We narrowed to 137 results for: tracrRNA

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pMK-AttL1-NotI/BamHI-U6-BsaI-tracrRNAopt-BamHI-U6-SapI-tracrRNAopt-NotI/XbaI-7sk-BsmBI-tracrRNA-XbaI-AttL2

Plasmid

#190898

Purpose

Entry vector for multiple sgRNA cloning

Depositor

Nicole Déglon

Article

Duarte et al Mol Ther Methods Clin Dev. 2023 Mar 27;2

Type

Empty backbone

Use

CRISPR

Expression

Bacterial and Mammalian

Promoter

U6 and 7sk

Available Since

March 27, 2023

Availability

Academic Institutions and Nonprofits only

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pRS426-SNR52-tracrRNA

Plasmid

#251985

Purpose

Expression of tracrRNA for editing, used together with crRNA Plasmid #251984. This plasmid is also called tracrRNA in the paper.

Depositor

Alice Ting

Article

Yin et al Proc Natl Acad Sci U S A. 2026 Jan 20;12

Insert

tracrRNA

Use

CRISPR

Expression

Yeast

Promoter

SNR52

Available Since

April 30, 2026

Availability

Academic Institutions and Nonprofits only

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pU6-BoxB-petracrRNA

Plasmid

#207625

Purpose

Expresses BoxB-petracrRNA in mammalian cells (with a human-U6 promoter)

Depositor

Jay Shendure

Article

Choi et al Elife. 2025 Feb 12;13:RP98110. doi: 10.7

Insert

BoxB-petracrRNA

Use

Synthetic Biology

Promoter

hU6

Available Since

Feb. 10, 2025

Availability

Academic Institutions and Nonprofits only

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pUC-tracrRNA-PMtU6-26

Plasmid

#209191

Purpose

Cloning of sgRNAs for expression in plants

Depositor

Christian Staehelin

Insert

MtU6-26 SnRNA promoter

Use

Cloning vector

Available Since

Jan. 12, 2024

Availability

Academic Institutions and Nonprofits only

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pUC-tracrRNA-PMtU6-1

Plasmid

#209190

Purpose

Cloning of sgRNAs for expression in plants

Depositor

Christian Staehelin

Insert

MtU6-1 SnRNA promoter

Use

Cloning vector

Available Since

Jan. 12, 2024

Availability

Academic Institutions and Nonprofits only

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tracrRNA(scr)-CJ8421_04975 mRNA

Plasmid

#199718

Purpose

Expresses the Campylobacter mRNA CJ8421_04975 and tracrRNA with scrambled anti-repeat sequencesequence

Depositor

Chase Beisel

Article

Jiao et al Nat Biotechnol. 2023 Jan 5. doi: 10.1038

Insert

CJ8421_04975 mRNA and scrambled tracrRNA

Use

CRISPR

Expression

Bacterial

Available Since

May 23, 2023

Availability

Academic Institutions and Nonprofits only

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lentiGuide-Puro-Chen-tracrRNA_2xBsmBI

Plasmid

#196710

Purpose

Backbone for cloning single guides (Chen tracrRNA PMID: 24360272) or guide libraries with untranscribed barcodes and a tracrRNA of choice

Depositor

Bernhard Schmierer

Article

Schmierer et al Mol Syst Biol. 2017 Oct 9;13(10):945. do

Type

Empty backbone

Use

CRISPR and Lentiviral

Available Since

Sept. 28, 2023

Availability

Academic Institutions and Nonprofits only

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Topo_SpCas9.tracrRNA-CS1_AsCas12a DRv10

Plasmid

#237553

Purpose

TOPO vector for the cloning of the SpCas9 tracrRNA-CS1 - AsCas12a Direct Repeat (DR) v10 fragment into the pLCHKO_v4 hgRNA vector for scCHyMErA-Seq experiments.

Depositor

Thomas Gonatopoulos-Pournatzis

Article

Kumari et al Nat Commun. 2026 Feb 3;17(1):1218. doi:

Insert

SpCas9.tracrRNA-CS1_AsCas12a DRv10

Use

CRISPR

Available Since

Jan. 12, 2026

Availability

Academic Institutions and Nonprofits only

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pEJS2194-RP_PiggyBac_SpCas9ABE8e-tracrRNA-Puro

Plasmid

#211821

Purpose

PiggyBac vector expressing SpCas9-ABE8e with tracrRNA for stable cell line establishment

Depositor

Erik Sontheimer

Article

Zhang et al Nucleic Acids Res. 2023 Nov 30:gkad1125.

Insert

SpCas9-ABE8e, tracrRNA and Puromycin resistance gene

Expression

Mammalian

Promoter

chicken β-actin promoter, U6 promoter and hPGK pr…

Available Since

Jan. 10, 2024

Availability

Academic Institutions and Nonprofits only

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pEJS-HZ08-pAAVsc.U6-tracrRNA

Plasmid

#211816

Purpose

AAV vector expressing tracrRNA under U6 promoter

Depositor

Erik Sontheimer

Article

Zhang et al Nucleic Acids Res. 2023 Nov 30:gkad1125.

Insert

U6-tracrRNA

Use

AAV

Expression

Mammalian

Promoter

U6

Available Since

Jan. 10, 2024

Availability

Academic Institutions and Nonprofits only

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pX097-U6-SpTracrRNA-EF1a-hSpRNaseIII-mCherry

Plasmid

#41863

Purpose

This plasmid is used to reconstitute the complete Type II CRISPR system from S. pyogenes and contains the host factor RNaseIII and tracrRNA.

Depositor

Feng Zhang

Article

Cong et al Science. 2013 Jan 3.

Insert

U6-SpTracrRNA-EF1a-hSpRNaseIII-mCherry

Use

CRISPR and Lentiviral

Expression

Mammalian

Available Since

Feb. 27, 2013

Availability

Academic Institutions and Nonprofits only

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pEJS-HZ96 AAV-SpABE8e-N-terminus_tracrRNA

Plasmid

#211817

Purpose

AAV vector expressing N-terminal of SpCas9-ABE8e and tracrRNA

Depositor

Erik Sontheimer

Article

Zhang et al Nucleic Acids Res. 2023 Nov 30:gkad1125.

Insert

N-terminus of SpCas9-ABE8e and tracrRNA

Use

AAV

Expression

Mammalian

Promoter

chicken β-actin promoter and U6 promoter

Available Since

Jan. 10, 2024

Availability

Academic Institutions and Nonprofits only

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pEJS-HZ100 AAV-SpABE8e-C_terminus-tracrRNA

Plasmid

#211818

Purpose

AAV vector expressing C-terminus of SpCas9-ABE8e with tracrRNA

Depositor

Erik Sontheimer

Article

Zhang et al Nucleic Acids Res. 2023 Nov 30:gkad1125.

Insert

C-terminus of SpCas9-ABE8e and tracrRNA

Use

AAV

Expression

Mammalian

Promoter

chicken β-actin promoter and U6 promoter

Available Since

Jan. 10, 2024

Availability

Academic Institutions and Nonprofits only

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pEJS1553 pLenti-U1a-SpCas9-miniU6-tracrRNA

Plasmid

#211819

Purpose

lentiviral vector expressing SpCas9 and tracrRNA for stable cell line extablishment

Depositor

Erik Sontheimer

Article

Zhang et al Nucleic Acids Res. 2023 Nov 30:gkad1125.

Insert

SpCas9, tracrRNA and blasticidin resistance gene

Use

Lentiviral

Expression

Mammalian

Promoter

U1a promoter and miniU6 promoter

Available Since

Jan. 10, 2024

Availability

Academic Institutions and Nonprofits only

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pTO136_pADH1-tRNA-Ala-gRNA-tracrRNA-HDV-tAgTEF2

Plasmid

#171104

Purpose

Expresses sgRNA (ENO1 gRNA) in Candida auris.

Depositor

Teresa O'Meara

Article

Santana et al Nat Commun. 2021 Dec 10;12(1):7197. doi:

Insert

sgRNA

Expression

Yeast

Promoter

ADH1

Available Since

July 12, 2021

Availability

Academic Institutions and Nonprofits only

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pEJS-HZ03-pLenti-U1a-mSpCas9-U6-tracrRNA-PuroR

Plasmid

#211815

Purpose

lentiviral vector expressing SpCas9 and tracrRNA for stable cell line establishment

Depositor

Erik Sontheimer

Article

Zhang et al Nucleic Acids Res. 2023 Nov 30:gkad1125.

Insert

SpCas9, tracrRNA and puromycin selection gene

Use

Lentiviral

Expression

Mammalian

Promoter

U1a promoter, U6 promoter, and hPGK promoter

Available Since

Jan. 10, 2024

Availability

Academic Institutions and Nonprofits only

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T7m

Plasmid

#121030

Purpose

MoClo golden gate assembly DE part for tracrRNA terminator (natural terminator from S. pyogenes CRISPR-system tracrRNA). Please see Supplemental Documents for annotated Genbank file.

Depositor

Richard Murray

Insert

S. pyogenes tracrRNA terminator

Use

Synthetic Biology

Available Since

Dec. 4, 2019

Availability

Academic Institutions and Nonprofits only

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pLY273

Plasmid

#184155

Purpose

Reprogrammed short tracrRNA for hijacking mRNA of zraP

Depositor

Baojun Wang

Article

Liu et al Nat Commun. 2022 Apr 11;13(1):1937. doi:

Insert

s-tracrRNA-zraP-22

Use

Synthetic Biology

Expression

Bacterial

Promoter

araBAD promoter

Available Since

Oct. 31, 2022

Availability

Academic Institutions and Nonprofits only

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pLY232

Plasmid

#184149

Purpose

short tracrRNA generator with WT anti-repeat region

Depositor

Baojun Wang

Article

Liu et al Nat Commun. 2022 Apr 11;13(1):1937. doi:

Insert

s-tracrRNA-WT

Use

Synthetic Biology

Expression

Bacterial

Promoter

araBAD promoter

Available Since

Nov. 9, 2022

Availability

Academic Institutions and Nonprofits only

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pLY283

Plasmid

#184156

Purpose

Reprogrammed short tracrRNA for hijacking mRNA of zntA

Depositor

Baojun Wang

Article

Liu et al Nat Commun. 2022 Apr 11;13(1):1937. doi:

Insert

s-tracrRNA-zntA-1034

Use

Synthetic Biology

Expression

Bacterial

Promoter

araBAD promoter

Available Since

Oct. 31, 2022

Availability

Academic Institutions and Nonprofits only

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