Skip to main content
Addgene
Open Menu

Enter a sequence to perform a BLAST-based search of Addgene plasmid sequence databases.

Information

In Category

We narrowed to 17 results for: gfp fluorescence reporter

Showing: 1 - 17 of 17 results

  1. Bacterial Expression Systems

    Type
    Collection
    ...ratio Fluorescence (GFP) Escherichia coli Gary Yellen 187836 pVoPo-01 Promoter activity Fluorescence (mCherry...activity Fluorescence (mCherry) Escherichia coli Mattheos Koffas 173481 HC-M ATP Fluorescence (GFPmut2) Escherichia... Addgene Blog A Guide to Selecting Fluorescent Dyes and Ligands GFP Fusion Proteins — Making the Right...Right Connection Photoactivatable Fluorescent Proteins Fluorescent Protein Timers Return to top Protein Interactions...Optogenetics for E. coli Return to top Reporter Plasmids Reporter plasmids can be used to detect events...containing easily measurable reporter genes (e.g., LacZ, luciferase, or fluorescent proteins) under the control...proteins and specific DNA sequences. ID Plasmid Reports Reporter Mechanism Expression Species PI 14474 pRU1161...

  2. Fluorescent Protein Guide: Biosensors

    Type
    Collection
    ...cyclic AMP) Signaling reporter island (SiRI) with GFP-based fluorescent reporter cAMPr Spatial Multiplexing... Plasmid Collections Fluorescent Proteins Fluorescent Biosensors Fluorescent Proteins: Biosensors Metal...Cell Physiology Browse All Other Reporters Additional Resources Fluorescent biosensors are proteins designed...signal of a circularly permuted fluorescent protein (cpFP) or fluorescence resonance energy transfer (FRET...Calcium erGAP3 (GFP-Aequorin Protein) for imaging of Ca 2+ dynamics in endoplasmic reticulum GFP-Aequorin Protein...sensor (GECI) that reports with lifetime and intensity changes A turquoise fluorescence lifetime-based biosensor...orange-emitting fluorescent proteins. Nat Commun. 2017 Sep 5;8(1):431. Wolf Frommer Calcium Orange fluorescent calcium...

  3. Empty Backbones - Choosing Your Perfect Plasmid Backbone

    Type
    Collection
    ...expression Fluorescent proteins (GFP, mCherry, etc) Localization pcDNA3-EGFP - C-terminal GFP for mammalian...expression vector GFP Varies pMKO.1 GFP - Retroviral shRNA expression pLenti CMV GFP DEST - ... expression (Gateway) pYIC - Bicistronic fluorescent reporter gene with cap-dependent 3Myc-EYFP-HA-His6...genome MSCV-IRES-GFP or pMSCV-IRES-mCherry FP - Mammalian retroviral gene expression with GFP or mCherry selectable...luciferase reporter Promoter Measure promoter strength pBV-Luc - Luciferase reporter plasmid... of EBFP2 blue fluorescent protein 35S-eGFP-nosT - Transient expression vector for eGFP in plants Find...fusing your protein to another protein, such as GFP, which allows you to visualize the cellular localization...

  4. Luciferase Plasmid Collection

    Type
    Collection
    ... 64034 pGL4Luc-RLuc Firefly, Renilla Dual reporter reporter vector allowing the study of biscistronic ...Multiple Multi-luciferase reporter vector including five transcriptional reporters for NF-kb, TGF-b, c-Myc...Resources Promega Plasmid Collection Fluorescent Proteins Guide Fluorescent Biosensor Plasmids Blog: Luminescence...Root 105533 pAAV.CMV.Luc.IRES.EGFP.SV40 Firefly CMV AAV expression of firefly luciferase and GFP James Wilson...for gene expression assays and bioluminescent reporters. Plasmid...Collection Empty Backbones Expression Constructs Reporter Constructs Other Highlights Additional Resources...-lives, making them useful as transcriptional reporters, but are less-than-ideal protein tags due to their...

  5. Brain Initiative Collection

    Type
    Collection
    ...pAAV-CAG-FLEX-Archon1-KGC-EGFP-ER2 AAV production plasmid encoding for Archon1 fluorescent voltage reporter, Cre-dependent...Fishell 83895-AAV1 pAAV-hDlx-Flex-GFP-Fishell_6 Cre recombinase-dependent GFP expression in forebrain GABA-...Fishell 83895-AAV2 pAAV-hDlx-Flex-GFP-Fishell_6 Cre recombinase-dependent GFP expression in forebrain GABA-...Fishell 83895-AAV8 pAAV-hDlx-Flex-GFP-Fishell_6 Cre recombinase-dependent GFP expression in forebrain GABA-...Fishell 83895-AAV9 pAAV-hDlx-Flex-GFP-Fishell_6 Cre recombinase-dependent GFP expression in forebrain GABA-...Fishell 83895-AAVrg pAAV-hDlx-Flex-GFP-Fishell_6 Cre recombinase-dependent GFP expression in forebrain GABA-...element Gordon Fishell 83900-AAV1 pAAV-mDlx-GFP-Fishell-1 GFP expression in forebrain GABA-ergic interneurons...

  6. Lentiviral Prep Service

    Type
    Collection
    ...express fluorescent proteins. ID Name Insert Antibiotic Resistance Purpose PI 17446 pLenti CMV GFP Hygro... 61422 dCAS9-VP64_GFP dCAS9 (D10A, H840A) none Expresses dCAS9-VP64 activator with 2A GFP Zhang 61425 .... This library expresses EGFP for easy visualization via direct fluorescence. Morris 115644 Morris CellTag.... This library expresses EGFP for easy visualization via direct fluorescence. Morris 115645 Morris CellTag.... This library expresses EGFP for easy visualization via direct fluorescence. Morris Control Lentivirus...Hygro (656-4) GFP Hygromycin 3rd gen lentiviral eGFP expression vector, CMV promoter, Hygro Campeau Viral...on each item's material page. Actual titers are reported with each shipment. For more details about our...

  7. Viral Production

    Type
    Collection
    ... later, Cre-dependent GFP expression was detected with direct fluorescence. GFP was not detected in the...expression and/or function. These data are sometimes reported or posted on the material page for the corresponding...viral genomes (vg)/cell, pAAV-CAG-FLEX-rc [Jaws-KGC-GFP-ER2] (Addgene 84445-AAVrg) alone at 1.1E6 vg/mL, ... prep # 55632-AAVrg). pAAV-CAG-FLEX-rc [Jaws-KGC-GFP-ER2] was a gift from Edward Boyden (Addgene viral...recipient's initial thaw will be accounted for in our reported titers. Lentiviral vectors are titered using a...

  8. Serotype Testing AAV

    Type
    Collection
    ...: Serotype Testing AAV These AAV encode fluorescent reporters and can be used to compare the tropism of...AAV1). AAV Vectors for Serotype Testing pAAV-CAG-GFP (Plasmid #37825) Description : Ready-to-use AAV in...plasmid 37825 (deposited by Edward Boyden ) and direct GFP expression from the CAG promoter. For information... available from Addgene's viral service. Control EGFP vectors in various serotypes for serotype testing... 37825-AAVrg.T 20 µL $ 150 Add to Cart pAAV-hSyn-EGFP (Plasmid #50465) Description : Ready-to-use AAV ...plasmid 50465 (deposited by Bryan Roth ) and direct EGFP expression from the human synpasin promoter. For...

  9. Rinehart Lab Phosphoprotein Reagents

    Type
    Collection
    ...68299 GFP E17TAG/Q157TAG Plasmid 68298 GFP S2TAG/E17TAG Plasmid 68297 GFP Q157TAG Plasmid 68296 GFP S2TAG...expression vector encoding a split mCherry fluorescent reporter, enabling the detection of phosphorylation-dependent...phosphosite, mCherry fluorescence is restored, so interactions can be discovered by fluorescence-activated cell...Library 111704 Mode #1 Library Plasmid 69118 E17TAG GFP zeo resistance Plasmid 68307 SupD Strain 68306 C321...S2TAG Plasmid 68295 GFP E17TAG Plasmid 68294 SepOTSν Plasmid 68292 SepOTSλ Plasmid 68291 SepOTSκ Plasmid...

  10. Worm Expression Resources

    Type
    Collection
    ... which makes them attractive for imaging and fluorescence microscopy experiments. The C. elegans genome...genome is well studied and hundreds of reporters have been developed and characterized which allow researchers...This includes CRISPR, RNAi, empty backbones, fluorescent proteins, and Cre expression plasmids. Large ...vectors for C. elegans research, including lacZ and GFP fusion vectors. C. elegans optimized fluorophores...Approach to Better CRISPR/Cas9 Editing Efficiency Fluorescent Tagging of Endogenous Genes with SapTrap Even...

  11. Cre-Lox and Other Site-Specific Recombinases

    Type
    Collection
    ...to top Recombinase-Dependent Reporter Plasmids Incorporating a fluorescent protein into your expression...Plasmids Recombinase-Dependent Empty Backbones Reporter Plasmids Pooled Libraries/Kits Site-specific recombinase...recombinases , as well as useful empty backbones or reporters . Experimental Considerations Recombinase Recognition...DOG New Tricks: Controlling Protein Activity with GFP Plasmids 101: FLEx Vectors Optimizing AAV DIO and...visualize which cells have undergone recombination. Reporter plasmids can also be useful for lineage tracing... Plasmid Collections AAV Enhancer Collection Fluorescent Biosensors Chemogenetics Plasmid Collection Optogenetics...

  12. Fluorescent Proteins: FRET

    Type
    Collection
    ... Plasmid Collections Fluorescent Proteins FRET Fluorescent Proteins: FRET FRET Pairs FRET Calibration...use FRET to report on protein binding or conformational changes. Organic dyes, fluorescent proteins, and...Check out our Biosensors Collection to find fluorescent reporters for metal ions, small molecules, and more...Calibration Standards Additional Resources Förster (or Fluorescence) Resonance Energy Transfer (FRET) is a process...experiments. FRET efficiency can be measured using fluorescence microscopy or spectroscopy by exciting the donor...emission of the acceptor. Alternatively, the fluorescence lifetime (the time between excitation of the...a photon) of the donor can be measured using fluorescence lifetime imaging microscopy (FLIM) or FLIM-FRET...

  13. Zebrafish Plasmid Collection

    Type
    Collection
    ...for genome editing, empty backbones, fluorescent proteins, reporters, biosensors, and Cre expression plasmids...markers, somatic mutations, or labeling with fluorescent proteins to visualize and track clones of cells... Albert Pan and Alex Schier Labs. Multicolor fluorescent labeling in zebrafish Whole organism lineage ...studying diseases, are listed below: Lipid droplet reporters - Richard White Lab. An imaging platform to study...study lipid dynamics in vivo. Bimolecular fluorescence complementation (BiFC) tools - Marco Morsch Lab...Michel Bagnat lab. Targeting cassettes encoding fluorescent proteins for tagging genes at endogenous genomic...using red light. LipoGlo - Steven Farber Lab. A reporter system that allows direct assessment of lipoprotein...

  14. CRISPR Plasmids - Tagging

    Type
    Collection
    ... general cloning plasmid, and a prebuilt Unc-32::GFP targeting vector. Jorgensen Lab SapTrap CRISPR/Cas...with the following article: Natsume, et al. Cell Reports 2016 Förstemann Drosophila Cell Tagging System ...provide PCR templates for amplification of the tag (eg GFP, Flag, YFP, etc) and selection markers. Two independent... the first publicly available collection of fluorescently tagged, human, induced pluripotent stem cell...PITCh system plasmids for CRISPR-based knock-in of EGFP-2A-PuroR cassette to the C-terminus of endogenous...the donor vector. The PITCh donor plasmid with an EGFP-2A-PuroR cassette, flanked by microhomologous sequences...first deposited PITCh plasmids were tested by fusing EGFP-2A-PuroR cassette to a nucleolar protein, fibrillarin...

  15. Biosensor AAV Preps

    Type
    Collection
    ...KGC-GFP-ER2 Syn Archon1 EGFP Constitutive 8 Edward Boyden 115893 pAAV-Syn-FLEX-rc [Archon1-KGC-GFP-ER2...Yulong Li Voltage Reporter: Archon 108422 pAAV-CAG-FLEX-Archon1-KGC-EGFP-ER2 CAG Archon1 EGFP Cre dependent...KGC-GFP-ER2] Syn Archon1 EGFP Cre dependent 8 Edward Boyden Voltage Reporter: Voltron 119036 pAAV-hsyn-flex-Voltron-ST...Service: Biosensor AAV Browse our AAV that express fluorescent biosensor proteins, including calcium sensors...-ST none Cre dependent 1 GENIE Project Voltage Reporter: JEDI-2P 179459 pAAV-EF1a-DIO-JEDI-2P-Kv-WPRE ...none Constitutive 9 Francois St-Pierre Voltage Reporter: ASAP 225707 pAAV-hsyn-ASAP5-Kv2.1-WPRE Syn ASAP5...HaloCaMP1a 138327 pAAV-synapsin-HaloCaMP1a-EGFP Syn HaloCaMP1a EGFP Constitutive 1, 9 Eric Schreiter Calcium...

  16. Tetracycline Inducible Expression

    Type
    Collection
    ... evaluate shRNA efficacy by fluorescence, use Plasmid #35626 as a reporter for cDNA target. rtTA3 Ptet...Iwasato 63704 pRetroX GFP T2A Cre Retroviral vector for dox-inducible expression of GFP T2A Cre recombinase...Eric Kowarz 96930 XLone-GFP Tet-On PiggyBac vector for inducble expression of EGFP Tet-On 3G rtTA TRE3GS...171123 pLVX-TetOne-Puro-GFP Lentiviral Tet-On vector for inducible expression of EGFP Tet-On 3G rtTA TRE3GS...pTet-IRES-EGFP Lentiviral plasmid for Tet-controlled expression of transgene of interest with EGFP (On or... Off) None TRE, miniCMV Maria Lung 16542 pBI-MCS-EGFP Bidirectional promoter (Pbi) for Tet-responsive ...expression (On or Off) of both your gene of interest and EGFP. Pbi contains a TRE between two minimal CMV promoters...

  17. CRISPR Guide

    Type
    Collection
    ...sequences using a fluorescent reporter. In SHERLOCK, a quenched ssRNA fluorescent reporter is added to the...catalytically inactive dCas9 to a fluorescent marker like green fluorescent protein (GFP), creating a customizable...viral genome, and a ssDNA-fluorescently quenched reporter is added. As this reporter is degraded by Cas12a...customizable DNA or RNA label for fluorescence microscopy. RNA aptamers, like MS2 or PP7, can also be fused to...to the gRNA to recruit fluorescently-tagged RNA-binding proteins (RBPs). Multicolor CRISPR imaging can...dCas9 and S. aureus dCas9) tagged with different fluorescent proteins or by fusing the gRNAs to orthogonal...imaging of genomic loci, as in the CRISPR-Sirius . Fluorescent CRISPR systems have been used for dynamic tracking...
Showing: 1 - 17 of 17 results