... available with multiple Cas9 variants, including high fidelity Cas9s, Cas9s with various PAM requirements...to either co-infect with a Cas9-producing lentivirus or use a Cas9-expressing stable cell line. After applying...strand SpCas9-HF1 - disrupt Cas9’s interactions with DNA phosphate backbone HypaCas9 - increase Cas9 proofreading...new tools. Figure 3: Overview of Cas9n Nickase and dead Cas9 The two Cas9 nuclease domains, RuvC and HNH...enhanced specificity by engineering Cas9 into high fidelity Cas9s (hfCas9) . There is currently no defined...application. Cas9 Orthologs While SpCas9 is the most commonly used Cas enzyme, additional Cas9 orthologs ...Additionally, constructs with Cas9 fused to proteins that modulate HDR or NHEJ components, like miCas9 , Cas9...

...can then be expressed. This method allows for longer transgene expression, but expression is much less...integration can affect the expression of transgenes, as well as the expression of neighboring genes. The... sequence overlap. Co-expression induces homologous recombination and expression of the full-length transgene...cycle; VP1, VP2, VP3 - capsid proteins). When constructing an AAV transfer plasmid, the transgene is placed...plasmids available from Addgene. The popular CRISPR/Cas9 genome editing system has been modified for use ...Episomal stability enables long-term transgene expression in non-dividing cells and is a key advantage ...synthesize the complementary strand, transgene expression may be delayed. To overcome this rate-limiting...