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Enter a sequence to perform a BLAST-based search of Addgene plasmid sequence databases.

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We narrowed to 15 results for: sgrna vector

Showing: 1 - 15 of 15 results

  1. Zhang Lab CRISPR Page

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    Collection
    ... in two formats: 1 vector system - lentiCRISPR - sgRNA and SpCas9 together 2 vector system - lentiCas9...described here. #60224 - AAV:ITR-U6-sgRNA(Kras)-U6-sgRNA(p53)-U6-sgRNA(Lkb1)-pEFS-Rluc-2A-Cre-shortPA-KrasG12D_HDRdonor-ITR...below) can be cloned scarlessly into the vector before the sgRNA scaffold. The oligos are designed based...lentiviral vector encoding dCAS9-VP64 with 2A Blast resistance marker 61426 : lentiviral vector encoding... both single- and dual-vector systems that allow the delivery of Cas9 and sgRNA in vivo via adeno-associated...single guide RNA (sgRNA) into one plasmid (Ran et al., Nature 2015). The dual vector system uses S. pyogenes...SpCas9), using one vector to express SpCas9, and another to express up to three sgRNAs (Swiech et al., Nature...

  2. Lentiviral Prep Service

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    ...more details about our viral vector packaging service, visit our Viral Vector Packaging Service page . Pooled...pooled libraries 73178 Human sgRNA library Brunello in lentiGuide-Puro Human sgRNA library in backbone lentiGuide-Puro...Human CRISPRa sgRNA library Calabrese in backbone XPR_502 (P65 HSF), Set A Human CRISPRa sgRNA activation...containing 56,762 unique sgRNAs. This backbone does not contain dCas9. 56,762 unique sgRNAs targeting 18,885 ...pooled libraries 73633 Mouse sgRNA library Brie in lentiGuide-Puro Mouse sgRNA library in backbone lentiGuide-Puro...Ready-to-use lentivirus available from Addgene's viral vector packaging service. Browse constructs currently ... Viral Vector Packaging Service Lentivirus Browse In-Stock Lentivirus...

  3. CRISPR Plasmids - Empty gRNA Vectors

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    ... Empty Vectors Many gRNA empty vectors have been deposited at Addgene. To find the gRNA vector for your... and allow for expression of more sgRNAs in a single vector. sgRNAs are expressed as polycistronic glycine...Mammalian BbsI none S. pyogenes Gersbach pUC57-sgRNA expression vector 51132 Mammalian BsaI none S. pyogenes Huang...pyogenes Joung sgRNA with U6 promoter 48962 Worm HindIII none S. pyogenes de Bono sgRNA with rpr-1 promoter...SpBsmBI sgRNA Hygro 62205 Mammalian/Lentiviral BsmBI none S. pyogenes Hygro Maehr pLenti SpBsmBI sgRNA Puro...Bacteria T7 yes, cut N. meningitidis Joung Cas9 sgRNA vector 68463 Mammalian U6 none S. pyogenes Zhang Cas9...Multiplex gRNA Vectors Several systems have been developed to generate multiplex gRNA vectors. These systems...

  4. CRISPR Plasmids - Prime Edit

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    ...Publication Empty Prime Editing gRNA Vectors A selection of empty gRNA vectors suitable for prime editing are...nicking sgRNA BbsI No Vermilion Norbert Perrimon 164423 pHSG1C3 Mammalian U6 pegRNA + nicking sgRNA BbsI ... RNA-H1-nick sgRNA-mCherry Mammalian, AAV hU6 + H1 pegRNA + nicking sgRNA No mCherry Hyongbum ...HindIII No Tilmann Weber 172717 pnsgRNA Bacteria J23119 (BBa_J23119) nicking sgRNA No Tilmann Weber 173220 pPBT-peRNA_GG-Puro...-FokI Screen Pooled Libraries gRNAs Empty gRNA Vectors gRNAs By Species Mammalian Bacteria Drosophila ...mutations in RT enzyme PE3 - PE2 plus additional sgRNA PE4 - PE2 plus MLH1 (a protein component of MMR)... table below. Use the search bar to find a gRNA vector based on expression system, promoter, the type ...

  5. CRISPR References and Information

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    ... gRNA design and cloning gRNA cloning vector Retroviral vectors: neomycin (pSIR-neo) , GFP (pSIR-GFP) ... Sabatini and Lander gRNA cloning into pLX-sgRNA pLX-sgRNA PDF, 129 KB Sontheimer gRNA design and cloning... pooled library amplification 1 vector system: lentiCRISPR v2 2 vector system: lentiCas9-Blast and lentiGuide-Puro... can identify positively or negatively selected sgRNAs, genes, or pathways. For use with genome-scale ... opens in a new window) for lentiviral screens. sgRNA Scorer 2.0 (Link opens in a new window) A tool that... and a ctivation (Link opens in a new window) A sgRNA design tool for genome editing, as well as gene ...Link opens in a new window) Identifies candidate sgRNA target sites by off-target quality. Validated for...

  6. Genomic Deletions in Mammalian Cell Lines

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    ...example, sgRNA-A’s protospacer sequence and PAM happen to fall on the bottom (Crick) strand while sgRNA-B’s... the 20-mer is not G. sgRNA expression from the U6 promoter of the pX330 vector is enhanced by the inclusion...primers flanking each sgRNA target site (within 150 - 350 bp) to amplify the sgRNA target site to examine...complement: 1.0 μl sgRNA 24- or 25-mer oligo (100 μM; see step 1.4 ), 1.0 μl sgRNA 24- or 25-mer reverse...composed of a polyclonal population exposed to sgRNA-A and sgRNA-B (see step 5 ). The purpose of the following...delivery of a pair of chimeric single guide RNAs (sgRNAs) to create two double strand breaks (DSBs) at a...posted at the top of the page. CRISPR Design Design sgRNAs manually or using freely available online tools...

  7. CRISPR Pooled gRNA Libraries

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    ...46,354 BARBEKO sgRNA Library 174163 Knockout, Base Editing Human Wei 3rd 3 53,502 sgRNA Pool for Streptococcus...-FokI Screen Pooled Libraries gRNAs Empty gRNA Vectors gRNAs By Species Mammalian Bacteria Drosophila ...131625 Inhibition E. coli Bikard N/A ∼5 21,417 Bison sgRNA Library 169942 Knockout Human Ebert 3rd 4 2,852 ...Fly Perrimon 3rd 8 6 6 7,956 17,827 59,406 Dual sgRNA CRISPRi Libraries 187246 — 187248 1000000192 Inhibition...Inhibition Human Weissman 3rd 6 Varies Dual sgRNA CRISPRa Libraries 187249 — 187251 1000000193 Activation...3rd 2 37,722 Green monkey (Chlorocebus sabaeus) sgRNA library 178284 Knockout Green Monkey Doench and ...Knockout Human Parts 3rd 3 59,576 Human Whole Genome sgRNA iBAR Library 140633 Knockout Human Wei 3rd 3 58,...

  8. Empty Backbones - Choosing Your Perfect Plasmid Backbone

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    ...compatible Y2H vector - BD pRPR1_gRNA_handle_RPR1t - gRNA expression empty vector pFA6a vectors - Epitope ...pFastBac Dual LIC - Vectors from the Gradia lab pFB-LIC-Bse - Insect expression vector from Gileadi lab ...expression vector with N- or C-terminal HA tag pInducer20 - Tet-inducible HA-tagged lentiviral vector for ORF...Gateway destination vector for bacterial expression; His,GST tag pE2c - Modified pENTR vector for C-terminal...-1-3xMyc - Bacterial vector for Myc tag pETcon(-) - Yeast surface display vector with a C-terminal Myc...His-tagged versions of pFastBac LIC vectors and pFastBac Dual LIC vectors for insect expression Fluorescent...35S-eGFP-nosT - Transient expression vector for eGFP in plants Find FP tagging vectors in budding yeast pHT101-mCherry...

  9. CRISPR Plasmids - C. elegans

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    Collection
    ...-FokI Screen Pooled Libraries gRNAs Empty gRNA Vectors gRNAs By Species Mammalian Bacteria Drosophila ... Promoter PI Publication Empty gRNA Expression Vectors Select a gRNA expression plasmid based on factors...both the gRNA and Cas protein act as all-in-one vectors, but their function is often limited to a single... (PAM = NGG) 47549 pDD162 (Peft-3::Cas9 + Empty sgRNA) R07E5.16 U6 yes, cut Goldstein 42250 DR274 T7 BsaI...transcription none, need Cas9 plasmid Boxem 47912 SP6-sgRNA-scaffold SP6 AflII In vitro transcription none, ...need Cas9 plasmid Sternberg 177783 L4440_BioBrick-sgRNA T7 BbsI, BsaI Ingestion by C. elegans none, need...transcription none, need Cas9 plasmid Joung 46170 PU6::klp-12_sgRNA cU6 PCR (see paper) Transfection none, need Cas9...

  10. CRISPR Plasmids - Drosophila

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    ...-FokI Screen Pooled Libraries gRNAs Empty gRNA Vectors gRNAs By Species Mammalian Bacteria Drosophila ... Promoter PI Publication Empty gRNA Expression Vectors Select a gRNA expression plasmid based on factors...both the gRNA and Cas protein act as all-in-one vectors, but their function is often limited to a single...none, need Cas9 plasmid Bullock and Port 49330 pAc-sgRNA-Cas9 dU6 BspQI Transfection Puromycin yes, cut Ji-Long...

  11. Tetracycline Inducible Expression

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    Collection
    ...plasmid. A single vector approach may not have as much flexibility and control as a dual vector design, but ...popular empty backbones, expression vectors for transactivators, and vectors for other applications, or search...104321 tet-pLKO-sgRNA-puro Lentiviral Tet-On plasmid for inducible expression of sgRNA TetR H1-2O2 Nathanael...Lentiviral Tet-Off vector for repressible expression with blasticidin selection. *Note: this vector contains an...respectively. You should also choose between: Dual vector system: express the transactivator and the tet-...-controlled gene of interest from two separate vectors. Stable cell lines or transgenic animals can be...available for many transactivator or tet-inducible vectors. Tetracycline-inducible Backbones Find backbones...

  12. CRISPR Plasmids - Tagging

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    ...targeting vector assembly, and the remaining 5 plasmids include Cre and FLP expression vectors, a general...-FokI Screen Pooled Libraries gRNAs Empty gRNA Vectors gRNAs By Species Mammalian Bacteria Drosophila ...Cas plasmid-system consists of two components: A vector containing Cas9 and a validated gRNA, based on ...the isolation of native protein complexes. This vector serves as a backbone to clone the left and right...Alternatively, cDNAs can be cloned directly into this vector and targeted to the AAVS1 genomic safe harbor locus...gRNA_AAVS1-T2 (Addgene #41818) or using an all in one vector from the Doyon lab, eSpCas9(1.1)_No_FLAG_AAVS1_...of HDR, which makes it easy to construct a donor vector, because MMEJ utilizes very short (around 20 bp...

  13. CRISPR Guide

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    ...format into lentiviral transfer vectors (Figure 8B). Libraries come in 1-vector systems, in which Cas9 is included...systems contain two components: a guide RNA ( gRNA or sgRNA ) and a CRISPR-associated endonuclease (Cas enzyme...targeting in the double digits. These multiplex gRNA vectors can be used to knock out, activate, or repress ...multiplexing . Browse Plasmids: Multiplex gRNA Vectors Engineering the Cas9 Endonuclease CRISPR specificity...using a prime editing guide RNA ( pegRNA ). Like sgRNA, pegRNA directs the nickase to the target site by...efficiency PE3 - increased efficiency; an additional sgRNA directs Cas9n to a nearby site, driving mismatch...heterogeneous population of lentiviral transfer vectors. In most cases, each plasmid contains an individual...

  14. Luciferase Plasmid Collection

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    ...cells Peter Cockerill 212936 pGL3 Basic Vector Firefly Vector for investigating regions controlling transcription...Scott Lowe 21375 pHIV-Luciferase Firefly Lentiviral vector for insertion of your gene of interest upstream...and renilla_polyA Firefly, Renilla Dual reporter vector. Insertion of 5' promoter/enhancer regions upstream...212935 pGL4.84(hRlucCP/Puro) RapidResponse™ Renilla Vector for investigating regions controlling transcription... Pete Stecha 212933 pGL4.82(hRluc/Puro) Renilla Vector for investigating regions controlling transcription...pGL4Luc-RLuc Firefly, Renilla Dual reporter reporter vector allowing the study of biscistronic promoters. Daniel...119760 pSGDlucV3.0 Firefly, Renilla Dual reporter vector. Insertion of 5' promoter/enhancer regions upstream...

  15. Neurodegeneration Plasmid Collection

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    ...Walker Jackson 78624 sgRNA(MS2)_Prnp_SAM1 PRNP U6 Dementia Walker Jackson 78625 sgRNA(MS2)_Prnp_SAM2 PRNP...19 James Trimmer 129409 Slc1a3-CreERT2 Targeting Vector SLC1A3 PGK Episodic ataxia Walker Jackson 129410...His T5 Parkinson's Suzanne Pfeffer 208392 Lenti-sgRNA-Cre-GpNLuc-sgmATM ATM Ataxia telangiectasia Gaorav...TARDBP-Exon1-ko-sgRNA1-SpCas9 TARDBP U6 ALS Rajat Rohatgi 107858 pHBS953 TARDBP-Exon2-ko-sgRNA2-SpCas9 TARDBP...133762 px459-TDP-43 sgRNA1 TARDBP U6 ALS Shawn Ferguson 133763 px459-TDP 43 sgRNA2 TARDBP U6 ALS Shawn...TIA1 His, T7 T7 ALS Thomas Tuschl 106108 px458_2A_GFP_sgRNA_EIF2AK2 EIF2AK2 U6 VWM disease Thomas Tuschl ...Episodic ataxia Xiaolong Wang 159788 pGL3-U6-sgRNA_KCNA1-mcherry KCNA1 U6 Episodic ataxia Xiaolong Wang...
Showing: 1 - 15 of 15 results