Renilla Dual reporter reporter vector allowing the study of biscistronic...Daniel Christophe 119760 pSGDlucV3.0 Firefly, Renilla Dual reporter vector. Insertion of 5' promoter/enhancer...Luciferase Type Promoter Description PI 60226 AAV:ITR-U6-sgRNA(backbone)-pEFS-Rluc-2A-Cre-WPRE-hGHpA-ITR Renilla...Voytas 181934 pC0037 Gaussia, Cypridina EF1α, CMV Dual secreted luciferase reporter (EF1α-Gaussia luciferase...

...systems contain two components: a guide RNA ( gRNA or sgRNA ) and a CRISPR-associated endonuclease (Cas enzyme...applications include editing multiple genes at once; using dual nickases to generate a knockout or gene edit; or...using a prime editing guide RNA ( pegRNA ). Like sgRNA, pegRNA directs the nickase to the target site by...efficiency PE3 — increased efficiency; an additional sgRNA directs Cas9n to a nearby site, driving mismatch...- S trand B reak, a break in both strands of DNA Dual nickase/Double nick A method to decrease off-target...in nature and is also commonly referred to as an sgRNA. gRNA scaffold sequence The sequence within the ... J. A., & Charpentier, E. (2012). A programmable Dual-RNA–Guided DNA endonuclease in adaptive bacterial...

..., Doudna JA, Charpentier E. 2012. A programmable dual-RNA-guided DNA endonuclease in adaptive bacterial...labeling of genomic loci with dCas9 and engineered sgRNAs using CRISPRainbow. Nat Biotechnol . . PMID: 27088723...separate the individual crRNAs. (3) The tracrRNA and Cas9 nuclease form a complex with each individual, unique...pre-crRNA). (2) The pre-crRNA is processed into individual crRNAs by a special trans-activating crRNA (tracrRNA...

...Nicotiana benthamiana . Plasmids for expressing sgRNA and Cas9 in rice . Includes a protocol (Link opens...and a variety of other species. In addition to individual plasmids, Addgene carries several useful toolkits...

...an expression cassette for the Cas9-programming sgRNA and HR donors for selectable genome modification...guide RNA transcript and a repair template for an individual tagging event. The kit contains 26 plasmids; ...

...Walker Jackson 78624 sgRNA(MS2)_Prnp_SAM1 PRNP U6 Dementia Walker Jackson 78625 sgRNA(MS2)_Prnp_SAM2 PRNP...Hyman 87200 pX335 HTT sgRNA-b HTT U6 Huntington's Mahmoud Pouladi 87201 pX335 HTT sgRNA-a HTT U6 Huntington's...His T5 Parkinson's Suzanne Pfeffer 208392 Lenti-sgRNA-Cre-GpNLuc-sgmATM ATM Ataxia telangiectasia Gaorav...GFP, FKBP CMV ALS Sascha Martens 208875 pFastBac-Dual- GST-TEV-TBK1 TBK1 polH ALS Sascha Martens 209078...TARDBP-Exon1-ko-sgRNA1-SpCas9 TARDBP U6 ALS Rajat Rohatgi 107858 pHBS953 TARDBP-Exon2-ko-sgRNA2-SpCas9 TARDBP...133762 px459-TDP-43 sgRNA1 TARDBP U6 ALS Shawn Ferguson 133763 px459-TDP 43 sgRNA2 TARDBP U6 ALS Shawn...TIA1 His, T7 T7 ALS Thomas Tuschl 106108 px458_2A_GFP_sgRNA_EIF2AK2 EIF2AK2 U6 VWM disease Thomas Tuschl ...