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We narrowed to 11 results for: single guide rna sgrna

Showing: 1 - 11 of 11 results

  1. Zhang Lab CRISPR Page

    Type
    Collection
    ...with the guide RNA. For most applications, we recommend using Cas9 with the single guide RNA (sgRNA), which...PX330; SpCas9 and single guide RNA 48138 : PX458; SpCas9-2A-EGFP and single guide RNA 62988 : PX459; SpCas9...-2A-Puro and single guide RNA 48873 : PX460; SpCas9n (D10A nickase) and single guide RNA 48140 : PX461...nickase) and single guide RNA 62987 : PX462; SpCas9n-2A-Puro (D10A nickase) and single guide RNA 48137 : PX165...without sgRNA. Full references are below. 1. SpCas9 (or SpCas9n, D10A nickase) + single guide RNA: These... packages both the SaCas9 gene and its single guide RNA (sgRNA) into one plasmid (Ran et al., Nature 2015...promoter-driven; for sgRNA cloning; has GFP-KASH for FACS sorting SaCas9 + single guide RNA: These plasmids...

  2. Lentiviral Prep Service

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    ...ways. Cas9 proteins can be guided to specific genomic sites by an RNA guide (gRNA) that harbors targeting...73178 Human sgRNA library Brunello in lentiGuide-Puro Human sgRNA library in backbone lentiGuide-Puro targeting...73633 Mouse sgRNA library Brie in lentiGuide-Puro Mouse sgRNA library in backbone lentiGuide-Puro containing...Human CRISPRa sgRNA library Calabrese in backbone XPR_502 (P65 HSF), Set A Human CRISPRa sgRNA activation...containing 56,762 unique sgRNAs. This backbone does not contain dCas9. 56,762 unique sgRNAs targeting 18,885 ... Doench , Root 73179 Human gRNA pooled library in lentiCRISPRv2 Human sgRNA library in backbone lentiCRISPRv2...biological processes. For more information, see our Guide to Plasmid Pooled Libraries . ID Name Description...

  3. CRISPR Plasmids - Empty gRNA Vectors

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    ...expression of more sgRNAs in a single vector. sgRNAs are expressed as polycistronic glycine tRNA-gRNA genes (PTGs...Regulation Activate Interfere Epigenetics RNA Targeting RNA Targeting RNA Editing Other Applications Purify Tag...U6 promoters for sgRNA expression. Chen pCFD4-U6:1_U6:3tandemgRNAs Drosophila Single plasmid for the expression...pyogenes Joung sgRNA with U6 promoter 48962 Worm HindIII none S. pyogenes de Bono sgRNA with rpr-1 promoter...SpBsmBI sgRNA Hygro 62205 Mammalian/Lentiviral BsmBI none S. pyogenes Hygro Maehr pLenti SpBsmBI sgRNA Puro...all-in-one expression vectors containing multiple guide RNA expression cassettes and a Cas9 nuclease/nickase...dCas9-VP64 along with 1-4 sgRNAs expressed from independent promoters. sgRNAs are cloned into separate ...

  4. CRISPR Plasmids - C. elegans

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    Collection
    ...Regulation Activate Interfere Epigenetics RNA Targeting RNA Targeting RNA Editing Other Applications Purify Tag...-specific gRNA in the same cell at the same time. Single plasmids containing both the gRNA and Cas protein...Zebrafish Xenopus CRISPR Resources CRISPR Guide Viral Preps Protocols gRNA Design Tools CRISPR Blog Posts The...is often limited to a single category (cut, nick, etc.) On the other hand, gRNA plasmids that do not co-express... (PAM = NGG) 47549 pDD162 (Peft-3::Cas9 + Empty sgRNA) R07E5.16 U6 yes, cut Goldstein 42250 DR274 T7 BsaI...transcription none, need Cas9 plasmid Boxem 47912 SP6-sgRNA-scaffold SP6 AflII In vitro transcription none, ...need Cas9 plasmid Sternberg 177783 L4440_BioBrick-sgRNA T7 BbsI, BsaI Ingestion by C. elegans none, need...

  5. CRISPR Pooled gRNA Libraries

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    ...Regulation Activate Interfere Epigenetics RNA Targeting RNA Targeting RNA Editing Other Applications Purify Tag...Human miRNA CRISPR Knockout Library 112200 Knockout Human Lin 3rd 4-5 8,382 Human Paired-guide RNA (pgRNA...46,354 BARBEKO sgRNA Library 174163 Knockout, Base Editing Human Wei 3rd 3 53,502 sgRNA Pool for Streptococcus...Zebrafish Xenopus CRISPR Resources CRISPR Guide Viral Preps Protocols gRNA Design Tools CRISPR Blog Posts CRISPR...Reduced Double-gRNA Library 137999 Knockout Human Parts 3rd 3 59,576 Human Whole Genome sgRNA iBAR Library...pgRNA) Library for Long Non-coding RNAs (lncRNAs) 89640 Knockout Human Wei 3rd Varies 12,472 pairs HR700...Varies 1,910–3,034 per exon Human Cas13 lncRNA Library 227014 RNA Knockdown Human Sanjana 3rd 6–8 75,184...

  6. CRISPR References and Information

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    ...Cas9 ; pUC gRNA Shuttle PDF, 500 KB Sabatini and Lander gRNA cloning into pLX-sgRNA pLX-sgRNA PDF, 129 ...Addgene's collection of CRISPR plasmids. CRISPR Guide Addgene's guide to using CRISPR plasmids in your lab, from...597 KB Zhang gRNA cloning CRISPR RNA array: Cas9 (pX260) or Cas9 D10A (pX334) ; tracrRNA: Cas9 (pX330)...window) Can be used to identify novel gRNA target sites in a single gene, as well as a target site common...dCas9 PDF, 110 KB Goldstein Nematode: gRNA design and cloning Cas9-sgRNA construct PDF, 364 KB Goldstein Nematode... can identify positively or negatively selected sgRNAs, genes, or pathways. For use with genome-scale ... opens in a new window) for lentiviral screens. sgRNA Scorer 2.0 (Link opens in a new window) A tool that...

  7. CRISPR Plasmids - Drosophila

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    Collection
    ...Regulation Activate Interfere Epigenetics RNA Targeting RNA Targeting RNA Editing Other Applications Purify Tag...base-to-base conversions using a prime editing guide RNA (pegRNA). The pegRNA directs the nickase to the target site...-specific gRNA in the same cell at the same time. Single plasmids containing both the gRNA and Cas protein...Zebrafish Xenopus CRISPR Resources CRISPR Guide Viral Preps Protocols gRNA Design Tools CRISPR Blog Posts The...Nick CRISPR/Cas nickase mutants introduce gRNA-targeted single-strand breaks in DNA instead of the double-strand...is often limited to a single category (cut, nick, etc.) On the other hand, gRNA plasmids that do not co-express...49330 pAc-sgRNA-Cas9 dU6 BspQI Transfection Puromycin yes, cut Ji-Long Liu 45946 pU6-BbsI-chiRNA dU6 BbsI...

  8. CRISPR Guide

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    ...Science Guides CRISPR Guide CRISPR Guide Jump To: CRISPR Guide Overview CRISPR Basics Small Edits Large...components: a guide RNA ( gRNA or sgRNA ) and a CRISPR-associated endonuclease (Cas enzyme). The gRNA is a short...conversions using a prime editing guide RNA ( pegRNA ). Like sgRNA, pegRNA directs the nickase to the target...that target RNA instead of DNA enables tracking of RNA in live cells, including orthogonal RNA/DNA labeling...Koonin, E. V., & Zhang, F. (2015). CPF1 is a single RNA-Guided endonuclease of a Class 2 CRISPR-CAS system...libraries use arrays of multiple gRNAs to target paralogs or lncRNAs . Guide RNAs are designed in silico and...modified scaffold gRNA and additional RNA-binding helper activators; highest levels of single-gene activation...

  9. Empty Backbones - Choosing Your Perfect Plasmid Backbone

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    ...of new shRNA sequences Zeocin®/Bleo Mammalian lenti sgRNA(MS2)_zeo backbone - 3rd gen lenti sgRNA cloning...base-editing plasmid in Pseudomonas species Vectors for RNA-guided genome editing in plants Visit our CRISPR Plasmids...selectable marker pMSCV-U6sgRNA(BbsI)-PGKpuro2ABFP - For retroviral delivery of one sgRNA pMKO.1 GFP - Retroviral...- Deliver sgRNA and Cas9 into plants by Agrobacterium-mediated transformation Dual-sgRNA_hU6-mU6 - Lentiviral...hygromycin resistance pRGEB32 - Express sgRNA/PTG with rice snoRNA U3 promoter and Cas9 with rice ubiquitin..., you have many elements to consider. Here is a guide to a selection of Addgene's empty vector backbones...the final product should be translated as a single string of amino acids that preserves the sequence...

  10. CRISPR History and Development for Genome Engineering

    Type
    Collection
    ...Regev A, Koonin EV, Zhang F. 2015. Cpf1 Is a Single RNA-Guided Endonuclease of a Class 2 CRISPR-Cas System...loci to generate CRISPR RNAs (crRNAs). Through sequence homology, these crRNAs guide a Cas nuclease to the...guanosine. RNA editors : Cas13 fused to the ADAR2 deaminase convert adenosine to inosine on RNA. Inosine...CRISPR-Cas nuclease specificity using truncated guide RNAs. Nat Biotechnol . 32(3):279-84. PMID: 24463574...Weissman JS, Qi LS. 2013. CRISPR-mediated modular RNA-guided regulation of transcription in eukaryotes. Cell... JA, Charpentier E. 2012. A programmable dual-RNA-guided DNA endonuclease in adaptive bacterial immunity... M, DiCarlo JE, Norville JE, Church GM. 2013. RNA-guided human genome engineering via Cas9. Science . ...

  11. CRISPR Plasmids - Tagging

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    Collection
    ...Regulation Activate Interfere Epigenetics RNA Targeting RNA Targeting RNA Editing Other Applications Purify Tag...reaction produces a single plasmid targeting vector that encodes both a guide RNA transcript and a repair...Zebrafish Xenopus CRISPR Resources CRISPR Guide Viral Preps Protocols gRNA Design Tools CRISPR Blog Posts The...unavailable, you will need to design and clone in a gRNA(s) to guide the Cas9 protein to your target sequence, ...an expression cassette for the Cas9-programming sgRNA and HR donors for selectable genome modification... dCas9-FokI Screen Pooled Libraries gRNAs Empty gRNA Vectors gRNAs By Species Mammalian Bacteria Drosophila...expresses Cas9 & PITCh-gRNA pX330A-FBL/PITCh - expresses Cas9, PITCh-gRNA, & FBL-specific gRNA pCRIS-PITChv2-FBL...
Showing: 1 - 11 of 11 results