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We narrowed to 14 results for: luciferase expression

Showing: 1 - 14 of 14 results

  1. Luciferase Plasmid Collection

    Type
    Collection
    ...C-terminal Red Luciferase fusion for expression in plants, co-expressed with Gaussia luciferase for ratiometric...types of luciferase found in Addgene’s collection are Firefly luciferase (Fluc), Renilla luciferase (Rluc...interest before or after luciferase . In addition, Addgene has many luciferase expression constructs available...within the luciferase gene allow for increased copy number in E. coli and expression of luciferase in the ...regions upstream of Firefly luciferase. Renilla luciferase is co-expressed. Oskar Laur 87070 pcDNA3.1-... regions upstream of Firefly luciferase. Renilla luciferase is expressed from a T7 promoter for normalization...sequences downstream of Renilla luciferase. Firefly luciferase expression under the control of a HSV TK ...

  2. SARS-CoV-2 Pseudotyped Virus

    Type
    Collection
    ...reporter vector expressing firefly luciferase. pCMV-FLuc - Retroviral reporter vector expressing firefly luciferase... reporter vector expressing firefly luciferase and ZsGreen. NL4-3 mCherry Luciferase - Lentiviral dual...vector expressing destabilized EGFP. Lenti-luciferase-P2A-Neo - Lentiviral reporter vector expressing firefly...dual reporter vector expressing mCherry and firefly luciferase. pLentiEGFPdestablized - EFS-EGFPd2PEST-2A-MCS-Hygro...can then be used to infect permissive cells that express ACE2, the cellular receptor for SARS-CoV-2, in ...Resources page for more information. Plasmids Expressing Spike and Spike Variants Full-length wildtype...many circulating variants. The table below lists expression plasmids that are suitable for generating pseudotyped...

  3. Retrovirus Plasmids

    Type
    Collection
    ... to eGFP expression Clevers 18760 MSCV IRES Luciferase MSCV Plasmid for transgene expression; also expresses...expresses luciferase Lowe 9044 pMIG MSCV Plasmid for transgene expression; also expresses GFP. Also see...CMV/MSV lncRNA expression plasmid Sun 60683 pLXIN-Luc MoMSV Stable expression of luciferase in mammalian...Ecotropic MLV expressing envelope plasmid Nienhuis and Salmon 35616 pCAG-VSVG Envelope VSV-G-expressing envelope...GFP MoMLV U6-driven plasmid for shRNA expression; also expresses GFP. See pMKO.1 puro for Puro resistance...27995 TtRMPVIR CMV/MSV Tet-regulated expression of shRNA; expresses rtTA. See Zuber et al. 2011 for additional... and infection results in long-term transgene expression. Some disadvantages of γ-retroviruses are that...

  4. Zhang Lab CRISPR Page

    Type
    Collection
    ...transfection : 61422 : Expresses dCAS9-VP64 activator with 2A GFP 61423 : Expresses the MS2-P65-HSF1 activator...Cas9 (SpCas9), using one vector to express SpCas9, and another to express up to three sgRNAs (Swiech et al...to LacZ, plus luciferase-2A-Cre recombinase 60226 : sgRNA cloning backbone with luciferase-2A-Cre recombinase...AAV-KPL) This plasmid contains two expression cassettes, Renilla luciferase-2A-Cre recombinase and sgRNAs ...-ITR This plasmid contains two expression cassettes, Renilla luciferase-2A-Cre recombinase and an sgRNA...nuclei in addition to sgRNA expression. This plasmid contains two expression cassettes, Cre recombinase...

  5. Empty Backbones - Choosing Your Perfect Plasmid Backbone

    Type
    Collection
    ... to express a gene; however, we have included a function section for if you want to stably express an ...genetic element, or want to express shRNA. Choose by: Species-Specific Expression Epitope Tag or Fusion Protein...Selectable Markers Species-Specific Expression If you want to drive expression of your favorite gene, you will...EF1a, CAG, Ubc Transient expression - Gradia Lab plasmids for mammalian expression, Ligation Independent ... Inducible Expression page Bacteria Lac, T7, araBAD, trp mTagBFP2-pBAD - Protein expression vector with...TEV cleavage Transient expression - Gradia Lab plasmids for bacterial expression, Ligation Independent ... Expression - Rubin lab plasmids for Drosophila expression pMT-puro - Inducible...

  6. Chemogenetics Plasmids

    Type
    Collection
    ...a scientist to control signaling within cells expressing these engineered receptors. Chemogenetic receptors... the specific cell type where the receptor is expressed, these receptors can be used to artificially activate...luminescent opsins: fusion proteins of a light-emitting luciferase and a light-sensing optogenetic element, making... bimodal opto-chemogenetic actuator. When the luciferase enzyme oxidizes its substrate (luciferin), it...

  7. Bacterial Expression Systems

    Type
    Collection
    ... to Top Controlled Expression It isn’t always beneficial to constitutively express your gene of interest... on expression only at a specific time. This collection has a variety of plasmids whose expression levels...can be found in pCS-PesaRlux controlling luciferase expression. pCW-LIC 26098 3x Tac promoter (hybrid of...studying gene expression in Gram-negative bacteria. pAK Series Various Plasmids pTac GFP, Luciferase (LuxCDABE...Attila Karsi Broad host range vectors for expressing luciferase and fluorescent protein reporter genes in... bacterial expression systems to find out more! Genome Engineering Although overexpressing or knocking...plasmids used for expression in bacterial systems Plasmid...

  8. Tetracycline Inducible Expression

    Type
    Collection
    ...Tetracycline (Tet) Inducible Expression Tetracycline (Tet) Inducible Expression Additional Resources: Browse...subsequent increase in expression by the activation domain, resulting in reduced gene expression. This idea of ... rtTA or TetR expression plasmid Stable cell lines can be made that continuously express a system component...Lentiviral, miRNA expression (PRIME) system for application in knockdown of gene expression at a single copy...copy in mammalian cells; Expresses firefly luciferase hairpin and GFP under pTREtight promoter None Either...promoters capable of controlling eukaryotic gene expression. Several endogenous promoters had been identified...coli presented the best candidates for inducible expression. Bacterial systems were tested for functionality...

  9. Plasmid Collections

    Type
    Collection
    ...Microbiology Plant Expression Stem Cells Synthetic Biology Tet Inducible Expression Worm Expression Kits Kits ...Zinc Fingers Luminescence Fluorescent Proteins Luciferase Optogenetics Chemogenetics Viral Plasmids Lentivirus... Topics Antibody Plasmid Collection Bacterial Expression Systems Empty Backbones Genetic Code Expansion...Collection Types CRISPR Fluorescent Proteins Protein Expression Synthetic Biology Viral Vectors Communities Find...

  10. Fluorescent Protein Guide

    Type
    Collection
    ... Resources You May Also Like... Luminescence: Luciferase Plasmids Blog: Which FP Should I Use? Blog: How... Proteins from Kurt Thorn and UCSF Molecular Expressions Galleries from Michael Davidson and FSU Spectra...

  11. Cre-lox system

    Type
    Collection
    ...present. By expressing Cre at specific times or locations, you can precisely control expression of your gene...developed ways to tightly control Cre expression and to optimize Cre expression once it is induced. Addgene has...defines the areas in which Cre will be expressed. Cre may be expressed globally under a broadly active promoter... or expressed only in a subset of cells under a more specific promoter (e.g. Rho-Cre is expressed in the...visualization of Cre expression. Optimized Cre: Codon-optimized Cre ( iCre ) is expressed at higher levels...pEFS-Rluc-2A-Cre-WPRE-hGHpA-ITR Cre, luciferase, and sgRNA expression EFS AAV Zhang 60229 AAV:ITR-U6-sgRNA... EGFP, and sgRNA expression hSyn AAV Zhang 60820 pSECC Cre, Cas9 and sgRNA expression EF-1 alpha Lentiviral...

  12. COVID-19 Resources

    Type
    Collection
    ...SARS-CoV-2 infection. General Tools : Plasmids expressing Cas12a/Cas13a, Reverse Transcriptases, etc. that...Collection - This page includes a table of all spike expression plasmids in our collection that are suitable ...spike pseudotyped virus. It also lists several luciferase and fluorescent reporter plasmids that have been...collection contains: Plasmids for E. coli and Yeast expression Spike protein constructs for making VSV pseudo...Taq polymerase and MMLV reverse transcriptase expression plasmids from the Drew Endy and Philippa Marrack...2020.04.13.039941 (Link opens in a new window) BST-LF expression plasmid from Andrew Ellington's lab. Find plasmid...

  13. Fluorescent Protein Guide: Biosensors

    Type
    Collection
    ...You may also like... Addgene Blog: Biosensors Luciferase Plasmids Subcellular Localization Optogenetics...Kim , GENIE Project Calcium Bicistronic vector expressing GCaMP6 and mRuby2 for imaging neural activity... Huebener , Tobias Rose Calcium Soma-targeted expression of jGCaMP8f, GCaMP7f, or GCaMP6f Precision Calcium...6. Edward Boyden Calcium Intersectional viral expression of GCaMP6m/s in cells depdent on Cre, Flp, or...Karl Deisseroth , INTRSECT 2.0 Project Calcium Expression of GCaMP6f in PV cortical interneurons under ... Aug 4;11(1):3881. Adam Cohen Calcium GCaMP6f expression in forebrain GABA-ergic interneurons under the... Oct 25;9(1):4440. Eric Schreiter Calcium AAV expression of photoconvertible fluorescent protein-based...

  14. Control AAV Preps

    Type
    Collection
    ...Service: Control AAV Browse our control AAV that express fluorescent proteins under general promoters. These...Constitutive 5, 8 Wilson 105532 pAAV.CMV.ffLuciferase.SV40 CMV ffLuciferase Constitutive 8 Wilson 105536 ...
Showing: 1 - 14 of 14 results