...destination vector (in this case the destination vector would be the lentiviral transfer vector). The destination... using the popular cloning vector pLKO.1. Trono Lab Resources: Lentivectors Toolbox and Maps Standard ...insert of interest from its parent vector and ligating into a second vector in such as way that the insert...later be digested from this new vector and cloned into the lentiviral vector. This is basically shuffling...between vectors until the gene of interest is flanked by sites compatible with those in the vector into ...site (MCS) from a separate vector into the single site in the lentiviral vector and generate more useful...by the design of the vectors and by safe laboratory practice. In terms of vector design, 2nd and 3rd generation...