...Transcription is tightly controlled in both bacteria and eukaryotes. Promoters are controlled by various DNA regulatory...vital component of expression vectors because they control the binding of RNA polymerase to DNA. RNA polymerase...a functional protein. Thus the promoter region controls when and where in the organism your gene of interest... are proteins which recruit RNA polymerase and control and regulate the transcription of DNA into mRNA...consists of the sequence TTGACA and this element controls the rate of transcription. Bacterial cells contain...Operons are a cluster of different genes that are controlled by a single promoter and operator. Operons are...operon in bacteria is an example of a negatively controlled operon. A positive repressible operon works in...

...libraries, each plasmid contains a unique cDNA. In shRNA or gRNA libraries, each plasmid contains a unique... Perform Next Generation Sequencing (NGS) on a control sample (no selection) In parallel, apply library...cells Compare NGS results between experimental and control cells Generate a list of gRNAs that disappear with...selection while the other serves as a non-selected control. These two populations are then sequenced using...Screening Pooled CRISPR Libraries Offer Genome-Wide Control for Large-Scale Functional Screens New Tool for...

...that the antibiotic resistance gene is under the control of a bacterial promoter, and is thus expressed ...strength of the promoter is also important for controlling the level of insert expression (i.e., a strong...antibiotic resistance gene (this time, under the control of a non-bacterial promoter) or a fluorescent protein...frequently accomplished through expression of an shRNA targeting the mRNA of the gene of interest. These...plasmids are sequenced with NGS during our quality control process . NGS allows us to sequence entire plasmids...