We narrowed to 15 results for: puro
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TypeCollection...)-2A-Puro (PX462) V2.0 62987 Mammalian BbsI yes, nick S. pyogenes Puro Zhang pSpCas9(BB)-2A-Puro (PX459...pyogenes Puro, TagBFP Yusa lentiGuide-Puro 52963 Mammalian/Lentiviral BsmBI none S. pyogenes Puro Zhang ...pyogenes Puro Moffat lenti sgRNA(MS2)_puro backbone 73795 Mammalian/Lentiviral U6 none S. pyogenes Puro Zhang...Ebert pLKO.1-puro U6 sgRNA BfuAI stuffer 50920 Mammalian/Lentiviral BfuAI none S. pyogenes Puro Wolfe pL-...pyogenes Puro Ebert pL-CRISPR.EFS.PAC 57828 Mammalian/Lentiviral BsmBI yes, cut S. pyogenes Puro Ebert pL-CRISPR.SFFV.PAC...pLenti SpBsmBI sgRNA Puro 62207 Mammalian/Lentiviral BsmBI none S. pyogenes Puro Maehr pKLV-U6gRNA-EF(...pyogenes Fire pGL3-U6-sgRNA-PGK-Puro 51133 Mammalian none S. pyogenes Puro Huang pAC152-dual-dCas9VP64-sgExpression...
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Kazuhiro Oka Lentiviral Vectors
TypeCollection...pCDH-CB-iCre-P2A-tdTomato-T2A-Puro 72255 Cre is coexpressed with tdTomato and Pac (puromycin N-acethyl-transferase...pCDH-CB-FLPe-P2A-copGFP-T2A-Puro 72258 Expresses FLPe, copGFP and the puromycin resistance from the CB promoter...promoter pCDH-EF1-copGFP-T2A-Puro 72263 Express copGFP and the puromycin resistance gene from the EF1 ... promoter pCDH-CMV-mCherry-T2A-Puro 72264 Express mCherry and puromycin resistance gene from the CMV promoter...increase the cloning capacity. pCDH-CB-IRES-copGFP-T2A-Puro 72299 Express gene of interest from the CB promoter...the mouse U6 promoter pCDH-EF1-Nluc-P2A-copGFP-T2A-Puro 73022 Expresses Nluc and copGFP from the EF1 promoter...from the EF1 promoter pCDH-EF1s-Nluc-P2A-copGFP-T2A-Puro 73032 Expresses Nluc and copGFP from a truncated... -
Lentivirus Plasmids
TypeCollection...Description PI 8453 pLKO.1 puro 3rd U6-driven shRNA empty plasmid with puromycin selection. See plasmid 24150...Diane Mathis 21915 Tet-pLKO-puro 3rd Inducible expression of shRNA with puromycin selection. See plasmid 21916...Eric Campeau 39481 pLenti-puro 3rd CMV-driven expression of cDNA with puromycin selection. Ie-Ming Shih ...pBOB-EF1-FastFUCCI-Puro 3rd Encodes FUCCI reporters for cell cycle analysis. Puromycin selection. Kevin ...17448 pLenti CMV GFP Puro (658-5) 3rd eGFP expression with CMV promoter and puromycin selection. See Campeau...for GFP reporter. Linzhao Cheng 17452 pLenti CMV Puro DEST 3rd Gateway destination plasmid with constitutive...additional variants. David Sabatini 171123 pLVX-TetOne-Puro-GFP 3rd Dox-inducible expression of GFP. Jason Sheltzer... -
Lentiviral Prep Service
TypeCollection...library Brunello in lentiGuide-Puro Human sgRNA library in backbone lentiGuide-Puro targeting 19,114 genes and...library Brie in lentiGuide-Puro Mouse sgRNA library in backbone lentiGuide-Puro containing 78,637 unique... genes along with 1000 non-targeting controls Puromycin Doench , Root 73179 Human gRNA pooled library ... genes along with 1000 non-targeting controls Puromycin Doench , Root Human activation pooled libraries...56,762 unique sgRNAs targeting 18,885 human genes Puromycin Doench , Root Human inactivation pooled libraries...human genes along with 500 non-targeting controls Puromycin Doench , Root 92386 (Discontinued) Human CRISPR...human genes along with 500 non-targeting controls Puromycin Doench , Root Mouse knockout pooled libraries ... -
Retrovirus Plasmids
TypeCollection...pMSCV PIG (Puro IRES GFP empty plasmid) MSCV For cloning and gene expression. Select with puromycin or screen...47916 pRXTN CMV/MSV A modified version of pRX-tight Puro encoding NAT instead of PAC. James Iglehart 20672...GFP. David Bartel 32702 pMSCV-loxp-dsRed-loxp-eGFP-Puro-WPRE MSCV Conditional overexpression plasmid. Deletion...creation of immortalized cells. See plasmid 1771 for puromycin selection, and plasmid 1774 for neomycin selection...expression with GFP expression. See plasmid 8452 for puromycin resistance. William Hahn 63704 pRetroX GFP T2A... -
Tetracycline Inducible Expression
TypeCollection...21915 Tet-pLKO-puro Lentiviral Tet-On plasmid for inducible expression of shRNA with puromycin selection. ...TetR H1-2O2 Dmitri Wiederschain 85966 EZ-Tet-pLKO-Puro Lentiviral Tet-On plasmid for easier cloning and... TetR H1-2O2 Cindy Miranti 104321 tet-pLKO-sgRNA-puro Lentiviral Tet-On plasmid for inducible expression...Tet-On 3G rtTA TRE3GS Xiaojun Lian 171123 pLVX-TetOne-Puro-GFP Lentiviral Tet-On vector for inducible expression...for CRISPRa Emma Rawlins 72835 pMK243 (Tet-OsTIR1-PURO) Expresses OsTIR1 under the control of a TRE3GS ...and design of inducible shRNA expression, with puromycin selection. See Plasmid #85973 for blasticidin ...inducible expression; insert with Gateway cloning and puromycin selection rtTA-Advanced Tight TRE David Root 100521... -
Empty Backbones - Choosing Your Perfect Plasmid Backbone
TypeCollection...selection Puromycin Mammalian pcDNA3.1-(-)-Pur - Eukaryotic expression vector Tet-pLKO-puro - ...Ligation Independent Cloning (LIC) pAG CMV Empty Puro - Empty mammalian expression plasmid for cloning...plasmids for Drosophila expression pMT-puro - Inducible gene expression under...lentiviral vector for mammalian genome editing Tet-pLKO-puro and Tet-pLKO-neo - Tet-inducible lentiviral shRNA...mCherry selectable marker pMSCV-U6sgRNA(BbsI)-PGKpuro2ABFP - For retroviral delivery of one sgRNA pMKO.1...lentiviral vector for EGFP fusion; PGK driven puromycin Hygromycin Mammalian, Varies pLKO.1 hygro - Lentiviral... -
Neurodegeneration Plasmid Collection
TypeCollection...115182 pLD-puro-Cc-PARK7WT-VA PARK7 His, Flag, Streptactin CMV Parkinson's Mohan Babu 115183 pLD-puro-Cc-PARK7V51G-VA... pLD-puro-Cc-PARK7C53A-VA PARK7 His, Flag, Streptactin CMV Parkinson's Mohan Babu 115185 pLD-puro-Cc-PARK7H126A-VA...pLD-puro-Cc-PARK7E163K-VA PARK7 His, Flag, Streptactin CMV Parkinson's Mohan Babu 115187 pLD-puro-Cc-CR-PARK7WT-VA...Roussel 133753 pLVX-Puro-TDP-43-WT TARDBP CMV ALS Shawn Ferguson 133754 pLVX-Puro-TDP-43-A90V TARDBP CMV...Ferguson 133755 pLVX-Puro-TDP-43-A315T TARDBP CMV ALS Shawn Ferguson 133756 pLVX-Puro-TDP-43-A382T TARDBP...Ferguson 133757 pLVX-Puro-TDP-43-D169G TARDBP CMV ALS Shawn Ferguson 133758 pLVX-Puro-TDP-43-K263E TARDBP...Ferguson 133759 pLVX-Puro-TDP-43-M337V TARDBP CMV ALS Shawn Ferguson 133760 pLVX-Puro-TDP-43-P112H TARDBP... -
Zhang Lab CRISPR Page
TypeCollection...targeting efficiency. SpCas9 and SpCas9n with 2a-Puro and 2a-EGFP are also available. 2. SpCas9 (or SpCas9n...2A-EGFP and single guide RNA 62988 : PX459; SpCas9-2A-Puro and single guide RNA 48873 : PX460; SpCas9n (D10A...) and single guide RNA 62987 : PX462; SpCas9n-2A-Puro (D10A nickase) and single guide RNA 48137 : PX165...2 vector system - lentiCas9-Blast and lentiGuide-Puro (sgRNA) Browse the Addgene GeCKO Library CRISPR ... -
Luciferase Plasmid Collection
TypeCollection.... Lentiviral Nadav Ahituv 212935 pGL4.84(hRlucCP/Puro) RapidResponse™ Renilla Vector for investigating...controlling transcription Pete Stecha 212933 pGL4.82(hRluc/Puro) Renilla Vector for investigating regions controlling...luciferase Mark Kay 140328 pLenti-PGK-Venus-Fluc (puro) Firefly hPGK Lentiviral expression of Venus-firefly...firefly luciferase Christopher Vakoc 33307 Ubc.Luc.IRES.Puro Firefly Ubc Lentiviral expression of firefly...Venus-Aka-luciferase Roland Friedel 102320 FUW-RLuc-T2A-PuroR Renilla Ubc Lentiviral Expression of Renilla luciferase... -
CRISPR Plasmids - Prime Edit
TypeCollection...Weber 173220 pPBT-peRNA_GG-Puro Mammalian, piggyBac hU6 pegRNA BsmBI No Puromycin Jacob Giehm Mikkelsen 173222...pPBT-PE2-PuroTK-pegRNA_GG Mammalian, piggyBac hU6 pegRNA BsmBI Yes (Cas9 H840A + MMLV RT) PuroTK Jacob Giehm... -
CRISPR References and Information
TypeCollection... 2 vector system: lentiCas9-Blast and lentiGuide-Puro packaging plasmids: pVSVg , psPAX2 positive control... -
Fluorescent Protein Guide: Subcellular Localization
TypeCollection...Autophagosome LC3(ATG8) mCherry Maria Harrison 38277 pMXs-puro GFP-p62 Autophagosome Sequestosome-1 EGFP Noboru...Chromatin H2B mRFP Bryan Welm, Zena Werb 21045 pH2B_mCherry_IRES_puro2 Chromatin H2B mCherry Daniel Gerlich 25999... -
CRISPR Plasmids - Tagging
TypeCollection...system plasmids for CRISPR-based knock-in of EGFP-2A-PuroR cassette to the C-terminus of endogenous proteins...vector. The PITCh donor plasmid with an EGFP-2A-PuroR cassette, flanked by microhomologous sequences and...deposited PITCh plasmids were tested by fusing EGFP-2A-PuroR cassette to a nucleolar protein, fibrillarin (FBL...pCRIS-PITChv2-FBL - PITCh donor vector for EGFP-2A-PuroR knock-in into human FBL locus Jorgensen Lab SapTrap... -
CRISPR Plasmids - Drosophila
TypeCollection...Port 49330 pAc-sgRNA-Cas9 dU6 BspQI Transfection Puromycin yes, cut Ji-Long Liu 45946 pU6-BbsI-chiRNA dU6...