Skip to main content
Addgene

Enter a sequence to perform a BLAST-based search of Addgene plasmid sequence databases.

In Category

We narrowed to 15 results for: luciferase expression

Showing: 1 - 15 of 15 results

  1. Luciferase Plasmid Collection

    Type
    Collection
    ... Collections Luciferase Plasmids Luciferase Plasmid Collection Empty Backbones Expression Constructs Reporter... of luciferase found in Addgene's collection include: Firefly luciferase (Fluc) Renilla luciferase (Rluc...respectively), Cypridina luciferase , and Luciola luciferase . Each type of luciferase has advantages and disadvantages...within the luciferase gene allow for increased copy number in E. coli and expression of luciferase in the ...Groskreutz 18760 MSCV IRES Luciferase Firefly Retroviral expression of firefly luciferase and gene of interest...regions upstream of Firefly luciferase. Renilla luciferase is co-expressed. Oskar Laur 87070 pcDNA3.1-... regions upstream of Firefly luciferase. Renilla luciferase is expressed from a T7 promoter for normalization...

  2. Promega Plasmid Collection

    Type
    Collection
    ...NanoLuc luciferase-fusion protein expressed in cells. Additional Resources Browse Addgene's Luciferase Plasmid...Find luciferase reporter vectors for sensitive gene expression analysis and fusion vectors containing...The collection contains luciferase reporter vectors for sensitive gene expression analysis and protein fusion...HaloTag, SmBiT, LgBiT, HiBiT, and more. Luciferase Reporters Luciferase (Link opens in a new window) reporter...bright and stable luciferase that enables highly sensitive detection at endogenous expression levels. NanoLuc..., reporter, protein, or tag, such as "NFAT", "luciferase", "Ras", "NanoLuc", "HaloTag", "BiT", or other... from an enzymatic reaction to measure subtle expression changes with high sensitivity. These assays are...

  3. SARS-CoV-2 Pseudotyped Virus

    Type
    Collection
    ...reporter vector expressing firefly luciferase. pCMV-FLuc - Retroviral reporter vector expressing firefly luciferase... reporter vector expressing firefly luciferase and ZsGreen. NL4-3 mCherry Luciferase - Lentiviral dual...vector expressing destabilized EGFP. Lenti-luciferase-P2A-Neo - Lentiviral reporter vector expressing firefly...dual reporter vector expressing mCherry and firefly luciferase. pLentiEGFPdestablized - EFS-EGFPd2PEST-2A-MCS-Hygro...can then be used to infect permissive cells that express ACE2, the cellular receptor for SARS-CoV-2, in ...Resources page for more information. Plasmids Expressing Spike and Spike Variants Full-length wildtype...many circulating variants. The table below lists expression plasmids that are suitable for generating pseudotyped...

  4. Retrovirus Plasmids

    Type
    Collection
    ... to eGFP expression Clevers 18760 MSCV IRES Luciferase MSCV Plasmid for transgene expression; also expresses...expresses luciferase Lowe 9044 pMIG MSCV Plasmid for transgene expression; also expresses GFP. Also see...CMV/MSV lncRNA expression plasmid Sun 60683 pLXIN-Luc MoMSV Stable expression of luciferase in mammalian...Ecotropic MLV expressing envelope plasmid Nienhuis and Salmon 35616 pCAG-VSVG Envelope VSV-G-expressing envelope...GFP MoMLV U6-driven plasmid for shRNA expression; also expresses GFP. See pMKO.1 puro for Puro resistance...27995 TtRMPVIR CMV/MSV Tet-regulated expression of shRNA; expresses rtTA. See Zuber et al. 2011 for additional... and infection results in long-term transgene expression. Some disadvantages of γ-retroviruses are that...

  5. Zhang Lab CRISPR Page

    Type
    Collection
    ...transfection : 61422 : Expresses dCAS9-VP64 activator with 2A GFP 61423 : Expresses the MS2-P65-HSF1 activator...Cas9 (SpCas9), using one vector to express SpCas9, and another to express up to three sgRNAs (Swiech et al...to LacZ, plus luciferase-2A-Cre recombinase 60226 : sgRNA cloning backbone with luciferase-2A-Cre recombinase...AAV-KPL) This plasmid contains two expression cassettes, Renilla luciferase-2A-Cre recombinase and sgRNAs ...-ITR This plasmid contains two expression cassettes, Renilla luciferase-2A-Cre recombinase and an sgRNA...nuclei in addition to sgRNA expression. This plasmid contains two expression cassettes, Cre recombinase...

  6. Empty Backbones - Choosing Your Perfect Plasmid Backbone

    Type
    Collection
    ... to express a gene; however, we have included a function section for if you want to stably express an ...genetic element, or want to express shRNA. Choose by: Species-Specific Expression Epitope Tag or Fusion Protein...Selectable Markers Species-Specific Expression If you want to drive expression of your favorite gene, you will...EF1a, CAG, Ubc Transient expression - Gradia Lab plasmids for mammalian expression, Ligation Independent ...Empty mammalian expression plasmid for cloning untagged proteins pcDNA3.1-mCherry - Expresses mCherry tag ... Inducible Expression page Bacteria Lac, T7, araBAD, trp mTagBFP2-pBAD - Protein expression vector with...TEV cleavage Transient expression - Gradia Lab plasmids for bacterial expression, Ligation Independent ...

  7. Chemogenetics Plasmids

    Type
    Collection
    ...a scientist to control signaling within cells expressing these engineered receptors. Chemogenetic receptors... the specific cell type where the receptor is expressed, these receptors can be used to artificially activate...luminescent opsins: fusion proteins of a light-emitting luciferase and a light-sensing optogenetic element, making... bimodal opto-chemogenetic actuator. When the luciferase enzyme oxidizes its substrate (luciferin), it...

  8. Bacterial Expression Systems

    Type
    Collection
    ... Plasmid Collections Bacterial Expression Systems Bacterial Expression Systems Protein Purification Protein...bacterial expression systems. Read below and browse our curated collection of bacterial expression plasmids...Addgene Blog E. Coli Strains for Protein Expression Protein Expression Epitope Tags Protein Tags Return to ...to top Controlled Expression It isn’t always advantageous to constitutively express your gene of interest...turn on expression only at a specific time. This collection contains plasmids whose expression levels ...Addgene Controlled Expression Resources Check out our Tetracycline (Tet) Inducible Expression Collection for...transcription factors. Addgene Blog Luciferase Technologies Enabled by NanoLuc® Luciferase Fluorescent Biosensors ...

  9. Plasmid Collections

    Type
    Collection
    ...Microbiology Plant Expression Stem Cells Synthetic Biology Tet Inducible Expression Worm Expression Kits Kits ...Zinc Fingers Luminescence Fluorescent Proteins Luciferase Optogenetics Chemogenetics Viral Plasmids Lentivirus... Topics Antibody Plasmid Collection Bacterial Expression Systems Empty Backbones Genetic Code Expansion...Collection Types CRISPR Fluorescent Proteins Protein Expression Synthetic Biology Viral Vectors Communities Find...

  10. Fluorescent Protein Guide

    Type
    Collection
    ... Resources You May Also Like... Luminescence: Luciferase Plasmids Blog: Which FP Should I Use? Blog: How... Proteins from Kurt Thorn and UCSF Molecular Expressions Galleries from Michael Davidson and FSU Spectra...

  11. Cre-Lox and Other Site-Specific Recombinases

    Type
    Collection
    ...genome or control gene expression or skip ahead to browse highlighted plasmids expressing Cre , Flp , Dre ,...Recognition Sites. Controlling Recombinase Expression By expressing the enzyme at specific times or locations...popular recombinase-dependent gene expression strategies. Gene Expression: A stop codon flanked with loxP...return gene expression to physiological levels. In lox-STOP-lox shRNA constructs, Cre expression promotes...toxicity. Leakiness Leaky expression, where the gene of interest is expressed even in the absence of the...catalog for plasmids expressing Cre recombinase and filter the results by expression system and vector type...catalog for plasmids expressing Flp recombinase and filter the results by expression system and vector type...

  12. COVID-19 Resources

    Type
    Collection
    ...SARS-CoV-2 infection. General Tools : Plasmids expressing Cas12a/Cas13a, Reverse Transcriptases, etc. that...Collection - This page includes a table of all spike expression plasmids in our collection that are suitable ...spike pseudotyped virus. It also lists several luciferase and fluorescent reporter plasmids that have been...collection contains: Plasmids for E. coli and Yeast expression Spike protein constructs for making VSV pseudo...Taq polymerase and MMLV reverse transcriptase expression plasmids from the Drew Endy and Philippa Marrack...2020.04.13.039941 (Link opens in a new window) BST-LF expression plasmid from Andrew Ellington's lab. Find plasmid...

  13. Tetracycline Inducible Expression

    Type
    Collection
    ...Tetracycline (Tet) Inducible Expression Tetracycline (Tet) Inducible Expression You may also like... Blog...factors to further minimize background expression. Improved expression of the transactivator proteins in mammalian...Experimental Considerations Expression System If your gene of interest should only be expressed occasionally, use...PB-EF1a-TetOn3G PiggyBac vector expressing Tet-On 3G transactivator constitutively expressed by EF1a promoter Tet-On...Dox-inducible expression of Cre recombinase. Madeline Lancaster 198752 DiLiCre 2.0 Dox-inducible expression of a...Learn about tetracycline-regulated gene expression and find plasmids for your experiments using tet- ... promoters are powerful tools to control gene expression and study biological targets. The tetracycline...

  14. Fluorescent Protein Guide: Biosensors

    Type
    Collection
    ...You may also like... Addgene Blog: Biosensors Luciferase Plasmids Subcellular Localization Optogenetics...Kim , GENIE Project Calcium Bicistronic vector expressing GCaMP6 and mRuby2 for imaging neural activity... Huebener , Tobias Rose Calcium Soma-targeted expression of jGCaMP8f, GCaMP7f, or GCaMP6f Precision Calcium...6. Edward Boyden Calcium Intersectional viral expression of GCaMP6m/s in cells depdent on Cre, Flp, or...Karl Deisseroth , INTRSECT 2.0 Project Calcium Expression of GCaMP6f in PV cortical interneurons under ... Aug 4;11(1):3881. Adam Cohen Calcium GCaMP6f expression in forebrain GABA-ergic interneurons under the... Oct 25;9(1):4440. Eric Schreiter Calcium AAV expression of photoconvertible fluorescent protein-based...

  15. Control AAV Preps

    Type
    Collection
    ...Service: Control AAV Browse our control AAV that express fluorescent proteins under general promoters. These...Constitutive 5, 8 Wilson 105532 pAAV.CMV.ffLuciferase.SV40 CMV ffLuciferase Constitutive 8 Wilson 105536 ...
Showing: 1 - 15 of 15 results