We narrowed to 13 results for: sgrna vector

... in two formats: 1 vector system - lentiCRISPR - sgRNA and SpCas9 together 2 vector system - lentiCas9...described here. #60224 - AAV:ITR-U6-sgRNA(Kras)-U6-sgRNA(p53)-U6-sgRNA(Lkb1)-pEFS-Rluc-2A-Cre-shortPA-KrasG12D_HDRdonor-ITR...below) can be cloned scarlessly into the vector before the sgRNA scaffold. The oligos are designed based...lentiviral vector encoding dCAS9-VP64 with 2A Blast resistance marker 61426 : lentiviral vector encoding... both single- and dual-vector systems that allow the delivery of Cas9 and sgRNA in vivo via adeno-associated...single guide RNA (sgRNA) into one plasmid (Ran et al., Nature 2015). The dual vector system uses S. pyogenes...SpCas9), using one vector to express SpCas9, and another to express up to three sgRNAs (Swiech et al., Nature...

...more details about our viral vector packaging service, visit our Viral Vector Packaging Service page . Pooled...pooled libraries 73178 Human sgRNA library Brunello in lentiGuide-Puro Human sgRNA library in backbone lentiGuide-Puro...Human CRISPRa sgRNA library Calabrese in backbone XPR_502 (P65 HSF), Set A Human CRISPRa sgRNA activation...containing 56,762 unique sgRNAs. This backbone does not contain dCas9. 56,762 unique sgRNAs targeting 18,885 ...pooled libraries 73633 Mouse sgRNA library Brie in lentiGuide-Puro Mouse sgRNA library in backbone lentiGuide-Puro...Ready-to-use lentivirus available from Addgene's viral vector packaging service. Browse constructs currently ... Viral Vector Packaging Service Lentivirus Browse In-Stock Lentivirus...

... top Empty Prime Editing gRNA Vectors A selection of empty gRNA vectors suitable for prime editing are...nicking sgRNA BbsI No Vermilion Norbert Perrimon 164423 pHSG1C3 Mammalian U6 pegRNA + nicking sgRNA BbsI ... RNA-H1-nick sgRNA-mCherry Mammalian, AAV hU6 + H1 pegRNA + nicking sgRNA No mCherry Hyongbum ...HindIII No Tilmann Weber 172717 pnsgRNA Bacteria J23119 (BBa_J23119) nicking sgRNA No Tilmann Weber 173220 pPBT-peRNA_GG-Puro...mutations in RT enzyme PE3 — PE2 plus additional sgRNA PE4 — PE2 plus MLH1 (a protein component of MMR)... table below. Use the search bar to find a gRNA vector based on expression system, promoter, the type ...type of gRNA (e.g., pegRNA, epegRNA, nicking sgRNA), cloning enzyme, selectable marker, and whether the plasmid...

... gRNA design and cloning gRNA cloning vector Retroviral vectors: neomycin (pSIR-neo) , GFP (pSIR-GFP) ... Sabatini and Lander gRNA cloning into pLX-sgRNA pLX-sgRNA PDF, 129 KB Sontheimer gRNA design and cloning... pooled library amplification 1 vector system: lentiCRISPR v2 2 vector system: lentiCas9-Blast and lentiGuide-Puro... can identify positively or negatively selected sgRNAs, genes, or pathways. For use with genome-scale ... opens in a new window) for lentiviral screens. sgRNA Scorer 2.0 (Link opens in a new window) A tool that... and a ctivation (Link opens in a new window) A sgRNA design tool for genome editing, as well as gene ...Link opens in a new window) Identifies candidate sgRNA target sites by off-target quality. Validated for...

...compatible Y2H vector - BD pRPR1_gRNA_handle_RPR1t - gRNA expression empty vector pFA6a vectors - Epitope ...pFastBac Dual LIC - Vectors from the Gradia lab pFB-LIC-Bse - Insect expression vector from Gileadi lab ...expression vector with N-terminal HA tag pInducer20 - Tet-inducible HA-tagged lentiviral vector for ORF ...Gateway destination vector for bacterial expression; His,GST tag pE2c - Modified pENTR vector for C-terminal...-1-3xMyc - Bacterial vector for Myc tag pETcon(-) - Yeast surface display vector with a C-terminal Myc...His-tagged versions of pFastBac LIC vectors and pFastBac Dual LIC vectors for insect expression Fluorescent...35S-eGFP-nosT - Transient expression vector for eGFP in plants Find FP tagging vectors in budding yeast pHT101-mCherry...

... Resources Gamma-Retroviral Vector Guide All Viral Plasmids Viral Vectors 101 eBook Biosafety Resource...gamma-retroviruses have been engineered into gamma-retroviral vectors for safe use in the lab. The gamma-retrovirus components... safety: Transfer plasmid — contains transgene, sgRNA, or shRNA of interest flanked by LTRs Packaging ...and envelope plasmids. Read our Gamma-retroviral Vector Guide for more information about gamma-retroviral... production, and viral safety. Gamma-Retroviral Vector Guide Popular Gamma-Retroviral Transfer Plasmids...

...plasmid. A single vector approach may not have as much flexibility and control as a dual vector design, but ...popular empty backbones, expression vectors for transactivators, and vectors for other applications, or search...104321 tet-pLKO-sgRNA-puro Lentiviral Tet-On plasmid for inducible expression of sgRNA TetR H1-2O2 Nathanael...Lentiviral Tet-Off vector for repressible expression with blasticidin selection. *Note: this vector contains an...respectively. You should also choose between: Dual vector system: express the transactivator and the tet-...-controlled gene of interest from two separate vectors. Stable cell lines or transgenic animals can be...available for many transactivator or tet-inducible vectors. Tetracycline-inducible Backbones Find backbones...

...Resources Improved Gateway binary vectors — High-performance vectors for creation of fusion constructs...expression. Cloning and Synthetic Biology Expression Vectors The table below highlights plasmids that can be...collections are designed for creating plant expression vectors. ID Kit Description Cloning Type PI 1000000036 ...two-hybrid and bacterium-to-plant translocation vectors. Partially compatible with GoldenBraid. Golden ...functional parts for assembling plant transformation vectors, compatible with MoClo. Golden Gate Daniel Voytas...synthetic operons into chloroplast transformation vectors. Golden Gate (MoClo) Scott Lenaghan 1000000241 ... Kit Modular assembly of multi-gene expression vectors. Golden Gate (GreenGate) Wayne Parrott Additional...

...targeting vector assembly, and the remaining 5 plasmids include Cre and FLP expression vectors, a general...-FokI Screen Pooled Libraries gRNAs Empty gRNA Vectors By Species Mammalian Bacteria Drosophila Plant ...Cas plasmid-system consists of two components: A vector containing Cas9 and a validated gRNA, based on ...the isolation of native protein complexes. This vector serves as a backbone to clone the left and right...Alternatively, cDNAs can be cloned directly into this vector and targeted to the AAVS1 genomic safe harbor locus...gRNA_AAVS1-T2 (Addgene #41818) or using an all in one vector from the Doyon lab, eSpCas9(1.1)_No_FLAG_AAVS1_...of HDR, which makes it easy to construct a donor vector, because MMEJ utilizes very short (around 20 bp...

...engineered into viral vectors for safe use in the lab. One safety feature of lentiviral vectors is that the components...plasmids: Transfer plasmid — contains transgene, sgRNA, or shRNA of interest flanked by LTRs Packaging ...plasmids available at Addgene. Read our Lentiviral Vector Guide for more information about lentiviral components... viral production, and viral safety. Lentiviral Vector Guide Popular Lentiviral Transfer Plasmids Looking...Description PI 1732 pCPRDEnv 2nd Part of the FELIX vector system. Expresses gag-pol and rev . Garry Nolan...Kasparov Return to top Additional Resources Lentiviral Vector Guide Addgene’s Viral Service Lentiviral Pooled...Pooled Libraries All Viral Plasmids Viral Vectors 101 eBook Biosafety Resource Guide Addgene blog: Tips for...

...format into lentiviral transfer vectors (Figure 8B). Libraries come in 1-vector systems, in which Cas9 is included...systems contain two components: a guide RNA ( gRNA or sgRNA ) and a CRISPR-associated endonuclease (Cas enzyme...targeting in the double digits. These multiplex gRNA vectors can be used to knock out, activate, or repress ...multiplexing . Browse Plasmids: Multiplex gRNA Vectors Engineering the Cas9 Endonuclease CRISPR specificity...using a prime editing guide RNA ( pegRNA ). Like sgRNA, pegRNA directs the nickase to the target site by...efficiency PE3 — increased efficiency; an additional sgRNA directs Cas9n to a nearby site, driving mismatch...heterogeneous population of lentiviral transfer vectors. In most cases, each plasmid contains an individual...

...cells Peter Cockerill 212936 pGL3 Basic Vector Firefly Vector for investigating regions controlling transcription...Scott Lowe 21375 pHIV-Luciferase Firefly Lentiviral vector for insertion of your gene of interest upstream...and renilla_polyA Firefly, Renilla Dual reporter vector. Insertion of 5' promoter/enhancer regions upstream...212935 pGL4.84(hRlucCP/Puro) RapidResponse™ Renilla Vector for investigating regions controlling transcription... Pete Stecha 212933 pGL4.82(hRluc/Puro) Renilla Vector for investigating regions controlling transcription...pGL4Luc-RLuc Firefly, Renilla Dual reporter reporter vector allowing the study of biscistronic promoters. Daniel...119760 pSGDlucV3.0 Firefly, Renilla Dual reporter vector. Insertion of 5' promoter/enhancer regions upstream...

...Walker Jackson 78624 sgRNA(MS2)_Prnp_SAM1 PRNP U6 Dementia Walker Jackson 78625 sgRNA(MS2)_Prnp_SAM2 PRNP...Hyman 87200 pX335 HTT sgRNA-b HTT U6 Huntington's Mahmoud Pouladi 87201 pX335 HTT sgRNA-a HTT U6 Huntington's...237005 VCP-HaloTag Vector VCP Halo CMV ALS Promega Corporation 237006 HaloTag-VCP Vector VCP Halo CMV ALS...19 James Trimmer 129409 Slc1a3-CreERT2 Targeting Vector SLC1A3 PGK Episodic ataxia Walker Jackson 129410...His T5 Parkinson's Suzanne Pfeffer 208392 Lenti-sgRNA-Cre-GpNLuc-sgmATM ATM Ataxia telangiectasia Gaorav...ALS Promega Corporation 237148 NanoBRET Assay Vector, TREM2-HaloTag TREM2 Halo CMV Alzheimer's Promega ...Corporation 237172 EIF2AK2(252-551)-NanoLuc Fusion Vector EIF2AK2 NanoLuc CMV VWM disease Promega Corporation...