We narrowed to 5 results for: cas9 expression construct

...Yeast Expression, Insect Expression Chen Davidovich 41 plasmids to construct multigene expression vectors...assembly of CRISPR/Cas9 constructs for use in plants. The constructs are made with zCas9i, a high efficiency...multi-gene constructs for S. cerevisiae expression. Multiplex Yeast Toolkit (MYT) Yeast Expression, CRISPR...KTK) Bacterial Expression Tom Ellis DNA parts to create multigene constructs expressed in the cellulose-producing...4G-Cloning Plasmid Kit Bacterial Expression, Insect Expression, Mammalian Expression Stephan Gruber Platform for... Mammalian Expression Takashi Yamamoto Plasmids for construction of all-in-one CRISPR/Cas9 vectors expressing...Assembly Kit Other, Yeast Expression, Plant Expression, Bacterial expression Christopher Dupont 95 plasmids...

...dsDNA construct flanked by ITRs. Cells from the packaging HEK293 or HER911 cell lines expressing E1 are...pAdTrack series contains an IRES-GFP construct that enables co-expression of GFP with the transgene of interest...transgenes to express for a shorter period of time or when needing to package larger cargo. CRISPR-Cas9 Delivery...case of some commonly used Cas9 homologs, especially when having to express them alongside a guide RNA...capacity is large enough to carry the Cas9 gene and a gRNA expression cassette in one single viral particle...L2, L3, L4, L5) regions. Early transcripts are expressed shortly after the virus enters the host cell, ... host cell manipulation. Late transcripts are expressed after DNA replication starts and primarily encode...

...the host genome can have on transgene expression and the expression of neighboring genes. rAAV episomes ...Addgene. CRISPR/Cas9 Delivery rAAV vectors are a popular choice for the delivery of CRISPR/Cas9 elements, making... sequence overlap. Co-expression induces homologous recombination and expression of the full-length transgene... genome editing. Packaging the Cas9 from Streptococcus pyogenes (SpCas9) and a gRNA together into an rAAV...this obstacle, has been to use smaller Cas9 orthologs, such as SaCas9 from Staphylococcus aureus . The Feng...splice together, reconstructing the full-length transgene that can then be expressed. This method allows...deliver genetic cargo into cells. Recombinant AAV constructs are designed by replacing the Rep and Cap regions...

... available with multiple Cas9 variants, including high fidelity Cas9s, Cas9s with various PAM requirements...to either co-infect with a Cas9-producing lentivirus or use a Cas9-expressing stable cell line. After applying...strand SpCas9-HF1 — disrupt Cas9’s interactions with DNA phosphate backbone HypaCas9 — increase Cas9 proofreading...more Cas9 fusion tools later in the guide. Figure 3: Overview of Cas9n. Increasing fidelity of Cas9 Researchers...enhanced specificity by engineering Cas9 into high fidelity Cas9s (hfCas9) . There is currently no defined...application. Cas9 Orthologs While SpCas9 is the most commonly used Cas enzyme, additional Cas9 orthologs ...Additionally, constructs with Cas9 fused to proteins that modulate HDR or NHEJ components, like miCas9 , Cas9...

...target non-dividing cells, enable stable long-term expression in a host, and exhibit low immunogenicity. This...second-generation packaging system because transgene expression from the LTR is Tat-dependent. Figure 3: Second-generation...) can be used in transient transfections, but expression will be greatly limited due to the LTRs. Generally...be used in direct transfections for transient expression of a transgene. For more detailed protocols, ... VSV-G is toxic under long-term, constitutive expression. In order to make stable cell lines, VSV-G is...Vectors Due to their integration and long-term expression of a transgene, lentiviral vectors are useful...fluorescence-activated cell sorting (FACS) is used to sort cells expressing GFP and later expanded into a cell line. More...