... ( pegRNA ). Like sgRNA, pegRNA directs the nickase to the target site by homology to a genomic DNA locus...increased efficiency; an additional sgRNA directs Cas9n to a nearby site, driving mismatch repair using the ... CaS-Transposon system for programmable and Site-Directed DNA transpositions. The CRISPR Journal . 2 (... or inversions with two gRNA sites on the same chromosome. Most multiplex systems enable researchers to...101: Homology directed repair CRISPR 101: Validating your genomic edit CRISPR 101: Multiplex expression ...continue to anneal to the target DNA in a 3′ to 5′ direction. The location of any potential mismatches matters... the less efficient but high-fidelity homology directed repair (HDR) pathway. HDR relies on copying DNA...