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We narrowed to 5 results for: ccdb gene

Showing: 1 - 5 of 5 results

  1. Gamma-Retroviral Vector Guide

    Type
    Guide
    ...contains a Gateway cassette containing the ccdB gene, a ccdB-resistant strain is necessary. For more information...the necessary packaging genes, while lentiviruses also contain accessory genes specific to each virus ...common packaging genes gag , pol , and env . The LTRs flank all of these required genes, and anything in...— contains packaging genes gag and pol Envelope plasmid — contains packaging gene env ; usually VSV-G ... envelope gene, a process called pseudotyping. VSV-G is by far the most common envelope gene, as it has...commonly used in gene therapy applications to more specifically direct delivery of gene therapies. Common...gamma-retroviral vectors. Gene Therapy Gamma-retroviral vectors are a popular choice in gene therapy applications...

  2. Adenovirus Guide

    Type
    Guide
    ...contains a Gateway cassette containing the ccdB gene, a ccdB-resistant strain is necessary. For more information...provide a large transgene packaging capacity. Large protein-coding genes and complex gene expression cassettes...(2017). Adenovirus-mediated gene delivery: potential applications for gene and cell-based therapies in..., subsequent generations of rAdV vectors have been developed where more adenoviral genes have been removed...containing the adenoviral genes necessary for viral production. In AdEasy™, the transgene of interest is cloned...containing the transgene of interest, and the adenoviral plasmid containing the adenoviral genes necessary ...second and third-generation vectors. This capacity is large enough to carry the Cas9 gene and a gRNA expression...

  3. Lentiviral Vector Guide

    Type
    Guide
    ...strand of RNA contains packaging genes, regulatory genes, accessory genes, and the long-terminal repeats...contains a Gateway cassette containing the ccdB gene, a ccdB-resistant strain is necessary. For more information...shRNA , and overexpressing of oncogenes and other disease-associated genes. Gene Therapy Due to their relative...complex genomes, containing packaging genes and accessory genes specific to each virus type, while retroviruses...require the regulatory genes tat and rev , along with virus-specific accessory genes (for example, vif , ...Packaging plasmid — contains packaging genes gag and pol , and regulatory genes tat and rev ; separated into two... envelope gene, a process called pseudotyping. VSV-G is by far the most common envelope gene, as it has...

  4. Adeno-associated virus (AAV) Guide

    Type
    Guide
    ...contains a Gateway cassette containing the ccdB gene, a ccdB-resistant strain is necessary. For more information...capacity for long-term gene expression. These features make them ideal for gene therapy, neuroscience ...biomedical fields. Gene Therapy One of the major applications of rAAV vectors is in the field of gene therapy to...virus (AAV) as novel vectors for human gene therapy . Current Gene Therapy, 3 (4), 281–304. https://doi....the two ITRs with a gene expression cassette that typically contains the transgene of interest. The size...vectors to deliver genes to cells in in vivo models. For more detailed protocols, see Addgene’s Viral Vector...host genome can have on transgene expression and the expression of neighboring genes. rAAV episomes can develop...

  5. Molecular Biology Reference

    Type
    Guide
    ...bacterial populations, an antibiotic resistance gene (i.e., a gene whose product confers resistance to ampicillin...transcription. Gene Knock-down Plasmids - Used for reducing the expression of an endogenous gene. This is frequently...chromosomes down to study their genes, marking the birth of molecular genetics. Today, scientists can easily...easily study and manipulate genes and other genetic elements using specifically engineered plasmids, commonly...when a gene is inserted within the MCS, its expression will be driven by the promoter. As a general rule...the gene, promoter, or other DNA fragment cloned into the MCS. The insert is typically the genetic element...experiment, see Addgene's empty backbone page for more information. Expression Plasmids - Used for gene expression...
Showing: 1 - 5 of 5 results