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We narrowed to 8 results for: crispr library

Showing: 1 - 8 of 8 results

  1. Guide to Using Pooled Libraries

    Type
    Guide
    ...most popular libraries! Genome-wide Screening Using CRISPR/Cas9 Lentiviral CRISPR Libraries Enable Genome-Scale...Amplifying and Using the Library Once you receive your library from Addgene, check the library information to see...this creates a pooled lentiviral CRISPR library. Apply pooled library to cells. DIVERGE: Positive and ...Overview of what pooled libraries are; how to amplify and use a pooled library; and what types of screens... Science Guides Guide to Plasmid Pooled Libraries Plasmid Pooled Libraries Jump To: ...: Pooled Library Guide Introduction Library Amplification Types of Screens Resources An Introduction to... to Pooled Libraries Pooled libraries represent a powerful tool for forward genetic screening, or identifying...

  2. CRISPR Guide

    Type
    Guide
    ...screening using CRISPR libraries What Are Pooled Lentiviral CRISPR Libraries? Pooled lentiviral CRISPR libraries...gene, so CRISPR libraries contain thousands of unique gRNAs. gRNA design for CRISPR libraries is usually...post on lentiviral pooled libraries . Browse Libraries: CRISPR Pooled Libraries Cas9 Fusion Tools dCas9 cannot...Guide CRISPR Guide Jump To: CRISPR Guide Overview CRISPR Basics Small Edits Large Edits Genome-Wide Screening...Figure 7: Overview of CRISPR transposases Browse Plasmids: CRISPR transposases CRISPR Recombinases and Integrases...genome-wide screens using CRISPR involves pooled lentiviral CRISPR libraries. Figure 8: Overview of genome-wide...Non-mammalian CRISPR libraries are also available, such as those for yeast or drosophila . Although CRISPR has ...

  3. Science Guides

    Type
    Guide
    ... below. Antibodies Chemogenetics CRISPR Optogenetics Pooled Libraries Viral Vectors Antibodies Produced...Read More CRISPR Class 2 C lustered R egularly I nterspaced S hort P alindromic R epeat (CRISPR) systems...background information on CRISPR and the basics for planning your first CRISPR experiment. Read More Optogenetics...understand their functions. Read More Pooled Libraries Pooled libraries are single preparations of many different...given library have the same backbone, but they express or target different genes. Some libraries cover...

  4. Modular Cloning Guide

    Type
    Guide
    ...multiplexed CRISPR editing, and more. Fungal Toolkit for Modular Cloning (FTK) Fungal Expression, CRISPR Arnold...experiments. Multiplex CRISPR/Cas9 Assembly Systems Genome Engineering, CRISPR, Mammalian Expression Takashi...MoClo-compatible CRISPR/Cas9 cloning kit with intronized Cas9 Genome Engineering, CRISPR Sylvestre Marillonnet...SpCas9-coding gene. MoClo CRISPR/Cas Toolkit for Plants Genome Engineering, CRISPR, Plant Expression Vladimir...Engineering, CRISPR, Plant Expression Johannes Stuttmann Plasmids for assembly of constructs for CRISPR genome...Genome Engineering, CRISPR, Yeast Expression Brigitte Gasser Plasmids to perform CRISPR/Cas9 mediated genome...(a.k.a. K. phaffii ). NT-CRISPR Plasmid Collection Genome Engineering, CRISPR, Bacterial Expression Anke...

  5. Addgene's eBook Collection

    Type
    Guide
    ...Close About Overlay Select CRISPR 101 January 2021 | Third Edition About CRISPR 101 January 2021 | ...planning your CRISPR experiment and designing your gRNA and how to use pooled libraries in genome-wide...Overlay Select CRISPR 101 en español January 2024 | First Edition, trans. About CRISPR 101 en español...traducción al español de nuestro libro electrónico, CRISPR 101. Close About Overlay Select Antibodies 101 ...

  6. Lentiviral Vector Guide

    Type
    Guide
    ... has an extensive collection of lentiviral CRISPR libraries, including knockout, inhibition/activation...base editing. Browse Addgene's collection of CRISPR libraries . Cellular Barcoding Much like genomic screens...Addgene's collection of in-stock lentiviral preps . CRISPR Genomic Screens Genome-wide screens are an efficient... Inheritance of the barcodes makes lentiviral libraries popular for lineage tracing experiments and monitoring...tumors. Browse Addgene's collection of barcoding libraries . Mouse Modeling Since the development of safer...Popular uses include Cre-lox mice, knockouts using CRISPR or shRNA , and overexpressing of oncogenes and ...Doench, J. G., & Zhang, F. (2013). Genome-Scale CRISPR-CAS9 knockout screening in human cells. Science...

  7. Molecular Biology Reference

    Type
    Guide
    ...editing is most commonly accomplished using CRISPR technology. CRISPR is composed of a DNA endonuclease and ...the genome. For more information on CRISPR check out Addgene’s CRISPR guide . Reporter Plasmids - Used for...techniques and tips! Addgene Protocols Addgene Video Library...

  8. Adeno-associated virus (AAV) Guide

    Type
    Guide
    ... Addgene. CRISPR/Cas9 Delivery rAAV vectors are a popular choice for the delivery of CRISPR/Cas9 elements...of interest has been to create and test capsid libraries with random mutations or insertions in the Cap...making it one of the most common methods for in vivo CRISPR-based genome editing. Packaging the Cas9 from Streptococcus...blog post on overcoming AAV size limitation for CRISPR delivery for more information. Split AAVs to increase...
Showing: 1 - 8 of 8 results