...Backbones CRISPR Genome modification lentiCRISPR v2 - 3rd gen lentiviral vector for mammalian genome editing ...compatible Y2H vector - BD pRPR1_gRNA_handle_RPR1t - gRNA expression empty vector pFA6a vectors - Epitope ...expression vector with N- or C-terminal HA tag pInducer20 - Tet-inducible HA-tagged lentiviral vector for ORF... - Tet-inducible lentiviral Gateway destination vector for gene expression Lentiviral backbones made by... Tet-inducible lentiviral shRNA expression pLKO.1 - TRC cloning vector - Lentiviral shRNA expression...Blasticidin Mammalian pLX304 - 3rd gen lentiviral vector lentiSAMv2 - Lentiviral sgRNA cloning backbone pLKO.1-...pLKO.1 hygro - Lentiviral shRNA expression lentiCRISPRv2 hygro - Variant of lentiCRISPR v2 that confers ...

...Library in lentiCRISPR v2-Blast 1000000173 Knockout Mouse Liu 3rd 10 185,836 Mouse GeCKO v2 1000000052...198,810 CRISPRa-v2 83978 1000000091 Activation Human Weissman 3rd 5 10 104,540 209,080 CRISPRa-v2 83996 1000000093...206,421 CRISPRi-v2 83969 1000000090 Inhibition Human Weissman 3rd 5 10 104,535 209,070 CRISPRi-v2 83987 1000000092...hCRISPRa-v2 subpooled libraries 83980 — 83986 Activation Human Weissman 3rd 5 Varies hCRISPRi-v2 subpooled...mCRISPRa-v2 subpooled libraries 83998 — 84004 Activation Mouse Weissman 3rd 5 Varies mCRISPRi-v2 subpooled...-FokI Screen Pooled Libraries gRNAs Empty gRNA Vectors gRNAs By Species Mammalian Bacteria Drosophila ...Clear Filters Name ID Library Type Species PI Lentiviral Generation gRNAs per gene Total gRNAs Adamson...

... Empty Vectors Many gRNA empty vectors have been deposited at Addgene. To find the gRNA vector for your...genome engineering from a single lentiviral vector Mammalian Lentiviral expression of Cas9, dCas9, or dCas9...50662 Mammalian/Lentiviral See paper none S. pyogenes Blast Sabatini and Lander lentiCRISPR v2 52961 Mammalian...Mammalian/Lentiviral BbsI none S. pyogenes Puro, TagBFP Yusa lentiGuide-Puro 52963 Mammalian/Lentiviral BsmBI...Mammalian/Lentiviral BsmBI none S. pyogenes Zhang pL-CRISPR.EFS.GFP 57818 Mammalian/Lentiviral BsmBI yes...Mammalian/Lentiviral BfuAI none S. pyogenes Puro Wolfe pL-CRISPR.EFS.tRFP 57819 Mammalian/Lentiviral BsmBI...Mammalian/Lentiviral BsmBI none S. pyogenes ccdB Wei pLKO5.sgRNA.EFS.PAC 57825 Mammalian/Lentiviral BsmBI ...

...Familial British dementia Jan Johansson 138347 lentiCRISPR v2-sgDnmt1_1 DNMT1 U6 Hereditary sensory neuropathy...neuropathy type IE Yi Zhang 138348 lentiCRISPR v2-sgDnmt1_2 DNMT1 U6 Hereditary sensory neuropathy type IE...LRRK2 His T7 Parkinson's Suzanne Pfeffer 186025 lentiCRISPR v2-sgCOQ2 COQ2 U6 Multiple system atrophy Boyi...Other, non-FP Plasmid Type Mammalian, non-viral Lentiviral AAV Retroviral Bacterial Yeast Other Promoter...19 James Trimmer 129409 Slc1a3-CreERT2 Targeting Vector SLC1A3 PGK Episodic ataxia Walker Jackson 129410...CMV Alzheimer's Johanna Ivaska 154900 pDEST-SorLA-v2 SORL1 Venus CMV Alzheimer's Johanna Ivaska 156022...OPTN GST T7 ALS Sascha Martens 218527 plentiCRISPR-v2 Puro EIF2AK2/PKR_sgRNA EIF2AK2 U6 VWM disease Kivanc...