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  1. Joung Lab CRISPR-Cas/RNA-Guided Nuclease (RGN) Plasmids

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    ...the Cas9 endonuclease) that cleaves the target DNA. The Joung lab recently described gRNA and Cas9 expression...specifity of Staphylococcus aureus Cas9 (SaCas9 KKH variant) Engineered CRISPR-Cas9 nucleases with altered PAM...) CRISPR-Cas/RGN expression plasmids for use in human cells CRISPR-Cas/RGN expression plasmids for use...Gene Guide RNA expression plasmids for endogenous human genes Guide RNA expression plasmids for endogenous...Type II CRISPR-Cas systems, are programmable endonucleases that can be used to perform targeted genome ... (AsCpf1 and LbCpf1) for use in mammalian cells SpCas9 variants with no detectable off-target effects ...

  2. CRISPR History and Development for Genome Engineering

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    ...High fidelity Cas9s: Three high fidelity Cas9 variants , SpCas9-HF, eSpCas9, and HypaCas9 display very ...III and Cas9 enzymes, which together separate the individual crRNAs. (3) The tracrRNA and Cas9 nuclease...Norville JE, Church GM. 2013. RNA-guided human genome engineering via Cas9. Science . 339(6121):823-6. PMID: ...Zhang F. 2014. Genome-Scale CRISPR-Cas9 Knockout Screening in Human Cells. Science . 343(6166):84-7. PMID...Lander ES. 2014. Genetic Screens in Human Cells Using the CRISPR/Cas9 System. Science . 343(6166):80-4. ...Staphylococcus aureus (SaCas9) is smaller than SpCas9 and more easily packaged in AAV. Cas9 orthologs also have...beyond SpCas9, moving us closer to being able to target every locus in every genome. Multiple Cas9 orthologs...

  3. CRISPR Guide

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    ... available with multiple Cas9 variants, including high fidelity Cas9s, Cas9s with various PAM requirements...about Cas9 multiplexing . Browse Plasmids: Multiplex gRNA Vectors Engineering the Cas9 Endonuclease CRISPR...recognition; inhibiting Cas9’s endonuclease domain; or inducing degradation of the Cas9 protein. Most Acr proteins...strand SpCas9-HF1 - disrupt Cas9’s interactions with DNA phosphate backbone HypaCas9 - increase Cas9 proofreading...new tools. Figure 3: Overview of Cas9n Nickase and dead Cas9 The two Cas9 nuclease domains, RuvC and HNH...enhanced specificity by engineering Cas9 into high fidelity Cas9s (hfCas9) . There is currently no defined...application. Cas9 Orthologs While SpCas9 is the most commonly used Cas enzyme, additional Cas9 orthologs ...

  4. CRISPR References and Information

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    ...vectors using ssDNA oligos p201G Cas9 ; p201B Cas9 ; p201H Cas9 ; p201N Cas9 ; pUC gRNA Shuttle PDF 488.6 ...CRISPR RNA array: Cas9 (pX260) or Cas9 D10A (pX334) ; tracrRNA: Cas9 (pX330) or Cas9 D10A (pX335) ; PDF...putative target sites for S. pyogenes Cas9, S. thermophilus Cas9, or Cpf from your input sequence or list...fruit fly, zebrafish, mouse, rat, and human. CCTop - CRISPR/Cas9 target online predictor Identifies candidate...thermophilus or N. meningitidis Cas9 PAMs. Currently supports: mouse and human. CRISPR Optimal Target Finder....2 KB Church gRNA design and cloning for Cas9 orthologs Cas9 plasmids PDF 104.1 KB Chen and Wente Zebrafish...vitro transcription, injection gRNA core ; Cas9 ; optimized Cas9 PDF 66.8 KB Fujii gRNA design and cloning...
Showing: 1 - 4 of 4 results