...genes and containing 76,441 unique sgRNAs along with 1000 non-targeting controls. This backbone does not contain...unique sgRNAs targeting 19,114 human genes along with 1000 non-targeting controls Puromycin Doench , Root 73179...genes and containing 76,441 unique sgRNAs along with 1000 non-targeting controls. This backbone contains SpCas9...unique sgRNAs targeting 19,114 human genes along with 1000 non-targeting controls Puromycin Doench , Root Human... unique sgRNAs targeting 19,674 genes along with 1000 non-targeting controls. This backbone does not contain...unique sgRNAs targeting 19,674 mouse genes along with 1000 non-targeting controls Puromycin Doench , Root Cas9...Cas9 proteins can be guided to specific genomic sites by an RNA guide (gRNA) that harbors targeting sequences...

...sonication to break DNA up into fragments of 300-1000 bps in length. Note: This sonication process may...response has developed, the antibodies are collected, tested against the antigen, packaged, and sold. This is...immortalized B cells). The genetic reorganization that creates variety in the antibody allowing for their extreme...class therefore has a valency, or number of binding sites available per antibody unit, which is typically ...multiplied by two, as each antibody has two binding sites. Isotype Description Configuration and Valency Subclasses...Monomer with a valency of 2 IgE Protects against parasites and is responsible for driving allergic reactions...in the lab. Check for and record any expiration dates. Buffers Buffers are a concern for conjugating and...

...plasmid, are recombined into a DNA molecule that incorporates sequences from both plasmids. This DNA molecule...for the AdenoBuilder genome assembly system; consolidates the regions present in pAd5-B6 ∆E3-GFP and pAd5... Vogelstein 16407 pAdEasy 2-GFP beta-gal Shuttle Test plasmid that contains β-gal and GFP; contains ∼10Kb...in a one-tube reaction. ID Kit Description PI 1000000176 AdenoBuilder toolkit Plasmids contain modular...

...Gene and Genome Editing , 3–4 , 100013. DOI: 10.1016/j.ggedit.2022.100013 Zetsche, B., Gootenberg, J. S....interesting adaptive immune response that detects and eliminates foreign DNA, which scientists have modified for... used for small precision edits. NHEJ directly ligates the break ends without the need for a homologous...large genomic deletions or inversions with two gRNA sites on the same chromosome. Most multiplex systems enable...most gRNA targeting sequences will have additional sites of partial homology throughout the genome, called...Directed Repair (HDR) Figure 4: Overview of HDR HDR generates specific genomic edits by copying from a DNA donor...mutation, while whole plasmid or linear dsDNA templates work better for large edits or insertions. When...

...both the N and C terminus and induces mutation at rates much higher than the parental vectors. pC-GoldyTALEN...Sangamo)-FokI-ELD-Destination Golden Gate TALEN 2.0 1000000030 Yamamoto Lab TALEN Construction and Evaluation... Features include (i) Esp3I (BsmBI) restriction sites for full compatibility with the Golden Gate TALEN... 21179091), as well as (v) distinct selection cassettes on pTAL7a and pTAL7b for enrichment of double-...

...MS2 binding sites within this plasmid. pZE31-DsRed-ms2x2 26248 MS2 coat protein binding sites Philippe Cluzel...protein that activates expression of lacZ in response to green light. BIOFAB plasmid set 1000000037 N/A N/A...strain USO hisB- pyrF- rpoZ- , this promotes growth on plates lacking His and containing 3-AT. FLIParaF....microscopy. pZE31ms2 25860 MS2 coat protein binding sites Philippe Cluzel RNA localization: Contains a 2x ...appropriate cDNA has been fused to the MS2 binding sites within this plasmid. Return to Top Protein Purification...N-terminal GST tag and TEV cleavage site. Includes sites for LIC cloning, and a "stuffer" fragment that includes...mOCR, MBP, with and without SUMO or TEV cleavage sites to remove tags Scott Gradia Arabinose inducible ...

..._CTD_del321-343 TARDBP His T7 ALS Nicolas Fawzi 100092 pET28-his-3C_Tau352 MAPT His T7 Parkinson's, FTD... PRKN mTurquoise2 CMV Parkinson's Pablo Rivera-Fuentes 111723 pBacMam2-DiEx-LIC-N-flag_huntingtin_full-length_Q23...