...expressed in the retina). Fluorescent Cre: The fusion of Cre to a fluorescent reporter enables visualization...pCMV-cre-K Cre-K CMV Mammalian Green 8395 p210 pCMV-CREM CREM CMV Mammalian Green 8401 p224 pCMV-RFP/CREM floxed...within CREM CMV Mammalian Green 8403 p153 pPr-cre Cre probasin Mammalian Green 8404 p236 pPr-CREM CREM ...probasin Mammalian Green 8405 p240 pPr-CREM/Ins CREM with insulators probasin Mammalian Green 8406 p252 pPr-CREM...12238 pLOX-CW-CRE Cre CMV Lentiviral Trono 12265 pHR-CMV-nlsCRE Cre CMV Lentiviral Trono 12493 p259 pCMV-...Mammalian Green 12494 p260 pCMV-CRE-M-AR(wt) CREM fused to AR ligand binding domain CMV Mammalian Green 12496...coexpression CMV Lentiviral Sadelain 30205 Cre-IRES-PuroR Cre and PuroR coexpression EF-1 alpha Lentiviral Kotton...

...Special Collections Fluorescent Protein Guide Empty Backbones Fluorescent Protein Guide: Empty Backbones...extensively by life scientists. The original green fluorescent protein (GFP) was cloned in 1992 ( Prasher et ... mRFP Tsoulfas Lab Lentiviral Plasmids - Fluorescently tag your gene for lentiviral expression Verkhusha...Expression mAzamiGreen 492 505 41 5.8 Monomer mAzami Green-N1 (mAG) - Mammalian Expression mAzami Green-C1 (... backbones with different fluorescent tags for you to create fusion proteins with your gene of interest...Backbones More Fluorescent Protein Resources: FRET Biosensors Subcellular localization Michael Davidson Collection... Imaging Blog: Avoiding the Dark Side of Fluorescent Protein Fusions with mOX FPs Blog: When is a Monomer...

...co-immunoprecipitation to assess protein-protein interactions. NanoLuc® complementation : Protein-protein assays based on... Plasmid Collection You may also like... Fluorescent Proteins Blog: Luciferase 101 Blog: Technologies ...-learning approaches. Split-Luciferase and Protein-Protein Interaction Assays Cluc-FKBP , FRB-Nluc : Split...mmunoprecipitation) plasmids : Luminescence-based protein-protein screening method for the systematic and quantitative...NanoLuc® have been used to assess GPCR-G protein interactions , protein dimerization , endosomal escape of ..., Enhanced Nano-lanterns , and Antares : Fluorescent proteins are fused to Renilla luciferase (Nano-lanterns... promoter/enhancer regions. Lentiviral Nadav Ahituv 108228 pGreen2x35SLUC Firefly/Renilla Creating ORF-Firefly...

...Plasmid Collections Fluorescent Proteins Fluorescent Biosensors Fluorescent Proteins: Biosensors Metal ...Glucose Green fluorescent glucose indicators for living cells (Glifons) Green Fluorescent Protein-Based ...Lactate Lindoblum green fluorescent biosensor for lactate Green fluorescent protein-based lactate and ...Pyruvate Pegassos green fluorescent biosensor for pyruvate Green fluorescent protein-based lactate and...cyclic AMP) Red fluorescent protein-based cAMP indicator (Pink Flamindo) Red fluorescent protein-based cAMP... AAV Biosensors Fluorescent biosensors are proteins designed so that their fluorescent properties are ... from a nested cassette of green- and orange-emitting fluorescent proteins. Nat Commun. 2017 Sep 5;8(1...

...catalytically inactive dCas9 to a fluorescent marker like green fluorescent protein (GFP), creating a customizable...tagged with different fluorescent proteins or by fusing the gRNAs to orthogonal protein-interacting RNA aptamers...naturally-occurring Acr (Anti-CRISPR) proteins can help control CRISPR activity. Acr proteins have evolved in phages ...; or inducing degradation of the Cas9 protein. Most Acr proteins are specific to a particular type of ...CRISPR libraries What Are Pooled Lentiviral CRISPR Libraries? Pooled lentiviral CRISPR libraries are a heterogeneous...and antibody-activator effector proteins; long-term imaging of proteins in living cells (Figure 9B) dCas9...fused to the gRNA to recruit fluorescently-tagged RNA-binding proteins (RBPs). Multicolor CRISPR imaging can...

...plasmid type, mutations, and/or tags such as fluorescent protein (FP) tags. Information on genes and associated...Other, non-FP Plasmid Type Mammalian, non-viral Lentiviral AAV Retroviral Bacterial Yeast Other Promoter...Omi-mCherry HTRA2 mCherry LTR Parkinson's Douglas Green 50487 pSLIK-NFLAG-hFUS FUS Flag, His TRE ALS Zissimos...Multisystem proteinopathy, ALS Nicolas Fawzi 139121 hnRNPA2_LC_NtoS HNRNPA2B1 T7 Multisystem proteinopathy, ALS...Multisystem proteinopathy, ALS Nicolas Fawzi 139123 hnRNPA2_LC_12E HNRNPA2B1 T7 Multisystem proteinopathy, ALS...Multisystem proteinopathy, ALS Nicolas Fawzi 139125 hnRNPF_PLD_StoA HNRNPA2B1 T7 Multisystem proteinopathy, ALS...Erik Roberson 179896 pMKRQ_11_DExCon-mNeonGreen-R25 RAB25 mNeonGreen TRE3GS Parkinson's Patrick Caswell ...