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Showing: 1 - 11 of 11 results

  1. Plasmids for Stem Cell Research

    Type
    Collection
    ... the expression of human Oct4, Klf4, c-Myc, and Sox2 Integrative Analyses of Human Reprogramming Reveal...412-24. Mikkelsen Lentivirus Human Expression of human Klf4, Oct4, c-Myc, and Sox2 as VP-16 transcriptional...Description Article PI Lentivirus Human Expression of human Oct4, Sox2, Klf4, Myc, and Brd3R from five separate...Retrovirus Human Yamanaka factors for generating human iPS cells; retroviral expression of human Sox2, Oct3...-72. Yamanaka Plasmid Human Non-integrating transient expression of human L-Myc, Lin28, Sox2, Klf4, Oct3...episome Human Non-integrating EBNA1-mediated polycistronic expression of human Sox2, KLF4, L-Myc, Lin28...episome Human Fluorescent-tagged EBNA1-mediated expression of human Oct3/4, shp53, Klf4, Sox2, L-Myc, Lin28...

  2. Fluorescent Protein Guide: Subcellular Localization

    Type
    Collection
    ...pTag-RFP-C-h-Rab5a-c-Myc Early endosomes Rab5a TagRFP James Johnson 79801 pTag-BFP-C-h-Rab5a-c-Myc Early ...pTag-RFP-C-h-Rab4a-c-Myc Recycling endosomes Rab4a TagRFP James Johnson 79799 pTag-BFP-C-h-Rab4a-c-Myc Recycling...-RFP-C-h-Rab11a-c-Myc Recycling endosomes Rab11a TagRFP James Johnson 79805 pTag-BFP-C-h-Rab11a-c-Myc ...pCMV-mGold-Tubulin-C-18 Microtubules alpha-tubulin mGold Francois St-Pierre 158009 pCMV-mGold-Actin-C-18 Actin ... NLS mKalama1 Robert Campbell 73205 pcDNA3.1-NLS-myc-dL5-2xG4S-mCer3 Nucleus NLS (from Mak16p protein)...p63 mTurquoise2 Dorus Gadella 73206 pcDNA3.1-kappa-myc-dL5-2xG4S-TMst Cell surface (mammalian) PDGFR-derived...SKL mNeonGreen Dorus Gadella 73210 pcDNA3.1-KozATG-myc-dL5-2XG4S-mCer3-SKL Peroxisome SKL tripeptide, peroxisome...

  3. Neurodegeneration Plasmid Collection

    Type
    Collection
    ...pCMVTNT PINK1 N-myc PINK1 Myc CMV Parkinson's Mark Cookson 13314 pCMVTNT PINK1 C-myc PINK1 Myc CMV Parkinson's...bleo human Frataxin FXN Friedreich ataxia Ronald Kahn 11443 pBS human Frataxin (myc tag) FXN Myc T7 Friedreich...Ultra-Exon1Q23-Myc-A HTT Myc UbC Huntington's Baoji Xu 110487 Ultra-Exon1Q145-Myc-A HTT Myc UbC Huntington's...pUltra-Exon1Q23-Myc-B HTT Myc UbC Huntington's Baoji Xu 110489 pUltra-Exon1Q145-Myc-B HTT Myc UbC Huntington's...Strep-Lrrk2-Myc LRRK2 Myc, Strep CMV Parkinson's Maik Hintze 161583 pPuro3.1(+)_Strep-Lrrk2-Myc LRRK2 Myc, Strep...LRRK2-WT LRRK2 His, Myc CMV Parkinson's Ted Dawson 17612 pRK5-Myc-Parkin PRKN Myc CMV Parkinson's Ted ... pGW1-Myc-DJ1-WT PARK7 V5 CMV Parkinson's Mark Cookson 29349 pGW1-Myc-DJ1-L166P/K130R PARK7 Myc CMV Parkinson's...

  4. CRISPR Pooled gRNA Libraries

    Type
    Collection
    ...Knockout Human Lu 3rd 10 8,205 Bassik Human CRISPR Knockout Library 101926 — 101934 Knockout Human Bassik...103,269 Human CRISPR Knockout Library 1000000132 Knockout Human X.S. Liu 3rd 10 185,634 Human Epigenetic Knockout...plasmid) Knockout Human Zhang 3rd 6 123,411 Human genome-wide library v1 69763 Knockout Human Wu 3rd 4 77,406...,406 Human improved genome-wide library v1 67989 Knockout Human Yusa 3rd 5 90,709 Human Interferon-Stimulated...Activation Human Zhang 3rd 10 96,458 Human CRISPR Metabolic Gene Knockout Library 110066 Knockout Human Sabatini...,658 Human Genome-Wide Reduced Double-gRNA Library 137999 Knockout Human Parts 3rd 3 59,576 Human Whole...Knockout Human Vakoc 3rd 6 3,051 Human Lipid Droplet and Metabolism Library 191535 Knockout Human Olzmann...

  5. Luciferase Plasmid Collection

    Type
    Collection
    ...including transcriptional reporters for NF-kb, TGF-b, c-Myc, p53 and MAPK/JNK transcriptional reporters Koen...Mammalian expression of Nanoluc with a N-terminal Myc tag Erich Wanker 124701 pLenti-PGK-Venus-Akaluc (...Lentiviral expression of Nanoluc with a N-terminal Myc tag Erich Wanker 115352 pFL-SV40 Firefly SV40 Mammalian... CIneo-RL Renilla CMV Mammalian expression of humanized renilla luciferase Witold Filipowicz 100984 pGL4.18...pathway in vivo . DULIP ( DU al L uminescence-based C o- I mmunoprecipitation) plasmids : Luminescence-based...fusing NanoLuc® and Venus fluorophore to Troponin C Ca 2+ binding domain. Nano-lanterns , Enhanced Nano-lanterns...87075 pLenti6.2-ccdB-Nanoluc NanoLuc® Creation of C-terminal Nanoluc fusions using Gateway cloning. Lentival...

  6. Cre-lox system

    Type
    Collection
    ...Cepko 13779 pRho-Cre Cre-Myc rhodopsin Mammalian Cepko 13780 pNrl-Cre Cre-Myc, Expressed in rod photorecetor...the human Estrogen Binding Domain (EBD) TDH3 Yeast van Leeuwen 64770 pSS146 Cre fused to the human Estrogen...recombinant Cre Bacterial Rajewsky 13775 pCAG-Cre Cre-Myc CAG Mammalian Cepko 13776 pCAG-Cre:GFP Cre-GFP fusion... a circular piece of DNA (and is not maintained). C) If the sites are on separate DNA molecules (in trans... Cre: Cre recombinase is split in half into N and C terminal Cre fragments (NCre and CCre, respectively...Bacterial Ekker 47551 pDD104 (Peft-3::Cre) Cre eft-3 C. elegans Goldstein 48201 CAG-GFP-IRES-CRE Cre and ...system CAG Mammalian Pelczar 51268 pCAG-Co-InCreC C-terminal component of the Co-InCre system CAG Mammalian...

  7. CRISPR References and Information

    Type
    Collection
    ...supports: Drosophila, Arabidopsis, zebrafish, C. elegans, mouse, human, rat, cow, dog, pig, Thale cress, rice... cerevisiae ), worm ( C. elegans ), fruit fly, zebrafish, mouse, rat, and human. CCTop - CRISPR/Cas9 target...include Arabidopsis, C. elegans , sea squirt, cavefish, Chinese hamster, fruit fly, human, rice fish, mouse...supports: Drosophila, Arabidopsis, zebrafish, C. elegans, mouse, human, rat, rabbit, pig, possum, chicken, dog...: Drosophila, Arabidopsis, zebrafish, C. elegans , mouse, human, rat, yeast, frog, Brachypodium distachyon...Retroviral vectors: neomycin (pSIR-neo) , GFP (pSIR-GFP) , DsRed (pSIR-DsRed-Express2) , human CD2 (pSIR-hCD2...location, and genome annotation. Available genomes are human (hg19 & hg38), mouse (mm10), and yeast (strain w303...

  8. CRISPR History and Development for Genome Engineering

    Type
    Collection
    ...Mammalian systems (Human, mouse, and rat) Bacteria ( E. coli , Streptococcus , Streptomyces , and others) ...editing. These edits can include mutations seen in human patients, protein tags, or loxP/FRT sites, among...germline editing, with the International Summit of Human Gene Editing convened in 2015 to discuss the ethics...2013. Dynamic imaging of genomic loci in living human cells by an optimized CRISPR/Cas system. Cell . ... Approach for Mapping Multiprotein Complexes in Human Cells. Cell Rep . 13(3):621-33. PMID: 26456817 Fu...Multicolor CRISPR labeling of chromosomal loci in human cells. Proc Natl Acad Sci U S A . 112(10):3002-7...DiCarlo JE, Norville JE, Church GM. 2013. RNA-guided human genome engineering via Cas9. Science . 339(6121)...

  9. CRISPR Plasmids - Prime Edit

    Type
    Collection
    ...optimized PE2 enzyme containing RT optimized for human codons, additional nuclear localization signals,...gRNAs By Species Mammalian Bacteria Drosophila Plant C. elegans Yeast Zebrafish Xenopus CRISPR Resources ...which a reverse transcriptase (RT) is fused to the C terminus of Cas9 H840A nickase. The fusion enzyme ...-Puro Mammalian, piggyBac hU6 pegRNA BsmBI No Puromycin Jacob Giehm Mikkelsen 173222 pPBT-PE2-PuroTK-pegRNA_GG...

  10. Malate Dehydrogenase CUREs Community Collection

    Type
    Collection
    ...based on: Organism or species (e.g., human, watermelon, Streptomyces ) Subcellular compartment (chloroplastic...expression vector. All His-tags are cloned at the C-terminus of MDH and, if indicated on the plasmid map...

  11. CRISPR Plasmids - Empty gRNA Vectors

    Type
    Collection
    ...Cas9 + Empty sgRNA) 47549 C. elegans yes, cut S. pyogenes Goldstein DR274 42250 C. elegans BsaI none S. pyogenes...Mammalian/AAV SapI none S. pyogenes Zhang pgRNA-humanized 44248 Mammalian/Lentiviral BstXI + XhoI none S...gRNAs By Species Mammalian Bacteria Drosophila Plant C. elegans Yeast Zebrafish Xenopus CRISPR Resources ...pyogenes Zhang PX854 62886 Mammalian BbsI yes, cut, C-terminal S. pyogenes Zhang pGuide 64711 Mammalian ...pyogenes Zhang PX852 62884 Mammalian BbsI yes, cut, C-terminal S. pyogenes Zhang PX855 62887 Mammalian BbsI...pyogenes Zhang PX856 62888 Mammalian BbsI yes, activate, C-terminal S. pyogenes Zhang pU6-(BbsI)_CBh-Cas9-T2A-BFP-P2A-Ad4E1B...pyogenes URA3 Wyrick pCRISPomyces-2 61737 Bacteria BbsI yes, cut S. pyogenes Apramycin Zhao pRB1017 59936...
Showing: 1 - 11 of 11 results