Skip to main content
Addgene

Enter a sequence to perform a BLAST-based search of Addgene plasmid sequence databases.

In Category

We narrowed to 10 results for: lentiviral vectors expressing mcherry

Showing: 1 - 10 of 10 results

  1. CRISPR Plasmids - Empty gRNA Vectors

    Type
    Collection
    ...engineering from a single lentiviral vector Mammalian Lentiviral expression of Cas9, dCas9, or dCas9-VP64... Empty Vectors Many gRNA empty vectors have been deposited at Addgene. To find the gRNA vector for your...constructing all-in-one expression vectors containing multiple guide RNA expression cassettes and a Cas9 ...size and allow for expression of more sgRNAs in a single vector. sgRNAs are expressed as polycistronic ... pyogenes mCherry Luikart pDECKO_mCherry 78534 Mammalian hU6 none S. pyogenes Puro, mCherry Guigo BPK3079...gRNA expression vectors for insertion of custom gRNA target sequences. CRISPR...CRISPR Plasmids: Empty gRNA Expression Vectors Browse CRISPR Plasmids By Function Genome Editing Cut Base...

  2. Empty Backbones - Choosing Your Perfect Plasmid Backbone

    Type
    Collection
    ...GFP for mammalian expression pLV-mCherry - 3rd gen lentiviral plasmid expressing mCherry pBad-EBFP2 - Bacterial...Tet-inducible lentiviral shRNA expression pLKO.1 - TRC cloning vector - Lentiviral shRNA expression pLJM1-EGFP...mammalian expression plasmid for cloning untagged proteins pcDNA3.1-mCherry - Expresses mCherry tag in mammalian...compatible Y2H vector - BD pRPR1_gRNA_handle_RPR1t - gRNA expression empty vector pFA6a vectors - Epitope ...LIC vectors and pFastBac Dual LIC vectors for insect expression Fluorescent proteins (GFP, mCherry, etc...plants Find FP tagging vectors in budding yeast pHT101-mCherry - Expresses mCherry tagged fusion proteins... - Tet-inducible lentiviral Gateway destination vector for gene expression Lentiviral backbones made by...

  3. SARS-CoV-2 Pseudotyped Virus

    Type
    Collection
    ...reporter vector expressing firefly luciferase and ZsGreen. NL4-3 mCherry Luciferase - Lentiviral dual reporter...2A-MCS-Hygro - Lentiviral vector expressing destabilized EGFP. Lenti-luciferase-P2A-Neo - Lentiviral reporter... are listed below: pBOBI-FLuc - Lentiviral reporter vector expressing firefly luciferase. pCMV-FLuc - ...Retroviral reporter vector expressing firefly luciferase. pHAGE-CMV-Luc2-IRES-ZsGreen-W - Lentiviral dual reporter...reporter vector expressing mCherry and firefly luciferase. pLentiEGFPdestablized - EFS-EGFPd2PEST-2A-MCS-Hygro...reporter vector expressing firefly luciferase with neomycin resistance. Other Resources Protocols Bloom lab ...Viral Vectors 101: Pseudotyping Antibody Neutralization Response Against Pseudoviruses Expressing SARS-...

  4. Kazuhiro Oka Lentiviral Vectors

    Type
    Collection
    ...Collections Gene Vector Core Viral Vectors Gene Vector Core Viral Vectors Lentiviral Transfer Vectors Adenoviral...information on lentiviral and adenoviral vectors read our Lentiviral Vector Guide and Adenoviral Vector Guide ...series of lentiviral and adenoviral transfer vectors with Addgene. Lentiviral Transfer Vectors Within this... transfer vectors and generating new viral vectors. Download the Lentivirus Transfer Vector Production...Lentiviral Vectors deposited by Kazuhiro Oka from the Baylor College of Medicine Gene Vector Core Depositor...Production Protocol Lentiviral Vector ID Purpose pCDH-EF1-DIO-copGFP 72253 Expresses copGFP under EF-1 promoter...promoter when Cre is expressed by another vector pCDH-EF1-DIO-tdTomato 72254 Expresses tdTomato under EF-1 ...

  5. Viral Production

    Type
    Collection
    ...in the absence of Cre. mCherry expression alone was detected. pAAV-Ef1a-mCherry-IRES-Cre was a gift from...for in our reported titers. Lentiviral vectors are titered using a lentiviral droplet digital PCR (ddPCR... . Browse our AAV vectors collection. AAV Data Hub Lentivirus Production Lentiviral preparations are produced... with viral vector manufacturing facilities, such as the University of Pennsylvania Vector Core. Transfections...Pennsylvania Vector Core. In general, titering is performed by the facility that produced the viral vector lot....Transducibility Some viral vectors are tested in vitro and in vivo for gene expression and/or function. These...Empty vector particles can be identified after negative staining and appear darker than full vector particles...

  6. Lentivirus Plasmids

    Type
    Collection
    ...resistance. Wiederschain 36084 pLV-mCherry 3rd CMV driven expression of mCherry fusion. See plasmids 36083 and...Blog: Tips for Titering Your Lentiviral Preps Lentiviral Pooled Libraries All Viral Plasmids Packaging Plasmids...researcher safety. Read our Lentiviral Guide for more information about: Lentiviral components 2nd versus 3rd...Glossary of commonly used lentiviral terms Popular Lentiviral Envelope and Packaging Plasmids One safety feature...Envelope VSV-G-expressing envelope plasmid Trono 8454 pCMV-VSV-G N/A Envelope VSV-G-expressing envelope plasmid...pCPRDEnv 2nd Packaging part of the FELIX vector system, expresses Gag-Pol and Rev Nolan 35617 pCAG-Eco N...MLV expressing envelope plasmid Nienhuis and Salmon 35616 pCAG-VSVG N/A Envelope VSV-G-expressing envelope...

  7. Fluorescent Protein Guide: Empty Backbones

    Type
    Collection
    ...Mammalian Expression pLV-mCherry - Mammalian Lentiviral Expression pCS2+8CmCherry - Zebrafish/Xenopus/...Mammalian Expression pCAG-GFP - Mammalian Expression pLV-eGFP - Mammalian Lentiviral Expression Ac5-STABLE1...Retroviral Expression mCherry 587 610 16 4.5 15 min Monomer pcDNA3 mCherry LIC cloning vector (6B) - Mammalian...pCS2+8NmCherry - Zebrafish/Xenopus/C.elegans/Sea urchin pET mCherry LIC cloning vector (u-mCherry) - Bacterial...pLV-Azurite - Mammalian Lentiviral expression pB18cmBFPAzurite - Bacterial Expression mAzurite 383 450 14 ...pBad-EBFP2 - Bacterial Expression EBFP2-N1 - Mammalian Expression EBFP2-C1 - Mammalian Expression EBFP2-pBAD - ...- Mammalian Expression pCyPet-C1 - Mammalian Expression pCyPet-His - Bacterial Expression CYPet-N1 - Mammalian...

  8. Tetracycline Inducible Expression

    Type
    Collection
    ...popular empty backbones, expression vectors for transactivators, and vectors for other applications, or...Howard Gu 44012 pInducer20 Lentiviral Tet-On vector for inducible expression. See article ( Meerbrey et...171123 pLVX-TetOne-Puro-GFP Lentiviral Tet-On vector for inducible expression of EGFP Tet-On 3G rtTA TRE3GS...Sheltzer 11651 pLVUT-tTR-KRAB Lentiviral Tet-On vector for inducible expression of transgene and/or shRNA....Didier Trono 41393 pCW57.1 Lentiviral Tet-On vector for inducible expression; insert with Gateway cloning...Root 100521 pCW57.1-MAT2A Lentiviral Tet-Off vector for repressible expression with blasticidin selection...PB-EF1a-TetOn3G PiggyBac vector expressing Tet-On 3G transactivator constitutively expressed by EF1a promoter ...

  9. Retrovirus Plasmids

    Type
    Collection
    ...construct for miRNA expression Chen 52114 pMSCV-IRES-mCherry FP MSCV A bicistronic IRES-mCherry containing retroviral...γ-Retrovirus Guide Biosafety Guide Lentiviral Plasmids AAV Plasmids Viral Vectors 101 eBook γ-Retrovirus (gamma-retrovirus...Ecotropic MLV expressing envelope plasmid Nienhuis and Salmon 35616 pCAG-VSVG Envelope VSV-G-expressing envelope...GFP MoMLV U6-driven plasmid for shRNA expression; also expresses GFP. See pMKO.1 puro for Puro resistance... to eGFP expression Clevers 18760 MSCV IRES Luciferase MSCV Plasmid for transgene expression; also expresses... 9044 pMIG MSCV Plasmid for transgene expression; also expresses GFP. Also see pMIG-w , a variant that...27995 TtRMPVIR CMV/MSV Tet-regulated expression of shRNA; expresses rtTA. See Zuber et al. 2011 for additional...

  10. Fluorescent Protein Guide: Biosensors

    Type
    Collection
    ...Douglas Kim , GENIE Project Calcium Bicistronic vector expressing GCaMP6 and mRuby2 for imaging neural activity...NADH/NAD+ Cytosolic expression of fluorescent NADH/NAD+ biosensor Peredox-mCherry in plants In Vivo NADH...Mitophagy Dual-emission mitophagy biosensor (lentiviral expression of pH sensitive mt-mKeima) Spatiotemporal... Huebener , Tobias Rose Calcium Soma-targeted expression of jGCaMP8f, GCaMP7f, or GCaMP6f Precision Calcium...6. Edward Boyden Calcium Intersectional viral expression of GCaMP6m/s in cells depdent on Cre, Flp, or... Dual- and Triple-Feature Intersectional Single-Vector Delivery of Diverse Functional Payloads to Cells...Karl Deisseroth , INTRSECT 2.0 Project Calcium Expression of GCaMP6f in PV cortical interneurons under ...
Showing: 1 - 10 of 10 results