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We narrowed to 17 results for: lentiviral dna vectors

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Showing: 1 - 17 of 17 results

  1. Viral Production

    Type
    Collection
    ... the lentiviral vector, harvesting cells several days later, and isolating genomic DNA. This DNA is then...for in our reported titers. Lentiviral vectors are titered using a lentiviral droplet digital PCR (ddPCR...with serial dilutions of a lentiviral vector, incubated for 72 h, and genomic DNA is extracted. Primers and... . Browse our AAV vectors collection. AAV Data Hub Lentivirus Production Lentiviral preparations are produced... with viral vector manufacturing facilities, such as the University of Pennsylvania Vector Core. Transfections...Pennsylvania Vector Core. In general, titering is performed by the facility that produced the viral vector lot....Empty vector particles can be identified after negative staining and appear darker than full vector particles...

  2. Empty Backbones - Choosing Your Perfect Plasmid Backbone

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    Collection
    ...compatible Y2H vector - BD pRPR1_gRNA_handle_RPR1t - gRNA expression empty vector pFA6a vectors - Epitope ...Tet-inducible HA-tagged lentiviral vector for ORF expression pDest-565 - Gateway destination vector for bacterial... - Tet-inducible lentiviral Gateway destination vector for gene expression Lentiviral backbones made by... Tet-inducible lentiviral shRNA expression pLKO.1 - TRC cloning vector - Lentiviral shRNA expression...Blasticidin Mammalian pLX304 - 3rd gen lentiviral vector lentiSAMv2 - Lentiviral sgRNA cloning backbone pLKO.1-...Flag tag; 2NLS; pCDNA3 vector backbone, mammalian expression pENTR4-myc-nuc - pEntry vector that adds a C-terminal...pFastBac Dual LIC - Vectors from the Gradia lab pFB-LIC-Bse - Insect expression vector from Gileadi lab ...

  3. Viral Vector Guides and Plasmids

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    Collection
    ...Guides and Plasmids Lentiviral Vector Guide | Lentiviral Plasmids Lentiviral vectors are versatile tools...Guides Viral Vectors Viral Vector Guides and Plasmids Selecting a Viral Vector Type Introduction to Viral...Viral Vectors Resources Viral vectors are popular tools for delivering genetic information to cells. These...the host genome. AAV Vector Guide | AAV Plasmids Adeno-associated viral (AAV) vectors are commonly used ... Gamma-retroviral Vector Guide | Gamma-retroviral Plasmids Gamma-retroviral vectors, often referred to...Retroviral vectors are popular for making stable cell lines or clinical use. Adenoviral Vector Guide | Adenoviral...applications as vaccines. Selecting a Viral Vector Type Each viral vector type exhibits different properties and...

  4. Plan Your Experiment

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    Collection
    ...viral vectors and their production, see our viral vector guides . Lentiviral vectors Lentiviral vectors... in-stock lentiviral preps . AAV vectors AAV vectors offer some advantages for viral vector delivery. ...delivery, lentiviral and adeno-associated virus (AAV) vectors are the most common. Using viral vectors takes... CRISPR plasmids . Delivery Using Viral Vectors Viral vectors are a great option, as they offer high expression...CRISPR components) integrates into the genome. Lentiviral vectors are a good option for difficult-to-transfect...don’t discuss CRISPR screens in this guide, lentiviral vectors are a common choice for conducting genome-wide.... Both lentiviral and AAV vectors are safe to use in the lab, but AAVs exhibits the lowest immunogenicity...

  5. Luciferase Plasmid Collection

    Type
    Collection
    ...cells Peter Cockerill 212936 pGL3 Basic Vector Firefly Vector for investigating regions controlling transcription... Scott Lowe 21375 pHIV-Luciferase Firefly Lentiviral vector for insertion of your gene of interest upstream... regions. Lentiviral Nadav Ahituv 212935 pGL4.84(hRlucCP/Puro) RapidResponse™ Renilla Vector for investigating...Janes 99283 pCW57.1-Luciferase Firefly TRE Lentiviral vector with dox- or tet-inducible luciferase expression...Firefly CMV Lentiviral expression of firefly luciferase Eric Campeau 18964 Luciferase-pcDNA3 Firefly CMV... Firefly EF1α Lentiviral expression of firefly luciferase Javier Alcudia 113442 pcDNA3.1 NL NanoLuc® CMV...N-terminal Nanoluc fusions using Gateway cloning. Lentiviral. Mikko Taipale 12563 PCD FL0X Firefly Insertion...

  6. Tetracycline Inducible Expression

    Type
    Collection
    ...plasmid. A single vector approach may not have as much flexibility and control as a dual vector design, but ...popular empty backbones, expression vectors for transactivators, and vectors for other applications, or search...Ptet, miniCMV Howard Gu 44012 pInducer20 Lentiviral Tet-On vector for inducible expression. See article ...Xiaojun Lian 171123 pLVX-TetOne-Puro-GFP Lentiviral Tet-On vector for inducible expression of EGFP Tet-On...TRE3GS Jason Sheltzer 11651 pLVUT-tTR-KRAB Lentiviral Tet-On vector for inducible expression of transgene ...Aebischer , Didier Trono 41393 pCW57.1 Lentiviral Tet-On vector for inducible expression; insert with ...Tight TRE David Root 100521 pCW57.1-MAT2A Lentiviral Tet-Off vector for repressible expression with blasticidin...

  7. CRISPR Plasmids and Resources

    Type
    Collection
    ...information into a specific DNA site. Base Edit Base editors precisely edit DNA without promoting DSB formation...visualize genomic loci. gRNAs Empty gRNA Vectors Select an empty vector for your experiment based on a variety...Request ready-to-use viral preps of select CRISPR lentiviral plasmids. CRISPR Plasmid Kits : Find groups of...Mutated Cas nickase enzymes generate single-strand DNA breaks. Prime Edit Cas nickase enzymes fused to reverse...Insert CRISPR transposases insert large fragments of DNA. Transcriptional Regulation Activate dCas9 fused ...Targeting Type VI CRISPR systems target RNA, not DNA, across multiple systems. RNA Editing Cas13 enzymes...

  8. CRISPR Guide

    Type
    Collection
    ...purify specific regions of DNA, image DNA in live cells, and precisely edit DNA and RNA. The ease of generating...pooled format into lentiviral transfer vectors (Figure 8B). Libraries come in 1-vector systems, in which...-stranded DNA (dsDNA) plasmid. Small single-stranded DNA (ssDNA) oligos often work well for small edits... two formats: as liquid DNA, or in select cases, as pre-made lentivirus . DNA libraries are shipped at...homology with the target DNA. Once the Cas9-gRNA complex binds a putative DNA target, the seed sequence...will begin to anneal to the target DNA. If the seed and target DNA sequences match, the gRNA will continue...strands of the target DNA. This results in a double-strand break (DSB) within the target DNA, located ∼3–4 nucleotides...

  9. Lentivirus Plasmids

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    Collection
    ... safety. Lentiviral Vector Guide Popular Lentiviral Transfer Plasmids Looking for lentiviral plasmids ...at Addgene. Read our Lentiviral Vector Guide for more information about lentiviral components, generations... Additional Resources Lentiviral Vector Guide Addgene’s Viral Service Lentiviral Pooled Libraries All ...lab. One safety feature of lentiviral vectors is that the components necessary to produce an infectious ...Viral Vectors 101 eBook Biosafety Resource Guide Addgene blog: Tips for Titering Your Lentiviral Preps...transgene expression. Wild-type lentiviruses have been engineered into viral vectors for safe use in the lab....Addgene lentiviral packaging, envelope, and transfer plasmids Viral...

  10. Zhang Lab CRISPR Page

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    Collection
    ...- #61427 are intended for lentivirus production : 61425 : lentiviral vector encoding dCAS9-VP64 with 2A... in two formats: 1 vector system - lentiCRISPR - sgRNA and SpCas9 together 2 vector system - lentiCas9... 2A Blast resistance marker 61426 : lentiviral vector encoding the MS2-P65-HSF1 activator helper complex...Nature 2015). The dual vector system uses S. pyogenes Cas9 (SpCas9), using one vector to express SpCas9, ...61408 : Targeting vector for the mouse Rosa26 locus; Used to make Cas9 knockin mouse Vectors for cancer modeling...SpCas9 or SpCas9n, and the single guide RNA. The vector can be digested using BbsI, and a pair of annealed...indicated below) can be cloned scarlessly into the vector before the sgRNA scaffold. The oligos are designed...

  11. Neurodegeneration Research Collection

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    Collection
    ...transcription factors in a single polycistronic lentiviral vector . (Addis et al. PLoS One. 2011) See More Antibodies...Nat Methods. 2024) Base edit human mitochondrial DNA using mitoBEs . (Yi et al. Nat Biotechnol. 2023) ...more. Convert iPSCs to neurons with inducible lentiviral expression constructs: pLVX-UbC-rtTA-Ngn2:2A:...with rapid delivery. Includes antibodies, viral vectors, animal models, purified protein, and more. Plasmids...(Link opens in a new window) such as huntingtin cDNAs, antibodies, and cell lines. Neurodegeneration Method...

  12. Fluorescent Protein Guide: Empty Backbones

    Type
    Collection
    ...Expression (this is a bicistronic vector rather than a fusion protein vector) ZsGreen1-N1 - Mammalian Expression...Mammalian Gateway Destination Vector pENTR4-HaloTag (w876-1) - Gateway Entry Vector pCS2+/C-Halo , pCS2+/N-Halo...Gateway Entry Vector pCS2+/C-SNAPf , pCS2+/N-SNAPf - Xenopus Expression pSNAP-tag (T7) Vector - Bacterial...pCLIP-tag (m) Vector - Mammalian Expression pENTR4-CLIPf (w877-2) - Gateway Entry Vector pET51b-His-TEV-CLIP-tag...mCherry 587 610 16 4.5 15 min Monomer pcDNA3 mCherry LIC cloning vector (6B) - Mammalian Expression pLV-mCherry...or search our complete collection and filter by vector type, popularity, and more. Blue/UV Protein Excitation...Prone to dimerization pLV-Azurite - Mammalian Lentiviral expression pB18cmBFPAzurite - Bacterial Expression...

  13. CRISPR References and Information

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    Collection
    ... gRNA design and cloning gRNA cloning vector Retroviral vectors: neomycin (pSIR-neo) , GFP (pSIR-GFP) ... pooled library amplification 1 vector system: lentiCRISPR v2 2 vector system: lentiCas9-Blast and lentiGuide-Puro... KB Parrott NEBuilder Assembly of CRISPR vectors using ssDNA oligos p201G Cas9 ; p201B Cas9 ; p201H Cas9...CRISPOR batch (Link opens in a new window) for lentiviral screens. sgRNA Scorer 2.0 (Link opens in a new...Addgene Church gRNA design and cloning gRNA cloning vector PDF, 118 KB Church gRNA design and cloning for ...PDF, 108 KB Fujii iChIP/enChIP to purify genomic DNA FLAG tagged dCas9 PDF, 110 KB Goldstein Nematode:...human pluripotent stem cells pCas9_GFP ; gRNA empty vector Protocol at StemBook O'Connor-Giles Fly: gRNA and...

  14. Viral Prep Service for Plasmids and Pooled Libraries

    Type
    Collection
    ...technologies. Penn Vector Core AAV Vectors Many AAV vectors from the Penn Vector Core have been transferred to Addgene...of particular Penn Vector Core catalog items. Virus-associated DNA Each viral vector service request will...the viral vector prep you need to get your experiments started more quickly. Each viral vector is produced...publications. All pricing includes DNA amplification, viral vector production, purification, and comprehensive...biosafety of viral vectors, and general biosafety guidelines. Addgene Blog: Viral Vector Posts Content from...aspects of viral vectors, including troubleshooting advice and explanations of viral vector technologies....also include virus-associated DNA, which is a sample of the purified DNA from the original plasmid that...

  15. Plasmid Collections

    Type
    Collection
    ...Proteins Protein Expression Synthetic Biology Viral Vectors Communities Find a scientific community that has...Luciferase Optogenetics Chemogenetics Viral Plasmids Lentivirus Adeno-associated Virus (AAV) γ-Retrovirus Adenovirus...Types Barcoding CRISPR Screening Surface Display cDNA Depositor Collections A modular set of plasmids ...

  16. Neurodegeneration Plasmid Collection

    Type
    Collection
    ...Meyer 114193 pc DNA FRT TO- APP695 APP CMV Alzheimer's Aleksandra Radenovic 114194 pc DNA FRT TO- APPSwed...237005 VCP-HaloTag Vector VCP Halo CMV ALS Promega Corporation 237006 HaloTag-VCP Vector VCP Halo CMV ALS...Other, non-FP Plasmid Type Mammalian, non-viral Lentiviral AAV Retroviral Bacterial Yeast Other Promoter...19 James Trimmer 129409 Slc1a3-CreERT2 Targeting Vector SLC1A3 PGK Episodic ataxia Walker Jackson 129410...ALS Promega Corporation 237148 NanoBRET Assay Vector, TREM2-HaloTag TREM2 Halo CMV Alzheimer's Promega ...Corporation 237172 EIF2AK2(252-551)-NanoLuc Fusion Vector EIF2AK2 NanoLuc CMV VWM disease Promega Corporation...Cookson 13315 pcDNA-DEST53 PINK1 N-GFP PINK1 GFP CMV Parkinson's Mark Cookson 13316 pcDNA-DEST47 PINK1 ...

  17. Immunology Research Plasmids and Resources

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    Collection
    ...Gateway® Entry vectors from the labs of William Hahn and David Root . Multiple Lentiviral Expression (MuLE...histocompatibility complex, class II, DO alpha HLA-DNA, HLA-DZA, HLADZ HLA-DOB major histocompatibility ...systems. The MXS-chaining kit includes chaining vectors with multiple cloning sites and standardized building... reading frames (ORFs) cloned into a retroviral vector which adds a myristoylation sequence and flag-epitope...system that can be used to construct complex lentiviruses for modification of mammalian cells with a single...
Showing: 1 - 17 of 17 results