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We narrowed to 18 results for: luciferase expression

Showing: 1 - 18 of 18 results
  1. Luciferase Plasmid Collection

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    Collection
    ... Collections Luciferase Plasmids Luciferase Plasmid Collection Empty Backbones Expression Constructs Reporter... of luciferase found in Addgene's collection include: Firefly luciferase (Fluc) Renilla luciferase (Rluc...respectively), Cypridina luciferase , and Luciola luciferase . Each type of luciferase has advantages and disadvantages...within the luciferase gene allow for increased copy number in E. coli and expression of luciferase in the ...Groskreutz 18760 MSCV IRES Luciferase Firefly Retroviral expression of firefly luciferase and gene of interest...regions upstream of Firefly luciferase. Renilla luciferase is co-expressed. Oskar Laur 87070 pcDNA3.1-... regions upstream of Firefly luciferase. Renilla luciferase is expressed from a T7 promoter for normalization...
  2. SARS-CoV-2 Pseudotyped Virus

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    ... mCherry Luciferase Lentiviral dual reporter vector expressing mCherry and firefly luciferase to simultaneously...105621 Lenti-luciferase-P2A-Neo Lentiviral reporter vector expressing firefly luciferase with neomycin... vector expressing firefly luciferase and ZsGreen. Alejandro Balazs 170674 pBOBI-FLuc Express firefly ...pCMV-FLuc Express firefly luciferase. Used in MLV-based SARS-CoV-2 pseudovirus assay. David Nemazee 161030...Zhou-pLOV-GFP-Luc Lentiviral vector expressing GFP and luciferase for use as a reporter in pseudovirus...can then be used to infect permissive cells that express ACE2, the cellular receptor for SARS-CoV-2, in ...Resources page for more information. Plasmids Expressing Spike and Spike Variants Full-length wildtype...
  3. Promega Plasmid Collection

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    ...NanoLuc luciferase-fusion protein expressed in cells. Additional Resources Browse Addgene's Luciferase Plasmid...Find luciferase reporter vectors for sensitive gene expression analysis and fusion vectors containing...The collection contains luciferase reporter vectors for sensitive gene expression analysis and protein fusion...HaloTag, SmBiT, LgBiT, HiBiT, and more. Luciferase Reporters Luciferase (Link opens in a new window) reporter...bright and stable luciferase that enables highly sensitive detection at endogenous expression levels. NanoLuc..., reporter, protein, or tag, such as "NFAT", "luciferase", "Ras", "NanoLuc", "HaloTag", "BiT", or other... from an enzymatic reaction to measure subtle expression changes with high sensitivity. These assays are...
  4. Retrovirus Plasmids

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    ...18760 MSCV IRES Luciferase MSCV Plasmid for transgene expression with luciferase expression. Scott Lowe 9044...MSV lncRNA expression plasmid. Lei Sun 60683 pLXIN-Luc MoMSV Stable expression of luciferase in mammalian...GFP MoMLV U6-driven plasmid for shRNA expression with GFP expression. See plasmid 8452 for puromycin resistance...9044 pMIG MSCV Plasmid for transgene expression with GFP expression. See plasmid 12282 for the addition...27995 TtRMPVIR CMV/MSV Tet-regulated expression of shRNA. Expresses rtTA. See Zuber et al. (2011) for additional...pMD2.G VSV-G-expressing envelope plasmid. Didier Trono 35617 pCAG-Eco Ecotropic MLV expressing envelope plasmid...Description PI 1780 pBABE-neo largeTcDNA MoMLV Expression of Large T antigen for creation of immortalized...
  5. Empty Backbones - Choosing Your Perfect Plasmid Backbone

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    ... to express a gene; however, we have included a function section for if you want to stably express an ...genetic element, or want to express shRNA. Choose by: Species-Specific Expression Epitope Tag or Fusion Protein...Selectable Markers Species-Specific Expression If you want to drive expression of your favorite gene, you will...EF1a, CAG, Ubc Transient expression - Gradia Lab plasmids for mammalian expression, Ligation Independent ...Empty mammalian expression plasmid for cloning untagged proteins pcDNA3.1-mCherry - Expresses mCherry tag ... Inducible Expression page Bacteria Lac, T7, araBAD, trp mTagBFP2-pBAD - Protein expression vector with...TEV cleavage Transient expression - Gradia Lab plasmids for bacterial expression, Ligation Independent ...
  6. Zhang Lab CRISPR Page

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    ...transfection : 61422 : Expresses dCAS9-VP64 activator with 2A GFP 61423 : Expresses the MS2-P65-HSF1 activator...Cas9 (SpCas9), using one vector to express SpCas9, and another to express up to three sgRNAs (Swiech et al...to LacZ, plus luciferase-2A-Cre recombinase 60226 : sgRNA cloning backbone with luciferase-2A-Cre recombinase...AAV-KPL) This plasmid contains two expression cassettes, Renilla luciferase-2A-Cre recombinase and sgRNAs ...-ITR This plasmid contains two expression cassettes, Renilla luciferase-2A-Cre recombinase and an sgRNA...nuclei in addition to sgRNA expression. This plasmid contains two expression cassettes, Cre recombinase...
  7. Chemogenetics Plasmids

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    ...a scientist to control signaling within cells expressing these engineered receptors. Chemogenetic receptors... the specific cell type where the receptor is expressed, these receptors can be used to artificially activate...luminescent opsins: fusion proteins of a light-emitting luciferase and a light-sensing optogenetic element such ...such as channelrhodopsin. When the luciferase enzyme oxidizes its substrate (luciferin), it generates light...
  8. Bacterial Expression Systems

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    ... Plasmid Collections Bacterial Expression Systems Bacterial Expression Systems Protein Purification Protein...bacterial expression systems. Read below and browse our curated collection of bacterial expression plasmids...Addgene Blog E. Coli Strains for Protein Expression Protein Expression Epitope Tags Protein Tags Return to ...to top Controlled Expression It isn’t always advantageous to constitutively express your gene of interest...turn on expression only at a specific time. This collection contains plasmids whose expression levels ...Addgene Controlled Expression Resources Check out our Tetracycline (Tet) Inducible Expression Collection for...transcription factors. Addgene Blog Luciferase Technologies Enabled by NanoLuc® Luciferase Fluorescent Biosensors ...
  9. Lentivirus Plasmids

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    ...pLentipuro3/TO/V5-GW/EGFP-Firefly Luciferase 3rd Expression of EGFP-Firefly luciferase fusion protein. Ethan Abel...plasmids to express a CRISPR gRNA? Browse our catalog for lentiviral CRISPR plasmids for gRNA expression . ID... (Cre-lox), stable expression of shRNAs; addition of Cre turns on shRNA expression. See Ventura et al....cDNA expression. David Sabatini 14883 FUGW 3rd hUbC-driven EGFP. Can be used for cDNA expression. David... constitutive transgene expression. Contains intron that gives high expression. Didier Trono 12254 pWPI...plasmid for cDNA expression. Miguel Ramalho-Santos 24129 pULTRA 3rd Bicistronic expression of EGFP and the...pHIV-EGFP 3rd EF-1alpha driven expression of cDNA and and EGFP co-expression. See plasmid 21374 for dTomato...
  10. Plasmid Collections

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    ...Microbiology Plant Expression Stem Cells Synthetic Biology Tet Inducible Expression Worm Expression Kits Kits ...Zinc Fingers Luminescence Fluorescent Proteins Luciferase Optogenetics Chemogenetics Viral Plasmids Lentivirus... Topics Antibody Plasmid Collection Bacterial Expression Systems Empty Backbones Genetic Code Expansion...Collection Types CRISPR Fluorescent Proteins Protein Expression Synthetic Biology Viral Vectors Communities Find...
  11. Fluorescent Protein Guide

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    ...signalling pathways. Luciferase Plasmids Use bioluminescent reporters for studying gene expression, signaling pathways...
  12. Fluorescent Proteins: FRET

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    ...fluorophores through linkers, fluorescent protein expression level and maturation time, and other environmental...Read more about self-labeling tags on our blog . Luciferase can be paired with a fluorescent protein acceptor...Resonance Energy Transfer (BRET) . Browse Addgene's Luciferase Plasmid Collection or the Promega Plasmid Collection...
  13. Cre-Lox and Other Site-Specific Recombinases

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    ...genome or control gene expression or skip ahead to browse highlighted plasmids expressing Cre , Flp , Dre ,...Recognition Sites. Controlling Recombinase Expression By expressing the enzyme at specific times or locations...popular recombinase-dependent gene expression strategies. Gene Expression: A stop codon flanked with loxP...return gene expression to physiological levels. In lox-STOP-lox shRNA constructs, Cre expression promotes...toxicity. Leakiness Leaky expression, where the gene of interest is expressed even in the absence of the...catalog for plasmids expressing Cre recombinase and filter the results by expression system and vector type...catalog for plasmids expressing Flp recombinase and filter the results by expression system and vector type...
  14. COVID-19 Resources

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    ...Browse the tables below to find popular plasmids expressing genes encoding SARS-CoV-2 proteins, (spike protein...and codon-optimized versions designed for your expression system. Some of the following plasmids are available...Collection – This page includes a table of all spike expression plasmids in our collection that are suitable ... pseudotyped virus. It also lists several luciferase and fluorescent reporter plasmids that have been...2 plasmids for E. coli , yeast, and mammalian expression, and spike protein constructs...Neutralization Response Against Pseudoviruses Expressing SARS-CoV-2 Spike Protein Variants Return to top...
  15. Tetracycline Inducible Expression

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    ...Tetracycline (Tet) Inducible Expression Tetracycline (Tet) Inducible Expression Tetracycline Off Tetracycline...factors to further minimize background expression. Improved expression of the transactivator proteins in mammalian...Experimental Considerations Expression System If your gene of interest should only be expressed occasionally, use...PB-EF1a-TetOn3G PiggyBac vector expressing Tet-On 3G transactivator constitutively expressed by EF1a promoter Tet-On...Dox-inducible expression of Cre recombinase. Madeline Lancaster 198752 DiLiCre 2.0 Dox-inducible expression of a...Learn about tetracycline-regulated gene expression and find plasmids for your experiments using tet- ... promoters are powerful tools to control gene expression and study biological targets. The tetracycline...
  16. Fluorescent Protein Guide: Empty Backbones

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    ...pBad-EBFP2 - Bacterial Expression EBFP2-N1 - Mammalian Expression EBFP2-C1 - Mammalian Expression EBFP2-pBAD - ...- Mammalian Expression pCyPet-C1 - Mammalian Expression pCyPet-His - Bacterial Expression CYPet-N1 - Mammalian...MiCy-N1 - Mammalian Expression MiCy-C1 - Mammalian Expression MiCy-pBAD - Bacterial Expression Return to top... - Mammalian Expression sfGFP-C1 - Mammalian Expression sfGFP-pBAD - Bacterial Expression moxGFP 486 510...Mammalian Expression pCAG-GFP - Mammalian Expression pLV-eGFP - Mammalian Lentiviral Expression Ac5-STABLE1...1 - Mammalian Expression mEGFP-N1 - Mammalian Expression mEGFP-C1 - Mammalian Expression mEGFP-pBAD - ...Mammalian Expression pET-MCN-10His-mStayGold (E138D) - Bacterial Expression pJRA110 - Plant Expression mStayGold...
  17. Fluorescent Protein Guide: Biosensors

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    ...Douglas Kim , GENIE Project Calcium Soma-targeted expression of jGCaMP8f, GCaMP7f, or GCaMP6f Precision Calcium...6. Edward Boyden Calcium Intersectional viral expression of GCaMP6m/s in cells depdent on Cre, Flp, or...Karl Deisseroth , INTRSECT 2.0 Project Calcium Expression of GCaMP6f in PV cortical interneurons under ... Oct 25;9(1):4440. Eric Schreiter Calcium AAV expression of photoconvertible fluorescent protein-based..., Eric Schreiter Calcium Intersectional viral expression of sRGECO (constitutive or Cre- or Flp-dependent...Olivia Masseck Calcium Astrocyte AAV-mediated expression of jRCaMP1a, a red fluorescent calcium sensor... acetyl-CoA sensor for bacterial or mammalian expression, targeted to nucleus, cytoplasm, or mitochondria...
  18. Control AAV Preps

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    ...Service: Control AAV Browse our control AAV that express fluorescent proteins under general promoters. These... 5, 8 James M. Wilson 105532 pAAV.CMV.ffLuciferase.SV40 CMV ffLuciferase Constitutive 8 James M. Wilson...
Showing: 1 - 18 of 18 results