...light-emitting luciferase and a light-sensing optogenetic element. When the luciferase substrate (luciferin... Dlx Interneurons EF1a, CAG General expression Targeted expression. Depending on the experiment, an AAV-encoded...cell-specific expression of AAV-encoded chemogenetic receptors. FLEx vectors block expression in the absence...luciferin) is added, the luciferase enzyme generates light that activates the opsin. The opsin may excite...excite or inhibit the neurons expressing the LMO, depending on its biophysical properties. While LMOs provide...Coupling brighter bioluminescent modules, such as luciferase-fluorescent protein fusions, to more-sensitive...increased efficiencies. Depending on the type of luciferase used, the luciferin substrate may be the native...

...information. Expression Plasmids - Used for gene expression (for the purposes of gene study). Expression vectors... the level of insert expression (i.e., a strong promoter directs high expression, whereas weaker promoters...amount of protein or RNA produced. Expression vectors can drive expression in various cell types (mammalian...reducing the expression of an endogenous gene. This is frequently accomplished through expression of an shRNA... control of a bacterial promoter, and is thus expressed in the bacteria by bacterial transcriptional machinery...restriction enzymes digestion and ligation. In expression plasmids, the MCS is often located downstream...that when a gene is inserted within the MCS, its expression will be driven by the promoter. As a general ...