We narrowed to 11 results for: lentivirus expression plasmid

... Viral Plasmids Lentiviral Plasmids Lentiviral Plasmids Transfer Plasmids Packaging ... VSV-G-expressing envelope plasmid. Bob Weinberg 12259 pMD2.G VSV-G-expressing envelope plasmid. Didier...Packaging Plasmids Envelope Plasmids Resources Lentiviruses, a subset of retroviruses, are some of the most common...Popular Lentiviral Transfer Plasmids Looking for lentiviral plasmids to express a CRISPR gRNA? Browse our...catalog for lentiviral CRISPR plasmids for gRNA expression . ID Plasmid Generation Description PI 8453...destination plasmid for cDNA expression. Miguel Ramalho-Santos 24129 pULTRA 3rd Bicistronic expression of EGFP... 3rd EF-1alpha driven expression of cDNA and and EGFP co-expression. See plasmid 21374 for dTomato version...

...empty gRNA plasmids . For more information on CRISPR plasmids and function, see our CRISPR plasmids resource... Viral Vector Packaging Service Lentivirus Browse In-Stock Lentivirus Pooled CRISPR Libraries Cas9 Pooled...Barcoding Libraries Control Addgene's lentiviruses are made from plasmids that are currently in our repository...library expresses EGFP for easy visualization via direct fluorescence. Morris Control Lentivirus Browse... of available viruses based on plasmids in our repository that express fluorescent proteins. ID Name Insert...Ready-to-use lentivirus available from Addgene's viral vector packaging service. Browse constructs currently...repository. You can obtain the plasmid from us or receive a ready-to-use viral preparation from the inventory...

... Plasmid Collections Plasmids for Stem Cell Research Plasmids for Stem Cell Research...Reprogramming Plasmids Differentiation and Transdifferentiation Plasmids Stem Cell Research Plasmids Stem cells...utilize plasmids to increase expression of key factors. The tables below will help you find plasmids available...information page. Reprogramming Plasmids Browse the table below for plasmids used to create induced pluripotent...Method Species Description Article PI Lentivirus Human Expression of human Oct4, Sox2, Klf4, Myc, and Brd3R...Commun. 2018 Jul 6;9(1):2643. Otonkoski Lentivirus Human Expression of human Sox2, Nanog, Oct4, and Lin28...318(5858):1917-20. Thomson Lentivirus Human Doxycycline-inducible expression of human Oct4, Sox2, Klf4,...

...the transfer plasmid, a plasmid encoding Rep and serotype-specific Cap genes, and a plasmid encoding adenoviral... coli . In addition, plasmids are typically prepared using endotoxin-free plasmid purification kits. Resources...viral production methods and quality control for lentivirus and AAV. Viral...AAV Production Titer Quality Control Resources Lentivirus Production Titer Quality Control Resources Overview...contamination is minimized by using an endotoxin-free plasmid purification protocol. To minimize the immunogenic...vectors are tested in vitro and in vivo for gene expression and/or function. These data are sometimes reported...mL, or both. Two days later, Cre-dependent GFP expression was detected with direct fluorescence. GFP was...

...Homepage Plasmid Collections Addgene Plasmid Collections Addgene's repository contains 167,150 plasmids . We've...Viral Plasmids Lentivirus Adeno-associated Virus (AAV) γ-Retrovirus Adenovirus All Viral Plasmids Curated...Microbiology Plant Expression Stem Cells Synthetic Biology Tet Inducible Expression Worm Expression Kits Kits ...you are looking for a non-plasmid item, browse our entire repository . Plasmid Resources Our curated resources...resources group plasmids together based on type or application. Browse our guides to find the plasmids and science...Curated Topics Antibody Plasmid Collection Bacterial Expression Systems Empty Backbones Genetic Code Expansion...Consortium Krüppel-like factors (KLF) Plasmid Resource RESOLUTE Plasmid Collection for Solute Carrier Proteins...

...collection of SARS-CoV-2 spike plasmids, packaging plasmids, and reporter plasmids for viral pseudotyping with...Addgene's Viral Plasmids and Resources page for more information. Plasmids Expressing Spike and Spike ...Accessory Plasmids Envelope and Packaging Plasmids Popular Lentiviral Envelope and Packaging Plasmids Popular...Envelope and Packaging Plasmids Reporter Plasmids A few examples of reporter plasmids that can be used for...2 Pseudotyped Virus SARS-CoV-2 Spike Plasmids Accessory Plasmids Other Resources Mechanisms of SARS-CoV...circulating variants. The table below lists expression plasmids that are suitable for generating pseudotyped... vector expressing firefly luciferase and ZsGreen. Alejandro Balazs 170674 pBOBI-FLuc Express firefly ...

...Vector Guide Find more plasmids in our Lentiviral Plasmids and Adenoviral Plasmids collections Addgene Blog...reporter plasmids to test whether or not you've efficiently generated infectious virus, plasmids expressing...-EF1-DIO-copGFP 72253 Expresses copGFP under EF-1 promoter when Cre is expressed by another vector pCDH-EF1...pCDH-EF1-DIO-tdTomato 72254 Expresses tdTomato under EF-1 promoter when Cre is expressed by another vector pCDH-CB-iCre-P2A-tdTomato-T2A-Puro...pCDH-CB-copGFP-T2A-iCre 72256 Expresses copGFP and iCre under from the CB promoter pCDH-CB-iCre 72257 Express iCre under ...pCDH-CB-FLPe-P2A-tdTomato 72259 Expresses FLPe and tdTomato from the CB promoter pCDH-EF1-Fon-copGFP 72260 Expresses copGFP from...promoter when FLP is expressed by another vector pCDH-EF1-Fon-tdTomato 72261 Expresses tdTomato from the ...

...multiple gRNAs using a single plasmid ensures that all gRNAs will be expressed in the same cell. This is ...need to generate lentivirus containing the entire library (Figure 8D). This lentivirus will then be used... co-infect with a Cas9-producing lentivirus or use a Cas9-expressing stable cell line. After applying ...tools require constitutive expression of the dCas9 fusion and/or expression of multiple fusions. The effects... DNA. This sequence is cloned into a gRNA expression plasmid but does NOT include the PAM sequence or ...Addgene's guide to using CRISPR plasmids in your lab, from knockouts to pooled library screening. Science...sequences . Once you’ve selected your target, you’ll express your gRNA and Cas9 protein, which will form a ribonucleoprotein...

...tracrRNA: This plasmid contains three expression cassettes. To target a given site, the plasmid can be digested...nuclei in addition to sgRNA expression. This plasmid contains two expression cassettes, Cre recombinase... SpCas9 Plasmids The CRISPR/Cas system can be implemented in mammalian cells by co-expressing the S. pyogenes...., Science 2013). Additionally, we have a plasmid expressing SpCas9 alone without sgRNA. Full references...nickase) + single guide RNA: These plasmids contain two expression cassettes, a human codon-optimized ...sequence. 3. SpCas9 alone: This plasmid contains a single cassette expressing human codon-optimized SpCas9...

...depositors ). Browse empty gRNA expression vectors . Select Your Expression and Delivery System Once you ... your experiment) and the expression time (transient or stable). For plasmid and viral-based approaches...mammalian cells is to directly transfect expression plasmids encoding your Cas protein and gRNA, which...electroporation. Expression can be transient, or you can generate stable cell lines if your plasmid contains ...option, as they offer high expression levels, long-term and stable expression, are safe and easy to work...transfect, transduce, or may not express common promoters. Browse Cas9 plasmids for RNP delivery . As mentioned...and/or Cas9 expression or (2) a lack of efficient target cleavage in cells that do express both Cas9 and...

... Plasmid Collections Immunology Research Immunology Research Plasmids and Resources You...Fluorescent Proteins Cancer Research Plasmids Gene Categories Plasmid Tables Additional Resources Mammalian...to the available plasmids for each gene. Please note that not all genes have plasmids available for request...and apoptosis. Plasmid Tables The gene names in these tables link to the available plasmids for each gene...suggestions for other plasmids that should be added to this list? Fill out our Suggest a Plasmid form or e-mail...Addgene’s immunology collection contains plasmids for genes involved in innate and adaptive immune signaling...finds and attacks pathogens or infected cells expressing that antigen. Antibodies produced by B cells ...