We narrowed to 5 results for: tracrRNA

1. CRISPR History and Development for Genome Engineering

   Type

   Collection

   ...trans-activating crRNA (tracrRNA) with homology to the short palindromic repeat. The tracrRNA helps recruit RNAse...together separate the individual crRNAs. (3) The tracrRNA and Cas9 nuclease form a complex with each individual...individual, unique crRNA. (4) Each crRNA:tracrRNA:Cas9 complex seeks out the DNA sequence complementary...cleaves both strands near the PAM. (6) The crRNA:tracrRNA:Cas9 complex unbinds after the double-strand break...

2. Zhang Lab CRISPR Page

   Type

   Collection

   ...CRISPR RNA (crRNA) and the trans-activating crRNA (tracrRNA; Hsu et al ., Nature Biotechnology 2013). We also... cassettes to express the CRISPR RNA array and tracrRNA separately (Cong et al ., Science 2013). Additionally...or SpCas9n, D10A nickase) + CRISPR RNA array + tracrRNA: This plasmid contains three expression cassettes...

3. Bikard Lab - CRISPR Repression Collection

   Type

   Collection

   ...repeat motifs (squares), and also carrying the tracrRNA sequence (not shown). (B) Relative GFP and RFP...

4. CRISPR References and Information

   Type

   Collection

   ...RNA array: Cas9 (pX260) or Cas9 D10A (pX334) ; tracrRNA: Cas9 (pX330) or Cas9 D10A (pX335) PDF, 255 KB...

5. CRISPR Guide

   Type

   Collection

   ...synthetic fusion of the endogenous bacterial crRNA and tracrRNA that provides both targeting specificity and scaffolding...